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Protein

Endothelin-1

Gene

Edn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Endothelins are endothelium-derived vasoconstrictor peptides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei73 – 742Cleavage; by KELBy similarity

GO - Molecular functioni

  • endothelin A receptor binding Source: RGD
  • endothelin B receptor binding Source: RGD

GO - Biological processi

  • artery smooth muscle contraction Source: RGD
  • body fluid secretion Source: Ensembl
  • calcium-mediated signaling Source: Ensembl
  • cartilage development Source: Ensembl
  • cell growth Source: RGD
  • cellular response to calcium ion Source: RGD
  • cellular response to drug Source: RGD
  • cellular response to fatty acid Source: RGD
  • cellular response to glucocorticoid stimulus Source: RGD
  • cellular response to hypoxia Source: RGD
  • cellular response to interferon-gamma Source: RGD
  • cellular response to interleukin-1 Source: RGD
  • cellular response to mineralocorticoid stimulus Source: RGD
  • cellular response to peptide hormone stimulus Source: RGD
  • cellular response to transforming growth factor beta stimulus Source: RGD
  • cellular response to tumor necrosis factor Source: RGD
  • dorsal/ventral pattern formation Source: Ensembl
  • epithelial fluid transport Source: RGD
  • glucose transport Source: Ensembl
  • heart development Source: Ensembl
  • histamine secretion Source: RGD
  • inositol phosphate-mediated signaling Source: Ensembl
  • intracellular signal transduction Source: RGD
  • in utero embryonic development Source: Ensembl
  • maternal process involved in parturition Source: RGD
  • membrane depolarization Source: RGD
  • middle ear morphogenesis Source: Ensembl
  • multicellular organismal aging Source: RGD
  • negative regulation of cAMP biosynthetic process Source: Ensembl
  • negative regulation of cellular protein metabolic process Source: Ensembl
  • negative regulation of hormone secretion Source: RGD
  • negative regulation of nitric-oxide synthase biosynthetic process Source: Ensembl
  • negative regulation of smooth muscle cell apoptotic process Source: RGD
  • negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  • neural crest cell development Source: Ensembl
  • nitric oxide transport Source: Ensembl
  • patterning of blood vessels Source: Ensembl
  • peptide hormone secretion Source: Ensembl
  • phosphatidylinositol 3-kinase signaling Source: Ensembl
  • phospholipase D-activating G-protein coupled receptor signaling pathway Source: Ensembl
  • positive regulation of cardiac muscle hypertrophy Source: Ensembl
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of cell size Source: Ensembl
  • positive regulation of cytosolic calcium ion concentration Source: RGD
  • positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Source: Ensembl
  • positive regulation of heart rate Source: Ensembl
  • positive regulation of hormone secretion Source: Ensembl
  • positive regulation of JUN kinase activity Source: Ensembl
  • positive regulation of mitotic nuclear division Source: Ensembl
  • positive regulation of neutrophil chemotaxis Source: RGD
  • positive regulation of odontogenesis Source: RGD
  • positive regulation of prostaglandin-endoperoxide synthase activity Source: Ensembl
  • positive regulation of prostaglandin secretion Source: RGD
  • positive regulation of receptor biosynthetic process Source: Ensembl
  • positive regulation of renal sodium excretion Source: Ensembl
  • positive regulation of sarcomere organization Source: Ensembl
  • positive regulation of smooth muscle cell proliferation Source: Ensembl
  • positive regulation of smooth muscle contraction Source: RGD
  • positive regulation of transcription from RNA polymerase II promoter Source: RGD
  • positive regulation of urine volume Source: Ensembl
  • prostaglandin biosynthetic process Source: Ensembl
  • protein kinase C-activating G-protein coupled receptor signaling pathway Source: Ensembl
  • protein kinase C deactivation Source: Ensembl
  • regulation of blood pressure Source: RGD
  • regulation of pH Source: Ensembl
  • regulation of sensory perception of pain Source: RGD
  • regulation of systemic arterial blood pressure by endothelin Source: Ensembl
  • regulation of vasoconstriction Source: InterPro
  • respiratory gaseous exchange Source: Ensembl
  • response to activity Source: RGD
  • response to amino acid Source: RGD
  • response to dexamethasone Source: RGD
  • response to drug Source: RGD
  • response to hypoxia Source: RGD
  • response to leptin Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to muscle stretch Source: RGD
  • response to nicotine Source: RGD
  • response to ozone Source: RGD
  • response to prostaglandin F Source: RGD
  • response to salt Source: RGD
  • response to testosterone Source: RGD
  • response to transforming growth factor beta Source: RGD
  • rhythmic excitation Source: Ensembl
  • sensory perception of pain Source: RGD
  • superoxide anion generation Source: RGD
  • vasoconstriction Source: RGD
  • vein smooth muscle contraction Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Vasoactive, Vasoconstrictor

Enzyme and pathway databases

ReactomeiREACT_306851. Peptide ligand-binding receptors.
REACT_353304. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Endothelin-1
Short name:
ET-1
Alternative name(s):
Preproendothelin-1
Short name:
PPET1
Gene namesi
Name:Edn1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi2532. Edn1.

