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P22533

- MANB_CALSA

UniProt

P22533 - MANB_CALSA

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Protein

Beta-mannanase/endoglucanase A

Gene
manA
Organism
Caldicellulosiruptor saccharolyticus (Caldocellum saccharolyticum)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Degradation of hemicelluloses, the second most abundant polysaccharides in nature. Contains two catalytic domains with mannanase and endoglucanase activities.

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

pH dependencei

Optimum pH is 6.

Temperature dependencei

Optimum temperature is 80 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei162 – 1621Proton donor By similarity
Active sitei257 – 2571Nucleophile By similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC
  2. cellulose binding Source: InterPro
  3. mannan endo-1,4-beta-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM3. Carbohydrate-Binding Module Family 3.
GH44. Glycoside Hydrolase Family 44.
GH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-mannanase/endoglucanase A
Including the following 2 domains:
Mannan endo-1,4-beta-mannosidase A (EC:3.2.1.78)
Alternative name(s):
Beta-mannanase
Endo-1,4-mannanase
Endo-1,4-beta-glucanase (EC:3.2.1.4)
Alternative name(s):
Cellulase
Gene namesi
Name:manA
OrganismiCaldicellulosiruptor saccharolyticus (Caldocellum saccharolyticum)
Taxonomic identifieri44001 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisCaldicellulosiruptor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4141 Reviewed predictionAdd
BLAST
Chaini42 – 13311290Beta-mannanase/endoglucanase APRO_0000007899Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP22533.
SMRiP22533. Positions 786-1298.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini363 – 516154CBM3 1Add
BLAST
Domaini566 – 719154CBM3 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni42 – 325284Catalytic (mannanase)Add
BLAST
Regioni781 – 1331551Catalytic (endoglucanase)Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi326 – 36136Pro/Ser/Thr-rich (PT box)Add
BLAST
Compositional biasi519 – 56446Pro/Ser/Thr-rich (PT box)Add
BLAST
Compositional biasi721 – 78060Pro/Ser/Thr-rich (PT box)Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyl hydrolase 5 (cellulase A) family.
In the C-terminal section; belongs to the glycosyl hydrolase 44 (cellulase J) family.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG2730.

Family and domain databases

Gene3Di2.60.40.710. 2 hits.
3.20.20.80. 3 hits.
InterProiIPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR024745. Glyco_hydro_44.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00942. CBM_3. 2 hits.
PF00150. Cellulase. 1 hit.
PF12891. Glyco_hydro_44. 1 hit.
[Graphical view]
SMARTiSM01067. CBM_3. 2 hits.
[Graphical view]
SUPFAMiSSF49384. SSF49384. 2 hits.
SSF51445. SSF51445. 3 hits.
PROSITEiPS51172. CBM3. 2 hits.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22533-1 [UniParc]FASTAAdd to Basket

