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Protein

Beta-lactamase OXY-1

Gene

bla

Organism
Klebsiella oxytoca
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes broad-spectrum beta-lactam antibiotics. Active against cephalosporins.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei73Acyl-ester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase OXY-1 (EC:3.5.2.6)
Alternative name(s):
Penicillinase
Gene namesi
Name:bla
OrganismiKlebsiella oxytoca
Taxonomic identifieri571 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Add BLAST24
ChainiPRO_000001699725 – 291Beta-lactamase OXY-1Add BLAST267

Interactioni

Protein-protein interaction databases

STRINGi1006551.KOX_13290.

Structurei

Secondary structure

1291
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi32 – 44Combined sources13
Beta strandi46 – 54Combined sources9
Turni55 – 57Combined sources3
Beta strandi60 – 64Combined sources5
Helixi72 – 75Combined sources4
Helixi76 – 89Combined sources14
Beta strandi91 – 93Combined sources3
Helixi102 – 104Combined sources3
Helixi112 – 114Combined sources3
Turni116 – 118Combined sources3
Helixi122 – 131Combined sources10
Helixi135 – 145Combined sources11
Helixi148 – 157Combined sources10
Helixi171 – 173Combined sources3
Helixi186 – 197Combined sources12
Beta strandi199 – 202Combined sources4
Helixi204 – 215Combined sources12
Turni221 – 223Combined sources3
Helixi224 – 227Combined sources4
Beta strandi232 – 241Combined sources10
Turni242 – 244Combined sources3
Beta strandi245 – 253Combined sources9
Beta strandi255 – 257Combined sources3
Beta strandi260 – 267Combined sources8
Helixi277 – 288Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BYDX-ray1.93A25-291[»]
ProteinModelPortaliP22391.
SMRiP22391.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22391.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni237 – 239Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108J4B. Bacteria.
COG2367. LUCA.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22391-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKSSWRKTA LMAAAAVPLL LASGSLWASA DAIQQKLADL EKRSGGRLGV
60 70 80 90 100
ALINTADDSQ TLYRGDERFA MCSTGKVMAA AAVLKQSESN PEVVNKRLEI
110 120 130 140 150
KKSDLVVWSP ITEKHLQSGM TLAELSAAAL QYSDNTAMNK MISYLGGPEK
160 170 180 190 200
VTAFAQSIGD VTFRLDRTEP ALNSAIPGDK RDTTTPLAMA ESLRKLTLGN
210 220 230 240 250
ALGEQQRAQL VTWLKGNTTG GQSIRAGLPA SWAVGDKTGA GDYGTTNDIA
260 270 280 290
VIWPENHAPL VLVTYFTQPQ QDAKSRKEVL AAAAKIVTEG L
Length:291
Mass (Da):31,044
Last modified:August 1, 1991 - v1
Checksum:i9908F5AA97EDDC8A
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti262L → P in strain: KH66. 1
Natural varianti278E → K in strain: KH66. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27459 Genomic DNA. Translation: AAA25084.1.
Z30177 Genomic DNA. Translation: CAA82916.1.
Z30178 Genomic DNA. Translation: CAA82917.1.
Y17715 Genomic DNA. Translation: CAB42615.1.
PIRiS42075.
RefSeqiWP_014228247.1. NZ_FKZD01000003.1.
WP_063864546.1. NG_049845.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27459 Genomic DNA. Translation: AAA25084.1.
Z30177 Genomic DNA. Translation: CAA82916.1.
Z30178 Genomic DNA. Translation: CAA82917.1.
Y17715 Genomic DNA. Translation: CAB42615.1.
PIRiS42075.
RefSeqiWP_014228247.1. NZ_FKZD01000003.1.
WP_063864546.1. NG_049845.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BYDX-ray1.93A25-291[»]
ProteinModelPortaliP22391.
SMRiP22391.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1006551.KOX_13290.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4108J4B. Bacteria.
COG2367. LUCA.

Miscellaneous databases

EvolutionaryTraceiP22391.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBLO1_KLEOX
AccessioniPrimary (citable) accession number: P22391
Secondary accession number(s): Q9S6S0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: November 2, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In strain KH66 OXY-1 is known as OXY-1a.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.