P22387 (EDN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endothelin-1
Short name=ET-1

Alternative name(s):
Preproendothelin-1
Short name=PPET1

Gene names

Name:

Edn1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Endothelins are endothelium-derived vasoconstrictor peptides.
Subcellular location	Secreted.
Tissue specificity	Highest expression in the adult is in lung. Lower levels found in heart, kidney, brain and intestine. In the embryo, expressed in outer and inner pharyngeal arch surfaces. Also expressed in endothelium of dorsal aorta and arch arteries, and in epithelium of pharyngeal pouches. Ref.3
Developmental stage	Expression in lung is low at 17.5 dpc fetal mice and increases progressively into adulthood. Ref.3
Sequence similarities	Belongs to the endothelin/sarafotoxin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Vasoactive Vasoconstrictor
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	body fluid secretion Inferred from mutant phenotype PubMed 15314687. Source: MGI calcium-mediated signaling Inferred from electronic annotation. Source: Compara cartilage development Inferred from mutant phenotype PubMed 8152482. Source: MGI cell growth Inferred from electronic annotation. Source: Compara cellular response to interleukin-1 Inferred from electronic annotation. Source: Compara cellular response to tumor necrosis factor Inferred from electronic annotation. Source: Compara dorsal/ventral pattern formation Inferred from mutant phenotype PubMed 15110048. Source: MGI elevation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Inferred from direct assay PubMed 2542249. Source: MGI epithelial fluid transport Inferred from electronic annotation. Source: Compara glucose transport Inferred from direct assay PubMed 11546805. Source: MGI heart development Inferred from mutant phenotype PubMed 7615798. Source: MGI histamine secretion Inferred from electronic annotation. Source: Compara in utero embryonic development Inferred from mutant phenotype PubMed 15327783. Source: MGI inositol phosphate-mediated signaling Inferred from electronic annotation. Source: Compara intracellular protein kinase cascade Inferred from electronic annotation. Source: Compara maternal process involved in parturition Inferred from electronic annotation. Source: Compara membrane depolarization Inferred from electronic annotation. Source: Compara middle ear morphogenesis Inferred from mutant phenotype PubMed 8152482. Source: MGI multicellular organismal aging Inferred from electronic annotation. Source: Compara negative regulation of cAMP biosynthetic process Inferred from direct assay PubMed 2542249. Source: MGI negative regulation of cellular protein metabolic process Inferred from electronic annotation. Source: Compara negative regulation of hormone secretion Inferred from electronic annotation. Source: Compara negative regulation of nitric-oxide synthase biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of smooth muscle cell apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara neural crest cell development Inferred from mutant phenotype PubMed 15327783PubMed 9671575. Source: MGI nitric oxide transport Inferred from electronic annotation. Source: Compara patterning of blood vessels Inferred from mutant phenotype PubMed 7615798. Source: MGI peptide hormone secretion Inferred from electronic annotation. Source: Compara phosphatidylinositol 3-kinase cascade Inferred from electronic annotation. Source: Compara phospholipase D-activating G-protein coupled receptor signaling pathway Inferred from direct assay PubMed 8978712. Source: MGI positive regulation of JUN kinase activity Inferred from electronic annotation. Source: Compara positive regulation of cardiac muscle hypertrophy Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from direct assay PubMed 2542249. Source: MGI positive regulation of cell size Inferred from electronic annotation. Source: Compara positive regulation of heart rate Inferred from electronic annotation. Source: Compara positive regulation of hormone secretion Inferred from electronic annotation. Source: Compara positive regulation of mitosis Inferred from electronic annotation. Source: Compara positive regulation of neutrophil chemotaxis Inferred from electronic annotation. Source: Compara positive regulation of odontogenesis Inferred from electronic