P22564 (RIHC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-specific ribonucleoside hydrolase rihC
EC=3.2.-.-

Alternative name(s):
Purine/pyrimidine ribonucleoside hydrolase

Gene names

Name:	rihC
Synonyms:	yaaF
Ordered Locus Names:	b0030, JW0028

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes both purine and pyrimidine ribonucleosides with a broad-substrate specificity with decreasing activity in the order uridine, xanthosine, inosine, adenosine, cytidine, guanosine. HAMAP MF_01432
Enzyme regulation	Subject to catabolite repression. HAMAP MF_01432
Miscellaneous	Strictly specific for ribonucleosides. HAMAP MF_01432
Sequence similarities	Belongs to the IUNH family. RihC subfamily.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	hydrolase activity, acting on glycosyl bonds Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Non-specific ribonucleoside hydrolase rihC HAMAP MF_01432		PRO_0000206831
Sites
	Active site	233	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P22564-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 0E98D78DF5C6F70F	FASTA	304	32,561
	10 20 30 40 50 60 MRLPIFLDTD PGIDDAVAIA AAIFAPELDL QLMTTVAGNV SVEKTTRNAL QLLHFWNAEI 70 80 90 100 110 120 PLAQGAAVPL VRAPRDAASV HGESGMAGYD FVEHNRKPLG IPAFLAIRDA LMRAPEPVTL 130 140 150 160 170 180 VAIGPLTNIA LLLSQCPECK PYIRRLVIMG GSAGRGNCTP NAEFNIAADP EAAACVFRSG 190 200 210 220 230 240 IEIVMCGLDV TNQAILTPDY LSTLPQLNRT GKMLHALFSH YRSGSMQSGL RMHDLCAIAW 250 260 270 280 290 300 LVRPDLFTLK PCFVAVETQG EFTSGTTVVD IDGCLGKPAN VQVALDLDVK GFQQWVAEVL ALAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the lsp-dapB interval in Escherichia coli." Bouvier J., Stragier P. Nucleic Acids Res. 19:180-180(1991) [PubMed: 2011499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The RihA, RihB, and RihC ribonucleoside hydrolases of Escherichia coli. Substrate specificity, gene expression, and regulation." Petersen C., Moeller L.B. J. Biol. Chem. 276:884-894(2001) [PubMed: 11027694] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54945 Genomic DNA. Translation: CAA38708.1. U00096 Genomic DNA. Translation: AAC73141.1. AP009048 Genomic DNA. Translation: BAB96599.1.
PIR	JE0404.
RefSeq	AP_000694.1. NP_414571.1.
3D structure databases
ProteinModelPortal	P22564.
SMR	P22564. Positions 2-300.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11157N.
MINT	MINT-1268238.
STRING	P22564.
Genome annotation databases
EnsemblBacteria	EBESCT00000002953; EBESCP00000002953; EBESCG00000002418. EBESCT00000015519; EBESCP00000014810; EBESCG00000014579.
GeneID	944796.
GenomeReviews	Gene locus JW0028 in contig AP009048_GR. Gene locus b0030 in contig U00096_GR.
KEGG	ecj:JW0028. eco:b0030.
Organism-specific databases
EchoBASE	EB1074.
EcoGene	EG11082. rihC.
CMR	Search...
Phylogenomic databases
eggNOG	COG1957.
HOGENOM	HBG714567.
OMA	EVFACAP.
ProtClustDB	PRK10768.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11082-MONOMER. MetaCyc:EG11082-MONOMER.
Gene expression databases
Genevestigator	P22564.
Family and domain databases
HAMAP	MF_01432. Nucleosid_hydro_RihC. [Tree]
InterPro	IPR015910. I/U_nuclsd_hydro_CS. IPR001910. Inosine/uridine_Nucl_hydrolase. [Graphical view]
Gene3D	G3DSA:3.90.245.10. I/U_nuclsd_hydro. 1 hit.
PANTHER	PTHR12304. I/U_nuclsd_hydro. 1 hit.
Pfam	PF01156. IU_nuc_hydro. 1 hit. [Graphical view]
SUPFAM	SSF53590. I/U_nuclsd_hydro. 1 hit.
PROSITE	PS01247. IUNH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIHC_ECOLI

Accession

Primary (citable) accession number: P22564

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	August 10, 2010
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)