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Protein

Upstream stimulatory factor 1

Gene

USF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

GO - Molecular functioni

  • bHLH transcription factor binding Source: BHF-UCL
  • double-stranded DNA binding Source: Ensembl
  • enzyme binding Source: BHF-UCL
  • histone deacetylase binding Source: BHF-UCL
  • identical protein binding Source: IntAct
  • protein heterodimerization activity Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • protein kinase binding Source: BHF-UCL
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: NTNU_SB
  • sequence-specific DNA binding Source: BHF-UCL
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: BHF-UCL

GO - Biological processi

  • carbon catabolite regulation of transcription Source: BHF-UCL
  • cellular response to insulin stimulus Source: BHF-UCL
  • glucose homeostasis Source: BHF-UCL
  • glucose metabolic process Source: Ensembl
  • late viral transcription Source: BHF-UCL
  • lipid homeostasis Source: BHF-UCL
  • negative regulation of fibrinolysis Source: BHF-UCL
  • positive regulation of transcription by RNA polymerase II Source: BHF-UCL
  • positive regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
  • regulation of transcription by RNA polymerase II Source: BHF-UCL
  • regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
  • response to hypoxia Source: BHF-UCL
  • response to UV Source: BHF-UCL
  • transcription by RNA polymerase II Source: BHF-UCL

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-9018519 Estrogen-dependent gene expression
SignaLinkiP22415
SIGNORiP22415

Names & Taxonomyi

Protein namesi
Recommended name:
Upstream stimulatory factor 1
Alternative name(s):
Class B basic helix-loop-helix protein 11
Short name:
bHLHb11
Major late transcription factor 1
Gene namesi
Name:USF1
Synonyms:BHLHB11, USF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000158773.14
HGNCiHGNC:12593 USF1
MIMi191523 gene
neXtProtiNX_P22415

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Hyperlipidemia combined 1 (HYPLIP1)1 Publication
Disease susceptibility is associated with variations affecting the gene represented in this entry.
Disease descriptionA disorder characterized by a variable pattern of elevated levels of serum total cholesterol, triglycerides or both. It is observed in a percentage of individuals with premature coronary heart disease.
See also OMIM:602491

Organism-specific databases

DisGeNETi7391
MalaCardsiUSF1
MIMi144250 phenotype
602491 phenotype
OpenTargetsiENSG00000158773
PharmGKBiPA37223

Polymorphism and mutation databases

DMDMi137170

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001274961 – 310Upstream stimulatory factor 1Add BLAST310

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki306Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiP22415
MaxQBiP22415
PaxDbiP22415
PeptideAtlasiP22415
PRIDEiP22415

PTM databases

iPTMnetiP22415
PhosphoSitePlusiP22415

Expressioni

Gene expression databases

BgeeiENSG00000158773
CleanExiHS_USF1
ExpressionAtlasiP22415 baseline and differential
GenevisibleiP22415 HS

Organism-specific databases

HPAiCAB001480
HPA036233
HPA036535

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2). Interacts with varicella-zoster virus IE62 protein.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • bHLH transcription factor binding Source: BHF-UCL
  • enzyme binding Source: BHF-UCL
  • histone deacetylase binding Source: BHF-UCL
  • identical protein binding Source: IntAct
  • protein heterodimerization activity Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • protein kinase binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi113237, 64 interactors
CORUMiP22415
DIPiDIP-654N
IntActiP22415, 33 interactors
MINTiP22415
STRINGi9606.ENSP00000356999

Structurei

Secondary structure

1310
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi205 – 225Combined sources21
Beta strandi233 – 236Combined sources4
Turni240 – 247Combined sources8
Helixi248 – 255Combined sources8
Turni256 – 258Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AN4X-ray2.90A/B197-260[»]
ProteinModelPortaliP22415
SMRiP22415
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22415

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini199 – 254bHLHPROSITE-ProRule annotationAdd BLAST56

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni271 – 292Leucine-zipperAdd BLAST22

Phylogenomic databases

eggNOGiENOG410INSE Eukaryota
ENOG4110B3H LUCA
GeneTreeiENSGT00390000004498
HOGENOMiHOG000294174
HOVERGENiHBG004346
InParanoidiP22415
KOiK09106
OMAiNQRTIAP
OrthoDBiEOG091G0RH5
PhylomeDBiP22415
TreeFamiTF323338

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P22415-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKGQQKTAET EEGTVQIQEG AVATGEDPTS VAIASIQSAA TFPDPNVKYV
60 70 80 90 100
FRTENGGQVM YRVIQVSEGQ LDGQTEGTGA ISGYPATQSM TQAVIQGAFT
110 120 130 140 150
SDDAVDTEGT AAETHYTYFP STAVGDGAGG TTSGSTAAVV TTQGSEALLG
160 170 180 190 200
QATPPGTGQF FVMMSPQEVL QGGSQRSIAP RTHPYSPKSE APRTTRDEKR
210 220 230 240 250
RAQHNEVERR RRDKINNWIV QLSKIIPDCS MESTKSGQSK GGILSKACDY
260 270 280 290 300
IQELRQSNHR LSEELQGLDQ LQLDNDVLRQ QVEDLKNKNL LLRAQLRHHG
310
LEVVIKNDSN
Length:310
Mass (Da):33,538
Last modified:August 1, 1991 - v1
Checksum:iBFDA91519B4B80AE
GO
Isoform 2 (identifier: P22415-2) [UniParc]FASTAAdd to basket
Also known as: usf1-bd

The sequence of this isoform differs from the canonical sequence as follows:
     1-59: Missing.

Show »
Length:251
Mass (Da):27,372
Checksum:iAA50D545E179EFCB
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0477401 – 59Missing in isoform 2. 1 PublicationAdd BLAST59

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55666 mRNA Translation: CAA39201.1
AB017568 Genomic DNA Translation: BAA76541.1
AB098540 mRNA Translation: BAC78384.1
AK314876 mRNA Translation: BAG37391.1
AL832119 mRNA No translation available.
AY593992 Genomic DNA Translation: AAS89301.1
AL591806 Genomic DNA No translation available.
CH471121 Genomic DNA Translation: EAW52675.1
BC035505 mRNA Translation: AAH35505.1
CCDSiCCDS1214.1 [P22415-1]
PIRiS13525
RefSeqiNP_001263302.1, NM_001276373.1 [P22415-1]
NP_009053.1, NM_007122.4 [P22415-1]
NP_996888.1, NM_207005.2 [P22415-2]
UniGeneiHs.414880

Genome annotation databases

EnsembliENST00000368020; ENSP00000356999; ENSG00000158773 [P22415-1]
ENST00000368021; ENSP00000357000; ENSG00000158773 [P22415-1]
GeneIDi7391
KEGGihsa:7391
UCSCiuc001fxi.5 human [P22415-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUSF1_HUMAN
AccessioniPrimary (citable) accession number: P22415
Secondary accession number(s): B2RBZ4, Q5SY46, Q7Z5Y1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: May 23, 2018
This is version 177 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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