Reviewed, UniProtKB/Swiss-Prot P22518 (CLK1_MOUSE)

Names and origin

Protein names

Recommended name:
    Dual specificity protein kinase CLK1
    EC=2.7.12.1
Alternative name(s):
    CDC-like kinase 1
    Protein kinase STY

Gene names

Name:	Clk1
Synonyms:	Clk, Sty

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with PPIG By similarity.
Subcellular location	Nucleus.
Post-translational modification	Autophosphorylates on all three types of residues.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	peptidyl-serine phosphorylation Ref.1 Ref.2 Inferred from direct assay. Source: MGI peptidyl-threonine phosphorylation Ref.2 Inferred from direct assay. Source: MGI peptidyl-tyrosine phosphorylation Ref.1 Ref.2 Inferred from direct assay. Source: MGI protein amino acid autophosphorylation Ref.1 Inferred from direct assay. Source: MGI
Cellular component	nucleus Inferred from direct assay. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Ref.1 Ref.2 Inferred from direct assay. Source: MGI protein tyrosine kinase activity Ref.1 Ref.2 Inferred from direct assay. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: P22518-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P22518-2)

The sequence of this isoform differs from the canonical sequence as follows:
     130-135: KSHRRK → MKLLIL
     136-483: Missing.

Note: Lacks the kinase domain.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 483	483	Dual specificity protein kinase CLK1		PRO_0000085867
Regions
	Domain	160 – 476	317	Protein kinase
	Nucleotide binding	166 – 174	9	ATP By similarity
	Motif	29 – 33	5	Nuclear localization signal Potential
Sites
	Active site	287	1	Proton acceptor By similarity
	Binding site	190	1	ATP By similarity
Amino acid modifications
	Modified residue	37	1	Phosphoserine By similarity
	Modified residue	61	1	Phosphoserine By similarity
	Modified residue	139	1	Phosphoserine By similarity
	Modified residue	340	1	Phosphoserine By similarity
	Modified residue	341	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	130 – 135	6	KSHRRK → MKLLIL in isoform Short.		VSP_004854
	Alternative sequence	136 – 483	348	Missing in isoform Short.		VSP_004855
Experimental info
	Mutagenesis	190	1	K → R: Loss of activity.
	Sequence conflict	379	1	P → S in AAA40151. Ref.2
	Sequence conflict	448	1	F → L in AAA40151. Ref.2
	Sequence conflict	453 – 456	4	VGKI → IGKM Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 1D6EBC2AA2342619	FASTA	483	57,095
	10 20 30 40 50 60 MRHSKRTYCP DWDERDWDYG TWRSSSSHKR KKRSHSSARE QKRCRYDHSK TTDSYYLESR 70 80 90 100 110 120 SINEKAYHSR RYVDEYRNDY MGYEPGHPYG EPGSRYQMHS SKSSGRSGRS SYKSKHRSRH 130 140 150 160 170 180 HTSQHHSHGK SHRRKRSRSV EDDEEGHLIC QSGDVLSARY EIVDTLGEGA FGKVVECIDH 190 200 210 220 230 240 KVGGRRVAVK IVKNVDRYCE AAQSEIQVLE HLNTTDPHST FRCVQMLEWF EHRGHICIVF 250 260 270 280 290 300 ELLGLSTYDF IKENSFLPFR MDHIRKMAYQ ICKSVNFLHS NKLTHTDLKP ENILFVKSDY 310 320 330 340 350 360 TEAYNPKMKR DERTIVNPDI KVVDFGSATY DDEHHSTLVS TRHYRAPEVI LALGWSQPCD 370 380 390 400 410 420 VWSIGCILIE YYLGFTVFPT HDSREHLAMM ERILGPLPKH MIQKTRKRRY FHHDRLDWDE 430 440 450 460 470 480 HSSAGRYVSR RCKPLKEFML SQDAEHEFLF DLVGKILEYD PAKRITLKEA LKHPFFYPLK KHT
	Isoform Short. Checksum: 44BBA47A5D09705A	FASTA	135	16,318
	10 20 30 40 50 60 MRHSKRTYCP DWDERDWDYG TWRSSSSHKR KKRSHSSARE QKRCRYDHSK TTDSYYLESR 70 80 90 100 110 120 SINEKAYHSR RYVDEYRNDY MGYEPGHPYG EPGSRYQMHS SKSSGRSGRS SYKSKHRSRH 130 HTSQHHSHGM KLLIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A mammalian protein kinase with potential for serine/threonine and tyrosine phosphorylation is related to cell cycle regulators." Ben-David Y., Letwin K., Tannock L., Bernstein A., Pawson T. EMBO J. 10:317-325(1991) [PubMed: 1825055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"STY, a tyrosine-phosphorylating enzyme with sequence homology to serine/threonine kinases." Howell B.W., Afar D.E., Lew J., Douville E.M., Icely P.L., Gray D.A., Bell J.C. Mol. Cell. Biol. 11:568-572(1991) [PubMed: 1986248] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Alternative splicing of STY, a nuclear dual specificity kinase." Duncan P.I., Howell B.W., Marius R.M., Drmanic S., Douville E.M., Bell J.C. J. Biol. Chem. 270:21524-21531(1995) [PubMed: 7665564] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[4]	"Characterization by cDNA cloning of two new human protein kinases. Evidence by sequence comparison of a new family of mammalian protein kinases." Hanes J.J., der Kammer H., Klaudiny J.J., Scheit K.H. J. Mol. Biol. 244:665-672(1994) [PubMed: 7990150] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT). Tissue: Brain.
[6]	"The Clk/Sty protein kinase phosphorylates SR splicing factors and regulates their intranuclear distribution." Colwill K., Pawson T., Andrews B., Prasad J., Manley J.L., Bell J.C., Duncan P.I. EMBO J. 15:265-275(1996) [PubMed: 8617202] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X57186 mRNA. Translation: CAA40473.1. M38381 mRNA. Translation: AAA40151.1. U21209 mRNA. Translation: AAC52257.1. L29221 mRNA. Translation: AAA61485.1. BC145905 mRNA. Translation: AAI45906.1. BC145911 mRNA. Translation: AAI45912.1.
IPI	IPI00129398. IPI00230188.
PIR	A39676. I49275.
UniGene	Mm.1761
3D structure databases
HSSP	HSSP built from PDB template 1BI8 based on UniProtKB Q00534.
SMR	P22518. Positions 147-481.
ModBase	Search...
Protein-protein interaction databases
STRING	P22518.
PTM databases
PhosphoSite	P22518.
Proteomic databases
PRIDE	P22518.
Genome annotation databases
Ensembl	ENSMUST00000027195; ENSMUSP00000027195; ENSMUSG00000026034; Mus musculus. [Genome view] ENSMUST00000034868; ENSMUSP00000034868; ENSMUSG00000026034; Mus musculus. [Genome view]
UCSC	uc007bbt.1. mouse.
Organism-specific databases
MGI	MGI:107403. Clk1.
Phylogenomic databases
HOGENOM	P22518.
HOVERGEN	P22518.
Enzyme and pathway databases
BRENDA	2.7.12.1. 244.
Gene expression databases
ArrayExpress	P22518.
Bgee	P22518.
CleanEx	MM_CLK1.
Genevestigator	P22518.
GermOnline	ENSMUSG00000026034. Mus musculus.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

CLK1_MOUSE

Accession

Primary (citable) accession number: P22518
Secondary accession number(s): A6H6K2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	October 13, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)