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Reviewed, UniProtKB/Swiss-Prot P22446 (GYRA_MYCPN)

Last modified November 3, 2009. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    DNA gyrase subunit A
    EC=5.99.1.3
Gene names
Name: gyrA
Ordered Locus Names: MPN_004
ORF Names: MP150
OrganismMycoplasma pneumoniae [Complete proteome] [HAMAP]
Taxonomic identifier2104 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length839 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA.

Subunit structure

Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.

Sequence similarities

Belongs to the topoisomerase gyrA/parC subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 839839DNA gyrase subunit A
PRO_0000145241

Sites

Active site1341O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
P22446-1 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: C499618577714AFE

FASTA83993,357
        10         20         30         40         50         60 
MAKQQDQIDK IRQELAQSAI KNISLSSELE RSFMEYAMSV IVARALPDAR DGLKPVHRRV 

        70         80         90        100        110        120 
LYGAYTGGMH HDRPFKKSAR IVGDVMSKFH PHGDMAIYDT MSRMAQDFSL RYLLIDGHGN 

       130        140        150        160        170        180 
FGSIDGDRPA AQRYTEARLS KLAGELLRDI DKDTVDFVAN YDGEEQEPTV LPAAFPNLLA 

       190        200        210        220        230        240 
NGSSGIAVGM STSIPSHNLS ELIQGLILLI DNPDCTINDL LGVIKGPDFP TGANIIYTKG 

       250        260        270        280        290        300 
IESYFETGKG NVVIRSKVSI EQLPTRAALV VTEIPYMVNK TSLIEKIVEL VKAEEITGIA 

       310        320        330        340        350        360 
DIRDESSREG IRLVIEVKRD TVPEVLLNQL FKSTRLQVRF PVNMLALVKG APKLLNMKQA 

       370        380        390        400        410        420 
LTVYLEHQLD VLIRKTQFNL KKYQERFHIL SGLLIAALNI DEVIAIIKKS ANNQVAMEAL 

       430        440        450        460        470        480 
HERFGLDEIQ ARAVLDMRLR SLSVLEVNKL QTEQQELKAL IEFCQQVLAD KQLQLKLIKE 

       490        500        510        520        530        540 
QLTKINEQFG DPRRSEILYG ISEDIDDEDL ITQENVVITM STNGYLKRIG VDAYNLQHRG 

       550        560        570        580        590        600 
GVGVKGLTTY TDDSISQLLV CSTHSDLLFF TDKGKVYRIR AHQIPPGFRT NKGIPAVNLI 

       610        620        630        640        650        660 
KIDKDEKICA LISVNDYQNG YFFFCTKNGT IKRTSLSEFA NILSIGKRAI LFKENDVLFS 

       670        680        690        700        710        720 
VIRTSGQDDI FIGSTAGFVV RFHEDTVRPL SRAAMGVLGI NLNQCEFVNG LSTSSNGSLL 

       730        740        750        760        770        780 
LSVGQNGIGK LTSIDKYRLT KRNAKGVKTL RVTAKTGPVV TTTTVFGNED LLMISSAGKI 

       790        800        810        820        830 
VRISLEQLSE QRKNTSGVKL IKLKEKERLE TVTIFKKEEA IKTTTATETD DVGSKQITQ 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.
[2]"Mycoplasma pneumoniae DNA gyrase genes."
Colman S.D., Hu P.C., Bott K.F.
Mol. Microbiol. 4:1129-1134(1990) [PubMed: 2172693] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-156.
[3]"Towards a two-dimensional proteome map of Mycoplasma pneumoniae."
Regula J.T., Ueberle B., Boguth G., Goerg A., Schnoelzer M., Herrmann R., Frank R.
Electrophoresis 21:3765-3780(2000) [PubMed: 11271496] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

U00089 Genomic DNA. Translation: AAB95798.1.
X53555 Genomic DNA. Translation: CAA37623.1.
PIRS73476.
RefSeqNP_109692.1.

3D structure databases

HSSPHSSP built from PDB template 1AB4 based on UniProtKB P09097.
ModBaseSearch...

Genome annotation databases

GeneID877234.
GenomeReviewsGene locus MPN_004 in contig U00089_GR.
KEGGmpn:MPN004.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP22446.
OMAETSFGIN.

Enzyme and pathway databases

BioCycMPNE272634:MPN004-MON.
BRENDA5.99.1.3. 39500.

Family and domain databases

InterProIPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
[Graphical view]
Gene3DG3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit.
G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
PfamPF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
ProDomPD000742. DNA_topoisoIV. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00434. TOP4c. 1 hit.
[Graphical view]
TIGRFAMsTIGR01063. gyrA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGYRA_MYCPN
AccessionPrimary (citable) accession number: P22446
Secondary accession number(s): P75108
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: November 1, 1997
Last modified: November 3, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents