P22446 (GYRA_MYCPN) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 77.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: DNA gyrase subunit A EC=5.99.1.3 | ||||||
| Gene names |
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| Organism | Mycoplasma pneumoniae (strain ATCC 29342 / M129) | ||||||
| Taxonomic identifier | 272634 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma |
Protein attributes
| Sequence length | 839 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897 |
| Catalytic activity | ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897 |
| Subunit structure | Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer. |
| Subcellular location | Cytoplasm Potential HAMAP MF_01897. |
| Sequence similarities | Belongs to the topoisomerase GyrA/ParC subunit family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm |
| Ligand | ATP-binding DNA-binding Nucleotide-binding |
| Molecular function | Isomerase Topoisomerase |
| Technical term | Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological process | DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependentInferred from electronic annotation. Source: InterPro |
| Cellular component | chromosome Inferred from electronic annotation. Source: InterPro cytoplasmInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 839 | 839 | DNA gyrase subunit A HAMAP MF_01897 | PRO_0000145241 | |||||
Sites | |||||||||
| Active site | 134 | 1 | O-(5'-phospho-DNA)-tyrosine intermediate By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129. |
| [2] | "Mycoplasma pneumoniae DNA gyrase genes." Colman S.D., Hu P.C., Bott K.F. Mol. Microbiol. 4:1129-1134(1990) [PubMed: 2172693] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-156. |
| [3] | "Towards a two-dimensional proteome map of Mycoplasma pneumoniae." Regula J.T., Ueberle B., Boguth G., Goerg A., Schnoelzer M., Herrmann R., Frank R. Electrophoresis 21:3765-3780(2000) [PubMed: 11271496] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY. Strain: ATCC 29342 / M129. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U00089 Genomic DNA. Translation: AAB95798.1. X53555 Genomic DNA. Translation: CAA37623.1. |
| PIR | S73476. |
| RefSeq | NP_109692.1. NC_000912.1. |
3D structure databases | |
| ProteinModelPortal | P22446. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P22446. 6 interactions. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 877234. |
| GenomeReviews | Gene locus MPN_004 in contig U00089_GR. |
| KEGG | mpn:MPN004. |
| PATRIC | 20021281. VBIMycPne110_0004. |
Phylogenomic databases | |
| HOGENOM | HBG635183. |
| OMA | TSIPPHR. |
| PhylomeDB | P22446. |
| ProtClustDB | PRK05560. |
Enzyme and pathway databases | |
| BioCyc | MPNE272634:MPN004-MONOMER. |
Family and domain databases | |
| HAMAP | MF_01897. GyrA. [Tree] |
| InterPro | IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013760. Topo_IIA_cen. [Graphical view] |
| Gene3D | G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 2 hits. |
| KO | K02469. |
| Pfam | PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view] |
| SMART | SM00434. TOP4c. 1 hit. [Graphical view] |
| SUPFAM | SSF56719. Topo_IIA_cen. 1 hit. |
| TIGRFAMs | TIGR01063. GyrA. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | GYRA_MYCPN | ||||||||
| Accession | Primary (citable) accession number: P22446 Secondary accession number(s): P75108 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Mycoplasma pneumoniae Mycoplasma pneumoniae (strain M129): entries and gene names |
| SIMILARITY comments Index of protein domains and families |

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