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P22435

- NCAP_I68A4

UniProt

P22435 - NCAP_I68A4

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Protein

Nucleoprotein

Gene

NP

Organism
Influenza A virus (strain A/Hong Kong/1/1968 H3N2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus (By similarity).By similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural molecule activity Source: InterPro

GO - Biological processi

  1. viral entry into host cell Source: UniProtKB-KW
  2. viral penetration into host nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Host-virus interaction, Viral penetration into host nucleus, Virus entry into host cell

Keywords - Ligandi

RNA-binding, Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoprotein
Alternative name(s):
Nucleocapsid protein
Short name:
Protein N
Gene namesi
Name:NP
OrganismiInfluenza A virus (strain A/Hong Kong/1/1968 H3N2)
Taxonomic identifieri506350 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Cetacea (whales) [TaxID: 9721]
Homo sapiens (Human) [TaxID: 9606]
Phocidae (true seals) [TaxID: 9709]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000008244: Genome

Subcellular locationi

GO - Cellular componenti

  1. helical viral capsid Source: UniProtKB-KW
  2. host cell nucleus Source: UniProtKB-KW
  3. ribonucleoprotein complex Source: UniProtKB-KW
  4. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498NucleoproteinPRO_0000079064Add
BLAST

Post-translational modificationi

Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction (By similarity).By similarity

Interactioni

Subunit structurei

Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP22435.
SMRiP22435. Positions 22-496.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1 – 1818Unconventional nuclear localization signalBy similarityAdd
BLAST
Motifi198 – 21619Bipartite nuclear localization signalBy similarityAdd
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR002141. Flu_NP.
[Graphical view]
PfamiPF00506. Flu_NP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22435-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASQGTKRSY EQMETDGERQ NATEIRASVG KMIDGIGRFY IQMCTELKLS
60 70 80 90 100
DYEGRLIQNS LTIERMVLSA FDERRNKYLE EHPSAGKDPK KTGGPIYKRV
110 120 130 140 150
DGKWMRELVL YDKEEIRRIW RQANNGDDAT AGLTHMMIWH SNLNDTTYQR
160 170 180 190 200
TRALVRTGMD PRMCSLMQGS TLPRRSGAAG AAVKGVGTMV MELIRMIKRG
210 220 230 240 250
INDRNFWRGE NGRKTRSAYE RMCNILKGKF QTAAQRAMMD QVRESRNPGN
260 270 280 290 300
AEIEDLIFLA RSALILRGSV AHKSCLPACV YGPAVASGYD FEKEGYSLVG
310 320 330 340 350
IDPFKLLQNS QVYSLIRPNE NPAHKSQLVW MACNSAAFED LRVLSFIRGT
360 370 380 390 400
KVSPRGKLST RGVQIASNEN MDAMESSTLE LRSRYWAIRT RSGGNTNQQR
410 420 430 440 450
ASAGQISVQP AFSVQRNLPF DKPTIMAAFT GNTEGRTSDM RAEIIRMMEG
460 470 480 490
AKPEEMSFQG RGVFELSDEK AANPIVPSFD MSNEGSYFFG DNAEEYDN
Length:498
Mass (Da):56,026
Last modified:August 21, 2007 - v2
Checksum:iF0AA832B97BC75CC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti311 – 3111Q → H in CAA33899. (PubMed:2780295)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti34 – 341D → N in strain: Isolate MA12, Isolate MA20 and Isolate MA20c.
Natural varianti480 – 4801D → N in strain: Isolate MA20c.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15890 Genomic RNA. Translation: CAA33899.1.
AF348180 Genomic RNA. Translation: AAK51722.1.
AF348181 Genomic RNA. Translation: AAK51723.1.
AF348182 Genomic RNA. Translation: AAK51724.1.
AF348183 Genomic RNA. Translation: AAK51725.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15890 Genomic RNA. Translation: CAA33899.1 .
AF348180 Genomic RNA. Translation: AAK51722.1 .
AF348181 Genomic RNA. Translation: AAK51723.1 .
AF348182 Genomic RNA. Translation: AAK51724.1 .
AF348183 Genomic RNA. Translation: AAK51725.1 .

3D structure databases

ProteinModelPortali P22435.
SMRi P22435. Positions 22-496.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002141. Flu_NP.
[Graphical view ]
Pfami PF00506. Flu_NP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of the nucleoprotein (NP) gene of the influenza A virus reassortant 81/HO, carrying the NP originally derived from A/Hong Kong/1/68 (H3N2)."
    Reinhardt U., Mandler J., Scholtissek C.
    Nucleic Acids Res. 17:6721-6721(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Pattern of mutation in the genome of influenza A virus on adaptation to increased virulence in the mouse lung: identification of functional themes."
    Brown E.G., Liu H., Kit L.C., Baird S., Nesrallah M.
    Proc. Natl. Acad. Sci. U.S.A. 98:6883-6888(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate MA12, Isolate MA20 and Isolate MA20c.

Entry informationi

Entry nameiNCAP_I68A4
AccessioniPrimary (citable) accession number: P22435
Secondary accession number(s): Q91MA3
, Q91MA4, Q91MA5, Q91MA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 21, 2007
Last modified: October 29, 2014
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3