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Protein

Endothelin-1

Gene

Edn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Endothelins are endothelium-derived vasoconstrictor peptides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei73 – 742Cleavage; by KELBy similarity

GO - Molecular functioni

  • cytokine activity Source: MGI
  • endothelin A receptor binding Source: MGI
  • endothelin B receptor binding Source: MGI
  • hormone activity Source: MGI
  • receptor binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Vasoactive, Vasoconstrictor

Enzyme and pathway databases

ReactomeiREACT_319210. Peptide ligand-binding receptors.
REACT_347934. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Endothelin-1
Short name:
ET-1
Alternative name(s):
Preproendothelin-1
Short name:
PPET1
Gene namesi
Name:Edn1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:95283. Edn1.

Subcellular locationi

GO - Cellular componenti

  • basal part of cell Source: Ensembl
  • cytoplasm Source: MGI
  • cytosol Source: GOC
  • extracellular space Source: MGI
  • rough endoplasmic reticulum lumen Source: Ensembl
  • Weibel-Palade body Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Propeptidei26 – 5025PRO_0000008062Add
BLAST
Peptidei53 – 7321Endothelin-1PRO_0000008063Add
BLAST
Propeptidei74 – 202129PRO_0000008064Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi53 ↔ 67By similarity
Disulfide bondi55 ↔ 63By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PRIDEiP22387.

PTM databases

PhosphoSiteiP22387.

Expressioni

Tissue specificityi

Highest expression in the adult is in lung. Lower levels found in heart, kidney, brain and intestine. In the embryo, expressed in outer and inner pharyngeal arch surfaces. Also expressed in endothelium of dorsal aorta and arch arteries, and in epithelium of pharyngeal pouches.1 Publication

Developmental stagei

Expression in lung is low at 17.5 dpc fetal mice and increases progressively into adulthood.1 Publication

Gene expression databases

BgeeiP22387.
CleanExiMM_EDN1.
ExpressionAtlasiP22387. baseline and differential.
GenevisibleiP22387. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021796.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni110 – 12415Endothelin-likeAdd
BLAST

Sequence similaritiesi

Belongs to the endothelin/sarafotoxin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG46593.
HOGENOMiHOG000231110.
HOVERGENiHBG051441.
InParanoidiP22387.
KOiK16366.
OMAiRCQCASQ.
OrthoDBiEOG7PS1H7.
PhylomeDBiP22387.
TreeFamiTF333184.

Family and domain databases

InterProiIPR020475. Bibrotoxin/Sarafotoxin-D.
IPR019764. Endothelin_toxin_CS.
IPR001928. Endothln-like_toxin.
[Graphical view]
PfamiPF00322. Endothelin. 1 hit.
[Graphical view]
PRINTSiPR00365. ENDOTHELIN.
SMARTiSM00272. END. 2 hits.
[Graphical view]
PROSITEiPS00270. ENDOTHELIN. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22387-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYFPVIFSL LFVTFQGAPE TAVLGAELST GAENGVQSPP PSTPWRPRRS
60 70 80 90 100
KRCSCSSLMD KECVYFCHLD IIWVNTPERV VPYGLGGSSR SKRSLKDLLP
110 120 130 140 150
NKATDQAVRC QCAHQKDKKC WNFCQAGKEL RAQSTMQKSL KDSKKGKPCS
160 170 180 190 200
KLGKKCIYQQ LVEGRKLRRL EAISNSIKAS FRVAKLKAEL YRDQKLTHNR

AH
Length:202
Mass (Da):22,809
Last modified:October 1, 1996 - v3
Checksum:i3A0C1E1DFA797411
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti75 – 751N → K in BAA11109 (PubMed:8824799).Curated
Sequence conflicti103 – 1031A → G in BAA07830 (PubMed:8688455).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35233 mRNA. Translation: AAA97608.1.
D43775 mRNA. Translation: BAA07830.1.
D70842 Genomic DNA. Translation: BAA11109.1.
BC029547 mRNA. Translation: AAH29547.1.
U07982 Genomic DNA. Translation: AAA74439.1. Sequence problems.
S66650 mRNA. Translation: AAP13983.1.
M26497 Genomic DNA. No translation available.
CCDSiCCDS26474.1.
PIRiS63538.
RefSeqiNP_034234.1. NM_010104.3.
UniGeneiMm.14543.

