Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glyceraldehyde-3-phosphate dehydrogenase, glycosomal

Gene
N/A
Organism
Trypanosoma brucei brucei
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase, cytosolic, Glyceraldehyde-3-phosphate dehydrogenase, glycosomal
  2. Phosphoglycerate kinase, cytosolic, Phosphoglycerate kinase A, Phosphoglycerate kinase A, Phosphoglycerate kinase, glycosomal, Phosphoglycerate kinase, cytosolic
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Pyruvate kinase 1 (PYK1), Pyruvate kinase 2 (PYK2)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381NAD1 Publication
Binding sitei91 – 911NAD; via carbonyl oxygen1 Publication
Active sitei166 – 1661NucleophilePROSITE-ProRule annotation
Sitei194 – 1941Activates thiol group during catalysisBy similarity
Binding sitei197 – 1971Glyceraldehyde 3-phosphateBy similarity
Binding sitei249 – 2491Glyceraldehyde 3-phosphateBy similarity
Binding sitei335 – 3351NAD1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 132NAD1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD

Enzyme and pathway databases

SABIO-RKP22512.
UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase, glycosomal (EC:1.2.1.12)
Short name:
GAPDH
OrganismiTrypanosoma brucei brucei
Taxonomic identifieri5702 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Glycosome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 359358Glyceraldehyde-3-phosphate dehydrogenase, glycosomalPRO_0000145531Add
BLAST

Proteomic databases

PRIDEiP22512.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

Secondary structure

1
359
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 97Combined sources
Helixi13 – 2311Combined sources
Turni28 – 303Combined sources
Beta strandi31 – 399Combined sources
Helixi43 – 519Combined sources
Turni54 – 563Combined sources
Beta strandi68 – 714Combined sources
Beta strandi77 – 804Combined sources
Beta strandi83 – 897Combined sources
Helixi94 – 963Combined sources
Helixi99 – 1024Combined sources
Beta strandi106 – 1094Combined sources
Beta strandi111 – 1133Combined sources
Helixi117 – 1204Combined sources
Helixi122 – 1254Combined sources
Beta strandi130 – 1356Combined sources
Beta strandi138 – 1403Combined sources
Turni146 – 1483Combined sources
Turni155 – 1573Combined sources
Beta strandi159 – 1624Combined sources
Helixi166 – 18015Combined sources
Beta strandi186 – 1949Combined sources
Beta strandi202 – 2043Combined sources
Helixi212 – 2143Combined sources
Helixi217 – 2193Combined sources
Beta strandi222 – 2254Combined sources
Helixi228 – 2358Combined sources
Helixi237 – 2393Combined sources
Beta strandi242 – 2498Combined sources
Beta strandi256 – 2638Combined sources
Helixi270 – 28213Combined sources
Turni283 – 2886Combined sources
Beta strandi289 – 2924Combined sources
Helixi298 – 3014Combined sources
Beta strandi307 – 3115Combined sources
Helixi312 – 3165Combined sources
Beta strandi324 – 33310Combined sources
Helixi337 – 35317Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2X0NX-ray3.20A/B/O/P/Q/R1-359[»]
3CVNX-ray2.00B353-359[»]
ProteinModelPortaliP22512.
SMRiP22512. Positions 2-359.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22512.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni165 – 1673Glyceraldehyde 3-phosphate bindingBy similarity
Regioni226 – 2272Glyceraldehyde 3-phosphate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi357 – 3593Microbody targeting signalSequence analysis

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22512-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIKVGINGF GRIGRMVFQA LCDDGLLGNE IDVVAVVDMN TDARYFAYQM
60 70 80 90 100
KYDSVHGKFK HSVSTTKSKP SVAKDDTLVV NGHRILCVKA QRNPADLPWG
110 120 130 140 150
KLGVEYVIES TGLFTVKSAA EGHLRGGARK VVISAPASGG AKTFVMGVNH
160 170 180 190 200
NNYNPREQHV VSNASCTTNC LAPLVHVLVK EGFGISTGLM TTVHSYTATQ
210 220 230 240 250
KTVDGVSVKD WRGGRAAALN IIPSTTGAAK AVGMVIPSTQ GKLTGMAFRV
260 270 280 290 300
PTADVSVVDL TFIATRDTSI KEIDAALKRA SKTYMKNILG YTDEELVSAD
310 320 330 340 350
FISDSRSSIY DSKATLQNNL PNERRFFKIV SWYDNEWGYS HRVVDLVRHM

AARDRAAKL
Length:359
Mass (Da):39,038
Last modified:January 23, 2007 - v3
Checksum:iDB0C62911A7D162C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59955 Genomic DNA. Translation: CAA42576.1.
X59955 Genomic DNA. Translation: CAA42577.1.
M26816 mRNA. Translation: AAA30198.1.
PIRiS18806. DEUT1B.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59955 Genomic DNA. Translation: CAA42576.1.
X59955 Genomic DNA. Translation: CAA42577.1.
M26816 mRNA. Translation: AAA30198.1.
PIRiS18806. DEUT1B.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2X0NX-ray3.20A/B/O/P/Q/R1-359[»]
3CVNX-ray2.00B353-359[»]
ProteinModelPortaliP22512.
SMRiP22512. Positions 2-359.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP22512.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.
SABIO-RKP22512.

Miscellaneous databases

EvolutionaryTraceiP22512.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3PG_TRYBB
AccessioniPrimary (citable) accession number: P22512
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: December 9, 2015
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

There are two identical genes that code for glycosomal GAPDH.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.