P22388 (EDN1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endothelin-1
Short name=ET-1

Alternative name(s):
Preproendothelin-1
Short name=PPET1

Gene names

Name:

Edn1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Endothelins are endothelium-derived vasoconstrictor peptides.
Subcellular location	Secreted.
Sequence similarities	Belongs to the endothelin/sarafotoxin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Vasoactive Vasoconstrictor
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	body fluid secretion Inferred from electronic annotation. Source: Compara calcium-mediated signaling Inferred from electronic annotation. Source: Compara cartilage development Inferred from electronic annotation. Source: Compara cell growth Inferred from direct assay PubMed 19635990. Source: RGD cellular response to interleukin-1 Inferred from expression pattern PubMed 20512083. Source: RGD cellular response to tumor necrosis factor Inferred from expression pattern PubMed 20512083. Source: RGD dorsal/ventral pattern formation Inferred from electronic annotation. Source: Compara elevation of cytosolic calcium ion concentration Inferred from direct assay PubMed 18288637. Source: RGD elevation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Inferred from electronic annotation. Source: Compara epithelial fluid transport Inferred from direct assay PubMed 18948426. Source: RGD glucose transport Inferred from electronic annotation. Source: Compara heart development Inferred from electronic annotation. Source: Compara histamine secretion Inferred from direct assay PubMed 18787779. Source: RGD in utero embryonic development Inferred from electronic annotation. Source: Compara inositol phosphate-mediated signaling Inferred from electronic annotation. Source: Compara intracellular protein kinase cascade Inferred from direct assay PubMed 12388423. Source: RGD maternal process involved in parturition Inferred from expression pattern PubMed 18948426. Source: RGD membrane depolarization Inferred from mutant phenotype PubMed 15655307. Source: RGD middle ear morphogenesis Inferred from electronic annotation. Source: Compara multicellular organismal aging Inferred from expression pattern PubMed 20522806. Source: RGD negative regulation of cAMP biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of cellular protein metabolic process Inferred from electronic annotation. Source: Compara negative regulation of hormone secretion Inferred from direct assay PubMed 1533364. Source: RGD negative regulation of nitric-oxide synthase biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of smooth muscle cell apoptotic process Inferred from direct assay PubMed 16857764. Source: RGD negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara neural crest cell development Inferred from electronic annotation. Source: Compara nitric oxide transport Inferred from electronic annotation. Source: Compara patterning of blood vessels Inferred from electronic annotation. Source: Compara peptide hormone secretion Inferred from electronic annotation. Source: Compara phosphatidylinositol 3-kinase cascade Inferred from electronic annotation. Source: Compara phospholipase D-activating G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of JUN kinase activity Inferred from electronic annotation. Source: Compara positive regulation of cardiac muscle hypertrophy Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from direct assay PubMed 19628632. Source: RGD positive regulation of cell size Inferred from electronic annotation. Source: Compara positive regulation of heart rate Inferred from electronic annotation. Source: Compara positive regulation of hormone secretion Inferred from electronic annotation. Source: Compara positive regulation of mitosis Inferred from electronic annotation. Source: Compara positive regulation of neutrophil chemotaxis Inferred from direct assay PubMed 18787779. Source: RGD positive regulation of odontogenesis Inferred from direct assay PubMed 16843458. Source: RGD positive regulation of prostaglandin-endoperoxide synthase activity Inferred from electronic annotation. Source: Compara positive regulation of receptor biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of renal sodium excretion Inferred from electronic annotation. Source: Compara positive regulation of sarcomere organization Inferred from electronic annotation. Source: Compara positive regulation of smooth muscle cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of smooth muscle contraction Inferred from direct assay PubMed 12396026. Source: RGD positive regulation of urine volume Inferred from electronic annotation. Source: Compara prostaglandin biosynthetic process Inferred from electronic annotation. Source: Compara protein kinase C deactivation Inferred from electronic annotation. Source: Compara protein kinase C-activating G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara regulation of blood pressure Inferred from mutant phenotype PubMed 14707164. Source: RGD regulation of pH Inferred from electronic annotation. Source: Compara regulation of sensory perception of pain Inferred from direct assay PubMed 19559389. Source: RGD regulation of systemic arterial blood pressure by endothelin Inferred from electronic annotation. Source: Compara regulation of vasoconstriction Inferred from electronic annotation. Source: InterPro respiratory gaseous exchange Inferred from electronic annotation. Source: Compara response to amino acid stimulus Inferred from expression pattern PubMed 20871618. Source: RGD response to hypoxia Inferred from expression pattern PubMed 12060564. Source: RGD response to lipopolysaccharide Inferred from expression pattern PubMed 16360659. Source: RGD response to nicotine Inferred from expression pattern PubMed 20854829. Source: RGD response to testosterone stimulus Inferred from expression pattern PubMed 20660069. Source: RGD rhythmic excitation Inferred from electronic annotation. Source: Compara sensory perception of pain Inferred from direct assay PubMed 19429182. Source: RGD superoxide anion generation Inferred from direct assay PubMed 16788136. Source: RGD vasoconstriction Inferred from direct assay PubMed 19628632. Source: RGD vein smooth muscle contraction Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara extracellular space Inferred from direct assay PubMed 15738350. Source: RGD
Molecular_function	endothelin A receptor binding Inferred from mutant phenotype PubMed 15655307. Source: RGD endothelin B receptor binding Inferred from mutant phenotype PubMed 15655307. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 25

