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Protein

ATP-dependent DNA helicase CHL1

Gene

CHL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent DNA helicase important for chromosome transmission and normal cell cycle progression in G2/M (PubMed:10931920). May have a role in changing DNA topology to allow the loading of proteins involved in maintaining sister chromatid cohesion in the vicinity of the centromeres (PubMed:15020404, PubMed:14742714). Has a specific role in chromosome segregation during meiosis II (PubMed:15226378).4 Publications

Miscellaneous

Present with 98 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi42 – 49ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent DNA helicase activity Source: InterPro
  • DNA binding Source: UniProtKB-KW
  • DNA helicase activity Source: SGD

GO - Biological processi

  • establishment of sister chromatid cohesion Source: SGD
  • interstrand cross-link repair Source: SGD
  • mitotic sister chromatid cohesion Source: SGD

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33927-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase CHL1 (EC:3.6.4.121 Publication)
Alternative name(s):
Chromosome loss protein 1
Chromosome transmission fidelity protein 1
Gene namesi
Name:CHL1
Synonyms:CTF1
Ordered Locus Names:YPL008W
ORF Names:YP8132.05
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL008W.
SGDiS000005929. CHL1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi47G → A or V: No ATPase activity; increase in chromosome missegregation. 1 Publication1
Mutagenesisi48K → R: No ATPase activity; increase in chromosome missegregation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551381 – 861ATP-dependent DNA helicase CHL1Add BLAST861

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei86PhosphoserineCombined sources1
Modified residuei172PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP22516.
PRIDEiP22516.

PTM databases

iPTMnetiP22516.

Interactioni

Subunit structurei

Interacts with ECO1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ECO1P436052EBI-4600,EBI-22988

Protein-protein interaction databases

BioGridi36169. 435 interactors.
DIPiDIP-4044N.
IntActiP22516. 4 interactors.
MINTiMINT-526050.
STRINGi4932.YPL008W.

Structurei

3D structure databases

ProteinModelPortaliP22516.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 458Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST453

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi393 – 396DEAH box4

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00530000063199.
HOGENOMiHOG000241266.
InParanoidiP22516.
KOiK11273.
OMAiFKVQRYC.
OrthoDBiEOG092C10ZG.

Family and domain databases

InterProiView protein in InterPro
IPR006555. ATP-dep_Helicase_C.
IPR010614. DEAD_2.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR013020. DNA_helicase_DNA-repair_Rad3.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR006554. Helicase-like_DEXD_c2.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF06733. DEAD_2. 1 hit.
PF13307. Helicase_C_2. 1 hit.
SMARTiView protein in SMART
SM00488. DEXDc2. 1 hit.
SM00491. HELICc2. 1 hit.
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00604. rad3. 1 hit.
PROSITEiView protein in PROSITE
PS00690. DEAH_ATP_HELICASE. 1 hit.
PS51193. HELICASE_ATP_BIND_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P22516-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKKEYSETF YHPYKPYDIQ VQLMETVYRV LSEGKKIAIL ESPTGTGKTL
60 70 80 90 100
SLICATMTWL RMNKADIFTR METNIKTNED DSENLSDDEP DWVIDTYRKS
110 120 130 140 150
VLQEKVDLLN DYEKHLNEIN TTSCKQLKTM CDLDKEHGRY KSVDPLRKKR
160 170 180 190 200
KGARHLDVSL EEQDFIPRPY ESDSENNDTS KSTRGGRISD KDYKLSELNS
210 220 230 240 250
QIITLLDKID GKVSRDPNNG DRFDVTNQNP VKIYYASRTY SQLGQFTSQL
260 270 280 290 300
RLPSFPSSFR DKVPDEKVKY LPLASKKQLC INPKVMKWKT LEAINDACAD
310 320 330 340 350
LRHSKEGCIF YQNTNEWRHC PDTLALRDMI FSEIQDIEDL VPLGKSLGIC
360 370 380 390 400
PYYASREALP IAEVVTLPYQ YLLSESTRSS LQINLENSIV IIDEAHNLIE
410 420 430 440 450
TINSIYSSQI SLEDLKNCHK GIVTYFNKFK SRLNPGNRVN LLKLNSLLMT
460 470 480 490 500
LIQFIVKNFK KIGQEIDPND MFTGSNIDTL NIHKLLRYIK VSKIAYKIDT
510 520 530 540 550
YNQALKEEES SKNENPIKET HKKSVSSQPL LFKVSQFLYC LTNLTSEGQF
560 570 580 590 600
FFEKNYSIKY MLLEPSKPFE SILNQAKCVV LAGGTMEPMS EFLSNLLPEV
610 620 630 640 650
PSEDITTLSC NHVIPKENLQ TYITNQPELE FTFEKRMSPS LVNNHLFQFF
660 670 680 690 700
VDLSKAVPKK GGIVAFFPSY QYLAHVIQCW KQNDRFATLN NVRKIFYEAK
710 720 730 740 750
DGDDILSGYS DSVAEGRGSL LLAIVGGKLS EGINFQDDLC RAVVMVGLPF
760 770 780 790 800
PNIFSGELIV KRKHLAAKIM KSGGTEEEAS RATKEFMENI CMKAVNQSVG
810 820 830 840 850
RAIRHANDYA NIYLLDVRYN RPNFRKKLSR WVQDSINSEH TTHQVISSTR
860
KFFSMRSLNS R
Length:861
Mass (Da):98,806
Last modified:August 1, 1991 - v1
Checksum:i15D0CC397A77F8F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33335 Genomic DNA. Translation: AAB68097.1.
X56584 Genomic DNA. Translation: CAA39922.1.
Z48483 Genomic DNA. Translation: CAA88378.1.
Z71255 Genomic DNA. Translation: CAA95033.1.
BK006949 Genomic DNA. Translation: DAA11420.1.
PIRiS12499.
RefSeqiNP_015317.1. NM_001183822.1.

Genome annotation databases

EnsemblFungiiYPL008W; YPL008W; YPL008W.
GeneIDi856099.
KEGGisce:YPL008W.

Similar proteinsi

Entry informationi

Entry nameiCHL1_YEAST
AccessioniPrimary (citable) accession number: P22516
Secondary accession number(s): D6W404
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: November 22, 2017
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names