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Protein

Methionine--tRNA ligase, mitochondrial

Gene

MSM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 3120 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei344ATPBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • methionine-tRNA ligase activity Source: SGD

GO - Biological processi

  • methionyl-tRNA aminoacylation Source: SGD

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30867-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase, mitochondrial (EC:6.1.1.10)
Alternative name(s):
Methionyl-tRNA synthetase
Short name:
MetRS
Gene namesi
Name:MSM1
Ordered Locus Names:YGR171C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR171C
SGDiS000003403 MSM1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001392721 – 575Methionine--tRNA ligase, mitochondrialAdd BLAST575

Proteomic databases

MaxQBiP22438
PaxDbiP22438
PRIDEiP22438

Interactioni

Protein-protein interaction databases

BioGridi33423, 57 interactors
IntActiP22438, 2 interactors
MINTiP22438
STRINGi4932.YGR171C

Structurei

3D structure databases

ProteinModelPortaliP22438
SMRiP22438
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi20 – 32"HIGH" regionAdd BLAST13
Motifi341 – 345"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000075136
HOGENOMiHOG000200401
InParanoidiP22438
KOiK01874
OMAiSDECFYP
OrthoDBiEOG092C3QMG

Family and domain databases

CDDicd00814 MetRS_core, 1 hit
Gene3Di3.40.50.620, 2 hits
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR014758 Met-tRNA_synth
IPR015413 Methionyl/Leucyl_tRNA_Synth
IPR033911 MetRS_core
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
PfamiView protein in Pfam
PF09334 tRNA-synt_1g, 1 hit
PRINTSiPR01041 TRNASYNTHMET
SUPFAMiSSF47323 SSF47323, 2 hits
TIGRFAMsiTIGR00398 metG, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

P22438-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQCRSIVHRL YSKVSHVTTP IFYPNAKPHL GHLYSSLLSD VYHRWQLFKG
60 70 80 90 100
NLSFFTTGTD EHGLKIQCAS ESNGFDQPKK FVDKLYPEFV QLDKIYGINY
110 120 130 140 150
TRFIRTTDPD HIENVMKLWE LCLKNGYIYM GEHKGWYSIS DETFYPESKV
160 170 180 190 200
IKDPKNDGKY LNTESKNEVV YQSETNYFFR LSLFNKKIVD HIRKNPDFIF
210 220 230 240 250
PASKRDQILK ELETGGTLPD LSISRPSARL KWGIPTPNDP SQKVYVWFDA
260 270 280 290 300
LCNYLSSIGG IPSILSNATE VVSRHYSDKS NVKGQLLIPY PKEVQRNTIH
310 320 330 340 350
VIGKDIAKFH TVYWPSFLLA AGLPLPRQIV VHGHWLCNGM KMSKSLGNVV
360 370 380 390 400
DPIDMARYYG ADIVRWFLLE NSKLEEDGDF QEAKLYETRE LLVSKWGNLI
410 420 430 440 450
NRCCGSKFNI ERAVMKFSDK ANFQFQEIFQ NEPIVSERIE NLAKLLNKSQ
460 470 480 490 500
EVFDEKIAIF QYPQLLRHVW SIINDANTLV QNSKPWEREL DQQDNIIFLA
510 520 530 540 550
METSRILSIL CQSIIPSLSQ SFLDRIDVSK EKRTINYARL GSDKTYGKQS
560 570
NKKGREVPLK KIPFRLQEEQ TNMRS
Length:575
Mass (Da):66,734
Last modified:October 1, 1996 - v2
Checksum:iE105AD353D5C82B3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14V → I in CAA32778 (PubMed:2645139).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14629 Genomic DNA Translation: CAA32778.1
Z72956 Genomic DNA Translation: CAA97197.1
BK006941 Genomic DNA Translation: DAA08266.1
PIRiS64485 SYBYMM
RefSeqiNP_011687.1, NM_001181300.1

Genome annotation databases

EnsemblFungiiYGR171C; YGR171C; YGR171C
GeneIDi853081
KEGGisce:YGR171C

Similar proteinsi

Entry informationi

Entry nameiSYMM_YEAST
AccessioniPrimary (citable) accession number: P22438
Secondary accession number(s): D6VUV5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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