Chromosome partition protein MukE - Escherichia coli (strain K12)

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P22524 (MUKE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 109. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Chromosome partition protein MukE

Alternative name(s):
Protein KicA

Gene names

Name:	mukE
Synonyms:	kicA, ycbA
Ordered Locus Names:	b0923, JW0906

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in chromosome condensation, segregation and cell cycle progression. May participate in facilitating chromosome segregation by condensation DNA from both sides of a centrally located replisome during cell division. Probably acts via its interaction with MukB and MukF. Ref.7
Subunit structure	Forms a hexameric complex with MukF, (MukF-(MukE)2)2, which forms a ternary complex with MukB. The complex formation is stimulated by calcium or magnesium. Ref.9
Subcellular location	Cytoplasm › nucleoid. Note: Restricted to the nucleoid region. HAMAP-Rule MF_01802
Sequence similarities	Belongs to the MukE family.
Sequence caution	The sequence BAA05458.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAA35669.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAA40775.1 differs from that shown. Reason: Frameshift at position 105.

Ontologies

Keywords
Biological process	Cell cycle Cell division Chromosome partition DNA condensation
Cellular component	Cytoplasm
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome condensation Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: HAMAP nucleoid Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
mukF	P60293	4	EBI-554672,EBI-554679

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 234

234

Chromosome partition protein MukE HAMAP-Rule MF_01802

PRO_0000206792

Secondary structure

234

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P22524 [UniParc].

Last modified November 24, 2009. Version 5.
Checksum: 1E771144C58207DD
Show »

FASTA

234

26,974

        10         20         30         40         50         60 
MSSTNIEQVM PVKLAQALAN PLFPALDSAL RSGRHIGLDE LDNHAFLMDF QEYLEEFYAR 

        70         80         90        100        110        120 
YNVELIRAPE GFFYLRPRST TLIPRSVLSE LDMMVGKILC YLYLSPERLA NEGIFTQQEL 

       130        140        150        160        170        180 
YDELLTLADE AKLLKLVNNR STGSDVDRQK LQEKVRSSLN RLRRLGMVWF MGHDSSKFRI 

       190        200        210        220        230 
TESVFRFGAD VRAGDDPREA QRRLIRDGEA MPIENHLQLN DETEENQPDS GEEE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"New killing system controlled by two genes located immediately upstream of the mukB gene in Escherichia coli." Feng J., Yamanaka K., Niki H., Ogura T., Hiraga S. Mol. Gen. Genet. 243:136-147(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Escherichia coli condensin MukB stimulates topoisomerase IV activity by a direct physical interaction." Li Y., Stewart N.K., Berger A.J., Vos S., Schoeffler A.J., Berger J.M., Chait B.T., Oakley M.G. Proc. Natl. Acad. Sci. U.S.A. 107:18832-18837(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 14-31; 68-78 AND 109-132, INTERACTION WITH MUKB.
[6]	"The new gene mukB codes for a 177 kd protein with coiled-coil domains involved in chromosome partitioning of E. coli." Niki H., Jaffe A., Imamura R., Ogura T., Hiraga S. EMBO J. 10:183-193(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 83-234.
[7]	"Identification of two new genes, mukE and mukF, involved in chromosome partitioning in Escherichia coli." Yamanaka K., Ogura T., Niki H., Hiraga S. Mol. Gen. Genet. 250:241-251(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Complex formation of MukB, MukE and MukF proteins involved in chromosome partitioning in Escherichia coli." Yamazoe M., Onogi T., Sunako Y., Niki H., Yamanaka K., Ichimura T., Hiraga S. EMBO J. 18:5873-5884(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, INTERACTION WITH MUKB AND MUKF.
[9]	"Structural studies of a bacterial condensin complex reveal ATP-dependent disruption of intersubunit interactions." Woo J.-S., Lim J.-H., Shin H.-C., Suh M.-K., Ku B., Lee K.-H., Joo K., Robinson H., Lee J., Park S.-Y., Ha N.-C., Oh B.-H. Cell 136:85-96(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS), SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D26440 Genomic DNA. Translation: BAA05458.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74009.2.
AP009048 Genomic DNA. Translation: BAA35669.2. Different initiation.
X57550 Genomic DNA. Translation: CAA40775.1. Frameshift.

PIR

B64832.

RefSeq

NP_415443.2. NC_000913.2.
YP_489195.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3EUH	X-ray	2.90	C/D/E/F	1-234	[»]
3RPU	X-ray	3.60	D/E/G/H/Y/Z	10-234	[»]

ProteinModelPortal

P22524.

SMR

P22524. Positions 10-215.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-10274N.

IntAct

P22524. 8 interactions.

MINT

MINT-1227862.

STRING

511145.b0923.

Proteomic databases

PaxDb

P22524.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC74009; AAC74009; b0923.
BAA35669; BAA35669; BAA35669.

GeneID

12932875.
945550.

KEGG

ecj:Y75_p0895.
eco:b0923.

PATRIC

32117059. VBIEscCol129921_0954.

Organism-specific databases

EchoBASE

EB1232.

EcoGene

EG11252. mukE.

Phylogenomic databases

eggNOG

COG3095.

HOGENOM

HOG000278254.

K03804.

ProtClustDB

PRK05256.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11252-MONOMER.
ECOL316407:JW0906-MONOMER.

Gene expression databases

Genevestigator

P22524.

Family and domain databases

HAMAP

MF_01802. MukE.

InterPro

IPR007385. Scp_MukE.
[Graphical view]

Pfam

PF04288. MukE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P22524.

Entry information

Entry name

MUKE_ECOLI

Accession

Primary (citable) accession number: P22524

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	November 24, 2009
Last modified:	May 1, 2013
This is version 109 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents