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Protein

Non-specific ribonucleoside hydrolase RihC

Gene

rihC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes both purine and pyrimidine ribonucleosides with a broad-substrate specificity with decreasing activity in the order uridine, xanthosine, inosine, adenosine, cytidine, guanosine.

Enzyme regulationi

Subject to catabolite repression.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei233 – 2331By similarity

GO - Molecular functioni

  • adenosine nucleosidase activity Source: EcoCyc
  • inosine nucleosidase activity Source: EcoCyc
  • ribosylpyrimidine nucleosidase activity Source: EcoCyc
  • uridine nucleosidase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:EG11082-MONOMER.
ECOL316407:JW0028-MONOMER.
MetaCyc:EG11082-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Non-specific ribonucleoside hydrolase RihC (EC:3.2.-.-)
Alternative name(s):
Purine/pyrimidine ribonucleoside hydrolase
Gene namesi
Name:rihC
Synonyms:yaaF
Ordered Locus Names:b0030, JW0028
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11082. rihC.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304Non-specific ribonucleoside hydrolase RihCPRO_0000206831Add
BLAST

Proteomic databases

PaxDbiP22564.

Interactioni

Protein-protein interaction databases

BioGridi4261497. 10 interactions.
DIPiDIP-11157N.
IntActiP22564. 2 interactions.
MINTiMINT-1268238.
STRINGi511145.b0030.

Structurei

3D structure databases

ProteinModelPortaliP22564.
SMRiP22564. Positions 3-300.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IUNH family. RihC subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105DU0. Bacteria.
COG1957. LUCA.
HOGENOMiHOG000218839.
InParanoidiP22564.
KOiK12700.
OMAiIKRLVMM.
PhylomeDBiP22564.

Family and domain databases

Gene3Di3.90.245.10. 1 hit.
HAMAPiMF_01432. Nucleosid_hydro_RihC. 1 hit.
InterProiIPR015910. I/U_nuclsd_hydro_CS.
IPR023186. Inosine/uridine_hydrolase.
IPR001910. Inosine/uridine_hydrolase_dom.
IPR022976. Nucleosid_hydro_RihC_nonspecif.
[Graphical view]
PANTHERiPTHR12304. PTHR12304. 1 hit.
PfamiPF01156. IU_nuc_hydro. 1 hit.
[Graphical view]
SUPFAMiSSF53590. SSF53590. 1 hit.
PROSITEiPS01247. IUNH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22564-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLPIFLDTD PGIDDAVAIA AAIFAPELDL QLMTTVAGNV SVEKTTRNAL
60 70 80 90 100
QLLHFWNAEI PLAQGAAVPL VRAPRDAASV HGESGMAGYD FVEHNRKPLG
110 120 130 140 150
IPAFLAIRDA LMRAPEPVTL VAIGPLTNIA LLLSQCPECK PYIRRLVIMG
160 170 180 190 200
GSAGRGNCTP NAEFNIAADP EAAACVFRSG IEIVMCGLDV TNQAILTPDY
210 220 230 240 250
LSTLPQLNRT GKMLHALFSH YRSGSMQSGL RMHDLCAIAW LVRPDLFTLK
260 270 280 290 300
PCFVAVETQG EFTSGTTVVD IDGCLGKPAN VQVALDLDVK GFQQWVAEVL

ALAS
Length:304
Mass (Da):32,561
Last modified:August 1, 1991 - v1
Checksum:i0E98D78DF5C6F70F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54945 Genomic DNA. Translation: CAA38708.1.
U00096 Genomic DNA. Translation: AAC73141.1.
AP009048 Genomic DNA. Translation: BAB96599.1.
PIRiJE0404.
RefSeqiNP_414571.1. NC_000913.3.
WP_001239142.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73141; AAC73141; b0030.
BAB96599; BAB96599; BAB96599.
GeneIDi944796.
KEGGiecj:JW0028.
eco:b0030.
PATRICi32115151. VBIEscCol129921_0027.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54945 Genomic DNA. Translation: CAA38708.1.
U00096 Genomic DNA. Translation: AAC73141.1.
AP009048 Genomic DNA. Translation: BAB96599.1.
PIRiJE0404.
RefSeqiNP_414571.1. NC_000913.3.
WP_001239142.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP22564.
SMRiP22564. Positions 3-300.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261497. 10 interactions.
DIPiDIP-11157N.
IntActiP22564. 2 interactions.
MINTiMINT-1268238.
STRINGi511145.b0030.

Proteomic databases

PaxDbiP22564.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73141; AAC73141; b0030.
BAB96599; BAB96599; BAB96599.
GeneIDi944796.
KEGGiecj:JW0028.
eco:b0030.
PATRICi32115151. VBIEscCol129921_0027.

Organism-specific databases

EchoBASEiEB1074.
EcoGeneiEG11082. rihC.

Phylogenomic databases

eggNOGiENOG4105DU0. Bacteria.
COG1957. LUCA.
HOGENOMiHOG000218839.
InParanoidiP22564.
KOiK12700.
OMAiIKRLVMM.
PhylomeDBiP22564.

Enzyme and pathway databases

BioCyciEcoCyc:EG11082-MONOMER.
ECOL316407:JW0028-MONOMER.
MetaCyc:EG11082-MONOMER.

Miscellaneous databases

PROiP22564.

Family and domain databases

Gene3Di3.90.245.10. 1 hit.
HAMAPiMF_01432. Nucleosid_hydro_RihC. 1 hit.
InterProiIPR015910. I/U_nuclsd_hydro_CS.
IPR023186. Inosine/uridine_hydrolase.
IPR001910. Inosine/uridine_hydrolase_dom.
IPR022976. Nucleosid_hydro_RihC_nonspecif.
[Graphical view]
PANTHERiPTHR12304. PTHR12304. 1 hit.
PfamiPF01156. IU_nuc_hydro. 1 hit.
[Graphical view]
SUPFAMiSSF53590. SSF53590. 1 hit.
PROSITEiPS01247. IUNH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIHC_ECOLI
AccessioniPrimary (citable) accession number: P22564
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: September 7, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Strictly specific for ribonucleosides.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.