P22447 (GYRB_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit B
EC=5.99.1.3

Gene names

Name:	gyrB
Ordered Locus Names:	MPN_003
ORF Names:	MP151

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129)

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	650 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01898
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01898
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01898.
Sequence similarities	Belongs to the type II topoisomerase family. Contains 1 Toprim domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 650	650	DNA gyrase subunit B HAMAP MF_01898		PRO_0000145323
Regions
	Domain	435 – 546	112	Toprim

Sequences

	Sequence		Length	Mass (Da)
	P22447 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 2882D0BE1B7A349D	FASTA	650	73,810
	10 20 30 40 50 60 MEDNNKTQAY DSSSIKILEG LEAVRKRPGM YIGSTGEEGL HHMIWEIIDN SIDEAMGGFA 70 80 90 100 110 120 STVKLTLKDN FVTIVEDDGR GIPVDIHPKT NRSTVETVFT VLHAGGKFDN DSYKVSGGLH 130 140 150 160 170 180 GVGASVVNAL SSSFKVWVAR EHQQYFLAFH NGGEVIGDLV NEGKCDKEHG TKVEFVPDFT 190 200 210 220 230 240 VMEKSDYKQT VIASRLQQLA FLNKGIQIDF VDERRQNPQS FSWKYDGGLV QYIHHLNNEK 250 260 270 280 290 300 EPLFEDIIFG EKTDTVKSVS RDESYTIKVE VAFQYNKTYN QSIFSFCNNI NTTEGGTHVE 310 320 330 340 350 360 GFRNALVKII NRFAVENKFL KETDEKITRD DICEGLTAII SIKHPNPQYE GQTKKKLGNT 370 380 390 400 410 420 EVRPLVNSIV SEIFERFMLE NPQEANAIIR KTLLAQEARR RSQEARELTR RKSPFDSGSL 430 440 450 460 470 480 PGKLADCTTR DPSISELYIV EGDSAGGTAK TGRDRYFQAI LPLRGKILNV EKSHFEQIFN 490 500 510 520 530 540 NVEISALVMA VGCGIKPDFE LEKLRYNKII IMTDADVDGA HIRTLLLTFF FRFMYPLVEQ 550 560 570 580 590 600 GNIYIAQPPL YKVSYSNKDL YMQTDVQLEE WKQQHPNLKY NLQRYKGLGE MDAIQLWETT 610 620 630 640 650 MDPKVRTLLK VTVEDASIAD KAFSLLMGDE VPPRREFIEQ NARNVKNIDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mycoplasma pneumoniae DNA gyrase genes." Colman S.D., Hu P.C., Bott K.F. Mol. Microbiol. 4:1129-1134(1990) [PubMed: 2172693] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.
[3]	"Sequence analysis of 56 kb from the genome of the bacterium Mycoplasma pneumoniae comprising the dnaA region, the atp operon and a cluster of ribosomal protein genes." Hilbert H., Himmelreich R., Plagens H., Herrmann R. Nucleic Acids Res. 24:628-639(1996) [PubMed: 8604303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-637. Strain: ATCC 29342 / M129.
[4]	"Towards a two-dimensional proteome map of Mycoplasma pneumoniae." Regula J.T., Ueberle B., Boguth G., Goerg A., Schnoelzer M., Herrmann R., Frank R. Electrophoresis 21:3765-3780(2000) [PubMed: 11271496] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: ATCC 29342 / M129.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X53555 Genomic DNA. Translation: CAA37622.1. U00089 Genomic DNA. Translation: AAB95799.1. U34816 Genomic DNA. Translation: AAC43643.1.
PIR	ISYMBP. S11767.
RefSeq	NP_109691.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P22447.
ModBase	Search...
Protein-protein interaction databases
IntAct	P22447. 5 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	877190.
GenomeReviews	Gene locus MPN_003 in contig U00089_GR.
KEGG	mpn:MPN003.
PATRIC	20021279. VBIMycPne110_0003.
Phylogenomic databases
HOGENOM	HBG304889.
OMA	MDRILAN.
PhylomeDB	P22447.
ProtClustDB	PRK05644.
Enzyme and pathway databases
BioCyc	MPNE272634:MPN003-MONOMER.
Family and domain databases
HAMAP	MF_01898. GyrB. [Tree]
InterPro	IPR003594. ATPase-like_ATP-bd. IPR011557. GyrB. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR001241. Topo_IIA. IPR013759. Topo_IIA_B/N_ab. IPR002288. Topo_IIA_B_C. IPR013506. Topo_IIA_bsu_dom2. IPR013760. Topo_IIA_cen. IPR018522. TopoIIA_CS. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 1 hit.
KO	K02470.
Pfam	PF00204. DNA_gyraseB. 1 hit. PF00986. DNA_gyraseB_C. 1 hit. PF02518. HATPase_c. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00418. TPI2FAMILY.
SMART	SM00387. HATPase_c. 1 hit. SM00433. TOP2c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01059. GyrB. 1 hit.
PROSITE	PS00177. TOPOISOMERASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYRB_MYCPN

Accession

Primary (citable) accession number: P22447

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program