SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P22391

- BLO1_KLEOX

UniProt

P22391 - BLO1_KLEOX

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Beta-lactamase OXY-1
Gene
bla
Organism
Klebsiella oxytoca
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes broad-spectrum beta-lactam antibiotics. Active against cephalosporins.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731Acyl-ester intermediate By similarity

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase OXY-1 (EC:3.5.2.6)
Alternative name(s):
Penicillinase
Gene namesi
Name:bla
OrganismiKlebsiella oxytoca
Taxonomic identifieri571 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424
Add
BLAST
Chaini25 – 291267Beta-lactamase OXY-1
PRO_0000016997Add
BLAST

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi32 – 4413
Beta strandi46 – 549
Turni55 – 573
Beta strandi60 – 645
Helixi72 – 754
Helixi76 – 8914
Beta strandi91 – 933
Helixi102 – 1043
Helixi112 – 1143
Turni116 – 1183
Helixi122 – 13110
Helixi135 – 14511
Helixi148 – 15710
Helixi171 – 1733
Helixi186 – 19712
Beta strandi199 – 2024
Helixi204 – 21512
Turni221 – 2233
Helixi224 – 2274
Beta strandi232 – 24110
Turni242 – 2443
Beta strandi245 – 2539
Beta strandi255 – 2573
Beta strandi260 – 2678
Helixi277 – 28812

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3BYDX-ray1.93A25-291[»]
ProteinModelPortaliP22391.
SMRiP22391. Positions 31-291.

Miscellaneous databases

EvolutionaryTraceiP22391.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni237 – 2393Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22391-1 [UniParc]FASTAAdd to Basket

« Hide

MLKSSWRKTA LMAAAAVPLL LASGSLWASA DAIQQKLADL EKRSGGRLGV    50
ALINTADDSQ TLYRGDERFA MCSTGKVMAA AAVLKQSESN PEVVNKRLEI 100
KKSDLVVWSP ITEKHLQSGM TLAELSAAAL QYSDNTAMNK MISYLGGPEK 150
VTAFAQSIGD VTFRLDRTEP ALNSAIPGDK RDTTTPLAMA ESLRKLTLGN 200
ALGEQQRAQL VTWLKGNTTG GQSIRAGLPA SWAVGDKTGA GDYGTTNDIA 250
VIWPENHAPL VLVTYFTQPQ QDAKSRKEVL AAAAKIVTEG L 291
Length:291
Mass (Da):31,044
Last modified:August 1, 1991 - v1
Checksum:i9908F5AA97EDDC8A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti262 – 2621L → P in strain: KH66.
Natural varianti278 – 2781E → K in strain: KH66.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27459 Genomic DNA. Translation: AAA25084.1.
Z30177 Genomic DNA. Translation: CAA82916.1.
Z30178 Genomic DNA. Translation: CAA82917.1.
Y17715 Genomic DNA. Translation: CAB42615.1.
PIRiS42075.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27459 Genomic DNA. Translation: AAA25084.1 .
Z30177 Genomic DNA. Translation: CAA82916.1 .
Z30178 Genomic DNA. Translation: CAA82917.1 .
Y17715 Genomic DNA. Translation: CAB42615.1 .
PIRi S42075.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3BYD X-ray 1.93 A 25-291 [» ]
ProteinModelPortali P22391.
SMRi P22391. Positions 31-291.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P22391.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view ]
Pfami PF00144. Beta-lactamase. 1 hit.
[Graphical view ]
PRINTSi PR00118. BLACTAMASEA.
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Chromosomal beta-lactamase of Klebsiella oxytoca, a new class A enzyme that hydrolyzes broad-spectrum beta-lactam antibiotics."
    Arakawa Y., Ohta M., Kido N., Mori M., Ito H., Komatsu T., Fujii Y., Kato N.
    Antimicrob. Agents Chemother. 33:63-70(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: E23004.
  2. "Klebsiella oxytoca: resistance to aztreonam by overproduction of the chromosomally encoded beta-lactamase."
    Fournier B., Arlet G., Lagrange P.H., Philippon A.
    FEMS Microbiol. Lett. 116:31-36(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SL781 and SL7811.
  3. "Genetic characterization of resistance to extended-spectrum beta-lactams in Klebsiella oxytoca isolates recovered from patients with septicemia at hospitals in the Stockholm area."
    Wu S.W., Dornbusch K., Kronvall G.
    Antimicrob. Agents Chemother. 43:1294-1297(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: KH66.

Entry informationi

Entry nameiBLO1_KLEOX
AccessioniPrimary (citable) accession number: P22391
Secondary accession number(s): Q9S6S0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: September 3, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In strain KH66 OXY-1 is known as OXY-1a.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi