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P22391

- BLO1_KLEOX

UniProt

P22391 - BLO1_KLEOX

Protein

Beta-lactamase OXY-1

Gene

bla

Organism
Klebsiella oxytoca
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Aug 1991)
      Previous versions | rss
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    Functioni

    Hydrolyzes broad-spectrum beta-lactam antibiotics. Active against cephalosporins.

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei73 – 731Acyl-ester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase OXY-1 (EC:3.5.2.6)
    Alternative name(s):
    Penicillinase
    Gene namesi
    Name:bla
    OrganismiKlebsiella oxytoca
    Taxonomic identifieri571 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Add
    BLAST
    Chaini25 – 291267Beta-lactamase OXY-1PRO_0000016997Add
    BLAST

    Structurei

    Secondary structure

    1
    291
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi32 – 4413
    Beta strandi46 – 549
    Turni55 – 573
    Beta strandi60 – 645
    Helixi72 – 754
    Helixi76 – 8914
    Beta strandi91 – 933
    Helixi102 – 1043
    Helixi112 – 1143
    Turni116 – 1183
    Helixi122 – 13110
    Helixi135 – 14511
    Helixi148 – 15710
    Helixi171 – 1733
    Helixi186 – 19712
    Beta strandi199 – 2024
    Helixi204 – 21512
    Turni221 – 2233
    Helixi224 – 2274
    Beta strandi232 – 24110
    Turni242 – 2443
    Beta strandi245 – 2539
    Beta strandi255 – 2573
    Beta strandi260 – 2678
    Helixi277 – 28812

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3BYDX-ray1.93A25-291[»]
    ProteinModelPortaliP22391.
    SMRiP22391. Positions 31-291.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP22391.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni237 – 2393Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PfamiPF00144. Beta-lactamase. 1 hit.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P22391-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKSSWRKTA LMAAAAVPLL LASGSLWASA DAIQQKLADL EKRSGGRLGV    50
    ALINTADDSQ TLYRGDERFA MCSTGKVMAA AAVLKQSESN PEVVNKRLEI 100
    KKSDLVVWSP ITEKHLQSGM TLAELSAAAL QYSDNTAMNK MISYLGGPEK 150
    VTAFAQSIGD VTFRLDRTEP ALNSAIPGDK RDTTTPLAMA ESLRKLTLGN 200
    ALGEQQRAQL VTWLKGNTTG GQSIRAGLPA SWAVGDKTGA GDYGTTNDIA 250
    VIWPENHAPL VLVTYFTQPQ QDAKSRKEVL AAAAKIVTEG L 291
    Length:291
    Mass (Da):31,044
    Last modified:August 1, 1991 - v1
    Checksum:i9908F5AA97EDDC8A
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti262 – 2621L → P in strain: KH66.
    Natural varianti278 – 2781E → K in strain: KH66.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27459 Genomic DNA. Translation: AAA25084.1.
    Z30177 Genomic DNA. Translation: CAA82916.1.
    Z30178 Genomic DNA. Translation: CAA82917.1.
    Y17715 Genomic DNA. Translation: CAB42615.1.
    PIRiS42075.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27459 Genomic DNA. Translation: AAA25084.1 .
    Z30177 Genomic DNA. Translation: CAA82916.1 .
    Z30178 Genomic DNA. Translation: CAA82917.1 .
    Y17715 Genomic DNA. Translation: CAB42615.1 .
    PIRi S42075.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3BYD X-ray 1.93 A 25-291 [» ]
    ProteinModelPortali P22391.
    SMRi P22391. Positions 31-291.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P22391.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view ]
    Pfami PF00144. Beta-lactamase. 1 hit.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Chromosomal beta-lactamase of Klebsiella oxytoca, a new class A enzyme that hydrolyzes broad-spectrum beta-lactam antibiotics."
      Arakawa Y., Ohta M., Kido N., Mori M., Ito H., Komatsu T., Fujii Y., Kato N.
      Antimicrob. Agents Chemother. 33:63-70(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: E23004.
    2. "Klebsiella oxytoca: resistance to aztreonam by overproduction of the chromosomally encoded beta-lactamase."
      Fournier B., Arlet G., Lagrange P.H., Philippon A.
      FEMS Microbiol. Lett. 116:31-36(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SL781 and SL7811.
    3. "Genetic characterization of resistance to extended-spectrum beta-lactams in Klebsiella oxytoca isolates recovered from patients with septicemia at hospitals in the Stockholm area."
      Wu S.W., Dornbusch K., Kronvall G.
      Antimicrob. Agents Chemother. 43:1294-1297(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: KH66.

    Entry informationi

    Entry nameiBLO1_KLEOX
    AccessioniPrimary (citable) accession number: P22391
    Secondary accession number(s): Q9S6S0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In strain KH66 OXY-1 is known as OXY-1a.

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3