ID   GUN_PHAVU               Reviewed;         496 AA.
AC   P22503;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   27-MAR-2024, entry version 99.
DE   RecName: Full=Endoglucanase;
DE            EC=3.2.1.4;
DE   AltName: Full=Abscission cellulase;
DE   AltName: Full=Endo-1,4-beta-glucanase;
DE   Flags: Precursor;
OS   Phaseolus vulgaris (Kidney bean) (French bean).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Phaseolus.
OX   NCBI_TaxID=3885;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Red Kidney;
RX   PubMed=16668074; DOI=10.1104/pp.95.3.928;
RA   Tucker M.L., Milligan S.B.;
RT   "Sequence analysis and comparison of avocado fruit and bean abscission
RT   cellulases.";
RL   Plant Physiol. 95:928-933(1991).
RN   [2]
RP   SEQUENCE REVISION.
RC   STRAIN=cv. Red Kidney;
RA   Tucker M.L.;
RL   Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 359-496.
RC   STRAIN=cv. Red Kidney;
RX   PubMed=16666452; DOI=10.1104/pp.88.4.1257;
RA   Tucker M.L., Sexton R., del Campillo E., Lewis L.N.;
RT   "Bean abscission cellulase: characterization of a cDNA clone and regulation
RT   of gene expression by ethylene and auxin.";
RL   Plant Physiol. 88:1257-1262(1988).
CC   -!- FUNCTION: Involved in ripening fruit process.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- DEVELOPMENTAL STAGE: In ripening fruit.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR   EMBL; M57400; AAA02563.1; -; mRNA.
DR   PIR; T11783; T11783.
DR   RefSeq; XP_007158534.1; XM_007158472.1.
DR   AlphaFoldDB; P22503; -.
DR   SMR; P22503; -.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   EnsemblPlants; ESW30528; ESW30528; PHAVU_002G160200g.
DR   GeneID; 18638121; -.
DR   Gramene; ESW30528; ESW30528; PHAVU_002G160200g.
DR   KEGG; pvu:PHAVU_002G160200g; -.
DR   eggNOG; ENOG502QRF6; Eukaryota.
DR   OMA; IQCGSAH; -.
DR   OrthoDB; 1347382at2759; -.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0009835; P:fruit ripening; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR   PANTHER; PTHR22298:SF22; ENDOGLUCANASE 18-RELATED; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS60032; GH9_1; 1.
DR   PROSITE; PS00592; GH9_2; 1.
DR   PROSITE; PS00698; GH9_3; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cellulose degradation; Fruit ripening;
KW   Glycosidase; Hydrolase; Polysaccharide degradation; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..496
FT                   /note="Endoglucanase"
FT                   /id="PRO_0000007956"
FT   ACT_SITE        93
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT   ACT_SITE        415
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        467
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   ACT_SITE        476
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   CONFLICT        460..466
FT                   /note="DSNDRFN -> IQMTFH (in Ref. 3)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   496 AA;  54461 MW;  673A035B57267C40 CRC64;
     MGYHSVFIAV FLWSSMVCHN GLAMMDDGKL TSSSGPPNYD YADALAKAIL FFEGQRSGKL
     PSSQRVKWRE DSALSDGKLQ NVNLMGGYYD AGDNVKFGWP MAFSTSLLSW AAVEYESEIS
     SVNQLGYLQS AIRWGADFML RAHTSPTTLY TQVGDGNADH NCWERPEDMD TPRTVYKIDA
     NSPGTEVAAE YAAALSAASI VFKKIDAKYS STLLSHSKSL FDFADKNRGS YSGSCPFYCS
     YSGYQDELLW AAAWLYKASG ESKYLSYIIS NQGWSQTVSE FSWDNKFVGA QTLLTEEFYG
     GKKDLAKIKT DAESFICAVM PGSNSRQIKT TPGGLLFTRD SSNLQYTTSS TMVLFIFSRI
     LNRNHINGIN CGSSHFTASQ IRGFAKTQVE YILGKNPMKM SYMVGFGSKY PKQLHHRGSS
     IPSIKVHPAK VGCNAGLSDY YNSANPNPNT HVGAIVGGPD SNDRFNDARS DYSHAEPTTY
     INAAFVASIS ALLAKT
//