Genome annotation databases

EnsembliENSMUST00000021796; ENSMUSP00000021796; ENSMUSG00000021367.
GeneIDi13614.
KEGGimmu:13614.
UCSCiuc007qfl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35233 mRNA. Translation: AAA97608.1.
D43775 mRNA. Translation: BAA07830.1.
D70842 Genomic DNA. Translation: BAA11109.1.
BC029547 mRNA. Translation: AAH29547.1.
U07982 Genomic DNA. Translation: AAA74439.1. Sequence problems.
S66650 mRNA. Translation: AAP13983.1.
M26497 Genomic DNA. No translation available.
CCDSiCCDS26474.1.
PIRiS63538.
RefSeqiNP_034234.1. NM_010104.3.
UniGeneiMm.14543.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021796.

PTM databases

PhosphoSiteiP22387.

Proteomic databases

PRIDEiP22387.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021796; ENSMUSP00000021796; ENSMUSG00000021367.
GeneIDi13614.
KEGGimmu:13614.
UCSCiuc007qfl.2. mouse.

Organism-specific databases

CTDi1906.
MGIiMGI:95283. Edn1.

Phylogenomic databases

eggNOGiNOG46593.
HOGENOMiHOG000231110.
HOVERGENiHBG051441.
InParanoidiP22387.
KOiK16366.
OMAiRCQCASQ.
OrthoDBiEOG7PS1H7.
PhylomeDBiP22387.
TreeFamiTF333184.

Enzyme and pathway databases

ReactomeiREACT_319210. Peptide ligand-binding receptors.
REACT_347934. G alpha (q) signalling events.

Miscellaneous databases

ChiTaRSiEdn1. mouse.
NextBioi284272.
PROiP22387.
SOURCEiSearch...

Gene expression databases

BgeeiP22387.
CleanExiMM_EDN1.
ExpressionAtlasiP22387. baseline and differential.
GenevisibleiP22387. MM.

Family and domain databases

InterProiIPR020475. Bibrotoxin/Sarafotoxin-D.
IPR019764. Endothelin_toxin_CS.
IPR001928. Endothln-like_toxin.
[Graphical view]
PfamiPF00322. Endothelin. 1 hit.
[Graphical view]
PRINTSiPR00365. ENDOTHELIN.
SMARTiSM00272. END. 2 hits.
[Graphical view]
PROSITEiPS00270. ENDOTHELIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse preproendothelin-1 gene. cDNA cloning, sequence analysis and determination of sites of expression during embryonic development."
    Chan T.S., Lin C.X., Chan W.Y., Chung S.S., Chung S.K.
    Eur. J. Biochem. 234:819-826(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X CBA.
    Tissue: Lung.
  2. "Sequence and neuronal expression of mouse endothelin-1 cDNA."
    Kurama M., Ishida N., Matsui M., Saida K., Mitsui Y.
    Biochim. Biophys. Acta 1307:249-253(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Lung.
  3. "Sequence analysis, chromosomal location, and developmental expression of the mouse preproendothelin-1 gene."
    Maemura K., Kurihara H., Kurihara Y., Oda H., Ishikawa T., Copeland N.G., Gilbert D.J., Jenkins N.A., Yazaki Y.
    Genomics 31:177-184(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: BALB/c.
    Tissue: Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N-3.
    Tissue: Mammary gland.
  5. "Targeting gene expression to the vascular wall in transgenic mice using the murine preproendothelin-1 promoter."
    Harats D., Kurihara H., Belloni P., Oakley H., Ziober A., Ackley D., Cain G., Kurihara Y., Lawn R., Sigal E.
    J. Clin. Invest. 95:1335-1344(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
    Strain: BALB/c.
  6. "Endothelin-1 mRNA in glomerular and epithelial cells of kidney."
    Chen M., Todd-Turla K., Wang W.H., Cao X., Smart A., Brosius F.C., Killen P.D., Keiser J.A., Briggs J.P., Schnermann J.
    Am. J. Physiol. 265:F542-F550(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-76.
    Tissue: Kidney.
  7. "A novel peptide, vasoactive intestinal contractor, of a new (endothelin) peptide family. Molecular cloning, expression, and biological activity."
    Saida K., Mitsui Y., Ishida N.
    J. Biol. Chem. 264:14613-14616(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 48-77.
  8. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 155-165, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.

Entry informationi

Entry nameiEDN1_MOUSE
AccessioniPrimary (citable) accession number: P22387
Secondary accession number(s): P70203, P97338
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: October 1, 1996
Last modified: June 24, 2015
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.