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P22387

- EDN1_MOUSE

UniProt

P22387 - EDN1_MOUSE

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Protein

Endothelin-1

Gene

Edn1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Endothelins are endothelium-derived vasoconstrictor peptides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei73 – 742Cleavage; by KELBy similarity

GO - Molecular functioni

  1. endothelin A receptor binding Source: MGI
  2. receptor binding Source: MGI

GO - Biological processi

  1. artery smooth muscle contraction Source: Ensembl
  2. blood vessel morphogenesis Source: MGI
  3. body fluid secretion Source: MGI
  4. calcium-mediated signaling Source: Ensembl
  5. cartilage development Source: MGI
  6. cell growth Source: Ensembl
  7. cellular response to calcium ion Source: Ensembl
  8. cellular response to drug Source: Ensembl
  9. cellular response to fatty acid Source: Ensembl
  10. cellular response to glucocorticoid stimulus Source: Ensembl
  11. cellular response to hypoxia Source: Ensembl
  12. cellular response to interferon-gamma Source: Ensembl
  13. cellular response to interleukin-1 Source: Ensembl
  14. cellular response to mineralocorticoid stimulus Source: Ensembl
  15. cellular response to peptide hormone stimulus Source: Ensembl
  16. cellular response to transforming growth factor beta stimulus Source: Ensembl
  17. cellular response to tumor necrosis factor Source: Ensembl
  18. dorsal/ventral pattern formation Source: MGI
  19. epithelial fluid transport Source: Ensembl
  20. glucose transport Source: MGI
  21. G-protein coupled receptor signaling pathway Source: MGI
  22. heart development Source: MGI
  23. histamine secretion Source: Ensembl
  24. inositol phosphate-mediated signaling Source: Ensembl
  25. in utero embryonic development Source: MGI
  26. maternal process involved in parturition Source: Ensembl
  27. membrane depolarization Source: Ensembl
  28. middle ear morphogenesis Source: MGI
  29. multicellular organismal aging Source: Ensembl
  30. negative regulation of cAMP biosynthetic process Source: MGI
  31. negative regulation of cellular protein metabolic process Source: Ensembl
  32. negative regulation of hormone secretion Source: Ensembl
  33. negative regulation of nitric-oxide synthase biosynthetic process Source: Ensembl
  34. negative regulation of smooth muscle cell apoptotic process Source: Ensembl
  35. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  36. neural crest cell development Source: MGI
  37. nitric oxide transport Source: Ensembl
  38. patterning of blood vessels Source: MGI
  39. peptide hormone secretion Source: Ensembl
  40. phosphatidylinositol 3-kinase signaling Source: Ensembl
  41. phospholipase D-activating G-protein coupled receptor signaling pathway Source: MGI
  42. positive regulation of cardiac muscle hypertrophy Source: Ensembl
  43. positive regulation of cell proliferation Source: MGI
  44. positive regulation of cell size Source: Ensembl
  45. positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Source: MGI
  46. positive regulation of heart rate Source: Ensembl
  47. positive regulation of hormone secretion Source: Ensembl
  48. positive regulation of JUN kinase activity Source: Ensembl
  49. positive regulation of mitosis Source: Ensembl
  50. positive regulation of neutrophil chemotaxis Source: Ensembl
  51. positive regulation of odontogenesis Source: Ensembl
  52. positive regulation of prostaglandin-endoperoxide synthase activity Source: Ensembl
  53. positive regulation of prostaglandin secretion Source: Ensembl
  54. positive regulation of receptor biosynthetic process Source: Ensembl
  55. positive regulation of renal sodium excretion Source: MGI
  56. positive regulation of sarcomere organization Source: Ensembl
  57. positive regulation of smooth muscle cell proliferation Source: Ensembl
  58. positive regulation of smooth muscle contraction Source: Ensembl
  59. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  60. positive regulation of urine volume Source: MGI
  61. prostaglandin biosynthetic process Source: Ensembl
  62. protein kinase C-activating G-protein coupled receptor signaling pathway Source: MGI
  63. protein kinase C deactivation Source: Ensembl
  64. regulation of blood pressure Source: MGI
  65. regulation of pH Source: MGI
  66. regulation of sensory perception of pain Source: Ensembl
  67. regulation of systemic arterial blood pressure by endothelin Source: Ensembl
  68. regulation of vasoconstriction Source: InterPro
  69. respiratory gaseous exchange Source: MGI
  70. response to activity Source: Ensembl
  71. response to amino acid Source: Ensembl
  72. response to dexamethasone Source: Ensembl
  73. response to hypoxia Source: MGI
  74. response to leptin Source: Ensembl
  75. response to lipopolysaccharide Source: Ensembl
  76. response to muscle stretch Source: Ensembl
  77. response to nicotine Source: Ensembl
  78. response to ozone Source: Ensembl
  79. response to prostaglandin F Source: Ensembl
  80. response to salt Source: Ensembl
  81. response to testosterone Source: Ensembl
  82. rhythmic excitation Source: MGI
  83. sensory perception of pain Source: Ensembl
  84. skeletal system development Source: MGI
  85. superoxide anion generation Source: Ensembl
  86. vein smooth muscle contraction Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Vasoactive, Vasoconstrictor

