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P22391

- BLO1_KLEOX

UniProt

P22391 - BLO1_KLEOX

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Protein

Beta-lactamase OXY-1

Gene

bla

Organism
Klebsiella oxytoca
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes broad-spectrum beta-lactam antibiotics. Active against cephalosporins.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731Acyl-ester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase OXY-1 (EC:3.5.2.6)
Alternative name(s):
Penicillinase
Gene namesi
Name:bla
OrganismiKlebsiella oxytoca
Taxonomic identifieri571 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Add
BLAST
Chaini25 – 291267Beta-lactamase OXY-1PRO_0000016997Add
BLAST

Structurei

Secondary structure

1
291
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi32 – 4413Combined sources
Beta strandi46 – 549Combined sources
Turni55 – 573Combined sources
Beta strandi60 – 645Combined sources
Helixi72 – 754Combined sources
Helixi76 – 8914Combined sources
Beta strandi91 – 933Combined sources
Helixi102 – 1043Combined sources
Helixi112 – 1143Combined sources
Turni116 – 1183Combined sources
Helixi122 – 13110Combined sources
Helixi135 – 14511Combined sources
Helixi148 – 15710Combined sources
Helixi171 – 1733Combined sources
Helixi186 – 19712Combined sources
Beta strandi199 – 2024Combined sources
Helixi204 – 21512Combined sources
Turni221 – 2233Combined sources
Helixi224 – 2274Combined sources
Beta strandi232 – 24110Combined sources
Turni242 – 2443Combined sources
Beta strandi245 – 2539Combined sources
Beta strandi255 – 2573Combined sources
Beta strandi260 – 2678Combined sources
Helixi277 – 28812Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3BYDX-ray1.93A25-291[»]
ProteinModelPortaliP22391.
SMRiP22391. Positions 31-291.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22391.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni237 – 2393Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22391-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLKSSWRKTA LMAAAAVPLL LASGSLWASA DAIQQKLADL EKRSGGRLGV
60 70 80 90 100
ALINTADDSQ TLYRGDERFA MCSTGKVMAA AAVLKQSESN PEVVNKRLEI
110 120 130 140 150
KKSDLVVWSP ITEKHLQSGM TLAELSAAAL QYSDNTAMNK MISYLGGPEK
160 170 180 190 200
VTAFAQSIGD VTFRLDRTEP ALNSAIPGDK RDTTTPLAMA ESLRKLTLGN
210 220 230 240 250
ALGEQQRAQL VTWLKGNTTG GQSIRAGLPA SWAVGDKTGA GDYGTTNDIA
260 270 280 290
VIWPENHAPL VLVTYFTQPQ QDAKSRKEVL AAAAKIVTEG L
Length:291
Mass (Da):31,044
Last modified:August 1, 1991 - v1
Checksum:i9908F5AA97EDDC8A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti262 – 2621L → P in strain: KH66.
Natural varianti278 – 2781E → K in strain: KH66.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27459 Genomic DNA. Translation: AAA25084.1.
Z30177 Genomic DNA. Translation: CAA82916.1.
Z30178 Genomic DNA. Translation: CAA82917.1.
Y17715 Genomic DNA. Translation: CAB42615.1.
PIRiS42075.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27459 Genomic DNA. Translation: AAA25084.1 .
Z30177 Genomic DNA. Translation: CAA82916.1 .
Z30178 Genomic DNA. Translation: CAA82917.1 .
Y17715 Genomic DNA. Translation: CAB42615.1 .
PIRi S42075.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3BYD X-ray 1.93 A 25-291 [» ]
ProteinModelPortali P22391.
SMRi P22391. Positions 31-291.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P22391.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view ]
Pfami PF00144. Beta-lactamase. 1 hit.
[Graphical view ]
PRINTSi PR00118. BLACTAMASEA.
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Chromosomal beta-lactamase of Klebsiella oxytoca, a new class A enzyme that hydrolyzes broad-spectrum beta-lactam antibiotics."
    Arakawa Y., Ohta M., Kido N., Mori M., Ito H., Komatsu T., Fujii Y., Kato N.
    Antimicrob. Agents Chemother. 33:63-70(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: E23004.
  2. "Klebsiella oxytoca: resistance to aztreonam by overproduction of the chromosomally encoded beta-lactamase."
    Fournier B., Arlet G., Lagrange P.H., Philippon A.
    FEMS Microbiol. Lett. 116:31-36(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SL781 and SL7811.
  3. "Genetic characterization of resistance to extended-spectrum beta-lactams in Klebsiella oxytoca isolates recovered from patients with septicemia at hospitals in the Stockholm area."
    Wu S.W., Dornbusch K., Kronvall G.
    Antimicrob. Agents Chemother. 43:1294-1297(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: KH66.

Entry informationi

Entry nameiBLO1_KLEOX
AccessioniPrimary (citable) accession number: P22391
Secondary accession number(s): Q9S6S0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: November 26, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In strain KH66 OXY-1 is known as OXY-1a.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3