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Protein

Dual specificity protein kinase CLK1

Gene

Clk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates: SRSF1, SRSF3 and PTPN1. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells and adenovirus E1A pre-mRNA.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Regulates splicing of its own pre-mRNA according to its kinase activity; increased expression of the catalytically active form influences splicing to generate the catalytically inactive splicing variant lacking the kinase domain. Leucettine L41 inhibits its kinase activity and affects the regulation of alternative splicing mediated by phosphorylation of SR proteins.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei190ATPPROSITE-ProRule annotation1
Active sitei287Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi166 – 174ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine/tyrosine kinase activity Source: UniProtKB-EC
  • protein serine/threonine kinase activity Source: MGI
  • protein tyrosine kinase activity Source: MGI

GO - Biological processi

  • peptidyl-serine phosphorylation Source: MGI
  • peptidyl-threonine phosphorylation Source: MGI
  • peptidyl-tyrosine phosphorylation Source: MGI
  • protein autophosphorylation Source: MGI
  • regulation of RNA splicing Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.12.1. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein kinase CLK1 (EC:2.7.12.1)
Alternative name(s):
CDC-like kinase 1
Protein kinase STY
Gene namesi
Name:Clk1
Synonyms:Clk, Sty
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:107403. Clk1.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • cytoplasm Source: Ensembl
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi190K → R: Loss of activity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1075280.
GuidetoPHARMACOLOGYi1990.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000858671 – 483Dual specificity protein kinase CLK1Add BLAST483

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei61PhosphoserineBy similarity1
Modified residuei139PhosphoserineCombined sources1

Post-translational modificationi

Autophosphorylates on all three types of residues.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP22518.
PaxDbiP22518.
PeptideAtlasiP22518.
PRIDEiP22518.

PTM databases

iPTMnetiP22518.
PhosphoSitePlusiP22518.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026034.
CleanExiMM_CLK1.
GenevisibleiP22518. MM.

Interactioni

Subunit structurei

Interacts with PPIG and UBL5.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
lubXQ5ZRQ03EBI-6479117,EBI-6402540From a different organism.

Protein-protein interaction databases

IntActiP22518. 1 interactor.
STRINGi10090.ENSMUSP00000034868.

Chemistry databases

BindingDBiP22518.

Structurei

3D structure databases

ProteinModelPortaliP22518.
SMRiP22518.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini160 – 476Protein kinasePROSITE-ProRule annotationAdd BLAST317

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi29 – 33Nuclear localization signalSequence analysis5

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0671. Eukaryota.
ENOG410XQF2. LUCA.
GeneTreeiENSGT00580000081366.
HOGENOMiHOG000203417.
HOVERGENiHBG107720.
InParanoidiP22518.
KOiK08823.
OMAiYVEEYNP.
OrthoDBiEOG091G033A.
TreeFamiTF101041.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P22518-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRHSKRTYCP DWDERDWDYG TWRSSSSHKR KKRSHSSARE QKRCRYDHSK
60 70 80 90 100
TTDSYYLESR SINEKAYHSR RYVDEYRNDY MGYEPGHPYG EPGSRYQMHS
110 120 130 140 150
SKSSGRSGRS SYKSKHRSRH HTSQHHSHGK SHRRKRSRSV EDDEEGHLIC
160 170 180 190 200
QSGDVLSARY EIVDTLGEGA FGKVVECIDH KVGGRRVAVK IVKNVDRYCE
210 220 230 240 250
AAQSEIQVLE HLNTTDPHST FRCVQMLEWF EHRGHICIVF ELLGLSTYDF
260 270 280 290 300
IKENSFLPFR MDHIRKMAYQ ICKSVNFLHS NKLTHTDLKP ENILFVKSDY
310 320 330 340 350
TEAYNPKMKR DERTIVNPDI KVVDFGSATY DDEHHSTLVS TRHYRAPEVI
360 370 380 390 400
LALGWSQPCD VWSIGCILIE YYLGFTVFPT HDSREHLAMM ERILGPLPKH
410 420 430 440 450
MIQKTRKRRY FHHDRLDWDE HSSAGRYVSR RCKPLKEFML SQDAEHELLF
460 470 480
DLIGKMLEYD PAKRITLKEA LKHPFFYPLK KHT
Length:483
Mass (Da):57,093
Last modified:July 27, 2011 - v2
Checksum:i96FEBC20EC90D8E8
GO
Isoform Short (identifier: P22518-2) [UniParc]FASTAAdd to basket
Also known as: Clk1T

The sequence of this isoform differs from the canonical sequence as follows:
     130-135: KSHRRK → MKLLIL
     136-483: Missing.

