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Protein

Chromosome partition protein MukB

Gene

mukB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a central role in chromosome condensation, segregation and cell cycle progression. Functions as a homodimer, which is essential for chromosome partition. Involved in negative DNA supercoiling in vivo, and by this means organizes and compacts chromosomes. May achieve or facilitate chromosome segregation by condensation of DNA from both sides of a centrally located replisome during cell division. Stimulates both DNA relaxation and to a lesser extent decatenation activity of topoisomerase IV.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi34 – 41ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: EcoCyc
  • DNA binding Source: EcoCyc
  • GTP binding Source: EcoCyc
  • identical protein binding Source: EcoCyc

GO - Biological processi

  • cell division Source: EcoCyc
  • chromosome condensation Source: EcoCyc
  • chromosome segregation Source: EcoliWiki
  • sister chromatid cohesion Source: EcoliWiki

Keywordsi

Molecular functionDNA-binding
Biological processCell cycle, Cell division, Chromosome partition, DNA condensation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10618-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosome partition protein MukB
Alternative name(s):
Structural maintenance of chromosome-related protein
Gene namesi
Name:mukB
Ordered Locus Names:b0924, JW0907
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10618 mukB

Subcellular locationi

GO - Cellular componenti

  • condensin complex Source: EcoCyc
  • cytosol Source: EcoCyc
  • nucleoid Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi33S → F in mukB106; no effect. 1 Publication1
Mutagenesisi40K → L: No effect. 1 Publication1
Mutagenesisi688E → A: Does not rescue a ts-mutant of MukB. 1 Publication1
Mutagenesisi692D → A: Does not rescue a ts-mutant of MukB; less binding of ParC. 1 Publication1
Mutagenesisi1201D → N in mukB33; no effect. 1 Publication1
Mutagenesisi1381V → L: Abolishes DNA-binding, but remains associated with MukE and MukF. 1 Publication1
Mutagenesisi1403L → P: Abolishes association with MukE and MukF, but still binds DNA. 1 Publication1
Mutagenesisi1404F → L: No effect. 1 Publication1
Mutagenesisi1406D → N: No effect. 1 Publication1
Mutagenesisi1428M → L: No effect. 1 Publication1
Mutagenesisi1429Q → R: Abolishes association with MukE and MukF, but still binds DNA. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000682141 – 1486Chromosome partition protein MukBAdd BLAST1486

Proteomic databases

EPDiP22523
PaxDbiP22523
PRIDEiP22523

Interactioni

Subunit structurei

Homodimerization via its hinge domain. Binds to DNA via its C-terminal region. Interacts, and probably forms a ternary complex, with MukE and MukF via its C-terminal region. The complex formation is stimulated by calcium or magnesium. Interacts with tubulin-related protein FtsZ. Identified in a complex with SpoT; IscS and ACP. Interacts with the ParC subunit of topoisomerase IV.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
parCP0AFI211EBI-542943,EBI-878544

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4261687, 403 interactors
ComplexPortaliCPX-1090 MukBEF condensin complex
DIPiDIP-10273N
IntActiP22523, 58 interactors
MINTiP22523
STRINGi316385.ECDH10B_0994

Structurei

Secondary structure

11486
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 15Combined sources10
Beta strandi18 – 23Combined sources6
Helixi25 – 33Combined sources9
Helixi38 – 51Combined sources14
Turni55 – 57Combined sources3
Helixi78 – 80Combined sources3
Beta strandi83 – 93Combined sources11
Beta strandi99 – 108Combined sources10
Turni112 – 114Combined sources3
Beta strandi116 – 125Combined sources10
Helixi132 – 136Combined sources5
Beta strandi141 – 143Combined sources3
Helixi150 – 158Combined sources9
Beta strandi164 – 167Combined sources4
Helixi171 – 180Combined sources10
Beta strandi183 – 186Combined sources4
Helixi191 – 205Combined sources15
Beta strandi206 – 208Combined sources3
Helixi210 – 220Combined sources11
Helixi573 – 617Combined sources45
Helixi645 – 663Combined sources19
Helixi674 – 681Combined sources8
Helixi686 – 689Combined sources4
Helixi690 – 692Combined sources3
Turni695 – 697Combined sources3
Helixi698 – 704Combined sources7
Helixi706 – 710Combined sources5
Beta strandi712 – 715Combined sources4
Helixi717 – 719Combined sources3
Helixi721 – 724Combined sources4
Beta strandi732 – 739Combined sources8
Beta strandi743 – 745Combined sources3
Beta strandi751 – 754Combined sources4
Beta strandi757 – 763Combined sources7
Beta strandi766 – 771Combined sources6
Helixi780 – 802Combined sources23
Turni825 – 828Combined sources4
Beta strandi829 – 831Combined sources3
Helixi834 – 853Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QHLX-ray2.20A1-227[»]
2WMMX-ray2.30A/B645-804[»]
3IBPX-ray3.10A566-863[»]
4MN4X-ray2.30C/D645-804[»]
ProteinModelPortaliP22523
SMRiP22523
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22523

