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Protein

ATP-dependent DNA helicase CHL1

Gene

CHL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent DNA helicase important for chromosome transmission and normal cell cycle progression in G2/M (PubMed:10931920). May have a role in changing DNA topology to allow the loading of proteins involved in maintaining sister chromatid cohesion in the vicinity of the centromeres (PubMed:15020404, PubMed:14742714). Has a specific role in chromosome segregation during meiosis II (PubMed:15226378).4 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi42 – 498ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent DNA helicase activity Source: InterPro
  • DNA binding Source: UniProtKB-KW
  • DNA helicase activity Source: SGD

GO - Biological processi

  • DNA duplex unwinding Source: GOC
  • establishment of sister chromatid cohesion Source: SGD
  • interstrand cross-link repair Source: SGD
  • mitotic sister chromatid cohesion Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33927-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase CHL1 (EC:3.6.4.121 Publication)
Alternative name(s):
Chromosome loss protein 1
Chromosome transmission fidelity protein 1
Gene namesi
Name:CHL1
Synonyms:CTF1
Ordered Locus Names:YPL008W
ORF Names:YP8132.05
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL008W.
SGDiS000005929. CHL1.

Subcellular locationi

GO - Cellular componenti

  • nuclear chromatin Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi47 – 471G → A or V: No ATPase activity; increase in chromosome missegregation. 1 Publication
Mutagenesisi48 – 481K → R: No ATPase activity; increase in chromosome missegregation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 861861ATP-dependent DNA helicase CHL1PRO_0000055138Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei86 – 861PhosphoserineCombined sources
Modified residuei172 – 1721PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP22516.
PeptideAtlasiP22516.

PTM databases

iPTMnetiP22516.

Interactioni

Subunit structurei

Interacts with ECO1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ECO1P436052EBI-4600,EBI-22988

Protein-protein interaction databases

BioGridi36169. 249 interactions.
DIPiDIP-4044N.
IntActiP22516. 4 interactions.
MINTiMINT-526050.

Structurei

3D structure databases

ProteinModelPortaliP22516.
SMRiP22516. Positions 12-58, 350-828.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 458453Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi393 – 3964DEAH box

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000063199.
HOGENOMiHOG000241266.
InParanoidiP22516.
KOiK11273.
OMAiIQSEFMS.
OrthoDBiEOG7QC84W.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR006555. ATP-dep_Helicase_C.
IPR010614. DEAD_2.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR013020. DNA_helicase_DNA-repair_Rad3.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR006554. Helicase-like_DEXD_c2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF06733. DEAD_2. 1 hit.
PF13307. Helicase_C_2. 1 hit.
[Graphical view]
SMARTiSM00488. DEXDc2. 1 hit.
SM00491. HELICc2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00604. rad3. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51193. HELICASE_ATP_BIND_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22516-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKKEYSETF YHPYKPYDIQ VQLMETVYRV LSEGKKIAIL ESPTGTGKTL
60 70 80 90 100
SLICATMTWL RMNKADIFTR METNIKTNED DSENLSDDEP DWVIDTYRKS
110 120 130 140 150
VLQEKVDLLN DYEKHLNEIN TTSCKQLKTM CDLDKEHGRY KSVDPLRKKR
160 170 180 190 200
KGARHLDVSL EEQDFIPRPY ESDSENNDTS KSTRGGRISD KDYKLSELNS
210 220 230 240 250
QIITLLDKID GKVSRDPNNG DRFDVTNQNP VKIYYASRTY SQLGQFTSQL
260 270 280 290 300
RLPSFPSSFR DKVPDEKVKY LPLASKKQLC INPKVMKWKT LEAINDACAD
310 320 330 340 350
LRHSKEGCIF YQNTNEWRHC PDTLALRDMI FSEIQDIEDL VPLGKSLGIC
360 370 380 390 400
PYYASREALP IAEVVTLPYQ YLLSESTRSS LQINLENSIV IIDEAHNLIE
410 420 430 440 450
TINSIYSSQI SLEDLKNCHK GIVTYFNKFK SRLNPGNRVN LLKLNSLLMT
460 470 480 490 500
LIQFIVKNFK KIGQEIDPND MFTGSNIDTL NIHKLLRYIK VSKIAYKIDT
510 520 530 540 550
YNQALKEEES SKNENPIKET HKKSVSSQPL LFKVSQFLYC LTNLTSEGQF
560 570 580 590 600
FFEKNYSIKY MLLEPSKPFE SILNQAKCVV LAGGTMEPMS EFLSNLLPEV
610 620 630 640 650
PSEDITTLSC NHVIPKENLQ TYITNQPELE FTFEKRMSPS LVNNHLFQFF
660 670 680 690 700
VDLSKAVPKK GGIVAFFPSY QYLAHVIQCW KQNDRFATLN NVRKIFYEAK
710 720 730 740 750
DGDDILSGYS DSVAEGRGSL LLAIVGGKLS EGINFQDDLC RAVVMVGLPF
760 770 780 790 800
PNIFSGELIV KRKHLAAKIM KSGGTEEEAS RATKEFMENI CMKAVNQSVG
810 820 830 840 850
RAIRHANDYA NIYLLDVRYN RPNFRKKLSR WVQDSINSEH TTHQVISSTR
860
KFFSMRSLNS R
Length:861
Mass (Da):98,806
Last modified:August 1, 1991 - v1
Checksum:i15D0CC397A77F8F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33335 Genomic DNA. Translation: AAB68097.1.
X56584 Genomic DNA. Translation: CAA39922.1.
Z48483 Genomic DNA. Translation: CAA88378.1.
Z71255 Genomic DNA. Translation: CAA95033.1.
BK006949 Genomic DNA. Translation: DAA11420.1.
PIRiS12499.
RefSeqiNP_015317.1. NM_001183822.1.

