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P22390 (BLAC_CITKO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase

EC=3.5.2.6
Alternative name(s):
Penicillinase
OrganismCitrobacter koseri (Citrobacter diversus)
Taxonomic identifier545 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacter

Protein attributes

Sequence length294 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainSignal
   Molecular functionHydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Ref.2
Chain28 – 294267Beta-lactamase
PRO_0000016977

Regions

Region240 – 2423Substrate binding By similarity

Sites

Active site761Acyl-ester intermediate
Active site1741Proton acceptor By similarity

Experimental info

Sequence conflict281R → G AA sequence Ref.2
Sequence conflict611R → S AA sequence Ref.2
Sequence conflict80 – 834TMVA → AMAT AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
P22390 [UniParc].

Last modified December 1, 1992. Version 2.
Checksum: BF075822858C54BB

FASTA29431,862
        10         20         30         40         50         60 
MFKKRGRQTV LIAAVLAFFT ASSPLLARTQ GEPTQVQQKL AALEKQSGGR LGVALINTAD 

        70         80         90        100        110        120 
RSQILYRGDE RFAMCSTSKT MVAAAVLKQS ETQHDILQQK MVIKKADLTN WNPVTEKYVD 

       130        140        150        160        170        180 
KEMTLAELSA ATLQYSDNTA MNKLLEHLGG TSNVTAFARS IGDTTFRLDR KEPELNTAIP 

       190        200        210        220        230        240 
GDERDTTCPL AMAKSLHKLT LGDALAGAQR AQLVEWLKGN TTGGQSIRAG LPEGWVVGDK 

       250        260        270        280        290 
TGAGDYGTTN DIAVIWPEDR APLILVTYFT QPQQDAKGRK DILAAAAKIV TEGL 

« Hide

References

[1]"Cloning and nucleotide sequencing of the gene encoding the beta-lactamase from Citrobacter diversus."
Perilli M., Franceschini N., Segatore B., Amicosante G., Oratore A., Duez C., Joris B., Frere J.-M.
FEMS Microbiol. Lett. 67:79-84(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ULA-27.
[2]"Proteolytic interconversion and N-terminal sequences of the Citrobacter diversus major beta-lactamases."
Franceschini N., Amicosante G., Perilli M., Maccarrone M., Oratore A., van Beeumen J., Frere J.-M.
Biochem. J. 275:629-633(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-83.
Strain: ULA-27.
[3]"Chromosome-encoded beta-lactamases of Citrobacter diversus. Interaction with beta-iodopenicillanate and labelling of the active site."
Amicosante G., Oratore A., Joris B., Galleni M., Frere J.-M., van Beeumen J.
Biochem. J. 254:891-893(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 72-79.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X62610 Genomic DNA. Translation: CAA44485.1.
PIRS19006.

3D structure databases

ProteinModelPortalP22390.
SMRP22390. Positions 35-293.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP22390.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBLAC_CITKO
AccessionPrimary (citable) accession number: P22390
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: December 1, 1992
Last modified: December 11, 2013
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families