Enzyme and pathway databases

ReactomeiREACT_207651. G alpha (q) signalling events.
REACT_235286. Peptide ligand-binding receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Endothelin-1
Short name:
ET-1
Alternative name(s):
Preproendothelin-1
Short name:
PPET1
Gene namesi
Name:Edn1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:95283. Edn1.

Subcellular locationi

GO - Cellular componenti

  1. basal part of cell Source: Ensembl
  2. extracellular space Source: MGI
  3. rough endoplasmic reticulum lumen Source: Ensembl
  4. Weibel-Palade body Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Propeptidei26 – 5025PRO_0000008062Add
BLAST
Peptidei53 – 7321Endothelin-1PRO_0000008063Add
BLAST
Propeptidei74 – 202129PRO_0000008064Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi53 ↔ 67By similarity
Disulfide bondi55 ↔ 63By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PRIDEiP22387.

PTM databases

PhosphoSiteiP22387.

Expressioni

Tissue specificityi

Highest expression in the adult is in lung. Lower levels found in heart, kidney, brain and intestine. In the embryo, expressed in outer and inner pharyngeal arch surfaces. Also expressed in endothelium of dorsal aorta and arch arteries, and in epithelium of pharyngeal pouches.1 Publication

Developmental stagei

Expression in lung is low at 17.5 dpc fetal mice and increases progressively into adulthood.1 Publication

Gene expression databases

BgeeiP22387.
CleanExiMM_EDN1.
ExpressionAtlasiP22387. baseline and differential.
GenevestigatoriP22387.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni110 – 12415Endothelin-likeAdd
BLAST

Sequence similaritiesi

Belongs to the endothelin/sarafotoxin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG46593.
HOGENOMiHOG000231110.
HOVERGENiHBG051441.
InParanoidiP22387.
KOiK16366.
OMAiRCQCASQ.
OrthoDBiEOG7PS1H7.
PhylomeDBiP22387.
TreeFamiTF333184.

Family and domain databases

InterProiIPR020475. Bibrotoxin/Sarafotoxin-D.
IPR019764. Endothelin_toxin_CS.
IPR001928. Endothln-like_toxin.
[Graphical view]
PfamiPF00322. Endothelin. 1 hit.
[Graphical view]
PRINTSiPR00365. ENDOTHELIN.
SMARTiSM00272. END. 2 hits.
[Graphical view]
PROSITEiPS00270. ENDOTHELIN. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22387-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYFPVIFSL LFVTFQGAPE TAVLGAELST GAENGVQSPP PSTPWRPRRS
60 70 80 90 100
KRCSCSSLMD KECVYFCHLD IIWVNTPERV VPYGLGGSSR SKRSLKDLLP
110 120 130 140 150
NKATDQAVRC QCAHQKDKKC WNFCQAGKEL RAQSTMQKSL KDSKKGKPCS
160 170 180 190 200
KLGKKCIYQQ LVEGRKLRRL EAISNSIKAS FRVAKLKAEL YRDQKLTHNR

AH
Length:202
Mass (Da):22,809
Last modified:October 1, 1996 - v3
Checksum:i3A0C1E1DFA797411
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti75 – 751N → K in BAA11109. (PubMed:8824799)Curated
Sequence conflicti103 – 1031A → G in BAA07830. (PubMed:8688455)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35233 mRNA. Translation: AAA97608.1.
D43775 mRNA. Translation: BAA07830.1.
D70842 Genomic DNA. Translation: BAA11109.1.
BC029547 mRNA. Translation: AAH29547.1.
U07982 Genomic DNA. Translation: AAA74439.1. Sequence problems.
S66650 mRNA. Translation: AAP13983.1.
M26497 Genomic DNA. No translation available.
CCDSiCCDS26474.1.
PIRiS63538.
RefSeqiNP_034234.1. NM_010104.3.
UniGeneiMm.14543.