Subcellular locationi

GO - Cellular componenti

  • basal part of cell Source: RGD
  • cytosol Source: GOC
  • extracellular space Source: RGD
  • rough endoplasmic reticulum lumen Source: RGD
  • Weibel-Palade body Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Propeptidei26 – 5025PRO_0000008072Add
BLAST
Peptidei53 – 7321Endothelin-1PRO_0000008073Add
BLAST
Propeptidei74 – 202129PRO_0000008074Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi53 ↔ 67By similarity
Disulfide bondi55 ↔ 63By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PRIDEiP22388.

Expressioni

Gene expression databases

ExpressionAtlasiP22388. baseline and differential.
GenevisibleiP22388. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019361.

Structurei

Secondary structure

1
202
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi54 – 574Combined sources
Helixi61 – 699Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CMHNMR-A60-73[»]
6CMHNMR-A53-73[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22388.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni110 – 12415Endothelin-likeAdd
BLAST

Sequence similaritiesi

Belongs to the endothelin/sarafotoxin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG46593.
GeneTreeiENSGT00530000063310.
HOGENOMiHOG000231110.
HOVERGENiHBG051441.
InParanoidiP22388.
KOiK16366.
OMAiCASQKDK.
OrthoDBiEOG7PS1H7.
PhylomeDBiP22388.
TreeFamiTF333184.

Family and domain databases

InterProiIPR020475. Bibrotoxin/Sarafotoxin-D.
IPR019764. Endothelin_toxin_CS.
IPR001928. Endothln-like_toxin.
[Graphical view]
PfamiPF00322. Endothelin. 1 hit.
[Graphical view]
PRINTSiPR00365. ENDOTHELIN.
SMARTiSM00272. END. 2 hits.
[Graphical view]
PROSITEiPS00270. ENDOTHELIN. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22388-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYFPVIFSL LFVAFQGAPE TAVLGAELSP RAEKEVQSPP PSTSWRPRRS
60 70 80 90 100
KRCSCSSLMD KECVYFCHLD IIWVNTPERV VPYGLGSPSR SKRSLKDLLP
110 120 130 140 150
TKTTDQGNRC QCAHQKDKKC WNFCQADKEL RAQSTMQKGV KDFKKGKPCP
160 170 180 190 200
KLGKKCIYQQ LVEGRKLRRL EAISNSIKTS FRVAKLKAEL YRDQKLIHNR

AH
Length:202
Mass (Da):23,135
Last modified:November 1, 1991 - v2
Checksum:i701886BAFD09AC4B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64711 mRNA. Translation: AAA41129.1.
PIRiJN0125.
RefSeqiNP_036680.1. NM_012548.2.
UniGeneiRn.10918.

Genome annotation databases

EnsembliENSRNOT00000019361; ENSRNOP00000019361; ENSRNOG00000014361.
GeneIDi24323.
KEGGirno:24323.
UCSCiRGD:2532. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64711 mRNA. Translation: AAA41129.1.
PIRiJN0125.
RefSeqiNP_036680.1. NM_012548.2.
UniGeneiRn.10918.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CMHNMR-A60-73[»]
6CMHNMR-A53-73[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019361.

Proteomic databases

PRIDEiP22388.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019361; ENSRNOP00000019361; ENSRNOG00000014361.
GeneIDi24323.
KEGGirno:24323.
UCSCiRGD:2532. rat.

Organism-specific databases

CTDi1906.
RGDi2532. Edn1.

Phylogenomic databases

eggNOGiNOG46593.
GeneTreeiENSGT00530000063310.
HOGENOMiHOG000231110.
HOVERGENiHBG051441.
InParanoidiP22388.
KOiK16366.
OMAiCASQKDK.
OrthoDBiEOG7PS1H7.
PhylomeDBiP22388.
TreeFamiTF333184.

Enzyme and pathway databases

ReactomeiREACT_306851. Peptide ligand-binding receptors.
REACT_353304. G alpha (q) signalling events.

Miscellaneous databases

EvolutionaryTraceiP22388.
NextBioi602983.
PROiP22388.

Gene expression databases

ExpressionAtlasiP22388. baseline and differential.
GenevisibleiP22388. RN.

Family and domain databases

InterProiIPR020475. Bibrotoxin/Sarafotoxin-D.
IPR019764. Endothelin_toxin_CS.
IPR001928. Endothln-like_toxin.
[Graphical view]
PfamiPF00322. Endothelin. 1 hit.
[Graphical view]
PRINTSiPR00365. ENDOTHELIN.
SMARTiSM00272. END. 2 hits.
[Graphical view]
PROSITEiPS00270. ENDOTHELIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA cloning, sequence analysis and tissue distribution of rat preproendothelin-1 mRNA."
    Sakurai T., Yanagisawa M., Inoue A., Ryan U.S., Kimura S., Mitsui Y., Goto K., Masaki T.
    Biochem. Biophys. Res. Commun. 175:44-47(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Endothelin-1 mRNA expression in the rat kidney."
    Nunez D.J.R., Taylor E.A., Oh V.M.S., Schofield J.P., Brown M.J.
    Biochem. J. 275:817-819(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 51-77.
    Strain: Wistar.
    Tissue: Kidney.

Entry informationi

Entry nameiEDN1_RAT
AccessioniPrimary (citable) accession number: P22388
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: November 1, 1991
Last modified: July 22, 2015
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.