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Protein

Pyruvate dehydrogenase protein X component

Gene

PDHX

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex.

GO - Molecular functioni

Enzyme and pathway databases

ReactomeiR-BTA-204174 Regulation of pyruvate dehydrogenase (PDH) complex
R-BTA-389661 Glyoxylate metabolism and glycine degradation
R-BTA-5362517 Signaling by Retinoic Acid
R-BTA-70268 Pyruvate metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase protein X component
Alternative name(s):
Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex
E3-binding protein
Short name:
E3BP
proX
Gene namesi
Name:PDHX
Synonyms:PDX1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 15

Organism-specific databases

VGNCiVGNC:32695 PDHX

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 53MitochondrionBy similarityAdd BLAST53
ChainiPRO_000016230154 – 501Pyruvate dehydrogenase protein X componentAdd BLAST448

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei97N6-lipoyllysinePROSITE-ProRule annotationBy similarity1
Modified residuei194N6-acetyllysineBy similarity1
Modified residuei394N6-succinyllysineBy similarity1

Post-translational modificationi

Delipoylated at Lys-97 by SIRT4, delipoylation decreases the PHD complex activity.By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP22439
PeptideAtlasiP22439
PRIDEiP22439

Expressioni

Gene expression databases

BgeeiENSBTAG00000018261

Interactioni

Subunit structurei

Part of the inner core of the multimeric pyruvate dehydrogenase complex that is composed of about 48 DLAT and 12 PDHX molecules (PubMed:20361979). This core binds multiple copies of pyruvate dehydrogenase (subunits PDH1A and PDHB, E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3) (By similarity). Interacts with SIRT4 (By similarity). Interacts with DLD (By similarity).By similarity1 Publication

Protein-protein interaction databases

CORUMiP22439
STRINGi9913.ENSBTAP00000024307

Structurei

3D structure databases

ProteinModelPortaliP22439
SMRiP22439
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini56 – 132Lipoyl-bindingPROSITE-ProRule annotationAdd BLAST77
Domaini183 – 220Peripheral subunit-binding (PSBD)PROSITE-ProRule annotationAdd BLAST38

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi149 – 170Pro-richAdd BLAST22

Sequence similaritiesi

Belongs to the 2-oxoacid dehydrogenase family.Curated

Keywords - Domaini

Lipoyl, Transit peptide

Phylogenomic databases

eggNOGiKOG0557 Eukaryota
COG0508 LUCA
GeneTreeiENSGT00890000139393
HOGENOMiHOG000281566
HOVERGENiHBG005063
InParanoidiP22439
KOiK13997
OMAiSWRLGCD
OrthoDBiEOG091G0CAV
TreeFamiTF332256

Family and domain databases

Gene3Di3.30.559.10, 1 hit
4.10.320.10, 1 hit
InterProiView protein in InterPro
IPR003016 2-oxoA_DH_lipoyl-BS
IPR001078 2-oxoacid_DH_actylTfrase
IPR000089 Biotin_lipoyl
IPR023213 CAT-like_dom_sf
IPR036625 E3-bd_dom_sf
IPR004167 PSBD
IPR011053 Single_hybrid_motif
PfamiView protein in Pfam
PF00198 2-oxoacid_dh, 1 hit
PF00364 Biotin_lipoyl, 1 hit
PF02817 E3_binding, 1 hit
SUPFAMiSSF47005 SSF47005, 1 hit
SSF51230 SSF51230, 1 hit
PROSITEiView protein in PROSITE
PS50968 BIOTINYL_LIPOYL, 1 hit
PS00189 LIPOYL, 1 hit
PS51826 PSBD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22439-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASWRLGCD PRLLRCLLGF GSRRSPELVK GAARWSVGRG ASWRWFHSTQ
60 70 80 90 100
WLRADPIKIL MPSLSPTMEE GNIVKWLKKE GEAVSAGDAL CEIETDKAVV
110 120 130 140 150
TLDASDDGIL AKIVVAEGSK NIRLGSLIGL LVEEGEDWKH VEIPKDTGPP
160 170 180 190 200
PPAAKPSVPP PSAEPQIATP VKKEHPPGKV QFRLSPAARN ILEKHALDAN
210 220 230 240 250
QGTATGPRGI FTKEDALKLV QLKQTGKITE PRPTAALPTT PAAPLPPQAA
260 270 280 290 300
ATASYPRPMI PPVSTPGQPN VEGTFTEIPA SNIRRVIAKR LTESKSTIPH
310 320 330 340 350
AYATTDCDLG AVLTARQNLV RDDIKVSVND FIIKAAAVTL KQMPNVNASW
360 370 380 390 400
DGEGAKQLPS IDISVAVATD RGLITPVIKD AAAKGLQEIA DSVKALSKKA
410 420 430 440 450
RDGKLLPEEY QGGSFSISNL GMFGIDEFTA VINPPQACIL AVGRFRPVLK
460 470 480 490 500
LTQDEEGNAQ LQQRQLITVT MSSDSRVVDD ELATRFLESF KANLENPLRL

A
Length:501
Mass (Da):53,886
Last modified:June 16, 2009 - v3
Checksum:iA52F1813706AAD5B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55D → G AA sequence (PubMed:2914903).Curated1
Sequence conflicti78K → I AA sequence (PubMed:2914903).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti66P → G1 Publication1
Natural varianti72N → G1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC120413 mRNA Translation: AAI20414.1
PIRiA32040
RefSeqiNP_001069219.1, NM_001075751.1
UniGeneiBt.6683

Genome annotation databases

EnsembliENSBTAT00000024307; ENSBTAP00000024307; ENSBTAG00000018261
GeneIDi517402
KEGGibta:517402

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiODPX_BOVIN
AccessioniPrimary (citable) accession number: P22439
Secondary accession number(s): Q0P576
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: June 16, 2009
Last modified: March 28, 2018
This is version 118 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health