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Protein

Beta-galactosidase

Gene

lacS

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei206Proton donorSequence analysis1
Active sitei387NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.23. 6163.
3.2.1.B26. 6163.
3.2.1.B28. 6163.
SABIO-RKP22498.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Lactase
Gene namesi
Name:lacS
Ordered Locus Names:SSO3019
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000638671 – 489Beta-galactosidaseAdd BLAST489

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei116N6-methyllysine; partial1 Publication1
Modified residuei135N6-methyllysine1 Publication1
Modified residuei273N6-methyllysine; partial1 Publication1
Modified residuei311N6-methyllysine; partial1 Publication1
Modified residuei332N6-methyllysine1 Publication1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei76Not N6-methylated1
Sitei102Not N6-methylated1
Sitei124Not N6-methylated1
Sitei138Not N6-methylated1

Keywords - PTMi

Methylation

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi273057.SSO3019.

Structurei

Secondary structure

1489
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 13Combined sources5
Helixi16 – 19Combined sources4
Helixi32 – 38Combined sources7
Helixi40 – 44Combined sources5
Helixi53 – 55Combined sources3
Helixi59 – 72Combined sources14
Beta strandi77 – 81Combined sources5
Helixi84 – 87Combined sources4
Helixi112 – 121Combined sources10
Helixi124 – 139Combined sources16
Beta strandi143 – 148Combined sources6
Helixi155 – 157Combined sources3
Helixi160 – 164Combined sources5
Helixi173 – 175Combined sources3
Helixi177 – 194Combined sources18
Turni195 – 197Combined sources3
Beta strandi199 – 205Combined sources7
Helixi207 – 215Combined sources9
Helixi218 – 220Combined sources3
Helixi229 – 251Combined sources23
Beta strandi258 – 271Combined sources14
Helixi272 – 274Combined sources3
Helixi275 – 285Combined sources11
Helixi287 – 295Combined sources9
Beta strandi297 – 299Combined sources3
Beta strandi304 – 306Combined sources3
Turni308 – 312Combined sources5
Beta strandi317 – 330Combined sources14
Beta strandi333 – 336Combined sources4
Beta strandi338 – 340Combined sources3
Beta strandi346 – 349Combined sources4
Helixi366 – 379Combined sources14
Beta strandi383 – 387Combined sources5
Helixi398 – 414Combined sources17
Beta strandi419 – 425Combined sources7
Helixi433 – 435Combined sources3
Beta strandi443 – 446Combined sources4
Turni448 – 450Combined sources3
Beta strandi453 – 455Combined sources3
Helixi457 – 468Combined sources12
Helixi473 – 478Combined sources6
Beta strandi484 – 487Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GOWX-ray2.60A/B1-489[»]
1UWIX-ray2.55A/B/C/D1-489[»]
1UWQX-ray2.02A/B1-489[»]
1UWRX-ray2.14A/B1-489[»]
1UWSX-ray1.95A/B1-489[»]
1UWTX-ray1.95A/B1-489[»]
1UWUX-ray1.95A/B1-489[»]
2CEQX-ray2.14A/B1-489[»]
2CERX-ray2.29A/B1-489[»]
4EAMX-ray1.70A/B1-489[»]
4EANX-ray1.75A/B1-489[»]
5IXEX-ray1.75A/B1-489[»]
ProteinModelPortaliP22498.
SMRiP22498.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22498.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Phylogenomic databases

