Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P22435

- NCAP_I68A4

UniProt

P22435 - NCAP_I68A4

Protein

Nucleoprotein

Gene

NP

Organism
Influenza A virus (strain A/Hong Kong/1/1968 H3N2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 2 (21 Aug 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus By similarity.By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW
    2. structural molecule activity Source: InterPro

    GO - Biological processi

    1. viral entry into host cell Source: UniProtKB-KW
    2. viral penetration into host nucleus Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Biological processi

    Host-virus interaction, Viral penetration into host nucleus, Virus entry into host cell

    Keywords - Ligandi

    RNA-binding, Viral nucleoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleoprotein
    Alternative name(s):
    Nucleocapsid protein
    Short name:
    Protein N
    Gene namesi
    Name:NP
    OrganismiInfluenza A virus (strain A/Hong Kong/1/1968 H3N2)
    Taxonomic identifieri506350 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    Cetacea (whales) [TaxID: 9721]
    Homo sapiens (Human) [TaxID: 9606]
    Phocidae (true seals) [TaxID: 9709]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000008244: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. helical viral capsid Source: UniProtKB-KW
    2. host cell nucleus Source: UniProtKB-SubCell
    3. ribonucleoprotein complex Source: UniProtKB-KW
    4. viral nucleocapsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Helical capsid protein, Host nucleus, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 498498NucleoproteinPRO_0000079064Add
    BLAST

    Post-translational modificationi

    Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction By similarity.By similarity

    Interactioni

    Subunit structurei

    Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP22435.
    SMRiP22435. Positions 22-496.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1 – 1818Unconventional nuclear localization signalBy similarityAdd
    BLAST
    Motifi198 – 21619Bipartite nuclear localization signalBy similarityAdd
    BLAST

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR002141. Flu_NP.
    [Graphical view]
    PfamiPF00506. Flu_NP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P22435-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASQGTKRSY EQMETDGERQ NATEIRASVG KMIDGIGRFY IQMCTELKLS    50
    DYEGRLIQNS LTIERMVLSA FDERRNKYLE EHPSAGKDPK KTGGPIYKRV 100
    DGKWMRELVL YDKEEIRRIW RQANNGDDAT AGLTHMMIWH SNLNDTTYQR 150
    TRALVRTGMD PRMCSLMQGS TLPRRSGAAG AAVKGVGTMV MELIRMIKRG 200
    INDRNFWRGE NGRKTRSAYE RMCNILKGKF QTAAQRAMMD QVRESRNPGN 250
    AEIEDLIFLA RSALILRGSV AHKSCLPACV YGPAVASGYD FEKEGYSLVG 300
    IDPFKLLQNS QVYSLIRPNE NPAHKSQLVW MACNSAAFED LRVLSFIRGT 350
    KVSPRGKLST RGVQIASNEN MDAMESSTLE LRSRYWAIRT RSGGNTNQQR 400
    ASAGQISVQP AFSVQRNLPF DKPTIMAAFT GNTEGRTSDM RAEIIRMMEG 450
    AKPEEMSFQG RGVFELSDEK AANPIVPSFD MSNEGSYFFG DNAEEYDN 498
    Length:498
    Mass (Da):56,026
    Last modified:August 21, 2007 - v2
    Checksum:iF0AA832B97BC75CC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti311 – 3111Q → H in CAA33899. (PubMed:2780295)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti34 – 341D → N in strain: Isolate MA12, Isolate MA20 and Isolate MA20c.
    Natural varianti480 – 4801D → N in strain: Isolate MA20c.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15890 Genomic RNA. Translation: CAA33899.1.
    AF348180 Genomic RNA. Translation: AAK51722.1.
    AF348181 Genomic RNA. Translation: AAK51723.1.
    AF348182 Genomic RNA. Translation: AAK51724.1.
    AF348183 Genomic RNA. Translation: AAK51725.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15890 Genomic RNA. Translation: CAA33899.1 .
    AF348180 Genomic RNA. Translation: AAK51722.1 .
    AF348181 Genomic RNA. Translation: AAK51723.1 .
    AF348182 Genomic RNA. Translation: AAK51724.1 .
    AF348183 Genomic RNA. Translation: AAK51725.1 .

    3D structure databases

    ProteinModelPortali P22435.
    SMRi P22435. Positions 22-496.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR002141. Flu_NP.
    [Graphical view ]
    Pfami PF00506. Flu_NP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the nucleoprotein (NP) gene of the influenza A virus reassortant 81/HO, carrying the NP originally derived from A/Hong Kong/1/68 (H3N2)."
      Reinhardt U., Mandler J., Scholtissek C.
      Nucleic Acids Res. 17:6721-6721(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Pattern of mutation in the genome of influenza A virus on adaptation to increased virulence in the mouse lung: identification of functional themes."
      Brown E.G., Liu H., Kit L.C., Baird S., Nesrallah M.
      Proc. Natl. Acad. Sci. U.S.A. 98:6883-6888(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate MA12, Isolate MA20 and Isolate MA20c.

    Entry informationi

    Entry nameiNCAP_I68A4
    AccessioniPrimary (citable) accession number: P22435
    Secondary accession number(s): Q91MA3
    , Q91MA4, Q91MA5, Q91MA6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 58 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3