Reviewed, UniProtKB/Swiss-Prot P22505 (BGLB_PAEPO)

Names and origin

Protein names

Recommended name:
    Beta-glucosidase B
    EC=3.2.1.21
Alternative name(s):
    Gentiobiase
    Cellobiase
    Beta-D-glucoside glucohydrolase
    Amygdalase

Gene names

Name:

bglB

Organism

Paenibacillus polymyxa (Bacillus polymyxa)

Taxonomic identifier

1406 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Paenibacillaceae › Paenibacillus

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-glucosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 448

448

Beta-glucosidase B

PRO_0000063872

Sites

Active site

167

1

Proton donor Potential

Active site

356

1

Nucleophile By similarity

Secondary structure

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448

Helix Strand Turn

Details...

Beta strand

13 – 17

5

Helix

20 – 23

4

Helix

36 – 40

5

Helix

48 – 50

3

Beta strand

53 – 55

3

Helix

59 – 72

14

Beta strand

77 – 81

5

Helix

84 – 87

4

Helix

96 – 112

17

Beta strand

115 – 123

9

Helix

127 – 131

5

Helix

134 – 136

3

Helix

139 – 154

16

Beta strand

155 – 158

4

Beta strand

160 – 166

7

Helix

168 – 176

9

Beta strand

178 – 181

4

Helix

188 – 211

24

Beta strand

216 – 223

8

Beta strand

227 – 232

6

Helix

234 – 247

14

Helix

249 – 257

9

Helix

262 – 268

7

Helix

269 – 275

7

Helix

282 – 286

5

Beta strand

292 – 296

5

Beta strand

301 – 305

5

Beta strand

308 – 312

5

Beta strand

314 – 317

4

Beta strand

321 – 323

3

Helix

332 – 344

13

Beta strand

352 – 357

6

Helix

373 – 389

17

Turn

390 – 393

4

Beta strand

396 – 402

7

Helix

410 – 415

6

Beta strand

420 – 423

4

Turn

425 – 427

3

Beta strand

430 – 432

3

Helix

434 – 445

12

Sequences

	Sequence		Length	Mass (Da)
	P22505-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 628F44507C21EF1F	FASTA	448	51,573
	10 20 30 40 50 60 MSENTFIFPA TFMWGTSTSS YQIEGGTDEG GRTPSIWDTF CQIPGKVIGG DCGDVACDHF 70 80 90 100 110 120 HHFKEDVQLM KQLGFLHYRF SVAWPRIMPA AGIINEEGLL FYEHLLDEIE LAGLIPMLTL 130 140 150 160 170 180 YHWDLPQWIE DEGGWTQRET IQHFKTYASV IMDRFGERIN WWNTINEPYC ASILGYGTGE 190 200 210 220 230 240 HAPGHENWRE AFTAAHHILM CHGIASNLHK EKGLTGKIGI TLNMEHVDAA SERPEDVAAA 250 260 270 280 290 300 IRRDGFINRW FAEPLFNGKY PEDMVEWYGT YLNGLDFVQP GDMELIQQPG DFLGINYYTR 310 320 330 340 350 360 SIIRSTNDAS LLQVEQVHME EPVTDMGWEI HPESFYKLLT RIEKDFSKGL PILITENGAA 370 380 390 400 410 420 MRDELVNGQI EDTGRHGYIE EHLKACHRFI EEGGQLKGYF VWSFLDNFEW AWGYSKRFGI 430 440 VHINYETQER TPKQSALWFK QMMAKNGF

References

[1]	"Sequences and homology analysis of two genes encoding beta-glucosidases from Bacillus polymyxa." Gonzalez-Candelas L., Ramon D., Polaina J. Gene 95:31-38(1990) [PubMed: 2123813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M60211 Genomic DNA. Translation: AAA22264.1.

PIR

JW0038.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JIE	X-ray	2.30	A	2-448	[»]
2O9P	X-ray	2.10	A	2-448	[»]
2O9R	X-ray	2.30	A	4-448	[»]
2O9T	X-ray	2.15	A	2-448	[»]
2Z1S	X-ray	2.46	A	2-448	[»]

ModBase

Search...

Protein family/group databases

CAZy

GH1. Glycoside Hydrolase Family 1.

Enzyme and pathway databases

BRENDA

3.2.1.21. 276031.

Family and domain databases

InterPro

IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR017736. Glyco_hydro_1_beta-glucosidase.
IPR017853. Glyco_hydro_catalytic_core.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.

PANTHER

PTHR10353. Glyco_hydro_1. 1 hit.

Pfam

PF00232. Glyco_hydro_1. 1 hit.
[Graphical view]

PRINTS

PR00131. GLHYDRLASE1.

TIGRFAMs

TIGR03356. BGL. 1 hit.

PROSITE

PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

BGLB_PAEPO

Accession

Primary (citable) accession number: P22505

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	February 9, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)