P22390 (BLAC_CITDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Beta-lactamase EC=3.5.2.6 Alternative name(s): Penicillinase
Organism	Citrobacter diversus
Taxonomic identifier	545 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Ref.2
	Chain	28 – 294	267	Beta-lactamase		PRO_0000016977
Regions
	Region	240 – 242	3	Substrate binding By similarity
Sites
	Active site	76	1	Acyl-ester intermediate
	Active site	174	1	Proton acceptor By similarity
Experimental info
	Sequence conflict	28	1	R → G AA sequence Ref.2
	Sequence conflict	61	1	R → S AA sequence Ref.2
	Sequence conflict	80 – 83	4	TMVA → AMAT AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P22390 [UniParc]. Last modified December 1, 1992. Version 2. Checksum: BF075822858C54BB	FASTA	294	31,862
	10 20 30 40 50 60 MFKKRGRQTV LIAAVLAFFT ASSPLLARTQ GEPTQVQQKL AALEKQSGGR LGVALINTAD 70 80 90 100 110 120 RSQILYRGDE RFAMCSTSKT MVAAAVLKQS ETQHDILQQK MVIKKADLTN WNPVTEKYVD 130 140 150 160 170 180 KEMTLAELSA ATLQYSDNTA MNKLLEHLGG TSNVTAFARS IGDTTFRLDR KEPELNTAIP 190 200 210 220 230 240 GDERDTTCPL AMAKSLHKLT LGDALAGAQR AQLVEWLKGN TTGGQSIRAG LPEGWVVGDK 250 260 270 280 290 TGAGDYGTTN DIAVIWPEDR APLILVTYFT QPQQDAKGRK DILAAAAKIV TEGL

References

[1]	"Cloning and nucleotide sequencing of the gene encoding the beta-lactamase from Citrobacter diversus." Perilli M., Franceschini N., Segatore B., Amicosante G., Oratore A., Duez C., Joris B., Frere J.-M. FEMS Microbiol. Lett. 67:79-84(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ULA-27.
[2]	"Proteolytic interconversion and N-terminal sequences of the Citrobacter diversus major beta-lactamases." Franceschini N., Amicosante G., Perilli M., Maccarrone M., Oratore A., van Beeumen J., Frere J.-M. Biochem. J. 275:629-633(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-83. Strain: ULA-27.
[3]	"Chromosome-encoded beta-lactamases of Citrobacter diversus. Interaction with beta-iodopenicillanate and labelling of the active site." Amicosante G., Oratore A., Joris B., Galleni M., Frere J.-M., van Beeumen J. Biochem. J. 254:891-893(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 72-79.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62610 Genomic DNA. Translation: CAA44485.1.
PIR	S19006.
3D structure databases
ProteinModelPortal	P22390.
SMR	P22390. Positions 35-293.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
SABIO-RK	P22390.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR001466. Beta-lactam-related. IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR023650. Beta-lactam_class-A_AS. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLAC_CITDI

Accession

Primary (citable) accession number: P22390

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	December 1, 1992
Last modified:	April 3, 2013
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program