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P22387

- EDN1_MOUSE

UniProt

P22387 - EDN1_MOUSE

Protein

Endothelin-1

Gene

Edn1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 3 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Endothelins are endothelium-derived vasoconstrictor peptides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei73 – 742Cleavage; by KELBy similarity

    GO - Molecular functioni

    1. endothelin A receptor binding Source: MGI
    2. protein binding Source: MGI
    3. receptor binding Source: MGI

    GO - Biological processi

    1. blood vessel morphogenesis Source: MGI
    2. body fluid secretion Source: MGI
    3. calcium-mediated signaling Source: Ensembl
    4. cartilage development Source: MGI
    5. cell growth Source: Ensembl
    6. cellular response to interleukin-1 Source: Ensembl
    7. cellular response to tumor necrosis factor Source: Ensembl
    8. dorsal/ventral pattern formation Source: MGI
    9. epithelial fluid transport Source: Ensembl
    10. glucose transport Source: MGI
    11. G-protein coupled receptor signaling pathway Source: MGI
    12. heart development Source: MGI
    13. histamine secretion Source: Ensembl
    14. inositol phosphate-mediated signaling Source: Ensembl
    15. in utero embryonic development Source: MGI
    16. maternal process involved in parturition Source: Ensembl
    17. membrane depolarization Source: Ensembl
    18. middle ear morphogenesis Source: MGI
    19. multicellular organismal aging Source: Ensembl
    20. negative regulation of cAMP biosynthetic process Source: MGI
    21. negative regulation of cellular protein metabolic process Source: Ensembl
    22. negative regulation of hormone secretion Source: Ensembl
    23. negative regulation of nitric-oxide synthase biosynthetic process Source: Ensembl
    24. negative regulation of smooth muscle cell apoptotic process Source: Ensembl
    25. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
    26. neural crest cell development Source: MGI
    27. nitric oxide transport Source: Ensembl
    28. patterning of blood vessels Source: MGI
    29. peptide hormone secretion Source: Ensembl
    30. phosphatidylinositol 3-kinase signaling Source: Ensembl
    31. phospholipase D-activating G-protein coupled receptor signaling pathway Source: MGI
    32. positive regulation of cardiac muscle hypertrophy Source: Ensembl
    33. positive regulation of cell proliferation Source: MGI
    34. positive regulation of cell size Source: Ensembl
    35. positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Source: MGI
    36. positive regulation of heart rate Source: Ensembl
    37. positive regulation of hormone secretion Source: Ensembl
    38. positive regulation of JUN kinase activity Source: Ensembl
    39. positive regulation of mitosis Source: Ensembl
    40. positive regulation of neutrophil chemotaxis Source: Ensembl
    41. positive regulation of odontogenesis Source: Ensembl
    42. positive regulation of prostaglandin-endoperoxide synthase activity Source: Ensembl
    43. positive regulation of receptor biosynthetic process Source: Ensembl
    44. positive regulation of renal sodium excretion Source: MGI
    45. positive regulation of sarcomere organization Source: Ensembl
    46. positive regulation of smooth muscle cell proliferation Source: Ensembl
    47. positive regulation of smooth muscle contraction Source: Ensembl
    48. positive regulation of urine volume Source: MGI
    49. prostaglandin biosynthetic process Source: Ensembl
    50. protein kinase C-activating G-protein coupled receptor signaling pathway Source: MGI
    51. protein kinase C deactivation Source: Ensembl
    52. regulation of blood pressure Source: MGI
    53. regulation of pH Source: MGI
    54. regulation of sensory perception of pain Source: Ensembl
    55. regulation of systemic arterial blood pressure by endothelin Source: Ensembl
    56. regulation of vasoconstriction Source: InterPro
    57. respiratory gaseous exchange Source: MGI
    58. response to amino acid Source: Ensembl
    59. response to drug Source: Ensembl
    60. response to hypoxia Source: MGI
    61. response to lipopolysaccharide Source: Ensembl
    62. response to nicotine Source: Ensembl
    63. response to testosterone Source: Ensembl
    64. rhythmic excitation Source: MGI
    65. sensory perception of pain Source: Ensembl
    66. skeletal system development Source: MGI
    67. superoxide anion generation Source: Ensembl
    68. vein smooth muscle contraction Source: Ensembl

    Keywords - Molecular functioni

    Vasoactive, Vasoconstrictor

    Enzyme and pathway databases

    ReactomeiREACT_207651. G alpha (q) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endothelin-1
    Short name:
    ET-1
    Alternative name(s):
    Preproendothelin-1
    Short name:
    PPET1
    Gene namesi
    Name:Edn1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:95283. Edn1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. extracellular space Source: MGI

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Propeptidei26 – 5025PRO_0000008062Add
    BLAST
    Peptidei53 – 7321Endothelin-1PRO_0000008063Add
    BLAST
    Propeptidei74 – 202129PRO_0000008064Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi53 ↔ 67By similarity
    Disulfide bondi55 ↔ 63By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond

    Proteomic databases

    PRIDEiP22387.

    PTM databases

    PhosphoSiteiP22387.

    Expressioni

    Tissue specificityi

    Highest expression in the adult is in lung. Lower levels found in heart, kidney, brain and intestine. In the embryo, expressed in outer and inner pharyngeal arch surfaces. Also expressed in endothelium of dorsal aorta and arch arteries, and in epithelium of pharyngeal pouches.1 Publication

    Developmental stagei

    Expression in lung is low at 17.5 dpc fetal mice and increases progressively into adulthood.1 Publication

    Gene expression databases

    ArrayExpressiP22387.
    BgeeiP22387.
    CleanExiMM_EDN1.
    GenevestigatoriP22387.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni110 – 12415Endothelin-likeAdd
    BLAST

    Sequence similaritiesi

    Belongs to the endothelin/sarafotoxin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG46593.
    HOGENOMiHOG000231110.
    HOVERGENiHBG051441.
    InParanoidiP22387.
    KOiK16366.
    OMAiMDYFPMI.
    OrthoDBiEOG7PS1H7.
    PhylomeDBiP22387.
    TreeFamiTF333184.

    Family and domain databases

    InterProiIPR020475. Bibrotoxin/Sarafotoxin-D.
    IPR019764. Endothelin_toxin_CS.
    IPR001928. Endothln-like_toxin.
    [Graphical view]
    PfamiPF00322. Endothelin. 1 hit.
    [Graphical view]
    PRINTSiPR00365. ENDOTHELIN.
    SMARTiSM00272. END. 2 hits.
    [Graphical view]
    PROSITEiPS00270. ENDOTHELIN. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P22387-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYFPVIFSL LFVTFQGAPE TAVLGAELST GAENGVQSPP PSTPWRPRRS    50
    KRCSCSSLMD KECVYFCHLD IIWVNTPERV VPYGLGGSSR SKRSLKDLLP 100
    NKATDQAVRC QCAHQKDKKC WNFCQAGKEL RAQSTMQKSL KDSKKGKPCS 150
    KLGKKCIYQQ LVEGRKLRRL EAISNSIKAS FRVAKLKAEL YRDQKLTHNR 200
    AH 202
    Length:202
    Mass (Da):22,809
    Last modified:October 1, 1996 - v3
    Checksum:i3A0C1E1DFA797411
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti75 – 751N → K in BAA11109. (PubMed:8824799)Curated
    Sequence conflicti103 – 1031A → G in BAA07830. (PubMed:8688455)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U35233 mRNA. Translation: AAA97608.1.
    D43775 mRNA. Translation: BAA07830.1.
    D70842 Genomic DNA. Translation: BAA11109.1.
    BC029547 mRNA. Translation: AAH29547.1.
    U07982 Genomic DNA. Translation: AAA74439.1. Sequence problems.
    S66650 mRNA. Translation: AAP13983.1.
    M26497 Genomic DNA. No translation available.
    CCDSiCCDS26474.1.
    PIRiS63538.
    RefSeqiNP_034234.1. NM_010104.3.
    UniGeneiMm.14543.

