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P22437 (PGH1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prostaglandin G/H synthase 1
EC=1.14.99.1
Alternative name(s):
Cyclooxygenase-1
Short name=COX-1
Prostaglandin H2 synthase 1
Short name=PGH synthase 1
Short name=PGHS-1
Short name=PHS 1
Prostaglandin-endoperoxide synthase 1
Gene names
Name:Ptgs1
Synonyms:Cox-1, Cox1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length602 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.

Catalytic activity

Arachidonate + AH2 + 2 O2 = prostaglandin H2 + A + H2O.

Cofactor

Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity.

Pathway

Lipid metabolism; prostaglandin biosynthesis.

Subunit structure

Homodimer.

Subcellular location

Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.

Miscellaneous

This enzyme acts both as a dioxygenase and as a peroxidase.

This enzyme is the target of nonsteroidal anti-inflammatory drugs such as aspirin.

Sequence similarities

Belongs to the prostaglandin G/H synthase family.

Contains 1 EGF-like domain.

Ontologies

Keywords
   Biological processFatty acid biosynthesis
Fatty acid metabolism
Lipid biosynthesis
Lipid metabolism
Prostaglandin biosynthesis
Prostaglandin metabolism
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   DomainEGF-like domain
Signal
   LigandHeme
Iron
Metal-binding
   Molecular functionDioxygenase
Oxidoreductase
Peroxidase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcyclooxygenase pathway

Inferred from electronic annotation. Source: Compara

keratinocyte differentiation

Non-traceable author statement PubMed 12067981. Source: UniProtKB

prostaglandin biosynthetic process

Inferred from mutant phenotype PubMed 12093889. Source: MGI

regulation of blood pressure

Inferred from mutant phenotype PubMed 12093889. Source: MGI

regulation of cell proliferation

Inferred from genetic interaction PubMed 10987272. Source: MGI

response to oxidative stress

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasmic part

Inferred from direct assay PubMed 12355421. Source: MGI

endoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

photoreceptor outer segment

Inferred from direct assay PubMed 12355421. Source: MGI

   Molecular_functionheme binding

Inferred from electronic annotation. Source: InterPro

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen

Inferred from electronic annotation. Source: UniProtKB-KW

peroxidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

prostaglandin-endoperoxide synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626
Chain27 – 602576Prostaglandin G/H synthase 1
PRO_0000023869

Regions

Domain34 – 7239EGF-like

Sites

Active site2091Proton acceptor By similarity
Active site3871For cyclooxygenase activity By similarity
Metal binding3901Iron (heme axial ligand) By similarity
Site5321Aspirin-acetylated serine

Amino acid modifications

Glycosylation701N-linked (GlcNAc...) Potential
Glycosylation1061N-linked (GlcNAc...) Potential
Glycosylation1461N-linked (GlcNAc...) Potential
Disulfide bond38 ↔ 49 By similarity
Disulfide bond39 ↔ 161 By similarity
Disulfide bond43 ↔ 59 By similarity
Disulfide bond61 ↔ 71 By similarity
Disulfide bond571 ↔ 577 By similarity

Sequences

Sequence LengthMass (Da)Tools
P22437 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 634C0E602045C3A0

FASTA60269,042
        10         20         30         40         50         60 
MSRRSLSLWF PLLLLLLLPP TPSVLLADPG VPSPVNPCCY YPCQNQGVCV RFGLDNYQCD 

        70         80         90        100        110        120 
CTRTGYSGPN CTIPEIWTWL RNSLRPSPSF THFLLTHGYW LWEFVNATFI REVLMRLVLT 

       130        140        150        160        170        180 
VRSNLIPSPP TYNSAHDYIS WESFSNVSYY TRILPSVPKD CPTPMGTKGK KQLPDVQLLA 

       190        200        210        220        230        240 
QQLLLRREFI PAPQGTNILF AFFAQHFTHQ FFKTSGKMGP GFTKALGHGV DLGHIYGDNL 

       250        260        270        280        290        300 
ERQYHLRLFK DGKLKYQVLD GEVYPPSVEQ ASVLMRYPPG VPPERQMAVG QEVFGLLPGL 

       310        320        330        340        350        360 
MLFSTIWLRE HNRVCDLLKE EHPTWDDEQL FQTTRLILIG ETIKIVIEEY VQHLSGYFLQ 

       370        380        390        400        410        420 
LKFDPELLFR AQFQYRNRIA MEFNHLYHWH PLMPNSFQVG SQEYSYEQFL FNTSMLVDYG 

       430        440        450        460        470        480 
VEALVDAFSR QRAGRIGGGR NFDYHVLHVA VDVIKESREM RLQPFNEYRK RFGLKPYTSF 

       490        500        510        520        530        540 
QELTGEKEMA AELEELYGDI DALEFYPGLL LEKCQPNSIF GESMIEMGAP FSLKGLLGNP 

       550        560        570        580        590        600 
ICSPEYWKPS TFGGDVGFNL VNTASLKKLV CLNTKTCPYV SFRVPDYPGD DGSVLVRRST 


EL

« Hide

References

« Hide 'large scale' references
[1]"The aspirin and heme-binding sites of ovine and murine prostaglandin endoperoxide synthases."
Dewitt D.L., El-Harith E.A., Kraemer S.A., Andrews M.J., Yao E.F., Armstrong R.L., Smith W.L.
J. Biol. Chem. 265:5192-5198(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M34141 mRNA. Translation: AAA39913.1.
BC005573 mRNA. Translation: AAH05573.1.
IPIIPI00128389.
PIRA35564.
RefSeqNP_032995.1. NM_008969.3.
UniGeneMm.275434.

3D structure databases

ProteinModelPortalP22437.
SMRP22437. Positions 34-586.
ModBaseSearch...

Protein family/group databases

PeroxiBase3361. MmPGHS01.

PTM databases

PhosphoSiteP22437.

Proteomic databases

PaxDbP22437.
PRIDEP22437.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000062069; ENSMUSP00000059977; ENSMUSG00000047250.
GeneID19224.
KEGGmmu:19224.

Organism-specific databases

CTD5742.
MGIMGI:97797. Ptgs1.

Phylogenomic databases

eggNOGNOG39991.
HOGENOMHOG000013149.
HOVERGENHBG000366.
InParanoidP22437.
KOK00509.
OMAFKTSGKM.
OrthoDBEOG402WRZ.

Enzyme and pathway databases

UniPathwayUPA00662.

Gene expression databases

ArrayExpressP22437.
BgeeP22437.
CleanExMM_PTGS1.
GenevestigatorP22437.
GermOnlineENSMUSG00000047250. Mus musculus.

Family and domain databases

Gene3D1.10.640.10. 1 hit.
InterProIPR000742. EG-like_dom.
IPR010255. Haem_peroxidase.
IPR002007. Haem_peroxidase_animal.
IPR019791. Haem_peroxidase_animal_subgr.
[Graphical view]
PfamPF03098. An_peroxidase. 2 hits.
[Graphical view]
PRINTSPR00457. ANPEROXIDASE.
SMARTSM00181. EGF. 1 hit.
[Graphical view]
SUPFAMSSF48113. Peroxidase_super. 1 hit.
PROSITEPS00022. EGF_1. False negative.
PS01186. EGF_2. False negative.
PS50026. EGF_3. 1 hit.
PS50292. PEROXIDASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBP22437.
ChEMBLCHEMBL2649.
NextBio296008.
SOURCESearch...

Entry information

Entry namePGH1_MOUSE
AccessionPrimary (citable) accession number: P22437
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: April 3, 2013
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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