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MRLKTKIRKK WLSVLCTVVF LLNILFIANV TILPKVGAAT SNDGVVKIDT     50
STLIGTNHAH CWYRDRLDTA LRGIRSWGMN SVRVVLSNGY RWTKIPASEV 100
ANIISLSRSL GFKAIILEVH DTTGYGEDGA ACSLAQAVEY WKEIKSVLDG 150
NEDFVIINIG NEPYGNNNYQ NWVNDTKNAI KALRDAGFKH TIMVDAPNWG 200
QDWSNTMRDN AQSIMEADPL RNLVFSIHMY GVYNTASKVE EYIKSFVDKG 250
LPLVIGEFGH QHTDGDPDEE AIVRYAKQYK IGLFSWSWCG NSSYVGYLDM 300
VNNWDPNNPT PWGQWYKTNA IGTSSTPTPT STVTPTPTPT PTPTPTVTAT 350
PTPTPTPVST PATSGQIKVL YANKETNSTT NTIRPWLKVV NSGSSSIDLS 400
RVTIRYWYTV DGERAQSAIS DWAQIGASNV TFKFVKLSSS VSGADYYLEI 450
GFKSGAGQLQ PGKDTGEIQM RFNKDDWSNY NQGNDWSWIQ SMTSYGENEK 500
VTAYIDGVLV WGQEPSGATP APAPTATPTP TPTVTPTPTV TPTPTVTATP 550
TPTPTPTPTP VSTPATGGQI KVLYANKETN STTNTIRPWL KVVNSGSSSI 600
DLSRVTIRYW YTVDGERAQS AISDWAQIGA SNVTFKFVKL SSSVSGADYY 650
LEIGFKSGAG QLQPGKDTGE IQIRFNKSDW SNYNQGNDWS WIQSMTSYGE 700
NEKVTAYIDG VLVWGQEPSG TTPSPTSTPT VTVTPTPTPT PTPTPTPTVT 750
PTPTVTPTPT VTATPTPTPT PIPTVTPLPT ISPSPSVVEI TINTNAGRTQ 800
ISPYIYGANQ DIEGVVHSAR RLGGNRLTGY NWENNFSNAG NDWYHSSDDY 850
LCWSMGISGE DAKVPAAVVS KFHEYSLKNN AYSAVTLQMA GYVSKDNYGT 900
VSENETAPSN RWAEVKFKKD APLSLNPDLN DNFVYMDEFI NYLINKYGMA 950
SSPTGIKGYI LDNEPDLWAS THPRIHPNKV TCKELIEKSV ELAKVIKTLD 1000
PSAEVFGYAS YGFMGYYSLQ DAPDWNQVKG EHRWFISWYL EQMKKASDSF 1050
GKRLLDVLDL HWYPEARGGN IRVCFDGEND TSKEVVIARM QAPRTLWDPT 1100
YKTSVKGQIT AGENSWINQW FSDYLPIIPN VKADIEKYYP GTKLAISEFD 1150
YGGRNHISGG IALADVLGIF GKYGVNFAAR WGDSGSYAAA AYNIYLNYDG 1200
KGSKYGNTNV SANTSDVENM PVYASINGQD DSELHIILIN RNYDQKLQVK 1250
INITSTPKYT KAEIYGFDSN SPEYKKMGNI DNIESNVFTL EVPKFNGVSH 1300
SITLDFNVSI KIIQNEVIKF IRNLVFMRAL V 1331
Length:1,331
Mass (Da):146,893
Last modified:July 1, 1993 - v2
Checksum:iFFBCA51BB8D8F0E0
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti338 – 3381T → P1 Publication
Sequence conflicti340 – 3467TPTPTPT → RQHQHRQ1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L01257 Unassigned DNA. Translation: AAA71887.1.
M36063 Genomic DNA. Translation: AAA72861.1.
PIRiA48954.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L01257 Unassigned DNA. Translation: AAA71887.1 .
M36063 Genomic DNA. Translation: AAA72861.1 .
PIRi A48954.

3D structure databases

ProteinModelPortali P22533.
SMRi P22533. Positions 786-1298.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM3. Carbohydrate-Binding Module Family 3.
GH44. Glycoside Hydrolase Family 44.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG2730.

Family and domain databases

Gene3Di 2.60.40.710. 2 hits.
3.20.20.80. 3 hits.
InterProi IPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR024745. Glyco_hydro_44.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00942. CBM_3. 2 hits.
PF00150. Cellulase. 1 hit.
PF12891. Glyco_hydro_44. 1 hit.
[Graphical view ]
SMARTi SM01067. CBM_3. 2 hits.
[Graphical view ]
SUPFAMi SSF49384. SSF49384. 2 hits.
SSF51445. SSF51445. 3 hits.
PROSITEi PS51172. CBM3. 2 hits.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The beta-mannanase from 'Caldocellum saccharolyticum' is part of a multidomain enzyme."
    Gibbs M.D., Saul D.J., Luthi E., Bergquist P.L.
    Appl. Environ. Microbiol. 58:3864-3867(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning, sequence analysis, and expression in Escherichia coli of a gene coding for a beta-mannanase from the extremely thermophilic bacterium 'Caldocellum saccharolyticum'."
    Luethi E., Jasmat N.B., Grayling R.A., Love D.R., Bergquist P.L.
    Appl. Environ. Microbiol. 57:694-700(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-346.

Entry informationi

Entry nameiMANB_CALSA
AccessioniPrimary (citable) accession number: P22533
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: July 1, 1993
Last modified: December 11, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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