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Protein

Nucleoside diphosphate kinase B

Gene

NME2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity.3 Publications

Catalytic activityi

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12ATP1
Binding sitei60ATP1
Binding sitei88ATP1
Binding sitei94ATP1
Binding sitei105ATP1
Binding sitei115ATP1
Active sitei118Pros-phosphohistidine intermediate1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Transferase

Keywords - Biological processi

Nucleotide metabolism, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS04463-MONOMER.
ZFISH:HS04463-MONOMER.
ReactomeiR-HSA-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
R-HSA-6798695. Neutrophil degranulation.
SignaLinkiP22392.
SIGNORiP22392.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside diphosphate kinase B (EC:2.7.4.6)
Short name:
NDK B
Short name:
NDP kinase B
Alternative name(s):
C-myc purine-binding transcription factor PUF
Histidine protein kinase NDKB (EC:2.7.13.3)
nm23-H2
Gene namesi
Name:NME2
Synonyms:NM23B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:7850. NME2.

Subcellular locationi

GO - Cellular componenti

  • cell periphery Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • intermediate filament Source: Ensembl
  • lamellipodium Source: HGNC
  • mitochondrial membrane Source: Ensembl
  • myelin sheath Source: Ensembl
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: Ensembl
  • ruffle Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4831.
OpenTargetsiENSG00000011052.
ENSG00000243678.
PharmGKBiPA162398077.

Chemistry databases

ChEMBLiCHEMBL2160.
DrugBankiDB00718. Adefovir Dipivoxil.
DB00709. Lamivudine.
DB00300. Tenofovir.

Polymorphism and mutation databases

BioMutaiNME2.
DMDMi127983.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001371171 – 152Nucleoside diphosphate kinase BAdd BLAST152

Proteomic databases

EPDiP22392.
PaxDbiP22392.
PeptideAtlasiP22392.
PRIDEiP22392.
TopDownProteomicsiP22392-1. [P22392-1]
P22392-2. [P22392-2]

2D gel databases

DOSAC-COBS-2DPAGEP22392.
OGPiP22392.
UCD-2DPAGEP22392.

PTM databases

iPTMnetiP22392.
PhosphoSitePlusiP22392.
SwissPalmiP22392.

Expressioni

Tissue specificityi

Isoform 1 and isoform 3 are ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000243678.
ExpressionAtlasiP22392. baseline and differential.
GenevisibleiP22392. HS.

Organism-specific databases

HPAiCAB002169.
HPA041113.

Interactioni

Subunit structurei

Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with CAPN8 (By similarity). Interacts with AKAP13. Interacts ITGB1BP1 (via C-terminal domain region).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ITGB1BP1O14713-17EBI-713693,EBI-2127367
NME1P155312EBI-713693,EBI-741141

Protein-protein interaction databases

BioGridi110895. 83 interactors.
576341. 45 interactors.
DIPiDIP-50179N.
IntActiP22392. 20 interactors.
MINTiMINT-1429922.
STRINGi9606.ENSP00000376886.

Structurei

Secondary structure

1152
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 11Combined sources6
Helixi13 – 17Combined sources5
Helixi21 – 31Combined sources11
Beta strandi34 – 41Combined sources8
Helixi45 – 51Combined sources7
Helixi53 – 55Combined sources3
Helixi61 – 68Combined sources8
Beta strandi73 – 80Combined sources8
Helixi83 – 91Combined sources9
Helixi96 – 98Combined sources3
Helixi104 – 108Combined sources5
Helixi112 – 114Combined sources3
Beta strandi117 – 119Combined sources3
Helixi123 – 133Combined sources11
Helixi136 – 138Combined sources3
Helixi147 – 150Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NSKX-ray2.80L/N/O/R/T/U1-152[»]
1NUEX-ray2.00A/B/C/D/E/F2-152[»]
3BBBX-ray1.30A/B/C/D/E/F2-152[»]
3BBCX-ray1.70A/B/C/D/E/F2-152[»]
3BBFX-ray1.70A/B/C/D/E/F2-152[»]
ProteinModelPortaliP22392.
SMRiP22392.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22392.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 66Interaction with AKAP131 PublicationAdd BLAST66

Sequence similaritiesi

Belongs to the NDK family.Curated

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
COG0105. LUCA.
GeneTreeiENSGT00760000119146.
HOGENOMiHOG000224564.
HOVERGENiHBG000423.
InParanoidiP22392.
KOiK00940.
PhylomeDBiP22392.
TreeFamiTF106373.

