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Protein

Glyceraldehyde-3-phosphate dehydrogenase, glycosomal

Gene
N/A
Organism
Trypanosoma cruzi
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gGAPDH), Glyceraldehyde-3-phosphate dehydrogenase (gGAPDH), Glyceraldehyde-3-phosphate dehydrogenase, glycosomal
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381NAD2 Publications
Binding sitei91 – 911NAD; via carbonyl oxygen2 Publications
Binding sitei134 – 1341NAD2 Publications
Active sitei166 – 1661Nucleophile
Sitei194 – 1941Activates thiol group during catalysis
Binding sitei197 – 1971Glyceraldehyde 3-phosphate
Binding sitei249 – 2491Glyceraldehyde 3-phosphate
Binding sitei335 – 3351NAD2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 132NAD2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.2.1.12. 6524.
UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase, glycosomal (EC:1.2.1.12)
Short name:
GAPDH
OrganismiTrypanosoma cruzi
Taxonomic identifieri5693 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosomaSchizotrypanum

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Glycosome, Peroxisome

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL5926.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359Glyceraldehyde-3-phosphate dehydrogenase, glycosomalPRO_0000145532Add
BLAST

Proteomic databases

PaxDbiP22513.
PRIDEiP22513.

Interactioni

Subunit structurei

Homotetramer.3 Publications

Protein-protein interaction databases

STRINGi353153.XP_812138.1.

Chemistry

BindingDBiP22513.

Structurei

Secondary structure

1
359
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86Combined sources
Helixi12 – 2312Combined sources
Turni28 – 303Combined sources
Beta strandi31 – 377Combined sources
Helixi43 – 519Combined sources
Turni54 – 563Combined sources
Beta strandi63 – 664Combined sources
Beta strandi68 – 714Combined sources
Beta strandi72 – 743Combined sources
Beta strandi77 – 804Combined sources
Beta strandi83 – 875Combined sources
Turni94 – 963Combined sources
Helixi99 – 1024Combined sources
Beta strandi106 – 1094Combined sources
Beta strandi111 – 1133Combined sources
Helixi117 – 1204Combined sources
Helixi122 – 1254Combined sources
Beta strandi130 – 1356Combined sources
Beta strandi138 – 1403Combined sources
Turni146 – 1483Combined sources
Helixi150 – 1523Combined sources
Turni155 – 1573Combined sources
Beta strandi159 – 1624Combined sources
Helixi166 – 18015Combined sources
Beta strandi186 – 19510Combined sources
Beta strandi200 – 2045Combined sources
Helixi212 – 2143Combined sources
Helixi217 – 2193Combined sources
Beta strandi222 – 2254Combined sources
Helixi228 – 2358Combined sources
Helixi237 – 2393Combined sources
Beta strandi242 – 25110Combined sources
Beta strandi256 – 2638Combined sources
Helixi270 – 28213Combined sources
Turni283 – 2886Combined sources
Beta strandi289 – 2924Combined sources
Helixi298 – 3014Combined sources
Beta strandi306 – 3116Combined sources
Helixi312 – 3176Combined sources
Beta strandi324 – 33310Combined sources
Helixi337 – 35620Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K3TX-ray1.95A/B/C/D1-359[»]
1ML3X-ray2.50A/B/C/D1-359[»]
1QXSX-ray2.75A/B/C/D1-359[»]
3DMTX-ray2.30A/B/C/D1-359[»]
3IDSX-ray1.80A/B/C/D1-359[»]
ProteinModelPortaliP22513.
SMRiP22513. Positions 1-359.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22513.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni165 – 1673Glyceraldehyde 3-phosphate binding
Regioni226 – 2272Glyceraldehyde 3-phosphate binding

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi357 – 3593Microbody targeting signalSequence analysis

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22513-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIKVGINGF GRIGRMVFQA LCEDGLLGTE IDVVAVVDMN TDAEYFAYQM
60 70 80 90 100
RYDTVHGKFK YEVTTTKSSP SVAKDDTLVV NGHRILCVKA QRNPADLPWG
110 120 130 140 150
KLGVEYVIES TGLFTAKAAA EGHLRGGARK VVISAPASGG AKTLVMGVNH
160 170 180 190 200
HEYNPSEHHV VSNASCTTNC LAPIVHVLVK EGFGVQTGLM TTIHSYTATQ
210 220 230 240 250
KTVDGVSVKD WRGGRAAAVN IIPSTTGAAK AVGMVIPSTQ GKLTGMSFRV
260 270 280 290 300
PTPDVSVVDL TFTAARDTSI QEIDAALKRA SKTYMKGILG YTDEELVSAD
310 320 330 340 350
FINDNRSSIY DSKATLQNNL PKERRFFKIV SWYDNEWGYS HRVVDLVRHM

ASKDRSARL
Length:359
Mass (Da):39,061
Last modified:August 1, 1991 - v1
Checksum:i741ED29A4426453B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52898 Genomic DNA. Translation: CAA37080.1.
X52898 Genomic DNA. Translation: CAA37079.1.
PIRiS12565. DEUT1C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52898 Genomic DNA. Translation: CAA37080.1.
X52898 Genomic DNA. Translation: CAA37079.1.
PIRiS12565. DEUT1C.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K3TX-ray1.95A/B/C/D1-359[»]
1ML3X-ray2.50A/B/C/D1-359[»]
1QXSX-ray2.75A/B/C/D1-359[»]
3DMTX-ray2.30A/B/C/D1-359[»]
3IDSX-ray1.80A/B/C/D1-359[»]
ProteinModelPortaliP22513.
SMRiP22513. Positions 1-359.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi353153.XP_812138.1.

Chemistry

BindingDBiP22513.
ChEMBLiCHEMBL5926.

Proteomic databases

PaxDbiP22513.
PRIDEiP22513.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.
BRENDAi1.2.1.12. 6524.

Miscellaneous databases

EvolutionaryTraceiP22513.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3PG_TRYCR
AccessioniPrimary (citable) accession number: P22513
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: December 9, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

There are two identical genes that code for glycosomal GAPDH.

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.