Genome annotation databases

EnsemblFungiiYPL008W; YPL008W; YPL008W.
GeneIDi856099.
KEGGisce:YPL008W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33335 Genomic DNA. Translation: AAB68097.1.
X56584 Genomic DNA. Translation: CAA39922.1.
Z48483 Genomic DNA. Translation: CAA88378.1.
Z71255 Genomic DNA. Translation: CAA95033.1.
BK006949 Genomic DNA. Translation: DAA11420.1.
PIRiS12499.
RefSeqiNP_015317.1. NM_001183822.1.

3D structure databases

ProteinModelPortaliP22516.
SMRiP22516. Positions 12-58, 350-828.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36169. 249 interactions.
DIPiDIP-4044N.
IntActiP22516. 4 interactions.
MINTiMINT-526050.

PTM databases

iPTMnetiP22516.

Proteomic databases

MaxQBiP22516.
PeptideAtlasiP22516.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL008W; YPL008W; YPL008W.
GeneIDi856099.
KEGGisce:YPL008W.

Organism-specific databases

EuPathDBiFungiDB:YPL008W.
SGDiS000005929. CHL1.

Phylogenomic databases

GeneTreeiENSGT00530000063199.
HOGENOMiHOG000241266.
InParanoidiP22516.
KOiK11273.
OMAiIQSEFMS.
OrthoDBiEOG7QC84W.

Enzyme and pathway databases

BioCyciYEAST:G3O-33927-MONOMER.

Miscellaneous databases

NextBioi981143.
PROiP22516.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR006555. ATP-dep_Helicase_C.
IPR010614. DEAD_2.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR013020. DNA_helicase_DNA-repair_Rad3.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR006554. Helicase-like_DEXD_c2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF06733. DEAD_2. 1 hit.
PF13307. Helicase_C_2. 1 hit.
[Graphical view]
SMARTiSM00488. DEXDc2. 1 hit.
SM00491. HELICc2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00604. rad3. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51193. HELICASE_ATP_BIND_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The CHL 1 (CTF 1) gene product of Saccharomyces cerevisiae is important for chromosome transmission and normal cell cycle progression in G2/M."
    Gerring S.L., Spencer F., Hieter P.
    EMBO J. 9:4347-4358(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "CHL1 is a nuclear protein with an essential ATP binding site that exhibits a size-dependent effect on chromosome segregation."
    Holloway S.L.
    Nucleic Acids Res. 28:3056-3064(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-47 AND LYS-48.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Chl1p, a DNA helicase-like protein in budding yeast, functions in sister-chromatid cohesion."
    Skibbens R.V.
    Genetics 166:33-42(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ECO1, SUBCELLULAR LOCATION.
  7. "Sister-chromatid cohesion mediated by the alternative RF-CCtf18/Dcc1/Ctf8, the helicase Chl1 and the polymerase-alpha-associated protein Ctf4 is essential for chromatid disjunction during meiosis II."
    Petronczki M., Chwalla B., Siomos M.F., Yokobayashi S., Helmhart W., Deutschbauer A.M., Davis R.W., Watanabe Y., Nasmyth K.
    J. Cell Sci. 117:3547-3559(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. "Identification of protein complexes required for efficient sister chromatid cohesion."
    Mayer M.L., Pot I., Chang M., Xu H., Aneliunas V., Kwok T., Newitt R., Aebersold R., Boone C., Brown G.W., Hieter P.
    Mol. Biol. Cell 15:1736-1745(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-86 AND SER-172, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCHL1_YEAST
AccessioniPrimary (citable) accession number: P22516
Secondary accession number(s): D6W404
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: May 11, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 98 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.