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Reviewed, UniProtKB/Swiss-Prot P22415 (USF1_HUMAN)

Last modified February 9, 2010. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Upstream stimulatory factor 1
Alternative name(s):
    Major late transcription factor 1
    Class B basic helix-loop-helix protein 11
      Short name=bHLHb11
Gene names
Name: USF1
Synonyms: BHLHB11, USF
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length310 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

Subunit structure

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as an homodimer or a heterodimer (USF1/USF2). Interacts with varicella-zoster virus IE62 protein. Ref.8

Subcellular location

Nucleus.

Involvement in disease

Genetic variations in USF1 are associated with combined hyperlipidemia type 1 (HYPLIP1) [MIM:602491]; also known as familial combined hyperlipidemia type 1 (FCHL1). HYPLIP1 is characterized by elevated levels of serum total cholesterol, triglycerides or both, and is observed in about 20% of individuals with premature coronary heart disease. Ref.10

Sequence similarities

Contains 1 basic helix-loop-helix (bHLH) domain.

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Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processcellular response to insulin stimulus

Inferred from direct assay. Source: UniProtKB

glucose homeostasis

Traceable author statement. Source: UniProtKB

late viral mRNA transcription Ref.1

Inferred from direct assay. Source: UniProtKB

lipid homeostasis

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of fibrinolysis

Inferred by curator. Source: UniProtKB

positive regulation of gene-specific transcription from RNA polymerase II promoter

Inferred from mutant phenotype. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter by glucose

Inferred from mutant phenotype. Source: UniProtKB

response to UV

Inferred from sequence or structural similarity. Source: UniProtKB

response to hypoxia

Inferred from mutant phenotype. Source: UniProtKB

transcription from RNA polymerase II promoter Ref.1

Inferred from direct assay. Source: UniProtKB

   Cellular componenttranscription factor complex

Inferred from direct assay. Source: UniProtKB

   Molecular functionRNA polymerase II transcription factor activity, enhancer binding Ref.1

Inferred from direct assay. Source: UniProtKB

bHLH transcription factor binding

Inferred from physical interaction. Source: UniProtKB

histone deacetylase binding

Inferred from physical interaction. Source: UniProtKB

protein heterodimerization activity

Inferred from physical interaction. Source: UniProtKB

protein homodimerization activity Ref.1

Inferred from physical interaction. Source: UniProtKB

protein kinase binding

Inferred from physical interaction. Source: UniProtKB

sequence-specific DNA binding Ref.1

Inferred from direct assay. Source: UniProtKB

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

USF2Q158531EBI-1054489,EBI-1055994

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 310310Upstream stimulatory factor 1
PRO_0000127496

Regions

Domain213 – 25543Helix-loop-helix motif
Domain271 – 29222Leucine-zipper
DNA binding200 – 21213Basic motif

Secondary structure

1 ......... 310
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P22415-1 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: BFDA91519B4B80AE

FASTA31033,538
        10         20         30         40         50         60 
MKGQQKTAET EEGTVQIQEG AVATGEDPTS VAIASIQSAA TFPDPNVKYV FRTENGGQVM 

        70         80         90        100        110        120 
YRVIQVSEGQ LDGQTEGTGA ISGYPATQSM TQAVIQGAFT SDDAVDTEGT AAETHYTYFP 

       130        140        150        160        170        180 
STAVGDGAGG TTSGSTAAVV TTQGSEALLG QATPPGTGQF FVMMSPQEVL QGGSQRSIAP 

       190        200        210        220        230        240 
RTHPYSPKSE APRTTRDEKR RAQHNEVERR RRDKINNWIV QLSKIIPDCS MESTKSGQSK 

       250        260        270        280        290        300 
GGILSKACDY IQELRQSNHR LSEELQGLDQ LQLDNDVLRQ QVEDLKNKNL LLRAQLRHHG 