Note: Lacks the kinase domain.
Show »
Length:135
Mass (Da):16,318
Checksum:i44BBA47A5D09705A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti379P → S in AAA40151 (PubMed:1986248).Curated1
Sequence conflicti448L → F in CAA40473 (PubMed:1825055).Curated1
Sequence conflicti453I → V in CAA40473 (PubMed:1825055).Curated1
Sequence conflicti456M → I in CAA40473 (PubMed:1825055).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004854130 – 135KSHRRK → MKLLIL in isoform Short. 3 Publications6
Alternative sequenceiVSP_004855136 – 483Missing in isoform Short. 3 PublicationsAdd BLAST348

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57186 mRNA. Translation: CAA40473.1.
M38381 mRNA. Translation: AAA40151.1.
U21209 mRNA. Translation: AAC52257.1.
L29221 mRNA. Translation: AAA61485.1.
AK135765 mRNA. Translation: BAE22647.1.
CH466548 Genomic DNA. Translation: EDL00074.1.
BC145905 mRNA. Translation: AAI45906.1.
BC145911 mRNA. Translation: AAI45912.1.
CCDSiCCDS35577.1. [P22518-1]
PIRiA39676.
I49275.
RefSeqiNP_001036099.1. NM_001042634.2. [P22518-1]
UniGeneiMm.1761.

Genome annotation databases

EnsembliENSMUST00000034868; ENSMUSP00000034868; ENSMUSG00000026034. [P22518-1]
ENSMUST00000148330; ENSMUSP00000137649; ENSMUSG00000026034. [P22518-2]
ENSMUST00000151338; ENSMUSP00000137815; ENSMUSG00000026034. [P22518-2]
GeneIDi12747.
KEGGimmu:12747.
UCSCiuc007bbs.2. mouse. [P22518-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57186 mRNA. Translation: CAA40473.1.
M38381 mRNA. Translation: AAA40151.1.
U21209 mRNA. Translation: AAC52257.1.
L29221 mRNA. Translation: AAA61485.1.
AK135765 mRNA. Translation: BAE22647.1.
CH466548 Genomic DNA. Translation: EDL00074.1.
BC145905 mRNA. Translation: AAI45906.1.
BC145911 mRNA. Translation: AAI45912.1.
CCDSiCCDS35577.1. [P22518-1]
PIRiA39676.
I49275.
RefSeqiNP_001036099.1. NM_001042634.2. [P22518-1]
UniGeneiMm.1761.

3D structure databases

ProteinModelPortaliP22518.
SMRiP22518.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP22518. 1 interactor.
STRINGi10090.ENSMUSP00000034868.

Chemistry databases

BindingDBiP22518.
ChEMBLiCHEMBL1075280.
GuidetoPHARMACOLOGYi1990.

PTM databases

iPTMnetiP22518.
PhosphoSitePlusiP22518.

Proteomic databases

MaxQBiP22518.
PaxDbiP22518.
PeptideAtlasiP22518.
PRIDEiP22518.

Protocols and materials databases

DNASUi12747.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034868; ENSMUSP00000034868; ENSMUSG00000026034. [P22518-1]
ENSMUST00000148330; ENSMUSP00000137649; ENSMUSG00000026034. [P22518-2]
ENSMUST00000151338; ENSMUSP00000137815; ENSMUSG00000026034. [P22518-2]
GeneIDi12747.
KEGGimmu:12747.
UCSCiuc007bbs.2. mouse. [P22518-1]

Organism-specific databases

CTDi1195.
MGIiMGI:107403. Clk1.

Phylogenomic databases

eggNOGiKOG0671. Eukaryota.
ENOG410XQF2. LUCA.
GeneTreeiENSGT00580000081366.
HOGENOMiHOG000203417.
HOVERGENiHBG107720.
InParanoidiP22518.
KOiK08823.
OMAiYVEEYNP.
OrthoDBiEOG091G033A.
TreeFamiTF101041.

Enzyme and pathway databases

BRENDAi2.7.12.1. 3474.

Miscellaneous databases

ChiTaRSiClk1. mouse.
PROiP22518.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026034.
CleanExiMM_CLK1.
GenevisibleiP22518. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLK1_MOUSE
AccessioniPrimary (citable) accession number: P22518
Secondary accession number(s): A6H6K2, Q3UXB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.