Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P22388 (EDN1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endothelin-1

Short name=ET-1
Alternative name(s):
Preproendothelin-1
Short name=PPET1
Gene names
Name:Edn1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length202 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Endothelins are endothelium-derived vasoconstrictor peptides.

Subcellular location

Secreted.

Sequence similarities

Belongs to the endothelin/sarafotoxin family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionVasoactive
Vasoconstrictor
   PTMCleavage on pair of basic residues
Disulfide bond
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbody fluid secretion

Inferred from electronic annotation. Source: Ensembl

calcium-mediated signaling

Inferred from electronic annotation. Source: Ensembl

cartilage development

Inferred from electronic annotation. Source: Ensembl

cell growth

Inferred from direct assay PubMed 19635990. Source: RGD

cellular response to interleukin-1

Inferred from expression pattern PubMed 20512083. Source: RGD

cellular response to tumor necrosis factor

Inferred from expression pattern PubMed 20512083. Source: RGD

dorsal/ventral pattern formation

Inferred from electronic annotation. Source: Ensembl

epithelial fluid transport

Inferred from direct assay PubMed 18948426. Source: RGD

glucose transport

Inferred from electronic annotation. Source: Ensembl

heart development

Inferred from electronic annotation. Source: Ensembl

histamine secretion

Inferred from direct assay PubMed 18787779. Source: RGD

in utero embryonic development

Inferred from electronic annotation. Source: Ensembl

inositol phosphate-mediated signaling

Inferred from electronic annotation. Source: Ensembl

intracellular signal transduction

Inferred from direct assay PubMed 12388423. Source: RGD

maternal process involved in parturition

Inferred from expression pattern PubMed 18948426. Source: RGD

membrane depolarization

Inferred from mutant phenotype PubMed 15655307. Source: RGD

middle ear morphogenesis

Inferred from electronic annotation. Source: Ensembl

multicellular organismal aging

Inferred from expression pattern PubMed 20522806. Source: RGD

negative regulation of cAMP biosynthetic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of cellular protein metabolic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of hormone secretion

Inferred from direct assay PubMed 1533364. Source: RGD

negative regulation of nitric-oxide synthase biosynthetic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of smooth muscle cell apoptotic process

Inferred from direct assay PubMed 16857764. Source: RGD

negative regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

neural crest cell development

Inferred from electronic annotation. Source: Ensembl

nitric oxide transport

Inferred from electronic annotation. Source: Ensembl

patterning of blood vessels

Inferred from electronic annotation. Source: Ensembl

peptide hormone secretion

Inferred from electronic annotation. Source: Ensembl

phosphatidylinositol 3-kinase signaling

Inferred from electronic annotation. Source: Ensembl

phospholipase D-activating G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of JUN kinase activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of cardiac muscle hypertrophy

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation

Inferred from direct assay PubMed 19628632. Source: RGD

positive regulation of cell size

Inferred from electronic annotation. Source: Ensembl

positive regulation of cytosolic calcium ion concentration

Inferred from direct assay PubMed 18288637. Source: RGD

positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of heart rate

Inferred from electronic annotation. Source: Ensembl

positive regulation of hormone secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of mitosis

Inferred from electronic annotation. Source: Ensembl

positive regulation of neutrophil chemotaxis

Inferred from direct assay PubMed 18787779. Source: RGD

positive regulation of odontogenesis

Inferred from direct assay PubMed 16843458. Source: RGD

positive regulation of prostaglandin-endoperoxide synthase activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of receptor biosynthetic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of renal sodium excretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of sarcomere organization

Inferred from electronic annotation. Source: Ensembl

positive regulation of smooth muscle cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of smooth muscle contraction

Inferred from direct assay PubMed 12396026. Source: RGD

positive regulation of urine volume

Inferred from electronic annotation. Source: Ensembl

prostaglandin biosynthetic process

Inferred from electronic annotation. Source: Ensembl

protein kinase C deactivation

Inferred from electronic annotation. Source: Ensembl

protein kinase C-activating G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

regulation of blood pressure

Inferred from mutant phenotype PubMed 14707164. Source: RGD

regulation of pH

Inferred from electronic annotation. Source: Ensembl

regulation of sensory perception of pain

Inferred from direct assay PubMed 19559389. Source: RGD

regulation of systemic arterial blood pressure by endothelin

Inferred from electronic annotation. Source: Ensembl

regulation of vasoconstriction

Inferred from electronic annotation. Source: InterPro

respiratory gaseous exchange

Inferred from electronic annotation. Source: Ensembl

response to amino acid

Inferred from expression pattern PubMed 20871618. Source: RGD

response to drug

Inferred from expression pattern PubMed 20666571PubMed 20851640. Source: RGD

response to hypoxia

Inferred from expression pattern PubMed 12060564. Source: RGD

response to lipopolysaccharide

Inferred from expression pattern PubMed 16360659. Source: RGD

response to nicotine

Inferred from expression pattern PubMed 20854829. Source: RGD

response to testosterone

Inferred from expression pattern PubMed 20660069. Source: RGD

rhythmic excitation

Inferred from electronic annotation. Source: Ensembl

sensory perception of pain

Inferred from direct assay PubMed 19429182. Source: RGD

superoxide anion generation

Inferred from direct assay PubMed 16788136. Source: RGD

vasoconstriction

Inferred from direct assay PubMed 19628632. Source: RGD

vein smooth muscle contraction

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: Ensembl

extracellular space

Inferred from direct assay PubMed 15738350. Source: RGD

   Molecular_functionendothelin A receptor binding

Inferred from mutant phenotype PubMed 15655307. Source: RGD

endothelin B receptor binding

Inferred from mutant phenotype PubMed 15655307. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Propeptide26 – 5025
PRO_0000008072
Peptide53 – 7321Endothelin-1
PRO_0000008073
Propeptide74 – 202129
PRO_0000008074

Regions

Region110 – 12415Endothelin-like

Sites

Site73 – 742Cleavage; by KEL By similarity

Amino acid modifications

Disulfide bond53 ↔ 67 By similarity
Disulfide bond55 ↔ 63 By similarity

Secondary structure

..... 202
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P22388 [UniParc].

Last modified November 1, 1991. Version 2.
Checksum: 701886BAFD09AC4B

FASTA20223,135
        10         20         30         40         50         60 
MDYFPVIFSL LFVAFQGAPE TAVLGAELSP RAEKEVQSPP PSTSWRPRRS KRCSCSSLMD 

        70         80         90        100        110        120 
KECVYFCHLD IIWVNTPERV VPYGLGSPSR SKRSLKDLLP TKTTDQGNRC QCAHQKDKKC 

       130        140        150        160        170        180 
WNFCQADKEL RAQSTMQKGV KDFKKGKPCP KLGKKCIYQQ LVEGRKLRRL EAISNSIKTS 

       190        200 
FRVAKLKAEL YRDQKLIHNR AH 

« Hide

References

[1]"cDNA cloning, sequence analysis and tissue distribution of rat preproendothelin-1 mRNA."
Sakurai T., Yanagisawa M., Inoue A., Ryan U.S., Kimura S., Mitsui Y., Goto K., Masaki T.
Biochem. Biophys. Res. Commun. 175:44-47(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Endothelin-1 mRNA expression in the rat kidney."
Nunez D.J.R., Taylor E.A., Oh V.M.S., Schofield J.P., Brown M.J.
Biochem. J. 275:817-819(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 51-77.
Strain: Wistar.
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M64711 mRNA. Translation: AAA41129.1.
PIRJN0125.
RefSeqNP_036680.1. NM_012548.2.
XP_006253847.1. XM_006253785.1.
UniGeneRn.10918.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3CMHNMR-A60-73[»]
6CMHNMR-A53-73[»]
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000019361.

Proteomic databases

PRIDEP22388.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000019361; ENSRNOP00000019361; ENSRNOG00000014361.
GeneID24323.
KEGGrno:24323.
UCSCRGD:2532. rat.

Organism-specific databases

CTD1906.
RGD2532. Edn1.

Phylogenomic databases

eggNOGNOG46593.
GeneTreeENSGT00530000063310.
HOGENOMHOG000231110.
HOVERGENHBG051441.
InParanoidP22388.
KOK16366.
OMARCQCASQ.
OrthoDBEOG7PS1H7.
PhylomeDBP22388.
TreeFamTF333184.

Gene expression databases

ArrayExpressP22388.
GenevestigatorP22388.

Family and domain databases

InterProIPR020475. Bibrotoxin/Sarafotoxin-D.
IPR019764. Endothelin_toxin_CS.
IPR001928. Endothln-like_toxin.
[Graphical view]
PfamPF00322. Endothelin. 1 hit.
[Graphical view]
PRINTSPR00365. ENDOTHELIN.
SMARTSM00272. END. 2 hits.
[Graphical view]
PROSITEPS00270. ENDOTHELIN. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP22388.
NextBio602983.
PROP22388.

Entry information

Entry nameEDN1_RAT
AccessionPrimary (citable) accession number: P22388
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: November 1, 1991
Last modified: April 16, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references