P22438 (SYMM_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase, mitochondrial
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	MSM1
Ordered Locus Names:	YGR171C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
Subcellular location	Mitochondrion matrix.
Miscellaneous	Present with 3120 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from mutant phenotype Ref.1. Source: SGD
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from mutant phenotype Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Methionine--tRNA ligase, mitochondrial		PRO_0000139272
Regions
	Motif	20 – 32	13	"HIGH" region
	Motif	341 – 345	5	"KMSKS" region
Sites
	Binding site	344	1	ATP By similarity
Experimental info
	Sequence conflict	14	1	V → I in CAA32778. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P22438 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: E105AD353D5C82B3	FASTA	575	66,734
	10 20 30 40 50 60 MQCRSIVHRL YSKVSHVTTP IFYPNAKPHL GHLYSSLLSD VYHRWQLFKG NLSFFTTGTD 70 80 90 100 110 120 EHGLKIQCAS ESNGFDQPKK FVDKLYPEFV QLDKIYGINY TRFIRTTDPD HIENVMKLWE 130 140 150 160 170 180 LCLKNGYIYM GEHKGWYSIS DETFYPESKV IKDPKNDGKY LNTESKNEVV YQSETNYFFR 190 200 210 220 230 240 LSLFNKKIVD HIRKNPDFIF PASKRDQILK ELETGGTLPD LSISRPSARL KWGIPTPNDP 250 260 270 280 290 300 SQKVYVWFDA LCNYLSSIGG IPSILSNATE VVSRHYSDKS NVKGQLLIPY PKEVQRNTIH 310 320 330 340 350 360 VIGKDIAKFH TVYWPSFLLA AGLPLPRQIV VHGHWLCNGM KMSKSLGNVV DPIDMARYYG 370 380 390 400 410 420 ADIVRWFLLE NSKLEEDGDF QEAKLYETRE LLVSKWGNLI NRCCGSKFNI ERAVMKFSDK 430 440 450 460 470 480 ANFQFQEIFQ NEPIVSERIE NLAKLLNKSQ EVFDEKIAIF QYPQLLRHVW SIINDANTLV 490 500 510 520 530 540 QNSKPWEREL DQQDNIIFLA METSRILSIL CQSIIPSLSQ SFLDRIDVSK EKRTINYARL 550 560 570 GSDKTYGKQS NKKGREVPLK KIPFRLQEEQ TNMRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of MSM1, the structural gene for yeast mitochondrial methionyl-tRNA synthetase." Tzagoloff A., Vambutas A., Akai A. Eur. J. Biochem. 179:365-371(1989) [PubMed: 2645139] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: D273-10B/A1.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14629 Genomic DNA. Translation: CAA32778.1. Z72956 Genomic DNA. Translation: CAA97197.1. BK006941 Genomic DNA. Translation: DAA08266.1.
PIR	SYBYMM. S64485.
RefSeq	NP_011687.1. NM_001181300.1.
3D structure databases
ProteinModelPortal	P22438.
SMR	P22438. Positions 12-533.
ModBase	Search...
Protein-protein interaction databases
IntAct	P22438. 2 interactions.
MINT	MINT-2783147.
STRING	P22438.
Proteomic databases
PeptideAtlas	P22438.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR171C; YGR171C; YGR171C.
GeneID	853081.
KEGG	sce:YGR171C.
NMPDR	fig|4932.3.peg.2813.
Organism-specific databases
CYGD	YGR171c.
SGD	S000003403. MSM1.
Phylogenomic databases
eggNOG	fuNOG04628.
GeneTree	EFGT00050000003465.
HOGENOM	HBG721667.
OMA	FWGVLKS.
OrthoDB	EOG4TB7KT.
Gene expression databases
ArrayExpress	P22438.
Genevestigator	P22438.
GermOnline	YGR171C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR014758. Met-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01874.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. MetG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973050.

Entry information

Entry name

SYMM_YEAST

Accession

Primary (citable) accession number: P22438
Secondary accession number(s): D6VUV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program