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P22459 (KCNA4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium voltage-gated channel subfamily A member 4
Alternative name(s):
HPCN2
Voltage-gated K(+) channel HuKII
Voltage-gated potassium channel HBK4
Voltage-gated potassium channel HK1
Voltage-gated potassium channel subunit Kv1.4
Gene names
Name:KCNA4
Synonyms:KCNA4L
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length653 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

Subunit structure

Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG1, DLG2 and DLG4. Interacts with SIGMAR1 By similarity. Part of a complex containing KCNA1, KCNAB1 and LGI1 By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Domain

The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similarities

Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.4/KCNA4 sub-subfamily. [View classification]

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Dlg3Q629364EBI-631235,EBI-349596From a different organism.
DLG4P783522EBI-631235,EBI-80389
Dlg4P310163EBI-631235,EBI-375655From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 653653Potassium voltage-gated channel subfamily A member 4
PRO_0000053981

Regions

Transmembrane308 – 32619Helical; Name=Segment S1; Potential
Transmembrane371 – 39222Helical; Name=Segment S2; Potential
Transmembrane404 – 42421Helical; Name=Segment S3; Potential
Transmembrane443 – 46119Helical; Voltage-sensor; Name=Segment S4; Potential
Transmembrane478 – 49720Helical; Name=Segment S5; Potential
Transmembrane539 – 56123Helical; Name=Segment S6; Potential
Motif524 – 5296Selectivity filter By similarity
Motif651 – 6533PDZ-binding By similarity
Compositional bias62 – 654Poly-His
Compositional bias123 – 13715Poly-Glu
Compositional bias162 – 1654Poly-Gly
Compositional bias433 – 4364Poly-Gln

Amino acid modifications

Modified residue901Phosphoserine; by PKA Potential
Modified residue5991Phosphoserine; by PKA Potential
Glycosylation1811N-linked (GlcNAc...) Potential
Glycosylation3521N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict381A → R in AAA60034. Ref.1
Sequence conflict421A → R in AAA60034. Ref.1
Sequence conflict84 – 885RRRRQ → EEEAT in AAA60034. Ref.1
Sequence conflict3041S → D in AAA60034. Ref.1
Sequence conflict5421S → V in AAA60034. Ref.1
Sequence conflict6311G → A in AAA60034. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P22459 [UniParc].

Last modified November 30, 2010. Version 2.
Checksum: 5E511DB704CCA013

FASTA65373,257
        10         20         30         40         50         60 
MEVAMVSAES SGCNSHMPYG YAAQARARER ERLAHSRAAA AAAVAAATAA VEGSGGSGGG 

        70         80         90        100        110        120 
SHHHHQSRGA CTSHDPQSSR GSRRRRRQRS EKKKAHYRQS SFPHCSDLMP SGSEEKILRE 

       130        140        150        160        170        180 
LSEEEEDEEE EEEEEEEGRF YYSEDDHGDE CSYTDLLPQD EGGGGYSSVR YSDCCERVVI 

       190        200        210        220        230        240 
NVSGLRFETQ MKTLAQFPET LLGDPEKRTQ YFDPLRNEYF FDRNRPSFDA ILYYYQSGGR 

       250        260        270        280        290        300 
LKRPVNVPFD IFTEEVKFYQ LGEEALLKFR EDEGFVREEE DRALPENEFK KQIWLLFEYP 

       310        320        330        340        350        360 
ESSSPARGIA IVSVLVILIS IVIFCLETLP EFRDDRDLVM ALSAGGHGGL LNDTSAPHLE 

       370        380        390        400        410        420 
NSGHTIFNDP FFIVETVCIV WFSFEFVVRC FACPSQALFF KNIMNIIDIV SILPYFITLG 

       430        440        450        460        470        480 
TDLAQQQGGG NGQQQQAMSF AILRIIRLVR VFRIFKLSRH SKGLQILGHT LRASMRELGL 

       490        500        510        520        530        540 
LIFFLFIGVI LFSSAVYFAE ADEPTTHFQS IPDAFWWAVV TMTTVGYGDM KPITVGGKIV 

