P22446 (GYRA_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	MPN_004
ORF Names:	MP150

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129)

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	839 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 839	839	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145241
Sites
	Active site	134	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P22446 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: C499618577714AFE	FASTA	839	93,357
	10 20 30 40 50 60 MAKQQDQIDK IRQELAQSAI KNISLSSELE RSFMEYAMSV IVARALPDAR DGLKPVHRRV 70 80 90 100 110 120 LYGAYTGGMH HDRPFKKSAR IVGDVMSKFH PHGDMAIYDT MSRMAQDFSL RYLLIDGHGN 130 140 150 160 170 180 FGSIDGDRPA AQRYTEARLS KLAGELLRDI DKDTVDFVAN YDGEEQEPTV LPAAFPNLLA 190 200 210 220 230 240 NGSSGIAVGM STSIPSHNLS ELIQGLILLI DNPDCTINDL LGVIKGPDFP TGANIIYTKG 250 260 270 280 290 300 IESYFETGKG NVVIRSKVSI EQLPTRAALV VTEIPYMVNK TSLIEKIVEL VKAEEITGIA 310 320 330 340 350 360 DIRDESSREG IRLVIEVKRD TVPEVLLNQL FKSTRLQVRF PVNMLALVKG APKLLNMKQA 370 380 390 400 410 420 LTVYLEHQLD VLIRKTQFNL KKYQERFHIL SGLLIAALNI DEVIAIIKKS ANNQVAMEAL 430 440 450 460 470 480 HERFGLDEIQ ARAVLDMRLR SLSVLEVNKL QTEQQELKAL IEFCQQVLAD KQLQLKLIKE 490 500 510 520 530 540 QLTKINEQFG DPRRSEILYG ISEDIDDEDL ITQENVVITM STNGYLKRIG VDAYNLQHRG 550 560 570 580 590 600 GVGVKGLTTY TDDSISQLLV CSTHSDLLFF TDKGKVYRIR AHQIPPGFRT NKGIPAVNLI 610 620 630 640 650 660 KIDKDEKICA LISVNDYQNG YFFFCTKNGT IKRTSLSEFA NILSIGKRAI LFKENDVLFS 670 680 690 700 710 720 VIRTSGQDDI FIGSTAGFVV RFHEDTVRPL SRAAMGVLGI NLNQCEFVNG LSTSSNGSLL 730 740 750 760 770 780 LSVGQNGIGK LTSIDKYRLT KRNAKGVKTL RVTAKTGPVV TTTTVFGNED LLMISSAGKI 790 800 810 820 830 VRISLEQLSE QRKNTSGVKL IKLKEKERLE TVTIFKKEEA IKTTTATETD DVGSKQITQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.
[2]	"Mycoplasma pneumoniae DNA gyrase genes." Colman S.D., Hu P.C., Bott K.F. Mol. Microbiol. 4:1129-1134(1990) [PubMed: 2172693] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-156.
[3]	"Towards a two-dimensional proteome map of Mycoplasma pneumoniae." Regula J.T., Ueberle B., Boguth G., Goerg A., Schnoelzer M., Herrmann R., Frank R. Electrophoresis 21:3765-3780(2000) [PubMed: 11271496] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY. Strain: ATCC 29342 / M129.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB95798.1. X53555 Genomic DNA. Translation: CAA37623.1.
PIR	S73476.
RefSeq	NP_109692.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P22446.
ModBase	Search...
Protein-protein interaction databases
IntAct	P22446. 6 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	877234.
GenomeReviews	Gene locus MPN_004 in contig U00089_GR.
KEGG	mpn:MPN004.
PATRIC	20021281. VBIMycPne110_0004.
Phylogenomic databases
HOGENOM	HBG635183.
OMA	TSIPPHR.
PhylomeDB	P22446.
ProtClustDB	PRK05560.
Enzyme and pathway databases
BioCyc	MPNE272634:MPN004-MONOMER.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 2 hits.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_MYCPN

Accession

Primary (citable) accession number: P22446
Secondary accession number(s): P75108

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program