P22443 (CP19A_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 19A1
EC=1.14.14.1

Alternative name(s):
Aromatase
CYPXIX
Cytochrome P-450AROM
Estrogen synthase

Gene names

Name:	Cyp19a1
Synonyms:	Arom, Cyp19

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Membrane
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adrenal gland development Inferred from expression pattern PubMed 8097866. Source: RGD androgen metabolic process Inferred from mutant phenotype PubMed 19492405. Source: RGD bile acid metabolic process Inferred from expression pattern PubMed 20026603. Source: RGD biphenyl metabolic process Inferred from expression pattern PubMed 19464308. Source: RGD bone development Inferred from expression pattern PubMed 15012600. Source: RGD cellular response to fibroblast growth factor stimulus Inferred from expression pattern PubMed 20434519. Source: RGD cellular response to follicle-stimulating hormone stimulus Inferred from expression pattern PubMed 20810564. Source: RGD cellular response to glucose stimulus Inferred from expression pattern PubMed 18353182. Source: RGD cellular response to hydrostatic pressure Inferred from expression pattern PubMed 21047944. Source: RGD dibenzo-p-dioxin metabolic process Inferred from expression pattern PubMed 20059580. Source: RGD estrogen biosynthetic process Inferred from direct assay PubMed 19665544. Source: RGD granulocyte differentiation Inferred from expression pattern PubMed 18401763. Source: RGD hippocampus development Inferred from expression pattern PubMed 21047951. Source: RGD hypothalamus development Inferred from expression pattern PubMed 15804399. Source: RGD male gonad development Inferred from expression pattern PubMed 20554652. Source: RGD melatonin metabolic process Inferred from expression pattern PubMed 17700567. Source: RGD phthalate metabolic process Inferred from expression pattern PubMed 20920559. Source: RGD progesterone metabolic process Inferred from expression pattern PubMed 20810564. Source: RGD regulation of synapse structure and activity Inferred from mutant phenotype PubMed 17005180. Source: RGD response to cesium ion Inferred from expression pattern PubMed 20451883. Source: RGD response to drug Inferred from expression pattern PubMed 19497980. Source: RGD response to estradiol stimulus Inferred from expression pattern PubMed 21115103. Source: RGD response to herbicide Inferred from expression pattern PubMed 21126571. Source: RGD response to ionizing radiation Inferred from expression pattern PubMed 18821396. Source: RGD response to morphine Inferred from expression pattern PubMed 20977699. Source: RGD response to testosterone stimulus Inferred from expression pattern PubMed 16569475. Source: RGD
Cellular_component	cytosol Inferred from direct assay PubMed 21114983. Source: RGD dendritic spine Inferred from direct assay PubMed 14694190. Source: RGD endoplasmic reticulum Inferred from direct assay PubMed 14694190. Source: RGD membrane Inferred from electronic annotation. Source: UniProtKB-SubCell neuronal cell body Inferred from direct assay PubMed 19565659. Source: RGD synaptic vesicle Inferred from direct assay PubMed 14694190. Source: RGD terminal bouton Inferred from direct assay PubMed 20926671. Source: RGD
Molecular_function	aromatase activity Inferred from direct assay PubMed 16269516. Source: RGD electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 508	508	Cytochrome P450 19A1		PRO_0000051962
Sites
	Metal binding	437	1	Iron (heme axial ligand)
Experimental info
	Sequence conflict	3 – 4	2	LE → FQ no nucleotide entry Ref.2
	Sequence conflict	10	1	H → Q no nucleotide entry Ref.2
	Sequence conflict	88	1	W → C no nucleotide entry Ref.2
	Sequence conflict	101	1	S → L no nucleotide entry Ref.2
	Sequence conflict	123 – 129	7	QCIGMHE → VVHGHAR no nucleotide entry Ref.2
	Sequence conflict	188	1	V → L no nucleotide entry Ref.2
	Sequence conflict	210	1	E → G no nucleotide entry Ref.2
	Sequence conflict	328 – 331	4	VETA → WKQ no nucleotide entry Ref.2
	Sequence conflict	342	1	D → A no nucleotide entry Ref.2
	Sequence conflict	364 – 369	6	LRYQPV → CGISPF no nucleotide entry Ref.2
	Sequence conflict	422	1	V → L no nucleotide entry Ref.2
	Sequence conflict	425	1	R → T no nucleotide entry Ref.2
	Sequence conflict	449	1	V → I no nucleotide entry Ref.2
	Sequence conflict	466	1	R → K no nucleotide entry Ref.2
	Sequence conflict	493 – 497	5	RHIFN → SPRNS no nucleotide entry Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P22443 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: C0ADFB0FD80AB352	FASTA	508	58,412
	10 20 30 40 50 60 MFLEMLNPMH YNVTIMVPET VPVSAMPLLL IMGLLLLIRN CESSSSIPGP GYCLGIGPLI 70 80 90 100 110 120 SHGRFLWMGI GSACNYYNKM YGEFMRVWIS GEETLIISKS SSMVHVMKHS NYISRFGSKR 130 140 150 160 170 180 GLQCIGMHEN GIIFNNNPSL WRTVRPFFMK ALTGPGLIRM VEVCVESIKQ HLDRLGDVTD 190 200 210 220 230 240 NSGYVDVVTL MRHIMLDTSN TLFLGIPLDE SSIVKKIQGY FNAWQALLIK PNIFFKISWL 250 260 270 280 290 300 YRKYERSVKD LKDEIEILVE KKRQKVSSAE KLEDCMDFAT DLIFAERRGD LTKENVNQCI 310 320 330 340 350 360 LEMLIAAPDT MSVTLYVMLL LIAEYPEVET AILKEIHTVV GDRDIRIGDV QNLKVVENFI 370 380 390 400 410 420 NESLRYQPVV DLVMRRALED DVIDGYPVKK GTNIILNIGR MHRLEYFPKP NEFTLENFEK 430 440 450 460 470 480 NVPYRYFQPF GFGPRSCAGK YIAMVMMKVV LVTLLKRFHV KTLQKRCIEN MPKNNDLSLH 490 500 LDEDSPIVEI IFRHIFNTPF LQCLYISL

References

[1]	"Aromatase cytochrome P450 in rat ovarian granulosa cells before and after luteinization: adenosine 3',5'-monophosphate-dependent and independent regulation. Cloning and sequencing of rat aromatase cDNA and 5' genomic DNA." Hickey G.J., Krasnow J.S., Beattie W.G., Richards J.S. Mol. Endocrinol. 4:3-12(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.
[2]	"The structure of cDNA clones encoding the aromatase P-450 isolated from a rat Leydig cell tumor line demonstrates differential processing of aromatase mRNA in rat ovary and a neoplastic cell line." Lephart E.D., Peterson K.G., Noble J.F., George F.W., McPhaul M.J. Mol. Cell. Endocrinol. 70:31-40(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M33986 mRNA. Translation: AAA41044.1.
IPI	IPI00207135.
PIR	A36121.
UniGene	Rn.21402.
3D structure databases
ProteinModelPortal	P22443.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000000212.
Proteomic databases
PaxDb	P22443.
PRIDE	P22443.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
RGD	2457. Cyp19a1.
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000111912.
HOVERGEN	HBG050750.
OrthoDB	EOG4ZCT4K.
Gene expression databases
ArrayExpress	P22443.
Genevestigator	P22443.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P22443.
ChEMBL	CHEMBL3859.

Entry information

Entry name

CP19A_RAT

Accession

Primary (citable) accession number: P22443

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	April 3, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program