       550        560        570        580        590        600 
GSLCAIAGVL TIALPVPVIV SNFNYFYHRE TENEEQTQLT QNAVSCPYLP SNLLKKFRSS 

       610        620        630        640        650 
TSSSLGDKSE YLEMEEGVKE SLCAKEEKCQ GKGDDSETDK NNCSNAKAVE TDV 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of a human fetal skeletal muscle potassium channel cDNA related to RCK4."
Philipson L.H., Schaefer K., Lamendola J., Bell G.I., Steiner D.F.
Nucleic Acids Res. 18:7160-7160(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Skeletal muscle.
[2]"Molecular cloning and characterization of two voltage-gated K+ channel cDNAs from human ventricle."
Tamkun M.M., Knoth K.M., Walbridge J.A., Kroemer H., Roden D.M., Glover D.M.
FASEB J. 5:331-337(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Heart.
[3]"Human potassium channel genes: molecular cloning and functional expression."
Ramaswami M., Gautam M., Kamb A., Rudy B., Tanouye M.A., Mathew M.K.
Mol. Cell. Neurosci. 1:214-223(1990)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[4]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"NMR structure of inactivation gates from mammalian voltage-dependent potassium channels."
Antz C., Geyer M., Fakler B., Schott M.K., Guy H.R., Frank R., Ruppersberg J.P., Kalbitzer H.R.
Nature 385:272-275(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 1-37.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M55514 mRNA. Translation: AAA60034.1.
M60450 mRNA. Translation: AAA61275.1.
L02751 mRNA. Translation: AAA36140.1.
AC124657 Genomic DNA. No translation available.
PIRA39922.
RefSeqNP_002224.1. NM_002233.3.
UniGeneHs.592002.

3D structure databases

ProteinModelPortalP22459.
SMRP22459. Positions 1-75, 175-571.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109942. 16 interactions.
IntActP22459. 6 interactions.
MINTMINT-200816.
STRING9606.ENSP00000328511.

Chemistry

BindingDBP22459.
ChEMBLCHEMBL2362996.
GuidetoPHARMACOLOGY541.

PTM databases

PhosphoSiteP22459.

Polymorphism databases

DMDM313104127.

Proteomic databases

PaxDbP22459.
PRIDEP22459.

Protocols and materials databases

DNASU3739.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000328224; ENSP00000328511; ENSG00000182255.
GeneID3739.
KEGGhsa:3739.
UCSCuc001msk.3. human.

Organism-specific databases

CTD3739.
GeneCardsGC11M030031.
H-InvDBHIX0036152.
HGNCHGNC:6222. KCNA4.
HPACAB001977.
HPA016422.
MIM176266. gene.
neXtProtNX_P22459.
PharmGKBPA207.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1226.
HOGENOMHOG000231015.
HOVERGENHBG052230.
InParanoidP22459.
KOK04877.
OMAKKAHHRQ.
OrthoDBEOG7M0NRD.
PhylomeDBP22459.
TreeFamTF313103.

Enzyme and pathway databases

ReactomeREACT_13685. Neuronal System.

Gene expression databases

BgeeP22459.
CleanExHS_KCNA4.
GenevestigatorP22459.

Family and domain databases

Gene3D1.20.120.350. 1 hit.
1.20.5.600. 1 hit.
3.30.710.10. 1 hit.
InterProIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR020467. K_chnl_volt-dep_Kv1.4.
IPR012897. K_chnl_volt-dep_Kv1.4_TID.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERPTHR11537. PTHR11537. 1 hit.
PfamPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
PF07941. K_channel_TID. 1 hit.
[Graphical view]
PRINTSPR00169. KCHANNEL.
PR01511. KV14CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMSSF54695. SSF54695. 1 hit.
ProtoNetSearch...

Other

GeneWikiKCNA4.
GenomeRNAi3739.
NextBio14635.
PROP22459.
SOURCESearch...

Entry information

Entry nameKCNA4_HUMAN
AccessionPrimary (citable) accession number: P22459
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: November 30, 2010
Last modified: April 16, 2014
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM