Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P22517 (KCC2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calcium/calmodulin-dependent protein kinase II

EC=2.7.11.17
Gene names
Name:CMK2
Ordered Locus Names:YOL016C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length447 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Important in cellular regulation.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Multimeric.

Miscellaneous

Present with 7500 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 447447Calcium/calmodulin-dependent protein kinase II
PRO_0000086105

Regions

Domain47 – 309263Protein kinase
Nucleotide binding53 – 619ATP By similarity
Region323 – 33412Calmodulin-binding By similarity

Sites

Active site1711Proton acceptor By similarity
Binding site761ATP By similarity

Amino acid modifications

Modified residue3161Phosphothreonine; by autocatalysis By similarity
Modified residue3531Phosphotyrosine Ref.9
Modified residue3541Phosphoserine Ref.9
Modified residue3671Phosphoserine Ref.6 Ref.9
Modified residue3711Phosphoserine Ref.9
Modified residue3871Phosphoserine; by autocatalysis By similarity
Modified residue4431Phosphoserine Ref.7

Experimental info

Sequence conflict2821V → A in CAA40281. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P22517 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 5990740B980B8312

FASTA44750,447
        10         20         30         40         50         60 
MPKESEVINS EFHVDVQDPE RLNGHPVAKF INKLSGQPES YVNRTNYIFG RTLGAGSFGV 

        70         80         90        100        110        120 
VRQARKLSTN EDVAIKILLK KALQGNNVQL QMLYEELSIL QKLSHPNIVS FKDWFESKDK 

       130        140        150        160        170        180 
FYIVTQLATG GELFDRILSR GKFTEVDAVE IIVQILGAVE YMHSKNVVHR DLKPENVLYV 

       190        200        210        220        230        240 
DKSENSPLVI ADFGIAKQLK GEEDLIYKAA GSLGYVAPEV LTQDGHGKPC DIWSIGVITY 

       250        260        270        280        290        300 
TLLCGYSPFI AESVEGFMEE CTASRYPVTF HMPYWDNISI DVKRFILKAL RLNPADRPTA 

       310        320        330        340        350        360 
TELLDDPWIT SKRVETSNIL PDVKKGFSLR KKLRDAIEIV KLNNRIKRLR NMYSLGDDGD 

       370        380        390        400        410        420 
NDIEENSLNE SLLDGVTHSL DDLRLQSQKK GGELTEEQMK LKSALTKDAF VQIVKAATKN 

       430        440 
KHKVLAGEEE DDSKKTLHDD RESKSED 

« Hide

References

« Hide 'large scale' references
[1]"Multiple Ca2+/calmodulin-dependent protein kinase genes in a unicellular eukaryote."
Pausch M.H., Kaim D., Kunisawa R., Admon A., Thorner J.
EMBO J. 10:1511-1522(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Two yeast genes encoding calmodulin-dependent protein kinases. Isolation, sequencing and bacterial expressions of CMK1 and CMK2."
Ohya Y., Kawasaki H., Suzuki K., Londesborough J., Anraku Y.
J. Biol. Chem. 266:12784-12794(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-367, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-443, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-353; SER-354; SER-367 AND SER-371, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X56961 Genomic DNA. Translation: CAA40281.1.
D90376 Genomic DNA. Translation: BAA14384.1.
Z74758 Genomic DNA. Translation: CAA99015.1.
BK006948 Genomic DNA. Translation: DAA10766.1.
PIRB40896.
RefSeqNP_014626.1. NM_001183270.1.

3D structure databases

ProteinModelPortalP22517.
SMRP22517. Positions 1-371.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34387. 101 interactions.
DIPDIP-2306N.
IntActP22517. 6 interactions.
MINTMINT-481301.
STRING4932.YOL016C.

Proteomic databases

PaxDbP22517.
PeptideAtlasP22517.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOL016C; YOL016C; YOL016C.
GeneID854144.
KEGGsce:YOL016C.

Organism-specific databases

CYGDYOL016c.
SGDS000005376. CMK2.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00750000117487.
HOGENOMHOG000233016.
KOK08794.
OMAWDNISID.
OrthoDBEOG793BK1.

Enzyme and pathway databases

BioCycYEAST:G3O-33432-MONOMER.
BRENDA2.7.11.17. 984.

Gene expression databases

GenevestigatorP22517.

Family and domain databases

InterProIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERPTHR24347. PTHR24347. 1 hit.
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio975888.
PROP22517.

Entry information

Entry nameKCC2_YEAST
AccessionPrimary (citable) accession number: P22517
Secondary accession number(s): D6W250
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families