P22389 (EDN2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endothelin-2
Short name=ET-2

Alternative name(s):
Preproendothelin-2
Short name=PPET2
Vasoactive intestinal contractor
Short name=VIC

Gene names

Name:

Edn2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Vasoconstrictor.
Subcellular location	Secreted.
Sequence similarities	Belongs to the endothelin/sarafotoxin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Vasoactive Vasoconstrictor
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	artery smooth muscle contraction Inferred from electronic annotation. Source: Compara calcium-mediated signaling Inferred from electronic annotation. Source: Compara cytokine-mediated signaling pathway Inferred from electronic annotation. Source: Compara elevation of cytosolic calcium ion concentration Inferred from electronic annotation. Source: Compara inositol phosphate-mediated signaling Inferred from electronic annotation. Source: Compara macrophage activation Inferred from electronic annotation. Source: Compara macrophage chemotaxis Inferred from electronic annotation. Source: Compara neutrophil chemotaxis Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of heart rate Inferred from electronic annotation. Source: Compara positive regulation of leukocyte chemotaxis Inferred from electronic annotation. Source: Compara positive regulation of prostaglandin-endoperoxide synthase activity Inferred from mutant phenotype PubMed 9492062. Source: BHF-UCL positive regulation of smooth muscle contraction Inferred from direct assay Ref.4. Source: MGI prostaglandin biosynthetic process Inferred from direct assay PubMed 9492062. Source: BHF-UCL regulation of systemic arterial blood pressure by endothelin Inferred from electronic annotation. Source: Compara regulation of vasoconstriction Inferred from electronic annotation. Source: InterPro vein smooth muscle contraction Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular space Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 43	22			PRO_0000008099
	Peptide	46 – 66	21	Endothelin-2		PRO_0000008100
	Propeptide	67 – 175	109			PRO_0000008101
Regions
	Region	93 – 108	16	Endothelin-like
Sites
	Site	66 – 67	2	Cleavage; by KEL By similarity
Amino acid modifications
	Disulfide bond	46 ↔ 60		By similarity
	Disulfide bond	48 ↔ 56		By similarity
Experimental info
	Sequence conflict	151 – 152	2	KL → NV in CAA42155. Ref.1
	Sequence conflict	156 – 175	20	RLQPE…RWRKR → SYSQR Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P22389 [UniParc]. Last modified April 27, 2001. Version 3. Checksum: 6BCDD79CAAA0244A	FASTA	175	19,538
	10 20 30 40 50 60 MVSAWCSIAL ALLLALHEGK GQAAATLEQP ASAPKGRGPH LRFRRCSCNS WLDKECVYFC 70 80 90 100 110 120 HLDIIWVNTA GQTAPYGLGN PPRRRRRSLP KRCECSTAGD SACATFCHRR HWPEAVVAPS 130 140 150 160 170 SQAPAAVLKT GKMWTAEGDL LRKLRDISAT KLRFARLQPE VTRKAIPAYS RWRKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning, sequence analysis and tissue distribution of a precursor for vasoactive intestinal contractor (VIC)." Saida K., Mitsui Y. Biochim. Biophys. Acta 1089:404-406(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ICR.
[2]	"The prepro vasoactive intestinal contractor (VIC)/endothelin-2 gene (EDN2): structure, evolution, production, and embryonic expression." Saida K., Hashimoto M., Mitsui Y., Ishida N., Uchide T. Genomics 64:51-61(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Retina.
[4]	"A novel peptide, vasoactive intestinal contractor, of a new (endothelin) peptide family. Molecular cloning, expression, and biological activity." Saida K., Mitsui Y., Ishida N. J. Biol. Chem. 264:14613-14616(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 41-70.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59556 mRNA. Translation: CAA42155.1. AB030303 Genomic DNA. Translation: BAA94208.1. BC037042 mRNA. Translation: AAH37042.1. M26498 Genomic DNA. No translation available.
IPI	IPI00127468.
PIR	S17194.
RefSeq	NP_031928.2. NM_007902.2.
UniGene	Mm.284855.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P22389.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000030384; ENSMUSP00000030384; ENSMUSG00000028635.
GeneID	13615.
KEGG	mmu:13615.
Organism-specific databases
CTD	1907.
MGI	MGI:95284. Edn2.
Phylogenomic databases
eggNOG	NOG43933.
GeneTree	ENSGT00530000063310.
HOGENOM	HOG000231110.
HOVERGEN	HBG051441.
InParanoid	P22389.
KO	K16367.
OMA	HLRLRRC.
OrthoDB	EOG4WH8N0.
Gene expression databases
ArrayExpress	P22389.
Bgee	P22389.
CleanEx	MM_EDN2.
Genevestigator	P22389.
GermOnline	ENSMUSG00000028635. Mus musculus.
Family and domain databases
InterPro	IPR020475. Bibrotoxin/Sarafotoxin-D. IPR019764. Endothelin_toxin_CS. IPR001928. Endothln-like_toxin. IPR015658. ET-2. [Graphical view]
PANTHER	PTHR13874:SF4. PTHR13874:SF4. 1 hit.
Pfam	PF00322. Endothelin. 1 hit. [Graphical view]
PRINTS	PR00365. ENDOTHELIN.
SMART	SM00272. END. 2 hits. [Graphical view]
PROSITE	PS00270. ENDOTHELIN. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	284276.
SOURCE	Search...

Entry information

Entry name

EDN2_MOUSE

Accession

Primary (citable) accession number: P22389
Secondary accession number(s): Q9JME3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	April 27, 2001
Last modified:	April 3, 2013
This is version 116 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program