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P22517

- KCC2_YEAST

UniProt

P22517 - KCC2_YEAST

Protein

Calcium/calmodulin-dependent protein kinase II

Gene

CMK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Important in cellular regulation.

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei76 – 761ATPPROSITE-ProRule annotation
    Active sitei171 – 1711Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi53 – 619ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calmodulin-dependent protein kinase activity Source: SGD

    GO - Biological processi

    1. protein phosphorylation Source: SGD
    2. signal transduction Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calmodulin-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33432-MONOMER.
    BRENDAi2.7.11.17. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium/calmodulin-dependent protein kinase II (EC:2.7.11.17)
    Gene namesi
    Name:CMK2
    Ordered Locus Names:YOL016C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOL016c.
    SGDiS000005376. CMK2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 447447Calcium/calmodulin-dependent protein kinase IIPRO_0000086105Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei316 – 3161Phosphothreonine; by autocatalysisBy similarity
    Modified residuei353 – 3531Phosphotyrosine1 Publication
    Modified residuei354 – 3541Phosphoserine1 Publication
    Modified residuei367 – 3671Phosphoserine2 Publications
    Modified residuei371 – 3711Phosphoserine1 Publication
    Modified residuei387 – 3871Phosphoserine; by autocatalysisBy similarity
    Modified residuei443 – 4431Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP22517.
    PaxDbiP22517.
    PeptideAtlasiP22517.

    Expressioni

    Gene expression databases

    GenevestigatoriP22517.

    Interactioni

    Subunit structurei

    Multimeric.

    Protein-protein interaction databases

    BioGridi34387. 101 interactions.
    DIPiDIP-2306N.
    IntActiP22517. 6 interactions.
    MINTiMINT-481301.
    STRINGi4932.YOL016C.

    Structurei

    3D structure databases

    ProteinModelPortaliP22517.
    SMRiP22517. Positions 32-371.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini47 – 309263Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni323 – 33412Calmodulin-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117487.
    HOGENOMiHOG000233016.
    KOiK08794.
    OMAiWDNISID.
    OrthoDBiEOG793BK1.

    Family and domain databases

    InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24347. PTHR24347. 1 hit.
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P22517-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPKESEVINS EFHVDVQDPE RLNGHPVAKF INKLSGQPES YVNRTNYIFG    50
    RTLGAGSFGV VRQARKLSTN EDVAIKILLK KALQGNNVQL QMLYEELSIL 100
    QKLSHPNIVS FKDWFESKDK FYIVTQLATG GELFDRILSR GKFTEVDAVE 150
    IIVQILGAVE YMHSKNVVHR DLKPENVLYV DKSENSPLVI ADFGIAKQLK 200
    GEEDLIYKAA GSLGYVAPEV LTQDGHGKPC DIWSIGVITY TLLCGYSPFI 250
    AESVEGFMEE CTASRYPVTF HMPYWDNISI DVKRFILKAL RLNPADRPTA 300
    TELLDDPWIT SKRVETSNIL PDVKKGFSLR KKLRDAIEIV KLNNRIKRLR 350
    NMYSLGDDGD NDIEENSLNE SLLDGVTHSL DDLRLQSQKK GGELTEEQMK 400
    LKSALTKDAF VQIVKAATKN KHKVLAGEEE DDSKKTLHDD RESKSED 447
    Length:447
    Mass (Da):50,447
    Last modified:November 1, 1997 - v2
    Checksum:i5990740B980B8312
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti282 – 2821V → A in CAA40281. (PubMed:2026147)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56961 Genomic DNA. Translation: CAA40281.1.
    D90376 Genomic DNA. Translation: BAA14384.1.
    Z74758 Genomic DNA. Translation: CAA99015.1.
    BK006948 Genomic DNA. Translation: DAA10766.1.
    PIRiB40896.
    RefSeqiNP_014626.1. NM_001183270.1.

    Genome annotation databases

    EnsemblFungiiYOL016C; YOL016C; YOL016C.
    GeneIDi854144.
    KEGGisce:YOL016C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56961 Genomic DNA. Translation: CAA40281.1 .
    D90376 Genomic DNA. Translation: BAA14384.1 .
    Z74758 Genomic DNA. Translation: CAA99015.1 .
    BK006948 Genomic DNA. Translation: DAA10766.1 .
    PIRi B40896.
    RefSeqi NP_014626.1. NM_001183270.1.

    3D structure databases

    ProteinModelPortali P22517.
    SMRi P22517. Positions 32-371.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34387. 101 interactions.
    DIPi DIP-2306N.
    IntActi P22517. 6 interactions.
    MINTi MINT-481301.
    STRINGi 4932.YOL016C.

    Proteomic databases

    MaxQBi P22517.
    PaxDbi P22517.
    PeptideAtlasi P22517.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOL016C ; YOL016C ; YOL016C .
    GeneIDi 854144.
    KEGGi sce:YOL016C.

    Organism-specific databases

    CYGDi YOL016c.
    SGDi S000005376. CMK2.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117487.
    HOGENOMi HOG000233016.
    KOi K08794.
    OMAi WDNISID.
    OrthoDBi EOG793BK1.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33432-MONOMER.
    BRENDAi 2.7.11.17. 984.

    Miscellaneous databases

    NextBioi 975888.
    PROi P22517.

    Gene expression databases

    Genevestigatori P22517.

    Family and domain databases

    InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24347. PTHR24347. 1 hit.
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Multiple Ca2+/calmodulin-dependent protein kinase genes in a unicellular eukaryote."
      Pausch M.H., Kaim D., Kunisawa R., Admon A., Thorner J.
      EMBO J. 10:1511-1522(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Two yeast genes encoding calmodulin-dependent protein kinases. Isolation, sequencing and bacterial expressions of CMK1 and CMK2."
      Ohya Y., Kawasaki H., Suzuki K., Londesborough J., Anraku Y.
      J. Biol. Chem. 266:12784-12794(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-367, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-443, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-353; SER-354; SER-367 AND SER-371, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiKCC2_YEAST
    AccessioniPrimary (citable) accession number: P22517
    Secondary accession number(s): D6W250
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 142 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7500 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3