eggNOGiarCOG05412. Archaea.
COG2723. LUCA.
HOGENOMiHOG000088630.
InParanoidiP22498.
KOiK01190.
OMAiTYIGWEI.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 2 hits.
PfamiPF00232. Glyco_hydro_1. 2 hits.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22498-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYSFPNSFRF GWSQAGFQSE MGTPGSEDPN TDWYKWVHDP ENMAAGLVSG
60 70 80 90 100
DLPENGPGYW GNYKTFHDNA QKMGLKIARL NVEWSRIFPN PLPRPQNFDE
110 120 130 140 150
SKQDVTEVEI NENELKRLDE YANKDALNHY REIFKDLKSR GLYFILNMYH
160 170 180 190 200
WPLPLWLHDP IRVRRGDFTG PSGWLSTRTV YEFARFSAYI AWKFDDLVDE
210 220 230 240 250
YSTMNEPNVV GGLGYVGVKS GFPPGYLSFE LSRRAMYNII QAHARAYDGI
260 270 280 290 300
KSVSKKPVGI IYANSSFQPL TDKDMEAVEM AENDNRWWFF DAIIRGEITR
310 320 330 340 350
GNEKIVRDDL KGRLDWIGVN YYTRTVVKRT EKGYVSLGGY GHGCERNSVS
360 370 380 390 400
LAGLPTSDFG WEFFPEGLYD VLTKYWNRYH LYMYVTENGI ADDADYQRPY
410 420 430 440 450
YLVSHVYQVH RAINSGADVR GYLHWSLADN YEWASGFSMR FGLLKVDYNT
460 470 480
KRLYWRPSAL VYREIATNGA ITDEIEHLNS VPPVKPLRH
Length:489
Mass (Da):56,692
Last modified:June 20, 2001 - v2
Checksum:iB56F6CE8EE4A429D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti235A → H in AAA72843 (PubMed:2121622).Curated1

Mass spectrometryi

Molecular mass is 56754.8±4.9 Da from positions 1 - 489. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34696 Genomic DNA. Translation: AAA72843.1.
AF133096 Genomic DNA. Translation: AAD21094.1.
AE006641 Genomic DNA. Translation: AAK43121.1.
PIRiB90483.
JQ0767.
RefSeqiWP_009992676.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK43121; AAK43121; SSO3019.
GeneIDi27429277.
KEGGisso:SSO3019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34696 Genomic DNA. Translation: AAA72843.1.
AF133096 Genomic DNA. Translation: AAD21094.1.
AE006641 Genomic DNA. Translation: AAK43121.1.
PIRiB90483.
JQ0767.
RefSeqiWP_009992676.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GOWX-ray2.60A/B1-489[»]
1UWIX-ray2.55A/B/C/D1-489[»]
1UWQX-ray2.02A/B1-489[»]
1UWRX-ray2.14A/B1-489[»]
1UWSX-ray1.95A/B1-489[»]
1UWTX-ray1.95A/B1-489[»]
1UWUX-ray1.95A/B1-489[»]
2CEQX-ray2.14A/B1-489[»]
2CERX-ray2.29A/B1-489[»]
4EAMX-ray1.70A/B1-489[»]
4EANX-ray1.75A/B1-489[»]
5IXEX-ray1.75A/B1-489[»]
ProteinModelPortaliP22498.
SMRiP22498.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273057.SSO3019.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK43121; AAK43121; SSO3019.
GeneIDi27429277.
KEGGisso:SSO3019.

Phylogenomic databases

eggNOGiarCOG05412. Archaea.
COG2723. LUCA.
HOGENOMiHOG000088630.
InParanoidiP22498.
KOiK01190.
OMAiTYIGWEI.

Enzyme and pathway databases

BRENDAi3.2.1.23. 6163.
3.2.1.B26. 6163.
3.2.1.B28. 6163.
SABIO-RKP22498.

Miscellaneous databases

EvolutionaryTraceiP22498.
PROiP22498.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 2 hits.
PfamiPF00232. Glyco_hydro_1. 2 hits.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGAL_SULSO
AccessioniPrimary (citable) accession number: P22498
Secondary accession number(s): Q9V2Z5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: June 20, 2001
Last modified: November 2, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

The DNA coding for this protein is not found in the complete genome of strain 98/2. Sequence in PubMed:10383958 could have originated from another strain.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.