25

Potential

Propeptide

26 – 50

25

PRO_0000008072

Peptide

53 – 73

21

Endothelin-1

PRO_0000008073

Propeptide

74 – 202

129

PRO_0000008074

Regions

Region

110 – 124

15

Endothelin-like

Sites

Site

73 – 74

2

Cleavage; by KEL By similarity

Amino acid modifications

Disulfide bond

53 ↔ 67

By similarity

Disulfide bond

55 ↔ 63

By similarity

Secondary structure

1

.

.

.

.

.

202

Helix Strand Turn

Details...

Helix

54 – 57

4

Helix

61 – 69

9

Sequences

	Sequence		Length	Mass (Da)
	P22388 [UniParc]. Last modified November 1, 1991. Version 2. Checksum: 701886BAFD09AC4B	FASTA	202	23,135
	10 20 30 40 50 60 MDYFPVIFSL LFVAFQGAPE TAVLGAELSP RAEKEVQSPP PSTSWRPRRS KRCSCSSLMD 70 80 90 100 110 120 KECVYFCHLD IIWVNTPERV VPYGLGSPSR SKRSLKDLLP TKTTDQGNRC QCAHQKDKKC 130 140 150 160 170 180 WNFCQADKEL RAQSTMQKGV KDFKKGKPCP KLGKKCIYQQ LVEGRKLRRL EAISNSIKTS 190 200 FRVAKLKAEL YRDQKLIHNR AH

References

[1]	"cDNA cloning, sequence analysis and tissue distribution of rat preproendothelin-1 mRNA." Sakurai T., Yanagisawa M., Inoue A., Ryan U.S., Kimura S., Mitsui Y., Goto K., Masaki T. Biochem. Biophys. Res. Commun. 175:44-47(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Endothelin-1 mRNA expression in the rat kidney." Nunez D.J.R., Taylor E.A., Oh V.M.S., Schofield J.P., Brown M.J. Biochem. J. 275:817-819(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 51-77. Strain: Wistar. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M64711 mRNA. Translation: AAA41129.1.

IPI

IPI00206210.

PIR

JN0125.

RefSeq

NP_036680.1. NM_012548.2.

UniGene

Rn.10918.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3CMH	NMR	-	A	60-73	[»]
6CMH	NMR	-	A	53-73	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

10116.ENSRNOP00000019361.

Proteomic databases

PRIDE

P22388.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000019361; ENSRNOP00000019361; ENSRNOG00000014361.

GeneID

24323.

KEGG

rno:24323.

UCSC

RGD:2532. rat.

Organism-specific databases

CTD

1906.

RGD

2532. Edn1.

Phylogenomic databases

eggNOG

NOG46593.

GeneTree

ENSGT00530000063310.

HOGENOM

HOG000231110.

HOVERGEN

HBG051441.

InParanoid

P22388.

KO

K16366.

OMA

RCQCASQ.

OrthoDB

EOG4QZ7N8.

Gene expression databases

ArrayExpress

P22388.

Genevestigator

P22388.

GermOnline

ENSRNOG00000014361. Rattus norvegicus.

Family and domain databases

InterPro

IPR020475. Bibrotoxin/Sarafotoxin-D.
IPR019764. Endothelin_toxin_CS.
IPR001928. Endothln-like_toxin.
[Graphical view]

Pfam

PF00322. Endothelin. 1 hit.
[Graphical view]

PRINTS

PR00365. ENDOTHELIN.

SMART

SM00272. END. 2 hits.
[Graphical view]

PROSITE

PS00270. ENDOTHELIN. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P22388.

NextBio

602983.

Entry information

Entry name

EDN1_RAT

Accession

Primary (citable) accession number: P22388

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	November 1, 1991
Last modified:	April 3, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program