P22541 (GUNA_BUTFI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase A
EC=3.2.1.4

Alternative name(s):
Cellulase A
Endo-1,4-beta-glucanase A
Short name=EgA

Gene names

Name:

celA

Organism

Butyrivibrio fibrisolvens

Taxonomic identifier

831 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Lachnospiraceae › Butyrivibrio

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Ref.1
	Chain	35 – 429	395	Endoglucanase A		PRO_0000007842
Sites
	Active site	249	1	Proton donor By similarity
	Active site	334	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P22541 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 7144A574505E2EAE	FASTA	429	48,859
	10 20 30 40 50 60 MVSKKQKFLT VILVIVLAIV IVGGVFGISF VKGRVTFPWQ LQNSEAKTEQ VKEPAKEEPK 70 80 90 100 110 120 LVIKEKKQDE SAKKEQELKK AKEEAEAAVE KETEKTEEEP VDNLLNDMKL KYYGKLAVEG 130 140 150 160 170 180 SHLVDADGHE VLLMGVSTHG INWYPEYASA ETIKSLRDTW GINVIRLAMY TSDYNGYCVA 190 200 210 220 230 240 GKENQEKLKD IIDDAVEAAT DNDMYVIIDW HTLNDADPNE YKADAIQFFG EMVRKYKDNE 250 260 270 280 290 300 NVIYEICNEP NGDTTWNDVR RYANEVIPVI RNVDAIILVG TPKWATDLDS VLDKPLDFDN 310 320 330 340 350 360 IMYTYHFYAG THHKAERNAL RDALDEGLPV FISEYGLVDA DGDGNLNEKE ADYWYDMIRK 370 380 390 400 410 420 EYGVSSCMWN LSNKDEGSAM INADCDKLSD FTEEDLSESA MWLIDQISQL KHSDLEQGVD WITPENNNR

References

[1]

"Cloning and sequencing of the celA gene encoding endoglucanase A of Butyrivibrio fibrisolvens strain A46." Hazlewood G.P., Davidson K., Laurie J.I., Romaniec M.P.M., Gilbert H.J. J. Gen. Microbiol. 136:2089-2097(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 35-50. Strain: A46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M37031 Genomic DNA. Translation: AAA20893.1.
PIR	S29044.
3D structure databases
ProteinModelPortal	P22541.
ModBase	Search...
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNA_BUTFI

Accession

Primary (citable) accession number: P22541

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program