Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Endoglucanase

Gene
N/A
Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in ripening fruit process.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei415 – 4151By similarity
Active sitei466 – 4661By similarity
Active sitei476 – 4761By similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
  2. fruit ripening Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Fruit ripening, Polysaccharide degradation

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase (EC:3.2.1.4)
Alternative name(s):
Abscission cellulase
Endo-1,4-beta-glucanase
OrganismiPhaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifieri3885 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 496473EndoglucanasePRO_0000007956Add
BLAST

Expressioni

Developmental stagei

In ripening fruit.

Structurei

3D structure databases

ProteinModelPortaliP22503.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22503-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGYHSVFIAV FLWSSMVCHN GLAMMDDGKL TSSSGPPNYD YADALAKAIL
60 70 80 90 100
FFEGQRSGKL PSSQRVKWRE DSALSDGKLQ NVNLMGGYYD AGDNVKFGWP
110 120 130 140 150
MAFSTSLLSW AAVEYESEIS SVNQLGYLQS AIRWGADFML RAHTSPTTLY
160 170 180 190 200
TQVGDGNADH NCWERPEDMD TPRTVYKIDA NSPGTEVAAE YAAALSAASI
210 220 230 240 250
VFKKIDAKYS STLLSHSKSL FDFADKNRGS YSGSCPFYCS YSGYQDELLW
260 270 280 290 300
AAAWLYKASG ESKYLSYIIS NQGWSQTVSE FSWDNKFVGA QTLLTEEFYG
310 320 330 340 350
GKKDLAKIKT DAESFICAVM PGSNSRQIKT TPGGLLFTRD SSNLQYTTSS
360 370 380 390 400
TMVLFIFSRI LNRNHINGIN CGSSHFTASQ IRGFAKTQVE YILGKNPMKM
410 420 430 440 450
SYMVGFGSKY PKQLHHRGSS IPSIKVHPAK VGCNAGLSDY YNSANPNPNT
460 470 480 490
HVGAIVGGPD SNDRFNDARS DYSHAEPTTY INAAFVASIS ALLAKT
Length:496
Mass (Da):54,461
Last modified:January 31, 1996 - v2
Checksum:i673A035B57267C40
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti460 – 4667DSNDRFN → IQMTFH (PubMed:16666452).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57400 mRNA. Translation: AAA02563.1.
PIRiT11783.
RefSeqiXP_007158534.1. XM_007158472.1.

Genome annotation databases

GeneIDi18638121.
KEGGipvu:PHAVU_002G160200g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57400 mRNA. Translation: AAA02563.1.
PIRiT11783.
RefSeqiXP_007158534.1. XM_007158472.1.

3D structure databases

ProteinModelPortaliP22503.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18638121.
KEGGipvu:PHAVU_002G160200g.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis and comparison of avocado fruit and bean abscission cellulases."
    Tucker M.L., Milligan S.B.
    Plant Physiol. 95:928-933(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Red Kidney.
  2. Tucker M.L.
    Submitted (JUL-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
    Strain: cv. Red Kidney.
  3. "Bean abscission cellulase: characterization of a cDNA clone and regulation of gene expression by ethylene and auxin."
    Tucker M.L., Sexton R., del Campillo E., Lewis L.N.
    Plant Physiol. 88:1257-1262(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 359-496.
    Strain: cv. Red Kidney.

Entry informationi

Entry nameiGUN_PHAVU
AccessioniPrimary (citable) accession number: P22503
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 31, 1991
Last sequence update: January 31, 1996
Last modified: November 25, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.