Family and domain databases

HAMAPiMF_00451. NDP_kinase. 1 hit.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
PfamiPF00334. NDK. 1 hit.
[Graphical view]
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]
PROSITEiPS00469. NDP_KINASES. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P22392-1) [UniParc]FASTAAdd to basket
Also known as: NM23-H2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ
60 70 80 90 100
HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK
110 120 130 140 150
PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV

YE
Length:152
Mass (Da):17,298
Last modified:August 1, 1991 - v1
Checksum:i1A5C3F84D7AD272C
GO
Isoform 3 (identifier: P22392-2) [UniParc]FASTAAdd to basket
Also known as: NM23-LV

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MANCERTFIA...DFCIQVGRTM

Note: Based on a naturally occurring readthrough transcript which produces an NME1-NME2 fusion protein.By similarity
Show »
Length:267
Mass (Da):30,137
Checksum:i3AB02272AF24AAC3
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0367081M → MANCERTFIAIKPDGVQRGL VGEIIKRFEQKGFRLVGLKF MQASEDLLKEHYVDLKDRPF FAGLVKYMHSGPVVAMVWEG LNVVKTGRVMLGETNPADSK PGTIRGDFCIQVGRTM in isoform 3. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58965 mRNA. Translation: CAB37870.1.
M36981 mRNA. Translation: AAA36369.1.
L16785 mRNA. Translation: AAA60228.1.
DQ109675 mRNA. Translation: AAZ82097.1.
AC005839 Genomic DNA. No translation available.
BC002476 mRNA. Translation: AAH02476.1.
BC133029 mRNA. Translation: AAI33030.1.
BC133031 mRNA. Translation: AAI33032.1.
U29200 Genomic DNA. Translation: AAA86745.1.
CCDSiCCDS11580.1. [P22392-1]
PIRiA49798.
RefSeqiNP_001018146.1. NM_001018136.2. [P22392-2]
NP_001018147.1. NM_001018137.2. [P22392-1]
NP_001018148.1. NM_001018138.1. [P22392-1]
NP_001018149.1. NM_001018139.2. [P22392-1]
NP_002503.1. NM_002512.3. [P22392-1]
UniGeneiHs.463456.

Genome annotation databases

EnsembliENST00000393190; ENSP00000376886; ENSG00000243678. [P22392-1]
ENST00000503064; ENSP00000426901; ENSG00000243678. [P22392-1]
ENST00000512737; ENSP00000421064; ENSG00000243678. [P22392-1]
ENST00000513177; ENSP00000425581; ENSG00000243678. [P22392-1]
ENST00000514264; ENSP00000426976; ENSG00000243678. [P22392-1]
GeneIDi4831.
654364.
KEGGihsa:4831.
hsa:654364.
UCSCiuc002itj.4. human. [P22392-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58965 mRNA. Translation: CAB37870.1.
M36981 mRNA. Translation: AAA36369.1.
L16785 mRNA. Translation: AAA60228.1.
DQ109675 mRNA. Translation: AAZ82097.1.
AC005839 Genomic DNA. No translation available.
BC002476 mRNA. Translation: AAH02476.1.
BC133029 mRNA. Translation: AAI33030.1.
BC133031 mRNA. Translation: AAI33032.1.
U29200 Genomic DNA. Translation: AAA86745.1.
CCDSiCCDS11580.1. [P22392-1]
PIRiA49798.
RefSeqiNP_001018146.1. NM_001018136.2. [P22392-2]
NP_001018147.1. NM_001018137.2. [P22392-1]
NP_001018148.1. NM_001018138.1. [P22392-1]
NP_001018149.1. NM_001018139.2. [P22392-1]
NP_002503.1. NM_002512.3. [P22392-1]
UniGeneiHs.463456.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NSKX-ray2.80L/N/O/R/T/U1-152[»]
1NUEX-ray2.00A/B/C/D/E/F2-152[»]
3BBBX-ray1.30A/B/C/D/E/F2-152[»]
3BBCX-ray1.70A/B/C/D/E/F2-152[»]
3BBFX-ray1.70A/B/C/D/E/F2-152[»]
ProteinModelPortaliP22392.
SMRiP22392.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110895. 83 interactors.
576341. 45 interactors.
DIPiDIP-50179N.
IntActiP22392. 20 interactors.
MINTiMINT-1429922.
STRINGi9606.ENSP00000376886.