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni645 – 804Sufficient for ParC bindingAdd BLAST160
Regioni666 – 783Flexible hingeAdd BLAST118

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili326 – 418Sequence analysisAdd BLAST93
Coiled coili444 – 480Sequence analysisAdd BLAST37
Coiled coili509 – 603Sequence analysisAdd BLAST95
Coiled coili835 – 923Sequence analysisAdd BLAST89
Coiled coili977 – 1115Sequence analysisAdd BLAST139
Coiled coili1209 – 1266Sequence analysisAdd BLAST58

Domaini

The hinge domain, which separates the large intramolecular coiled coil regions, allows the homodimerization, forming a V-shaped homodimer. The N- and C-terminus together form the head domain.

Sequence similaritiesi

Belongs to the SMC family. MukB subfamily.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4105DUC Bacteria
COG3096 LUCA
HOGENOMiHOG000278243
InParanoidiP22523
KOiK03632
OMAiLEDCPED
PhylomeDBiP22523

Family and domain databases

HAMAPiMF_01800 MukB, 1 hit
InterProiView protein in InterPro
IPR012090 MukB
IPR032520 MukB_hinge
IPR007406 MukB_N_dom
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF04310 MukB, 1 hit
PF16330 MukB_hinge, 1 hit
PIRSFiPIRSF005246 MukB, 1 hit
SUPFAMiSSF52540 SSF52540, 3 hits

Sequencei

Sequence statusi: Complete.