    Genome annotation databases

    EnsembliENSMUST00000021796; ENSMUSP00000021796; ENSMUSG00000021367.
    GeneIDi13614.
    KEGGimmu:13614.
    UCSCiuc007qfl.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U35233 mRNA. Translation: AAA97608.1 .
    D43775 mRNA. Translation: BAA07830.1 .
    D70842 Genomic DNA. Translation: BAA11109.1 .
    BC029547 mRNA. Translation: AAH29547.1 .
    U07982 Genomic DNA. Translation: AAA74439.1 . Sequence problems.
    S66650 mRNA. Translation: AAP13983.1 .
    M26497 Genomic DNA. No translation available.
    CCDSi CCDS26474.1.
    PIRi S63538.
    RefSeqi NP_034234.1. NM_010104.3.
    UniGenei Mm.14543.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P22387.

    Proteomic databases

    PRIDEi P22387.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021796 ; ENSMUSP00000021796 ; ENSMUSG00000021367 .
    GeneIDi 13614.
    KEGGi mmu:13614.
    UCSCi uc007qfl.2. mouse.

    Organism-specific databases

    CTDi 1906.
    MGIi MGI:95283. Edn1.

    Phylogenomic databases

    eggNOGi NOG46593.
    HOGENOMi HOG000231110.
    HOVERGENi HBG051441.
    InParanoidi P22387.
    KOi K16366.
    OMAi MDYFPMI.
    OrthoDBi EOG7PS1H7.
    PhylomeDBi P22387.
    TreeFami TF333184.

    Enzyme and pathway databases

    Reactomei REACT_207651. G alpha (q) signalling events.

    Miscellaneous databases

    ChiTaRSi EDN1. mouse.
    NextBioi 284272.
    PROi P22387.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P22387.
    Bgeei P22387.
    CleanExi MM_EDN1.
    Genevestigatori P22387.

    Family and domain databases

    InterProi IPR020475. Bibrotoxin/Sarafotoxin-D.
    IPR019764. Endothelin_toxin_CS.
    IPR001928. Endothln-like_toxin.
    [Graphical view ]
    Pfami PF00322. Endothelin. 1 hit.
    [Graphical view ]
    PRINTSi PR00365. ENDOTHELIN.
    SMARTi SM00272. END. 2 hits.
    [Graphical view ]
    PROSITEi PS00270. ENDOTHELIN. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse preproendothelin-1 gene. cDNA cloning, sequence analysis and determination of sites of expression during embryonic development."
      Chan T.S., Lin C.X., Chan W.Y., Chung S.S., Chung S.K.
      Eur. J. Biochem. 234:819-826(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6 X CBA.
      Tissue: Lung.
    2. "Sequence and neuronal expression of mouse endothelin-1 cDNA."
      Kurama M., Ishida N., Matsui M., Saida K., Mitsui Y.
      Biochim. Biophys. Acta 1307:249-253(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Lung.
    3. "Sequence analysis, chromosomal location, and developmental expression of the mouse preproendothelin-1 gene."
      Maemura K., Kurihara H., Kurihara Y., Oda H., Ishikawa T., Copeland N.G., Gilbert D.J., Jenkins N.A., Yazaki Y.
      Genomics 31:177-184(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: BALB/c.
      Tissue: Liver.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N-3.
      Tissue: Mammary gland.
    5. "Targeting gene expression to the vascular wall in transgenic mice using the murine preproendothelin-1 promoter."
      Harats D., Kurihara H., Belloni P., Oakley H., Ziober A., Ackley D., Cain G., Kurihara Y., Lawn R., Sigal E.
      J. Clin. Invest. 95:1335-1344(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
      Strain: BALB/c.
    6. "Endothelin-1 mRNA in glomerular and epithelial cells of kidney."
      Chen M., Todd-Turla K., Wang W.H., Cao X., Smart A., Brosius F.C., Killen P.D., Keiser J.A., Briggs J.P., Schnermann J.
      Am. J. Physiol. 265:F542-F550(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-76.
      Tissue: Kidney.
    7. "A novel peptide, vasoactive intestinal contractor, of a new (endothelin) peptide family. Molecular cloning, expression, and biological activity."
      Saida K., Mitsui Y., Ishida N.
      J. Biol. Chem. 264:14613-14616(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 48-77.
    8. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 155-165, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.

    Entry informationi

    Entry nameiEDN1_MOUSE
    AccessioniPrimary (citable) accession number: P22387
    Secondary accession number(s): P70203, P97338
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 131 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3