Genome annotation databases

EnsembliENSMUST00000021796; ENSMUSP00000021796; ENSMUSG00000021367.
GeneIDi13614.
KEGGimmu:13614.
UCSCiuc007qfl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35233 mRNA. Translation: AAA97608.1 .
D43775 mRNA. Translation: BAA07830.1 .
D70842 Genomic DNA. Translation: BAA11109.1 .
BC029547 mRNA. Translation: AAH29547.1 .
U07982 Genomic DNA. Translation: AAA74439.1 . Sequence problems.
S66650 mRNA. Translation: AAP13983.1 .
M26497 Genomic DNA. No translation available.
CCDSi CCDS26474.1.
PIRi S63538.
RefSeqi NP_034234.1. NM_010104.3.
UniGenei Mm.14543.

3D structure databases

ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P22387.

Proteomic databases

PRIDEi P22387.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021796 ; ENSMUSP00000021796 ; ENSMUSG00000021367 .
GeneIDi 13614.
KEGGi mmu:13614.
UCSCi uc007qfl.2. mouse.

Organism-specific databases

CTDi 1906.
MGIi MGI:95283. Edn1.

Phylogenomic databases

eggNOGi NOG46593.
HOGENOMi HOG000231110.
HOVERGENi HBG051441.
InParanoidi P22387.
KOi K16366.
OMAi RCQCASQ.
OrthoDBi EOG7PS1H7.
PhylomeDBi P22387.
TreeFami TF333184.

Enzyme and pathway databases

Reactomei REACT_207651. G alpha (q) signalling events.
REACT_235286. Peptide ligand-binding receptors.

Miscellaneous databases

ChiTaRSi Edn1. mouse.
NextBioi 284272.
PROi P22387.
SOURCEi Search...

Gene expression databases

Bgeei P22387.
CleanExi MM_EDN1.
ExpressionAtlasi P22387. baseline and differential.
Genevestigatori P22387.

Family and domain databases

InterProi IPR020475. Bibrotoxin/Sarafotoxin-D.
IPR019764. Endothelin_toxin_CS.
IPR001928. Endothln-like_toxin.
[Graphical view ]
Pfami PF00322. Endothelin. 1 hit.
[Graphical view ]
PRINTSi PR00365. ENDOTHELIN.
SMARTi SM00272. END. 2 hits.
[Graphical view ]
PROSITEi PS00270. ENDOTHELIN. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse preproendothelin-1 gene. cDNA cloning, sequence analysis and determination of sites of expression during embryonic development."
    Chan T.S., Lin C.X., Chan W.Y., Chung S.S., Chung S.K.
    Eur. J. Biochem. 234:819-826(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X CBA.
    Tissue: Lung.
  2. "Sequence and neuronal expression of mouse endothelin-1 cDNA."
    Kurama M., Ishida N., Matsui M., Saida K., Mitsui Y.
    Biochim. Biophys. Acta 1307:249-253(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Lung.
  3. "Sequence analysis, chromosomal location, and developmental expression of the mouse preproendothelin-1 gene."
    Maemura K., Kurihara H., Kurihara Y., Oda H., Ishikawa T., Copeland N.G., Gilbert D.J., Jenkins N.A., Yazaki Y.
    Genomics 31:177-184(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: BALB/c.
    Tissue: Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N-3.
    Tissue: Mammary gland.
  5. "Targeting gene expression to the vascular wall in transgenic mice using the murine preproendothelin-1 promoter."
    Harats D., Kurihara H., Belloni P., Oakley H., Ziober A., Ackley D., Cain G., Kurihara Y., Lawn R., Sigal E.
    J. Clin. Invest. 95:1335-1344(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
    Strain: BALB/c.
  6. "Endothelin-1 mRNA in glomerular and epithelial cells of kidney."
    Chen M., Todd-Turla K., Wang W.H., Cao X., Smart A., Brosius F.C., Killen P.D., Keiser J.A., Briggs J.P., Schnermann J.
    Am. J. Physiol. 265:F542-F550(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-76.
    Tissue: Kidney.
  7. "A novel peptide, vasoactive intestinal contractor, of a new (endothelin) peptide family. Molecular cloning, expression, and biological activity."
    Saida K., Mitsui Y., Ishida N.
    J. Biol. Chem. 264:14613-14616(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 48-77.
  8. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 155-165, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.

Entry informationi

Entry nameiEDN1_MOUSE
AccessioniPrimary (citable) accession number: P22387
Secondary accession number(s): P70203, P97338
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 134 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.