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Protein

Beta-lactamase

Gene
N/A
Organism
Citrobacter koseri (Citrobacter diversus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei76 – 761Acyl-ester intermediate
Active sitei174 – 1741Proton acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

SABIO-RKP22390.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Penicillinase
OrganismiCitrobacter koseri (Citrobacter diversus)
Taxonomic identifieri545 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 27271 PublicationAdd
BLAST
Chaini28 – 294267Beta-lactamasePRO_0000016977Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP22390.
SMRiP22390. Positions 35-293.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni240 – 2423Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22390-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKKRGRQTV LIAAVLAFFT ASSPLLARTQ GEPTQVQQKL AALEKQSGGR
60 70 80 90 100
LGVALINTAD RSQILYRGDE RFAMCSTSKT MVAAAVLKQS ETQHDILQQK
110 120 130 140 150
MVIKKADLTN WNPVTEKYVD KEMTLAELSA ATLQYSDNTA MNKLLEHLGG
160 170 180 190 200
TSNVTAFARS IGDTTFRLDR KEPELNTAIP GDERDTTCPL AMAKSLHKLT
210 220 230 240 250
LGDALAGAQR AQLVEWLKGN TTGGQSIRAG LPEGWVVGDK TGAGDYGTTN
260 270 280 290
DIAVIWPEDR APLILVTYFT QPQQDAKGRK DILAAAAKIV TEGL
Length:294
Mass (Da):31,862
Last modified:December 1, 1992 - v2
Checksum:iBF075822858C54BB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281R → G AA sequence (PubMed:2039443).Curated
Sequence conflicti61 – 611R → S AA sequence (PubMed:2039443).Curated
Sequence conflicti80 – 834TMVA → AMAT AA sequence (PubMed:2039443).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62610 Genomic DNA. Translation: CAA44485.1.
PIRiS19006.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62610 Genomic DNA. Translation: CAA44485.1.
PIRiS19006.

3D structure databases

ProteinModelPortaliP22390.
SMRiP22390. Positions 35-293.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP22390.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and nucleotide sequencing of the gene encoding the beta-lactamase from Citrobacter diversus."
    Perilli M., Franceschini N., Segatore B., Amicosante G., Oratore A., Duez C., Joris B., Frere J.-M.
    FEMS Microbiol. Lett. 67:79-84(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ULA-27.
  2. "Proteolytic interconversion and N-terminal sequences of the Citrobacter diversus major beta-lactamases."
    Franceschini N., Amicosante G., Perilli M., Maccarrone M., Oratore A., van Beeumen J., Frere J.-M.
    Biochem. J. 275:629-633(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-83.
    Strain: ULA-27.
  3. "Chromosome-encoded beta-lactamases of Citrobacter diversus. Interaction with beta-iodopenicillanate and labelling of the active site."
    Amicosante G., Oratore A., Joris B., Galleni M., Frere J.-M., van Beeumen J.
    Biochem. J. 254:891-893(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 72-79.

Entry informationi

Entry nameiBLAC_CITKO
AccessioniPrimary (citable) accession number: P22390
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: December 1, 1992
Last modified: June 24, 2015
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.