       310 
LEVVIKNDSN

« Hide

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References

« Hide 'large scale' references
[1]"The adenovirus major late transcription factor USF is a member of the helix-loop-helix group of regulatory proteins and binds to DNA as a dimer."
Gregor P.D., Sawadogo M., Roeder R.G.
Genes Dev. 4:1730-1740(1990) [PubMed: 2249772] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-25.
[2]"Human USF1 genomic sequence."
Fukamizu A.
Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[4]SeattleSNPs variation discovery resource
Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed: 16710414] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[8]"Transcription factor USF, expressed during the entire phase of Varicella-zoster virus infection, interacts physically with the major viral transactivator IE62 and plays a significant role in virus replication."
Rahaus M., Desloges N., Yang M., Ruyechan W.T., Wolff M.H.
J. Gen. Virol. 84:2957-2967(2003) [PubMed: 14573800] [Abstract]
Cited for: INTERACTION WITH VARICELLA-ZOSTER VIRUS IE62 PROTEIN.
[9]"Structure and function of the b/HLH/Z domain of USF."
Ferre-D'Amare A.R., Pognonec P., Roeder R.G., Burley S.K.
EMBO J. 13:180-189(1994) [PubMed: 8306960] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 197-260.
[10]"Familial combined hyperlipidemia is associated with upstream transcription factor 1 (USF1)."
Pajukanta P., Lilja H.E., Sinsheimer J.S., Cantor R.M., Lusis A.J., Gentile M., Duan X.J., Soro-Paavonen A., Naukkarinen J., Saarela J., Laakso M., Ehnholm C., Taskinen M.-R., Peltonen L.
Nat. Genet. 36:371-376(2004) [PubMed: 14991056] [Abstract]
Cited for: INVOLVEMENT IN HYPLIP1.
+Additional computationally mapped references.

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Web resources

SeattleSNPs

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X55666 mRNA. Translation: CAA39201.1.
AB017568 Genomic DNA. Translation: BAA76541.1.
AK314876 mRNA. Translation: BAG37391.1.
AY593992 Genomic DNA. Translation: AAS89301.1.
AL591806 Genomic DNA. Translation: CAI15372.1.
CH471121 Genomic DNA. Translation: EAW52675.1.
BC035505 mRNA. Translation: AAH35505.1.
IPIIPI00026559.
PIRS13525.
RefSeqNP_009053.1.
NP_996888.1.
UniGeneHs.414880

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AN4X-ray2.90A/B197-260[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-654N.
IntActP22415. 11 interactions.
STRINGP22415.

PTM databases

PhosphoSiteP22415.

Proteomic databases

PRIDEP22415.

Genome annotation databases

EnsemblENST00000368020; ENSP00000356999; ENSG00000158773; Homo sapiens. [Genome view]
ENST00000368021; ENSP00000357000; ENSG00000158773; Homo sapiens. [Genome view]
GeneID7391.
KEGGhsa:7391.
UCSCuc001fxi.1. human.

Organism-specific databases

CTD7391.
GeneCardsGC01M159275.
H-InvDBHIX0001226.
HGNCHGNC:12593. USF1.
HPACAB001480.
MIM144250. phenotype.
191523. gene.
602491. phenotype.
PharmGKBPA37223.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG15120.
HOGENOMHBG717130.
HOVERGENP22415.
InParanoidP22415.
OMATETHYTY.
OrthoDBEOG9QJV6D.
PhylomeDBP22415.

Enzyme and pathway databases

Pathway_Interaction_DBp38alphabetadownstreampathway. Signaling mediated by p38-alpha and p38-beta.

Gene expression databases

ArrayExpressP22415.
BgeeP22415.
CleanExHS_USF1.
GenevestigatorP22415.
GermOnlineENSG00000158773. Homo sapiens.

Family and domain databases

InterProIPR001092. HLH_DNA-bd_dom.
IPR011598. HLH_DNA_bd.
[Graphical view]
Gene3DG3DSA:4.10.280.10. HLH_DNA_bd. 1 hit.
PfamPF00010. HLH. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
[Graphical view]
PROSITEPS50888. HLH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio28938.
SOURCESearch...

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Entry information

Entry nameUSF1_HUMAN
AccessionPrimary (citable) accession number: P22415
Secondary accession number(s): B2RBZ4, Q5SY46
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: February 9, 2010
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents