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P22415

- USF1_HUMAN

UniProt

P22415 - USF1_HUMAN

Protein

Upstream stimulatory factor 1

Gene

USF1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 146 (01 Oct 2014)
      Sequence version 1 (01 Aug 1991)
      Previous versions | rss
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    Functioni

    Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

    GO - Molecular functioni

    1. bHLH transcription factor binding Source: BHF-UCL
    2. double-stranded DNA binding Source: Ensembl
    3. enzyme binding Source: BHF-UCL
    4. histone deacetylase binding Source: BHF-UCL
    5. protein binding Source: IntAct
    6. protein heterodimerization activity Source: BHF-UCL
    7. protein homodimerization activity Source: BHF-UCL
    8. protein kinase binding Source: BHF-UCL
    9. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: BHF-UCL
    10. sequence-specific DNA binding Source: BHF-UCL

    GO - Biological processi

    1. carbon catabolite regulation of transcription Source: BHF-UCL
    2. cellular response to insulin stimulus Source: BHF-UCL
    3. glucose homeostasis Source: BHF-UCL
    4. glucose metabolic process Source: Ensembl
    5. late viral transcription Source: BHF-UCL
    6. lipid homeostasis Source: BHF-UCL
    7. negative regulation of fibrinolysis Source: BHF-UCL
    8. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    9. positive regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
    10. regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    11. regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
    12. response to hypoxia Source: BHF-UCL
    13. response to UV Source: BHF-UCL
    14. transcription from RNA polymerase II promoter Source: BHF-UCL

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    SignaLinkiP22415.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Upstream stimulatory factor 1
    Alternative name(s):
    Class B basic helix-loop-helix protein 11
    Short name:
    bHLHb11
    Major late transcription factor 1
    Gene namesi
    Name:USF1
    Synonyms:BHLHB11, USF
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:12593. USF1.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: BHF-UCL
    2. transcription factor complex Source: BHF-UCL

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Involvement in diseasei

    Hyperlipidemia combined 1 (HYPLIP1) [MIM:602491]: A disorder characterized by a variable pattern of elevated levels of serum total cholesterol, triglycerides or both. It is observed in a percentage of individuals with premature coronary heart disease.1 Publication
    Note: Disease susceptibility is associated with variations affecting the gene represented in this entry.

    Organism-specific databases

    MIMi144250. phenotype.
    602491. phenotype.
    PharmGKBiPA37223.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 310310Upstream stimulatory factor 1PRO_0000127496Add
    BLAST

    Proteomic databases

    MaxQBiP22415.
    PaxDbiP22415.
    PRIDEiP22415.

    PTM databases

    PhosphoSiteiP22415.

    Expressioni

    Gene expression databases

    ArrayExpressiP22415.
    BgeeiP22415.
    CleanExiHS_USF1.
    GenevestigatoriP22415.

    Organism-specific databases

    HPAiCAB001480.
    HPA036233.
    HPA036535.

    Interactioni

    Subunit structurei

    Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2). Interacts with varicella-zoster virus IE62 protein.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    KAT2BQ928315EBI-1054489,EBI-477430

    Protein-protein interaction databases

    BioGridi113237. 41 interactions.
    DIPiDIP-654N.
    IntActiP22415. 13 interactions.
    STRINGi9606.ENSP00000356999.

    Structurei

    Secondary structure

    1
    310
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi205 – 22521
    Beta strandi233 – 2364
    Turni240 – 2478
    Helixi248 – 2558
    Turni256 – 2583

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AN4X-ray2.90A/B197-260[»]
    ProteinModelPortaliP22415.
    SMRiP22415. Positions 197-260.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP22415.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini199 – 25456bHLHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni271 – 29222Leucine-zipperAdd
    BLAST

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG272240.
    HOGENOMiHOG000294174.
    HOVERGENiHBG004346.
    InParanoidiP22415.
    KOiK09106.
    OMAiHEVELTN.
    OrthoDBiEOG7GXPBW.
    PhylomeDBiP22415.
    TreeFamiTF323338.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    [Graphical view]
    PfamiPF00010. HLH. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P22415-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKGQQKTAET EEGTVQIQEG AVATGEDPTS VAIASIQSAA TFPDPNVKYV    50
    FRTENGGQVM YRVIQVSEGQ LDGQTEGTGA ISGYPATQSM TQAVIQGAFT 100
    SDDAVDTEGT AAETHYTYFP STAVGDGAGG TTSGSTAAVV TTQGSEALLG 150
    QATPPGTGQF FVMMSPQEVL QGGSQRSIAP RTHPYSPKSE APRTTRDEKR 200
    RAQHNEVERR RRDKINNWIV QLSKIIPDCS MESTKSGQSK GGILSKACDY 250
    IQELRQSNHR LSEELQGLDQ LQLDNDVLRQ QVEDLKNKNL LLRAQLRHHG 300
    LEVVIKNDSN 310
    Length:310
    Mass (Da):33,538
    Last modified:August 1, 1991 - v1
    Checksum:iBFDA91519B4B80AE
    GO
    Isoform 2 (identifier: P22415-2) [UniParc]FASTAAdd to Basket

    Also known as: usf1-bd

    The sequence of this isoform differs from the canonical sequence as follows:
         1-59: Missing.

