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Protein

Glyceraldehyde-3-phosphate dehydrogenase, glycosomal

Gene
N/A
Organism
Trypanosoma cruzi
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gGAPDH), Glyceraldehyde-3-phosphate dehydrogenase (gGAPDH), Glyceraldehyde-3-phosphate dehydrogenase, glycosomal
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38NAD2 Publications1
Binding sitei91NAD; via carbonyl oxygen2 Publications1
Binding sitei134NAD2 Publications1
Active sitei166Nucleophile1
Sitei194Activates thiol group during catalysis1
Binding sitei197Glyceraldehyde 3-phosphate1
Binding sitei249Glyceraldehyde 3-phosphate1
Binding sitei335NAD2 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 13NAD2 Publications2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.2.1.12. 6524.
UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase, glycosomal (EC:1.2.1.12)
Short name:
GAPDH
OrganismiTrypanosoma cruzi
Taxonomic identifieri5693 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosomaSchizotrypanum

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Glycosome, Peroxisome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5926.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001455321 – 359Glyceraldehyde-3-phosphate dehydrogenase, glycosomalAdd BLAST359

Proteomic databases

PaxDbiP22513.
PRIDEiP22513.

Interactioni

Subunit structurei

Homotetramer.3 Publications

Protein-protein interaction databases

STRINGi353153.XP_812138.1.

Chemistry databases

BindingDBiP22513.

Structurei

Secondary structure

1359
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi12 – 23Combined sources12
Turni28 – 30Combined sources3
Beta strandi31 – 37Combined sources7
Helixi43 – 51Combined sources9
Turni54 – 56Combined sources3
Beta strandi63 – 66Combined sources4
Beta strandi68 – 71Combined sources4
Beta strandi72 – 74Combined sources3
Beta strandi77 – 80Combined sources4
Beta strandi83 – 87Combined sources5
Turni94 – 96Combined sources3
Helixi99 – 102Combined sources4
Beta strandi106 – 109Combined sources4
Beta strandi111 – 113Combined sources3
Helixi117 – 120Combined sources4
Helixi122 – 125Combined sources4
Beta strandi130 – 135Combined sources6
Beta strandi138 – 140Combined sources3
Turni146 – 148Combined sources3
Helixi150 – 152Combined sources3
Turni155 – 157Combined sources3
Beta strandi159 – 162Combined sources4
Helixi166 – 180Combined sources15
Beta strandi186 – 195Combined sources10
Beta strandi200 – 204Combined sources5
Helixi212 – 214Combined sources3
Helixi217 – 219Combined sources3
Beta strandi222 – 225Combined sources4
Helixi228 – 235Combined sources8
Helixi237 – 239Combined sources3
Beta strandi242 – 251Combined sources10
Beta strandi256 – 263Combined sources8
Helixi270 – 282Combined sources13
Turni283 – 288Combined sources6
Beta strandi289 – 292Combined sources4
Helixi298 – 301Combined sources4
Beta strandi306 – 311Combined sources6
Helixi312 – 317Combined sources6
Beta strandi324 – 333Combined sources10
Helixi337 – 356Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K3TX-ray1.95A/B/C/D1-359[»]
1ML3X-ray2.50A/B/C/D1-359[»]
1QXSX-ray2.75A/B/C/D1-359[»]
3DMTX-ray2.30A/B/C/D1-359[»]
3IDSX-ray1.80A/B/C/D1-359[»]
ProteinModelPortaliP22513.
SMRiP22513.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22513.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni165 – 167Glyceraldehyde 3-phosphate binding3
Regioni226 – 227Glyceraldehyde 3-phosphate binding2

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi357 – 359Microbody targeting signalSequence analysis3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22513-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIKVGINGF GRIGRMVFQA LCEDGLLGTE IDVVAVVDMN TDAEYFAYQM
60 70 80 90 100
RYDTVHGKFK YEVTTTKSSP SVAKDDTLVV NGHRILCVKA QRNPADLPWG
110 120 130 140 150
KLGVEYVIES TGLFTAKAAA EGHLRGGARK VVISAPASGG AKTLVMGVNH
160 170 180 190 200
HEYNPSEHHV VSNASCTTNC LAPIVHVLVK EGFGVQTGLM TTIHSYTATQ
210 220 230 240 250
KTVDGVSVKD WRGGRAAAVN IIPSTTGAAK AVGMVIPSTQ GKLTGMSFRV
260 270 280 290 300
PTPDVSVVDL TFTAARDTSI QEIDAALKRA SKTYMKGILG YTDEELVSAD
310 320 330 340 350
FINDNRSSIY DSKATLQNNL PKERRFFKIV SWYDNEWGYS HRVVDLVRHM

ASKDRSARL
Length:359
Mass (Da):39,061
Last modified:August 1, 1991 - v1
Checksum:i741ED29A4426453B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52898 Genomic DNA. Translation: CAA37080.1.
X52898 Genomic DNA. Translation: CAA37079.1.
PIRiS12565. DEUT1C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52898 Genomic DNA. Translation: CAA37080.1.
X52898 Genomic DNA. Translation: CAA37079.1.
PIRiS12565. DEUT1C.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K3TX-ray1.95A/B/C/D1-359[»]
1ML3X-ray2.50A/B/C/D1-359[»]
1QXSX-ray2.75A/B/C/D1-359[»]
3DMTX-ray2.30A/B/C/D1-359[»]
3IDSX-ray1.80A/B/C/D1-359[»]
ProteinModelPortaliP22513.
SMRiP22513.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi353153.XP_812138.1.

Chemistry databases

BindingDBiP22513.
ChEMBLiCHEMBL5926.

Proteomic databases

PaxDbiP22513.
PRIDEiP22513.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.
BRENDAi1.2.1.12. 6524.

Miscellaneous databases

EvolutionaryTraceiP22513.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3PG_TRYCR
AccessioniPrimary (citable) accession number: P22513
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: November 2, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

There are two identical genes that code for glycosomal GAPDH.

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.