P22523-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIERGKFRSL TLINWNGFFA RTFDLDELVT TLSGGNGAGK STTMAAFVTA
60 70 80 90 100
LIPDLTLLHF RNTTEAGATS GSRDKGLHGK LKAGVCYSML DTINSRHQRV
110 120 130 140 150
VVGVRLQQVA GRDRKVDIKP FAIQGLPMSV QPTQLVTETL NERQARVLPL
160 170 180 190 200
NELKDKLEAM EGVQFKQFNS ITDYHSLMFD LGIIARRLRS ASDRSKFYRL
210 220 230 240 250
IEASLYGGIS SAITRSLRDY LLPENSGVRK AFQDMEAALR ENRMTLEAIR
260 270 280 290 300
VTQSDRDLFK HLISEATNYV AADYMRHANE RRVHLDKALE FRRELHTSRQ
310 320 330 340 350
QLAAEQYKHV DMARELAEHN GAEGDLEADY QAASDHLNLV QTALRQQEKI
360 370 380 390 400
ERYEADLDEL QIRLEEQNEV VAEAIERQQE NEARAEAAEL EVDELKSQLA
410 420 430 440 450
DYQQALDVQQ TRAIQYNQAI AALNRAKELC HLPDLTADCA AEWLETFQAK
460 470 480 490 500
ELEATEKMLS LEQKMSMAQT AHSQFEQAYQ LVVAINGPLA RNEAWDVARE
510 520 530 540 550
LLREGVDQRH LAEQVQPLRM RLSELEQRLR EQQEAERLLA DFCKRQGKNF
560 570 580 590 600
DIDELEALHQ ELEARIASLS DSVSNAREER MALRQEQEQL QSRIQSLMQR
610 620 630 640 650
APVWLAAQNS LNQLSEQCGE EFTSSQDVTE YLQQLLERER EAIVERDEVG
660 670 680 690 700
ARKNAVDEEI ERLSQPGGSE DQRLNALAER FGGVLLSEIY DDVSLEDAPY
710 720 730 740 750
FSALYGPSRH AIVVPDLSQV TEHLEGLTDC PEDLYLIEGD PQSFDDSVFS
760 770 780 790 800
VDELEKAVVV KIADRQWRYS RFPEVPLFGR AARESRIESL HAEREVLSER
810 820 830 840 850
FATLSFDVQK TQRLHQAFSR FIGSHLAVAF ESDPEAEIRQ LNSRRVELER
860 870 880 890 900
ALSNHENDNQ QQRIQFEQAK EGVTALNRIL PRLNLLADDS LADRVDEIRE
910 920 930 940 950
RLDEAQEAAR FVQQFGNQLA KLEPIVSVLQ SDPEQFEQLK EDYAYSQQMQ
960 970 980 990 1000
RDARQQAFAL TEVVQRRAHF SYSDSAEMLS GNSDLNEKLR ERLEQAEAER
1010 1020 1030 1040 1050
TRAREALRGH AAQLSQYNQV LASLKSSYDT KKELLNDLQR ELQDIGVRAD
1060 1070 1080 1090 1100
SGAEERARIR RDELHAQLSN NRSRRNQLEK ALTFCEAEMD NLTRKLRKLE
1110 1120 1130 1140 1150
RDYFEMREQV VTAKAGWCAV MRMVKDNGVE RRLHRRELAY LSADDLRSMS
1160 1170 1180 1190 1200
DKALGALRLA VADNEHLRDV LRMSEDPKRP ERKIQFFVAV YQHLRERIRQ
1210 1220 1230 1240 1250
DIIRTDDPVE AIEQMEIELS RLTEELTSRE QKLAISSRSV ANIIRKTIQR
1260 1270 1280 1290 1300
EQNRIRMLNQ GLQNVSFGQV NSVRLNVNVR ETHAMLLDVL SEQHEQHQDL
1310 1320 1330 1340 1350
FNSNRLTFSE ALAKLYQRLN PQIDMGQRTP QTIGEELLDY RNYLEMEVEV
1360 1370 1380 1390 1400
NRGSDGWLRA ESGALSTGEA IGTGMSILVM VVQSWEDESR RLRGKDISPC
1410 1420 1430 1440 1450
RLLFLDEAAR LDARSIATLF ELCERLQMQL IIAAPENISP EKGTTYKLVR
1460 1470 1480
KVFQNTEHVH VVGLRGFAPQ LPETLPGTDE APSQAS
Length:1,486
Mass (Da):170,230
Last modified:July 15, 1998 - v2
Checksum:i38C7874BEB78D6D6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti266A → R in CAA40776 (PubMed:1989883).Curated1
Sequence conflicti266A → R in BAA06510 (PubMed:7988894).Curated1
Sequence conflicti318 – 319EH → DD in CAA40776 (PubMed:1989883).Curated2
Sequence conflicti1134H → D in CAA40776 (PubMed:1989883).Curated1
Sequence conflicti1174 – 1175SE → VQ in CAA40776 (PubMed:1989883).Curated2
Sequence conflicti1276 – 1277Missing in CAA40776 (PubMed:1989883).Curated2
Sequence conflicti1276 – 1277Missing in BAA06510 (PubMed:7988894).Curated2
Sequence conflicti1357 – 1380WLRAE…SILVM → CCAQSLVHCRPVRRWYRYVD SGV in CAA40776 (PubMed:1989883).CuratedAdd BLAST24
Sequence conflicti1390 – 1486RRLRG…PSQAS → AACAVKISLLAACCSSMKQR DWMLVLSPRCLNCVSVCKCN SSSQRRKISARRKAPPINWC VKSSRIPNTFMSSACEDLRR NSLKRFQELTKRLLRRVKIK QQCRLFFFRKLRFCTKKVAH YGALFFKLLYIRLCKNVRRL YTEDKPDE in CAA40776 (PubMed:1989883).CuratedAdd BLAST97

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57550 Genomic DNA Translation: CAA40776.1
D31701 Genomic DNA Translation: BAA06510.1
U00096 Genomic DNA Translation: AAC74010.1
AP009048 Genomic DNA Translation: BAA35670.1
D26440 Genomic DNA Translation: BAA05459.1
PIRiC64832
JH0228
RefSeqiNP_415444.1, NC_000913.3
WP_000572698.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74010; AAC74010; b0924
BAA35670; BAA35670; BAA35670
GeneIDi945549
KEGGiecj:JW0907
eco:b0924
PATRICifig|1411691.4.peg.1352

Similar proteinsi

Entry informationi

Entry nameiMUKB_ECOLI
AccessioniPrimary (citable) accession number: P22523
Secondary accession number(s): P71227, P77164, Q47398
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: July 15, 1998
Last modified: June 20, 2018
This is version 174 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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