Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent DNA helicase CHL1

Gene

CHL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent DNA helicase important for chromosome transmission and normal cell cycle progression in G2/M (PubMed:10931920). May have a role in changing DNA topology to allow the loading of proteins involved in maintaining sister chromatid cohesion in the vicinity of the centromeres (PubMed:15020404, PubMed:14742714). Has a specific role in chromosome segregation during meiosis II (PubMed:15226378).4 Publications

Miscellaneous

Present with 98 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi42 – 49ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent DNA helicase activity Source: InterPro
  • DNA binding Source: UniProtKB-KW
  • DNA helicase activity Source: SGD

GO - Biological processi

  • establishment of sister chromatid cohesion Source: SGD
  • interstrand cross-link repair Source: SGD
  • mitotic sister chromatid cohesion Source: SGD

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33927-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase CHL1 (EC:3.6.4.121 Publication)
Alternative name(s):
Chromosome loss protein 1
Chromosome transmission fidelity protein 1
Gene namesi
Name:CHL1
Synonyms:CTF1
Ordered Locus Names:YPL008W
ORF Names:YP8132.05
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL008W
SGDiS000005929 CHL1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi47G → A or V: No ATPase activity; increase in chromosome missegregation. 1 Publication1
Mutagenesisi48K → R: No ATPase activity; increase in chromosome missegregation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551381 – 861ATP-dependent DNA helicase CHL1Add BLAST861

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei86PhosphoserineCombined sources1
Modified residuei172PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP22516
PaxDbiP22516
PRIDEiP22516

PTM databases

iPTMnetiP22516

Interactioni

Subunit structurei

Interacts with ECO1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ECO1P436052EBI-4600,EBI-22988

Protein-protein interaction databases

BioGridi36169444 interactors.
DIPiDIP-4044N
IntActiP22516 11 interactors.
MINTiP22516
STRINGi4932.YPL008W

Structurei

3D structure databases

ProteinModelPortaliP22516
SMRiP22516
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 458Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST453

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi393 – 396DEAH box4

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00530000063199
HOGENOMiHOG000241266
InParanoidiP22516
KOiK11273
OMAiFKVQRYC
OrthoDBiEOG092C10ZG

Family and domain databases

InterProiView protein in InterPro
IPR006555 ATP-dep_Helicase_C
IPR010614 DEAD_2
IPR002464 DNA/RNA_helicase_DEAH_CS
IPR014013 Helic_SF1/SF2_ATP-bd_DinG/Rad3
IPR006554 Helicase-like_DEXD_c2
IPR027417 P-loop_NTPase
IPR013020 Rad3/Chl1-like
PfamiView protein in Pfam
PF06733 DEAD_2, 1 hit
PF13307 Helicase_C_2, 1 hit
SMARTiView protein in SMART
SM00488 DEXDc2, 1 hit
SM00491 HELICc2, 1 hit
SUPFAMiSSF52540 SSF52540, 3 hits
TIGRFAMsiTIGR00604 rad3, 1 hit
PROSITEiView protein in PROSITE
PS00690 DEAH_ATP_HELICASE, 1 hit
PS51193 HELICASE_ATP_BIND_2, 1 hit

Sequencei

Sequence statusi: Complete.

P22516-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKKEYSETF YHPYKPYDIQ VQLMETVYRV LSEGKKIAIL ESPTGTGKTL
60 70 80 90 100
SLICATMTWL RMNKADIFTR METNIKTNED DSENLSDDEP DWVIDTYRKS
110 120 130 140 150
VLQEKVDLLN DYEKHLNEIN TTSCKQLKTM CDLDKEHGRY KSVDPLRKKR
160 170 180 190 200
KGARHLDVSL EEQDFIPRPY ESDSENNDTS KSTRGGRISD KDYKLSELNS
210 220 230 240 250
QIITLLDKID GKVSRDPNNG DRFDVTNQNP VKIYYASRTY SQLGQFTSQL
260 270 280 290 300
RLPSFPSSFR DKVPDEKVKY LPLASKKQLC INPKVMKWKT LEAINDACAD
310 320 330 340 350
LRHSKEGCIF YQNTNEWRHC PDTLALRDMI FSEIQDIEDL VPLGKSLGIC
360 370 380 390 400
PYYASREALP IAEVVTLPYQ YLLSESTRSS LQINLENSIV IIDEAHNLIE
410 420 430 440 450
TINSIYSSQI SLEDLKNCHK GIVTYFNKFK SRLNPGNRVN LLKLNSLLMT
460 470 480 490 500
LIQFIVKNFK KIGQEIDPND MFTGSNIDTL NIHKLLRYIK VSKIAYKIDT
510 520 530 540 550
YNQALKEEES SKNENPIKET HKKSVSSQPL LFKVSQFLYC LTNLTSEGQF
560 570 580 590 600
FFEKNYSIKY MLLEPSKPFE SILNQAKCVV LAGGTMEPMS EFLSNLLPEV
610 620 630 640 650
PSEDITTLSC NHVIPKENLQ TYITNQPELE FTFEKRMSPS LVNNHLFQFF
660 670 680 690 700
VDLSKAVPKK GGIVAFFPSY QYLAHVIQCW KQNDRFATLN NVRKIFYEAK
710 720 730 740 750
DGDDILSGYS DSVAEGRGSL LLAIVGGKLS EGINFQDDLC RAVVMVGLPF
760 770 780 790 800
PNIFSGELIV KRKHLAAKIM KSGGTEEEAS RATKEFMENI CMKAVNQSVG
810 820 830 840 850
RAIRHANDYA NIYLLDVRYN RPNFRKKLSR WVQDSINSEH TTHQVISSTR
860
KFFSMRSLNS R
Length:861
Mass (Da):98,806
Last modified:August 1, 1991 - v1
Checksum:i15D0CC397A77F8F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33335 Genomic DNA Translation: AAB68097.1
X56584 Genomic DNA Translation: CAA39922.1
Z48483 Genomic DNA Translation: CAA88378.1
Z71255 Genomic DNA Translation: CAA95033.1
BK006949 Genomic DNA Translation: DAA11420.1
PIRiS12499
RefSeqiNP_015317.1, NM_001183822.1

Genome annotation databases

EnsemblFungiiYPL008W; YPL008W; YPL008W
GeneIDi856099
KEGGisce:YPL008W

Similar proteinsi

Entry informationi

Entry nameiCHL1_YEAST
AccessioniPrimary (citable) accession number: P22516
Secondary accession number(s): D6W404
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: March 28, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome