P22434 (PDE1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3',5'-cyclic-nucleotide phosphodiesterase 1
Short name=PDEase 1
EC=3.1.4.17

Alternative name(s):
3':5'-CNP
Low-affinity cAMP phosphodiesterase

Gene names

Name:	PDE1
Ordered Locus Names:	YGL248W
ORF Names:	NRB369

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Controls the level of cAMP in yeast cells, together with the high-affinity cAMP phosphodiesterase (PDE2).
Catalytic activity	Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.
Miscellaneous	Present with 1400 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the cAMP phosphodiesterase class-II family.

Ontologies

Keywords
Ligand	cAMP
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cAMP catabolic process Inferred from electronic annotation. Source: InterPro cAMP-mediated signaling Inferred from mutant phenotype PubMed 9880329. Source: SGD
Molecular_function	3',5'-cyclic-AMP phosphodiesterase activity Inferred from mutant phenotype Ref.1PubMed 6300049. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	3',5'-cyclic-nucleotide phosphodiesterase 1		PRO_0000206791
Amino acid modifications
	Modified residue	311	1	Phosphotyrosine Ref.6
	Modified residue	313	1	Phosphoserine Ref.6
	Modified residue	314	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	94	1	L → F in AAA34896. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P22434 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 47B752477E99BA88	FASTA	369	42,016
	10 20 30 40 50 60 MVVFEITILG ANGGPTEYGT QCFILKPART EDPELIAVDG GAGMYQLREM LVQGRNENEG 70 80 90 100 110 120 DDELVPSFYE HDREPIEFFI DSKLNIQKGL SKSLLQSLKR QGEHFESANT MKKTYEVFQG 130 140 150 160 170 180 ITDYYITHPH LDHISGLVVN SPSIYEQENS KKKTIWGLPH TIDVLQKHVF NDLIWPDLTA 190 200 210 220 230 240 ERSRKLKLKC LNPKEVQKCT IFPWDVIPFK VHHGIGVKTG APVYSTFYIF RDRKSKDCII 250 260 270 280 290 300 VCGDVEQDRR ESEESLLEEF WSYVAENIPL VHLKGILVEC SCPLSSKPEQ LYGHLSPIYL 310 320 330 340 350 360 INELSNLNTL YNSSKGLSGL NVIVTHVKST PAKRDPRLTI LEELRFLAEE RNLGDLRISI ALEGHTLFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the low-affinity cyclic AMP phosphodiesterase gene of Saccharomyces cerevisiae." Nikawa J., Sass P., Wigler M. Mol. Cell. Biol. 7:3629-3636(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence of a 39,411 bp DNA fragment covering the left end of chromosome VII of Saccharomyces cerevisiae." Coissac E., Maillier E., Robineau S., Netter P. Yeast 12:1555-1562(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-311; SER-313 AND SER-314, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M17781 Genomic DNA. Translation: AAA34896.1. X94357 Genomic DNA. Translation: CAA64139.1. Z72770 Genomic DNA. Translation: CAA96968.1. BK006941 Genomic DNA. Translation: DAA07871.1.
PIR	ESBYPC. S61613.
RefSeq	NP_011266.1. NM_001181114.1.
3D structure databases
ProteinModelPortal	P22434.
ModBase	Search...
Protein-protein interaction databases
STRING	4932.YGL248W.
Proteomic databases
PaxDb	P22434.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL248W; YGL248W; YGL248W.
GeneID	852644.
KEGG	sce:YGL248W.
Organism-specific databases
CYGD	YGL248w.
SGD	S000003217. PDE1.
Phylogenomic databases
eggNOG	COG5212.
HOGENOM	HOG000248334.
KO	K01120.
OMA	NHVKEPI.
OrthoDB	EOG4RNFJX.
Gene expression databases
Genevestigator	P22434.
GermOnline	YGL248W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR024225. cAMP-PdiesteraseII_CS. IPR000396. Pdiesterase2. [Graphical view]
Pfam	PF02112. PDEase_II. 1 hit. [Graphical view]
PIRSF	PIRSF000962. Cyc_nuc_PDEase. 1 hit.
PRINTS	PR00388. PDIESTERASE2.
PROSITE	PS00607. PDEASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971903.

Entry information

Entry name

PDE1_YEAST

Accession

Primary (citable) accession number: P22434
Secondary accession number(s): D6VV87

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program