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P22415

- USF1_HUMAN

UniProt

P22415 - USF1_HUMAN

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Protein
Upstream stimulatory factor 1
Gene
USF1, BHLHB11, USF
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

GO - Molecular functioni

  1. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: BHF-UCL
  2. bHLH transcription factor binding Source: BHF-UCL
  3. double-stranded DNA binding Source: Ensembl
  4. enzyme binding Source: BHF-UCL
  5. histone deacetylase binding Source: BHF-UCL
  6. protein binding Source: IntAct
  7. protein heterodimerization activity Source: BHF-UCL
  8. protein homodimerization activity Source: BHF-UCL
  9. protein kinase binding Source: BHF-UCL
  10. sequence-specific DNA binding Source: BHF-UCL

GO - Biological processi

  1. carbon catabolite regulation of transcription Source: BHF-UCL
  2. cellular response to insulin stimulus Source: BHF-UCL
  3. glucose homeostasis Source: BHF-UCL
  4. glucose metabolic process Source: Ensembl
  5. late viral transcription Source: BHF-UCL
  6. lipid homeostasis Source: BHF-UCL
  7. negative regulation of fibrinolysis Source: BHF-UCL
  8. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  9. positive regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
  10. regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  11. regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
  12. response to UV Source: BHF-UCL
  13. response to hypoxia Source: BHF-UCL
  14. transcription from RNA polymerase II promoter Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

SignaLinkiP22415.

Names & Taxonomyi

Protein namesi
Recommended name:
Upstream stimulatory factor 1
Alternative name(s):
Class B basic helix-loop-helix protein 11
Short name:
bHLHb11
Major late transcription factor 1
Gene namesi
Name:USF1
Synonyms:BHLHB11, USF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:12593. USF1.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: BHF-UCL
  2. transcription factor complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Hyperlipidemia combined 1 (HYPLIP1) [MIM:602491]: A disorder characterized by a variable pattern of elevated levels of serum total cholesterol, triglycerides or both. It is observed in a percentage of individuals with premature coronary heart disease.
Note: Disease susceptibility is associated with variations affecting the gene represented in this entry.1 Publication

Organism-specific databases

MIMi144250. phenotype.
602491. phenotype.
PharmGKBiPA37223.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310Upstream stimulatory factor 1
PRO_0000127496Add
BLAST

Proteomic databases

MaxQBiP22415.
PaxDbiP22415.
PRIDEiP22415.

PTM databases

PhosphoSiteiP22415.

Expressioni

Gene expression databases

ArrayExpressiP22415.
BgeeiP22415.
CleanExiHS_USF1.
GenevestigatoriP22415.

Organism-specific databases

HPAiCAB001480.
HPA036233.
HPA036535.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2). Interacts with varicella-zoster virus IE62 protein.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
KAT2BQ928315EBI-1054489,EBI-477430

Protein-protein interaction databases

BioGridi113237. 41 interactions.
DIPiDIP-654N.
IntActiP22415. 13 interactions.
STRINGi9606.ENSP00000356999.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi205 – 22521
Beta strandi233 – 2364
Turni240 – 2478
Helixi248 – 2558
Turni256 – 2583

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AN4X-ray2.90A/B197-260[»]
ProteinModelPortaliP22415.
SMRiP22415. Positions 197-260.

Miscellaneous databases

EvolutionaryTraceiP22415.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini199 – 25456bHLH
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni271 – 29222Leucine-zipper
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG272240.
HOGENOMiHOG000294174.
HOVERGENiHBG004346.
InParanoidiP22415.
KOiK09106.
OMAiHEVELTN.
OrthoDBiEOG7GXPBW.
PhylomeDBiP22415.
TreeFamiTF323338.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P22415-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MKGQQKTAET EEGTVQIQEG AVATGEDPTS VAIASIQSAA TFPDPNVKYV    50
FRTENGGQVM YRVIQVSEGQ LDGQTEGTGA ISGYPATQSM TQAVIQGAFT 100
SDDAVDTEGT AAETHYTYFP STAVGDGAGG TTSGSTAAVV TTQGSEALLG 150
QATPPGTGQF FVMMSPQEVL QGGSQRSIAP RTHPYSPKSE APRTTRDEKR 200
RAQHNEVERR RRDKINNWIV QLSKIIPDCS MESTKSGQSK GGILSKACDY 250
IQELRQSNHR LSEELQGLDQ LQLDNDVLRQ QVEDLKNKNL LLRAQLRHHG 300
LEVVIKNDSN 310
Length:310
Mass (Da):33,538
Last modified:August 1, 1991 - v1
Checksum:iBFDA91519B4B80AE
GO
Isoform 2 (identifier: P22415-2) [UniParc]FASTAAdd to Basket

Also known as: usf1-bd

The sequence of this isoform differs from the canonical sequence as follows:
     1-59: Missing.

Show »
Length:251
Mass (Da):27,372
Checksum:iAA50D545E179EFCB
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5959Missing in isoform 2.
VSP_047740Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55666 mRNA. Translation: CAA39201.1.
AB017568 Genomic DNA. Translation: BAA76541.1.
AB098540 mRNA. Translation: BAC78384.1.
AK314876 mRNA. Translation: BAG37391.1.
AL832119 mRNA. No translation available.
AY593992 Genomic DNA. Translation: AAS89301.1.
AL591806 Genomic DNA. Translation: CAI15372.1.
CH471121 Genomic DNA. Translation: EAW52675.1.
BC035505 mRNA. Translation: AAH35505.1.
CCDSiCCDS1214.1. [P22415-1]
PIRiS13525.
RefSeqiNP_001263302.1. NM_001276373.1. [P22415-1]
NP_009053.1. NM_007122.4. [P22415-1]
NP_996888.1. NM_207005.2. [P22415-2]
UniGeneiHs.414880.

