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P22390

- BLAC_CITKO

UniProt

P22390 - BLAC_CITKO

Protein

Beta-lactamase

Gene
N/A
Organism
Citrobacter koseri (Citrobacter diversus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 2 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei76 – 761Acyl-ester intermediate
    Active sitei174 – 1741Proton acceptorBy similarity

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Enzyme and pathway databases

    SABIO-RKP22390.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase (EC:3.5.2.6)
    Alternative name(s):
    Penicillinase
    OrganismiCitrobacter koseri (Citrobacter diversus)
    Taxonomic identifieri545 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacter

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 27271 PublicationAdd
    BLAST
    Chaini28 – 294267Beta-lactamasePRO_0000016977Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP22390.
    SMRiP22390. Positions 35-293.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni240 – 2423Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PfamiPF00144. Beta-lactamase. 1 hit.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P22390-1 [UniParc]FASTAAdd to Basket

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    MFKKRGRQTV LIAAVLAFFT ASSPLLARTQ GEPTQVQQKL AALEKQSGGR    50
    LGVALINTAD RSQILYRGDE RFAMCSTSKT MVAAAVLKQS ETQHDILQQK 100
    MVIKKADLTN WNPVTEKYVD KEMTLAELSA ATLQYSDNTA MNKLLEHLGG 150
    TSNVTAFARS IGDTTFRLDR KEPELNTAIP GDERDTTCPL AMAKSLHKLT 200
    LGDALAGAQR AQLVEWLKGN TTGGQSIRAG LPEGWVVGDK TGAGDYGTTN 250
    DIAVIWPEDR APLILVTYFT QPQQDAKGRK DILAAAAKIV TEGL 294
    Length:294
    Mass (Da):31,862
    Last modified:December 1, 1992 - v2
    Checksum:iBF075822858C54BB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti28 – 281R → G AA sequence (PubMed:2039443)Curated
    Sequence conflicti61 – 611R → S AA sequence (PubMed:2039443)Curated
    Sequence conflicti80 – 834TMVA → AMAT AA sequence (PubMed:2039443)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62610 Genomic DNA. Translation: CAA44485.1.
    PIRiS19006.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62610 Genomic DNA. Translation: CAA44485.1 .
    PIRi S19006.

    3D structure databases

    ProteinModelPortali P22390.
    SMRi P22390. Positions 35-293.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    SABIO-RK P22390.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view ]
    Pfami PF00144. Beta-lactamase. 1 hit.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and nucleotide sequencing of the gene encoding the beta-lactamase from Citrobacter diversus."
      Perilli M., Franceschini N., Segatore B., Amicosante G., Oratore A., Duez C., Joris B., Frere J.-M.
      FEMS Microbiol. Lett. 67:79-84(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ULA-27.
    2. "Proteolytic interconversion and N-terminal sequences of the Citrobacter diversus major beta-lactamases."
      Franceschini N., Amicosante G., Perilli M., Maccarrone M., Oratore A., van Beeumen J., Frere J.-M.
      Biochem. J. 275:629-633(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-83.
      Strain: ULA-27.
    3. "Chromosome-encoded beta-lactamases of Citrobacter diversus. Interaction with beta-iodopenicillanate and labelling of the active site."
      Amicosante G., Oratore A., Joris B., Galleni M., Frere J.-M., van Beeumen J.
      Biochem. J. 254:891-893(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 72-79.

    Entry informationi

    Entry nameiBLAC_CITKO
    AccessioniPrimary (citable) accession number: P22390
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 66 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3