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Protein

Glyceraldehyde-3-phosphate dehydrogenase, glycosomal

Gene
N/A
Organism
Trypanosoma brucei brucei
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase, cytosolic, Glyceraldehyde-3-phosphate dehydrogenase, glycosomal
  2. Phosphoglycerate kinase, cytosolic, Phosphoglycerate kinase A, Phosphoglycerate kinase A, Phosphoglycerate kinase, glycosomal, Phosphoglycerate kinase, cytosolic
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Pyruvate kinase 1 (PYK1), Pyruvate kinase 2 (PYK2)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38NAD1 Publication1
Binding sitei91NAD; via carbonyl oxygen1 Publication1
Active sitei166NucleophilePROSITE-ProRule annotation1
Sitei194Activates thiol group during catalysisBy similarity1
Binding sitei197Glyceraldehyde 3-phosphateBy similarity1
Binding sitei249Glyceraldehyde 3-phosphateBy similarity1
Binding sitei335NAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 13NAD1 Publication2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD

Enzyme and pathway databases

SABIO-RKP22512.
UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase, glycosomal (EC:1.2.1.12)
Short name:
GAPDH
OrganismiTrypanosoma brucei brucei
Taxonomic identifieri5702 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Glycosome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001455312 – 359Glyceraldehyde-3-phosphate dehydrogenase, glycosomalAdd BLAST358

Proteomic databases

PRIDEiP22512.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

Secondary structure

1359
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 9Combined sources7
Helixi13 – 23Combined sources11
Turni28 – 30Combined sources3
Beta strandi31 – 39Combined sources9
Helixi43 – 51Combined sources9
Turni54 – 56Combined sources3
Beta strandi68 – 71Combined sources4
Beta strandi77 – 80Combined sources4
Beta strandi83 – 89Combined sources7
Helixi94 – 96Combined sources3
Helixi99 – 102Combined sources4
Beta strandi106 – 109Combined sources4
Beta strandi111 – 113Combined sources3
Helixi117 – 120Combined sources4
Helixi122 – 125Combined sources4
Beta strandi130 – 135Combined sources6
Beta strandi138 – 140Combined sources3
Turni146 – 148Combined sources3
Turni155 – 157Combined sources3
Beta strandi159 – 162Combined sources4
Helixi166 – 180Combined sources15
Beta strandi186 – 194Combined sources9
Beta strandi202 – 204Combined sources3
Helixi212 – 214Combined sources3
Helixi217 – 219Combined sources3
Beta strandi222 – 225Combined sources4
Helixi228 – 235Combined sources8
Helixi237 – 239Combined sources3
Beta strandi242 – 249Combined sources8
Beta strandi256 – 263Combined sources8
Helixi270 – 282Combined sources13
Turni283 – 288Combined sources6
Beta strandi289 – 292Combined sources4
Helixi298 – 301Combined sources4
Beta strandi307 – 311Combined sources5
Helixi312 – 316Combined sources5
Beta strandi324 – 333Combined sources10
Helixi337 – 353Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2X0NX-ray3.20A/B/O/P/Q/R1-359[»]
3CVNX-ray2.00B353-359[»]
ProteinModelPortaliP22512.
SMRiP22512.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22512.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni165 – 167Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni226 – 227Glyceraldehyde 3-phosphate bindingBy similarity2

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi357 – 359Microbody targeting signalSequence analysis3

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22512-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIKVGINGF GRIGRMVFQA LCDDGLLGNE IDVVAVVDMN TDARYFAYQM
60 70 80 90 100
KYDSVHGKFK HSVSTTKSKP SVAKDDTLVV NGHRILCVKA QRNPADLPWG
110 120 130 140 150
KLGVEYVIES TGLFTVKSAA EGHLRGGARK VVISAPASGG AKTFVMGVNH
160 170 180 190 200
NNYNPREQHV VSNASCTTNC LAPLVHVLVK EGFGISTGLM TTVHSYTATQ
210 220 230 240 250
KTVDGVSVKD WRGGRAAALN IIPSTTGAAK AVGMVIPSTQ GKLTGMAFRV
260 270 280 290 300
PTADVSVVDL TFIATRDTSI KEIDAALKRA SKTYMKNILG YTDEELVSAD
310 320 330 340 350
FISDSRSSIY DSKATLQNNL PNERRFFKIV SWYDNEWGYS HRVVDLVRHM

AARDRAAKL
Length:359
Mass (Da):39,038
Last modified:January 23, 2007 - v3
Checksum:iDB0C62911A7D162C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59955 Genomic DNA. Translation: CAA42576.1.
X59955 Genomic DNA. Translation: CAA42577.1.
M26816 mRNA. Translation: AAA30198.1.
PIRiS18806. DEUT1B.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59955 Genomic DNA. Translation: CAA42576.1.
X59955 Genomic DNA. Translation: CAA42577.1.
M26816 mRNA. Translation: AAA30198.1.
PIRiS18806. DEUT1B.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2X0NX-ray3.20A/B/O/P/Q/R1-359[»]
3CVNX-ray2.00B353-359[»]
ProteinModelPortaliP22512.
SMRiP22512.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP22512.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.
SABIO-RKP22512.

Miscellaneous databases

EvolutionaryTraceiP22512.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3PG_TRYBB
AccessioniPrimary (citable) accession number: P22512
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

There are two identical genes that code for glycosomal GAPDH.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.