    Show »
    Length:251
    Mass (Da):27,372
    Checksum:iAA50D545E179EFCB
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 5959Missing in isoform 2. 1 PublicationVSP_047740Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55666 mRNA. Translation: CAA39201.1.
    AB017568 Genomic DNA. Translation: BAA76541.1.
    AB098540 mRNA. Translation: BAC78384.1.
    AK314876 mRNA. Translation: BAG37391.1.
    AL832119 mRNA. No translation available.
    AY593992 Genomic DNA. Translation: AAS89301.1.
    AL591806 Genomic DNA. Translation: CAI15372.1.
    CH471121 Genomic DNA. Translation: EAW52675.1.
    BC035505 mRNA. Translation: AAH35505.1.
    CCDSiCCDS1214.1. [P22415-1]
    PIRiS13525.
    RefSeqiNP_001263302.1. NM_001276373.1. [P22415-1]
    NP_009053.1. NM_007122.4. [P22415-1]
    NP_996888.1. NM_207005.2. [P22415-2]
    UniGeneiHs.414880.

    Genome annotation databases

    EnsembliENST00000368020; ENSP00000356999; ENSG00000158773. [P22415-1]
    ENST00000368021; ENSP00000357000; ENSG00000158773. [P22415-1]
    GeneIDi7391.
    KEGGihsa:7391.
    UCSCiuc001fxi.4. human. [P22415-1]

    Polymorphism databases

    DMDMi137170.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    SeattleSNPs
    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55666 mRNA. Translation: CAA39201.1 .
    AB017568 Genomic DNA. Translation: BAA76541.1 .
    AB098540 mRNA. Translation: BAC78384.1 .
    AK314876 mRNA. Translation: BAG37391.1 .
    AL832119 mRNA. No translation available.
    AY593992 Genomic DNA. Translation: AAS89301.1 .
    AL591806 Genomic DNA. Translation: CAI15372.1 .
    CH471121 Genomic DNA. Translation: EAW52675.1 .
    BC035505 mRNA. Translation: AAH35505.1 .
    CCDSi CCDS1214.1. [P22415-1 ]
    PIRi S13525.
    RefSeqi NP_001263302.1. NM_001276373.1. [P22415-1 ]
    NP_009053.1. NM_007122.4. [P22415-1 ]
    NP_996888.1. NM_207005.2. [P22415-2 ]
    UniGenei Hs.414880.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AN4 X-ray 2.90 A/B 197-260 [» ]
    ProteinModelPortali P22415.
    SMRi P22415. Positions 197-260.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113237. 41 interactions.
    DIPi DIP-654N.
    IntActi P22415. 13 interactions.
    STRINGi 9606.ENSP00000356999.

    PTM databases

    PhosphoSitei P22415.

    Polymorphism databases

    DMDMi 137170.

    Proteomic databases

    MaxQBi P22415.
    PaxDbi P22415.
    PRIDEi P22415.

    Protocols and materials databases

    DNASUi 7391.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000368020 ; ENSP00000356999 ; ENSG00000158773 . [P22415-1 ]
    ENST00000368021 ; ENSP00000357000 ; ENSG00000158773 . [P22415-1 ]
    GeneIDi 7391.
    KEGGi hsa:7391.
    UCSCi uc001fxi.4. human. [P22415-1 ]

    Organism-specific databases

    CTDi 7391.
    GeneCardsi GC01M161009.
    HGNCi HGNC:12593. USF1.
    HPAi CAB001480.
    HPA036233.
    HPA036535.
    MIMi 144250. phenotype.
    191523. gene.
    602491. phenotype.
    neXtProti NX_P22415.
    PharmGKBi PA37223.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG272240.
    HOGENOMi HOG000294174.
    HOVERGENi HBG004346.
    InParanoidi P22415.
    KOi K09106.
    OMAi HEVELTN.
    OrthoDBi EOG7GXPBW.
    PhylomeDBi P22415.
    TreeFami TF323338.

    Enzyme and pathway databases

    SignaLinki P22415.

    Miscellaneous databases

    ChiTaRSi USF1. human.
    EvolutionaryTracei P22415.
    GeneWikii USF1.
    GenomeRNAii 7391.
    NextBioi 28938.
    PROi P22415.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P22415.
    Bgeei P22415.
    CleanExi HS_USF1.
    Genevestigatori P22415.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    [Graphical view ]
    Pfami PF00010. HLH. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The adenovirus major late transcription factor USF is a member of the helix-loop-helix group of regulatory proteins and binds to DNA as a dimer."
      Gregor P.D., Sawadogo M., Roeder R.G.
      Genes Dev. 4:1730-1740(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 1-25.
    2. "Human USF1 genomic sequence."
      Fukamizu A.
      Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Cloning and characterization of a novel splicing isoform of USF1."
      Saito T., Oishi T., Yanai K., Shimamoto Y., Fukamizu A.
      Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING.
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Testis.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Fetal kidney.
    6. SeattleSNPs variation discovery resource
      Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    7. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Kidney.
    10. "Transcription factor USF, expressed during the entire phase of Varicella-zoster virus infection, interacts physically with the major viral transactivator IE62 and plays a significant role in virus replication."
      Rahaus M., Desloges N., Yang M., Ruyechan W.T., Wolff M.H.
      J. Gen. Virol. 84:2957-2967(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH VARICELLA-ZOSTER VIRUS IE62 PROTEIN.
    11. "Structure and function of the b/HLH/Z domain of USF."
      Ferre-D'Amare A.R., Pognonec P., Roeder R.G., Burley S.K.
      EMBO J. 13:180-189(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 197-260.
    12. Cited for: INVOLVEMENT IN HYPLIP1.

    Entry informationi

    Entry nameiUSF1_HUMAN
    AccessioniPrimary (citable) accession number: P22415
    Secondary accession number(s): B2RBZ4, Q5SY46, Q7Z5Y1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 146 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3