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Protein

Endoglucanase A

Gene

celA

Organism
Caldicellulosiruptor saccharolyticus (Caldocellum saccharolyticum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The N-terminal domain of CelA codes for an endoglucanase activity on carboxymethylcellulose. The C-terminal domain probably acts synergistically to hydrolyze crystalline cellulose.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei396By similarity1
Active sitei434By similarity1
Active sitei443By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM3 Carbohydrate-Binding Module Family 3
GH48 Glycoside Hydrolase Family 48
GH9 Glycoside Hydrolase Family 9

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase A (EC:3.2.1.4)
Alternative name(s):
Cellulase A
Endo-1,4-beta-glucanase A
Gene namesi
Name:celA
OrganismiCaldicellulosiruptor saccharolyticus (Caldocellum saccharolyticum)
Taxonomic identifieri44001 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisCaldicellulosiruptor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000000794424 – 1742Endoglucanase AAdd BLAST1719

Post-translational modificationi

The linker region (also termed "hinge") may be a potential site for proteolysis.

Structurei

3D structure databases

ProteinModelPortaliP22534
SMRiP22534
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini477 – 637CBM3 1PROSITE-ProRule annotationAdd BLAST161
Domaini703 – 856CBM3 2PROSITE-ProRule annotationAdd BLAST154
Domaini906 – 1059CBM3 3PROSITE-ProRule annotationAdd BLAST154

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 476Catalytic 1Add BLAST453
Regioni638 – 702Linker ("hinge") (Pro-Thr box)Add BLAST65
Regioni857 – 905Linker ("hinge") (Pro-Thr box)Add BLAST49
Regioni1060 – 1112Linker ("hinge") (Pro-Thr box)Add BLAST53
Regioni1113 – 1742Catalytic 2Add BLAST630

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyl hydrolase 9 (cellulase E) family.Curated
In the C-terminal section; belongs to the glycosyl hydrolase 48 (cellulase L) family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4105CYP Bacteria
COG2730 LUCA

Family and domain databases

Gene3Di1.50.10.10, 6 hits
2.170.160.10, 3 hits
2.60.40.710, 3 hits
4.10.870.10, 1 hit
InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR008965 CBM2/CBM3_carb-bd_dom_sf
IPR001956 CBM3
IPR036966 CBM3_sf
IPR023309 Endo-1-4-beta-glucanase_dom2
IPR027390 Endoglucanase_F_dom3
IPR000556 Glyco_hydro_48F
IPR001701 Glyco_hydro_9
IPR033126 Glyco_hydro_9_Asp/Glu_AS
IPR018221 Glyco_hydro_9_His_AS
PfamiView protein in Pfam
PF00942 CBM_3, 3 hits
PF02011 Glyco_hydro_48, 1 hit
PF00759 Glyco_hydro_9, 1 hit
PRINTSiPR00844 GLHYDRLASE48
SMARTiView protein in SMART
SM01067 CBM_3, 3 hits
SUPFAMiSSF48208 SSF48208, 2 hits
SSF49384 SSF49384, 3 hits
PROSITEiView protein in PROSITE
PS51172 CBM3, 3 hits
PS00592 GLYCOSYL_HYDROL_F9_1, 1 hit
PS00698 GLYCOSYL_HYDROL_F9_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22534-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVTFLFILG VVYGVKPWQE ARAGSFNYGE ALQKAIMFYE FQMSGKLPNW
60 70 80 90 100
VRNNWRGDSA LKDGQDNGLD LTGGWFDAGD HVKFNLPMSY TGTMLSWAAY
110 120 130 140 150
EYKDAFVKSG QLEHILNQIE WVNDYFVKCH PSKYVYYYQV GDGGKDHAWW
160 170 180 190 200
GPAEVMQMER PSFKVTQSSP GSAVVAETAA SLAAASIVLK DRNPTKAATY
210 220 230 240 250
LQHAKDLYEF AEVTKSDSGY TAANGYYNSW SGFYDELSWA AVWLYLATND
260 270 280 290 300
STYLTKAESY VQNWPKISGS NIIDYKWAHC WDDVHNGAAL LLAKITDKDT
310 320 330 340 350
YKQIIESHLD YWTTGYNGER IKYTPKGLAW LDQWGSLRYA TTTAFLAFVY
360 370 380 390 400
SDWSGCPTGK KETYRKFGES QIDYALGSTG RSFVVGFGTN PPKRPHHRTA
410 420 430 440 450
HSSWADSQSI PSYHRHTLYG ALVGGPGSDD SYTDDISNYV NNEVACDYNA
460 470 480 490 500
GFVGALAKMY LLYGGNPIPD FKAIETPTND EFFVEAGINA SGTNFIEIKA
510 520 530 540 550
IVNNQSGWPA RATNKLKFRY FVDLSELIKA GYSPNQLTLS TNYNQGAKVS
560 570 580 590 600
GPYVWDSSRN IYYILVDFTG TLIYPGGQDK YKKEVQFRIA APQNVQWDNS
610 620 630 640 650
NDYSFQDIKG VSSGSVVKTK YIPLYDEDIK VWGEEPGTSG VSPTPTASVT
660 670 680 690 700
PTPTPTPTAT PTPTPTPTVT PTPTVTATPT PTPTPTSTPT VTPTPTPVST
710 720 730 740 750
PATSGQIKVL YANKETNSTT NTIRPWLKVV NSGSSSIDLS RVTIRYWYTV
760 770 780 790 800
DGERAQSAIS DWAQIGASNV TFKFVKLSSS VSGADYYLEI GFKSGAGQLQ
810 820 830 840 850
PGKDTGEIQI RFNKDDWSNY NQGNDWSWIQ SMTSYGENEK VTAYIDGVLV
860 870 880 890 900
WGQEPSGTTP APTSTPTVTV TPTPTPTPTV TPTPTVTATP TPTPTPTSTP
910 920 930 940 950
VSTPATGGQI KVLYANKETN STTNTIRPWL KVVNSGSSSI DLSRVTIRYW
960 970 980 990 1000
YTVDGERAQS AISDWAQIGA SNVTFKFVKL SSSVSGADYY LEIGFKSGAG
1010 1020 1030 1040 1050
QLQPGKDTGE IQIRFNKDDW SNYNQGNDWS WIQSMTSYGE NEKVTAYIDG
1060 1070 1080 1090 1100
VLVWGQEPSG ATPAPTVTPT PTVTPTPTPA PTPTATPTPT PTPTVTPTPT
1110 1120 1130 1140 1150
VAPTPTPSST PSGLGKYGQR FMWLWNKIHD PASGYFNQDG IPYHSVETLI
1160 1170 1180 1190 1200
CEAPDYGHLT TSEAFSYYVW LEAVYGKLTG DWSKFKTAWD TLEKYMIPSA
1210 1220 1230 1240 1250
EDQPMRSYDP NKPATYAGEW ETPDKYPSPL EFNVPVGKDP LHNELVSTYG
1260 1270 1280 1290 1300
STLMYGMHWL MDVDNWYGYG KRGDGVSRAS FINTFQRGPE ESVWETVPHP
1310 1320 1330 1340 1350
SWEEFKWGGP NGFLDLFIKD QNYSKQWRYT NAPDADARAI QATYWAKVWA
1360 1370 1380 1390 1400
KEQGKFNEIS SYVGKAAKMG DYLRYAMFDK YFKPLGCQDK NAAGGTGYDS
1410 1420 1430 1440 1450
AHYLLSWYYA WGGALDGAWS WKIGCSHAHF GYQNPMAAWA LANDSDMKPK
1460 1470 1480 1490 1500
SPNGASDWAK SLKRQIEFYR WLQSAEGAIA GGATNSWNGR YEKYPAGTAT
1510 1520 1530 1540 1550
FYGMAYEPNP VYRDPGSNTW FGFQAWSMQR VAEYYYVTGD KDAGTLLEKW
1560 1570 1580 1590 1600
VSWIKSVVKL NSDGTFAIPS TLDWSGQPDT WNGTYTGNPN LHVKVVDYGT
1610 1620 1630 1640 1650
DLGITASLAN ALLYYSAGTK KYGVFDEEAK NLAKELLDRM WKLYRDEKGL
1660 1670 1680 1690 1700
SAPEKRADYK RFFEQEVYIP AGWTGKMPNG DVIKSGVKFI DIRSKYKQDP
1710 1720 1730 1740
DWPKLEAAYK SGQVPEFRYH RFWAQCDIAI VNATYEILFG NQ
Length:1,742
Mass (Da):193,697
Last modified:October 1, 1996 - v2
Checksum:i3F0699A2123EED07
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1545T → A in AAA72860 (PubMed:2039230).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L32742 Genomic DNA Translation: AAA91086.1
M36063 Genomic DNA Translation: AAA72860.1
L01257 Unassigned DNA No translation available.
PIRiT17120

Similar proteinsi

Entry informationi

Entry nameiGUNA_CALSA
AccessioniPrimary (citable) accession number: P22534
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 105 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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