P22503 (GUN_PHAVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Endoglucanase EC=3.2.1.4 Alternative name(s): Abscission cellulase Endo-1,4-beta-glucanase
Organism	Phaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifier	3885 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Phaseolus

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in ripening fruit process.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Developmental stage	In ripening fruit.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Fruit ripening Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW fruit ripening Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 496	473	Endoglucanase		PRO_0000007956
Sites
	Active site	415	1	By similarity
	Active site	466	1	By similarity
	Active site	476	1	By similarity
Experimental info
	Sequence conflict	460 – 466	7	DSNDRFN → IQMTFH Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P22503 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 673A035B57267C40	FASTA	496	54,461
	10 20 30 40 50 60 MGYHSVFIAV FLWSSMVCHN GLAMMDDGKL TSSSGPPNYD YADALAKAIL FFEGQRSGKL 70 80 90 100 110 120 PSSQRVKWRE DSALSDGKLQ NVNLMGGYYD AGDNVKFGWP MAFSTSLLSW AAVEYESEIS 130 140 150 160 170 180 SVNQLGYLQS AIRWGADFML RAHTSPTTLY TQVGDGNADH NCWERPEDMD TPRTVYKIDA 190 200 210 220 230 240 NSPGTEVAAE YAAALSAASI VFKKIDAKYS STLLSHSKSL FDFADKNRGS YSGSCPFYCS 250 260 270 280 290 300 YSGYQDELLW AAAWLYKASG ESKYLSYIIS NQGWSQTVSE FSWDNKFVGA QTLLTEEFYG 310 320 330 340 350 360 GKKDLAKIKT DAESFICAVM PGSNSRQIKT TPGGLLFTRD SSNLQYTTSS TMVLFIFSRI 370 380 390 400 410 420 LNRNHINGIN CGSSHFTASQ IRGFAKTQVE YILGKNPMKM SYMVGFGSKY PKQLHHRGSS 430 440 450 460 470 480 IPSIKVHPAK VGCNAGLSDY YNSANPNPNT HVGAIVGGPD SNDRFNDARS DYSHAEPTTY 490 INAAFVASIS ALLAKT

References

[1]	"Sequence analysis and comparison of avocado fruit and bean abscission cellulases." Tucker M.L., Milligan S.B. Plant Physiol. 95:928-933(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Red Kidney.
[2]	Tucker M.L. Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION. Strain: cv. Red Kidney.
[3]	"Bean abscission cellulase: characterization of a cDNA clone and regulation of gene expression by ethylene and auxin." Tucker M.L., Sexton R., del Campillo E., Lewis L.N. Plant Physiol. 88:1257-1262(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 359-496. Strain: cv. Red Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M57400 mRNA. Translation: AAA02563.1.
PIR	T11783.
3D structure databases
ProteinModelPortal	P22503.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN_PHAVU

Accession

Primary (citable) accession number: P22503

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	February 1, 1996
Last modified:	March 6, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program