Chemistry databases

ChEMBLiCHEMBL2160.
DrugBankiDB00718. Adefovir Dipivoxil.
DB00709. Lamivudine.
DB00300. Tenofovir.

PTM databases

iPTMnetiP22392.
PhosphoSitePlusiP22392.
SwissPalmiP22392.

Polymorphism and mutation databases

BioMutaiNME2.
DMDMi127983.

2D gel databases

DOSAC-COBS-2DPAGEP22392.
OGPiP22392.
UCD-2DPAGEP22392.

Proteomic databases

EPDiP22392.
PaxDbiP22392.
PeptideAtlasiP22392.
PRIDEiP22392.
TopDownProteomicsiP22392-1. [P22392-1]
P22392-2. [P22392-2]

Protocols and materials databases

DNASUi4831.
654364.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000393190; ENSP00000376886; ENSG00000243678. [P22392-1]
ENST00000503064; ENSP00000426901; ENSG00000243678. [P22392-1]
ENST00000512737; ENSP00000421064; ENSG00000243678. [P22392-1]
ENST00000513177; ENSP00000425581; ENSG00000243678. [P22392-1]
ENST00000514264; ENSP00000426976; ENSG00000243678. [P22392-1]
GeneIDi4831.
654364.
KEGGihsa:4831.
hsa:654364.
UCSCiuc002itj.4. human. [P22392-1]

Organism-specific databases

CTDi4831.
654364.
DisGeNETi4831.
GeneCardsiNME2.
HGNCiHGNC:7850. NME2.
HPAiCAB002169.
HPA041113.
MIMi156491. gene.
neXtProtiNX_P22392.
OpenTargetsiENSG00000011052.
ENSG00000243678.
PharmGKBiPA162398077.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
COG0105. LUCA.
GeneTreeiENSGT00760000119146.
HOGENOMiHOG000224564.
HOVERGENiHBG000423.
InParanoidiP22392.
KOiK00940.
PhylomeDBiP22392.
TreeFamiTF106373.

Enzyme and pathway databases

BioCyciMetaCyc:HS04463-MONOMER.
ZFISH:HS04463-MONOMER.
ReactomeiR-HSA-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
R-HSA-6798695. Neutrophil degranulation.
SignaLinkiP22392.
SIGNORiP22392.

Miscellaneous databases

ChiTaRSiNME2. human.
EvolutionaryTraceiP22392.
GeneWikiiNME1-NME2.
NME2.
PROiP22392.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000243678.
ExpressionAtlasiP22392. baseline and differential.
GenevisibleiP22392. HS.

Family and domain databases

HAMAPiMF_00451. NDP_kinase. 1 hit.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
PfamiPF00334. NDK. 1 hit.
[Graphical view]
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]
PROSITEiPS00469. NDP_KINASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDKB_HUMAN
AccessioniPrimary (citable) accession number: P22392
Secondary accession number(s): A8MWA3, Q1WM23, Q6LCT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: November 30, 2016
This is version 201 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.