Genome annotation databases

EnsembliENST00000368020; ENSP00000356999; ENSG00000158773. [P22415-1]
ENST00000368021; ENSP00000357000; ENSG00000158773. [P22415-1]
ENST00000435396; ENSP00000390109; ENSG00000158773. [P22415-2]
GeneIDi7391.
KEGGihsa:7391.
UCSCiuc001fxi.4. human. [P22415-1]

Polymorphism databases

DMDMi137170.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

SeattleSNPs
Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55666 mRNA. Translation: CAA39201.1 .
AB017568 Genomic DNA. Translation: BAA76541.1 .
AB098540 mRNA. Translation: BAC78384.1 .
AK314876 mRNA. Translation: BAG37391.1 .
AL832119 mRNA. No translation available.
AY593992 Genomic DNA. Translation: AAS89301.1 .
AL591806 Genomic DNA. Translation: CAI15372.1 .
CH471121 Genomic DNA. Translation: EAW52675.1 .
BC035505 mRNA. Translation: AAH35505.1 .
CCDSi CCDS1214.1. [P22415-1 ]
PIRi S13525.
RefSeqi NP_001263302.1. NM_001276373.1. [P22415-1 ]
NP_009053.1. NM_007122.4. [P22415-1 ]
NP_996888.1. NM_207005.2. [P22415-2 ]
UniGenei Hs.414880.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AN4 X-ray 2.90 A/B 197-260 [» ]
ProteinModelPortali P22415.
SMRi P22415. Positions 197-260.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 113237. 41 interactions.
DIPi DIP-654N.
IntActi P22415. 13 interactions.
STRINGi 9606.ENSP00000356999.

PTM databases

PhosphoSitei P22415.

Polymorphism databases

DMDMi 137170.

Proteomic databases

MaxQBi P22415.
PaxDbi P22415.
PRIDEi P22415.

Protocols and materials databases

DNASUi 7391.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000368020 ; ENSP00000356999 ; ENSG00000158773 . [P22415-1 ]
ENST00000368021 ; ENSP00000357000 ; ENSG00000158773 . [P22415-1 ]
ENST00000435396 ; ENSP00000390109 ; ENSG00000158773 . [P22415-2 ]
GeneIDi 7391.
KEGGi hsa:7391.
UCSCi uc001fxi.4. human. [P22415-1 ]

Organism-specific databases

CTDi 7391.
GeneCardsi GC01M161009.
HGNCi HGNC:12593. USF1.
HPAi CAB001480.
HPA036233.
HPA036535.
MIMi 144250. phenotype.
191523. gene.
602491. phenotype.
neXtProti NX_P22415.
PharmGKBi PA37223.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG272240.
HOGENOMi HOG000294174.
HOVERGENi HBG004346.
InParanoidi P22415.
KOi K09106.
OMAi HEVELTN.
OrthoDBi EOG7GXPBW.
PhylomeDBi P22415.
TreeFami TF323338.

Enzyme and pathway databases

SignaLinki P22415.

Miscellaneous databases

ChiTaRSi USF1. human.
EvolutionaryTracei P22415.
GeneWikii USF1.
GenomeRNAii 7391.
NextBioi 28938.
PROi P22415.
SOURCEi Search...

Gene expression databases

ArrayExpressi P22415.
Bgeei P22415.
CleanExi HS_USF1.
Genevestigatori P22415.

Family and domain databases

Gene3Di 4.10.280.10. 1 hit.
InterProi IPR011598. bHLH_dom.
[Graphical view ]
Pfami PF00010. HLH. 1 hit.
[Graphical view ]
SMARTi SM00353. HLH. 1 hit.
[Graphical view ]
SUPFAMi SSF47459. SSF47459. 1 hit.
PROSITEi PS50888. BHLH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The adenovirus major late transcription factor USF is a member of the helix-loop-helix group of regulatory proteins and binds to DNA as a dimer."
    Gregor P.D., Sawadogo M., Roeder R.G.
    Genes Dev. 4:1730-1740(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 1-25.
  2. "Human USF1 genomic sequence."
    Fukamizu A.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Cloning and characterization of a novel splicing isoform of USF1."
    Saito T., Oishi T., Yanai K., Shimamoto Y., Fukamizu A.
    Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Fetal kidney.
  6. SeattleSNPs variation discovery resource
    Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  7. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Kidney.
  10. "Transcription factor USF, expressed during the entire phase of Varicella-zoster virus infection, interacts physically with the major viral transactivator IE62 and plays a significant role in virus replication."
    Rahaus M., Desloges N., Yang M., Ruyechan W.T., Wolff M.H.
    J. Gen. Virol. 84:2957-2967(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VARICELLA-ZOSTER VIRUS IE62 PROTEIN.
  11. "Structure and function of the b/HLH/Z domain of USF."
    Ferre-D'Amare A.R., Pognonec P., Roeder R.G., Burley S.K.
    EMBO J. 13:180-189(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 197-260.
  12. Cited for: INVOLVEMENT IN HYPLIP1.

Entry informationi

Entry nameiUSF1_HUMAN
AccessioniPrimary (citable) accession number: P22415
Secondary accession number(s): B2RBZ4, Q5SY46, Q7Z5Y1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: July 9, 2014
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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