annotation. Source: Compara positive regulation of prostaglandin-endoperoxide synthase activity Inferred from electronic annotation. Source: Compara positive regulation of receptor biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of renal sodium excretion Inferred from mutant phenotype PubMed 15314687. Source: MGI positive regulation of sarcomere organization Inferred from electronic annotation. Source: Compara positive regulation of smooth muscle cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of smooth muscle contraction Inferred from electronic annotation. Source: Compara positive regulation of urine volume Inferred from mutant phenotype PubMed 15632412. Source: MGI prostaglandin biosynthetic process Inferred from electronic annotation. Source: Compara protein kinase C deactivation Inferred from electronic annotation. Source: Compara protein kinase C-activating G-protein coupled receptor signaling pathway Inferred from direct assay PubMed 2542249. Source: MGI regulation of blood pressure Inferred from mutant phenotype PubMed 10626068PubMed 15314687PubMed 8152482. Source: MGI regulation of pH Inferred from direct assay PubMed 11413164. Source: MGI regulation of sensory perception of pain Inferred from electronic annotation. Source: Compara regulation of systemic arterial blood pressure by endothelin Inferred from electronic annotation. Source: Compara regulation of vasoconstriction Inferred from electronic annotation. Source: InterPro respiratory gaseous exchange Inferred from mutant phenotype PubMed 10626068. Source: MGI response to amino acid stimulus Inferred from electronic annotation. Source: Compara response to hypoxia Inferred from mutant phenotype PubMed 8764295. Source: MGI response to lipopolysaccharide Inferred from electronic annotation. Source: Compara response to nicotine Inferred from electronic annotation. Source: Compara response to testosterone stimulus Inferred from electronic annotation. Source: Compara rhythmic excitation Inferred from mutant phenotype PubMed 8764295. Source: MGI sensory perception of pain Inferred from electronic annotation. Source: Compara superoxide anion generation Inferred from electronic annotation. Source: Compara vein smooth muscle contraction Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara extracellular space Inferred from direct assay PubMed 15314687. Source: MGI
Molecular_function	receptor binding Inferred from direct assay PubMed 2542249PubMed 8680799. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Propeptide	26 – 50	25			PRO_0000008062
	Peptide	53 – 73	21	Endothelin-1		PRO_0000008063
	Propeptide	74 – 202	129			PRO_0000008064
Regions
	Region	110 – 124	15	Endothelin-like
Sites
	Site	73 – 74	2	Cleavage; by KEL By similarity
Amino acid modifications
	Disulfide bond	53 ↔ 67		By similarity
	Disulfide bond	55 ↔ 63		By similarity
Experimental info
	Sequence conflict	75	1	N → K in BAA11109. Ref.3
	Sequence conflict	103	1	A → G in BAA07830. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P22387 [UniParc]. Last modified October 1, 1996. Version 3. Checksum: 3A0C1E1DFA797411	FASTA	202	22,809
	10 20 30 40 50 60 MDYFPVIFSL LFVTFQGAPE TAVLGAELST GAENGVQSPP PSTPWRPRRS KRCSCSSLMD 70 80 90 100 110 120 KECVYFCHLD IIWVNTPERV VPYGLGGSSR SKRSLKDLLP NKATDQAVRC QCAHQKDKKC 130 140 150 160 170 180 WNFCQAGKEL RAQSTMQKSL KDSKKGKPCS KLGKKCIYQQ LVEGRKLRRL EAISNSIKAS 190 200 FRVAKLKAEL YRDQKLTHNR AH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse preproendothelin-1 gene. cDNA cloning, sequence analysis and determination of sites of expression during embryonic development." Chan T.S., Lin C.X., Chan W.Y., Chung S.S., Chung S.K. Eur. J. Biochem. 234:819-826(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6 X CBA. Tissue: Lung.
[2]	"Sequence and neuronal expression of mouse endothelin-1 cDNA." Kurama M., Ishida N., Matsui M., Saida K., Mitsui Y. Biochim. Biophys. Acta 1307:249-253(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Lung.
[3]	"Sequence analysis, chromosomal location, and developmental expression of the mouse preproendothelin-1 gene." Maemura K., Kurihara H., Kurihara Y., Oda H., Ishikawa T., Copeland N.G., Gilbert D.J., Jenkins N.A., Yazaki Y. Genomics 31:177-184(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: BALB/c. Tissue: Liver.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N-3. Tissue: Mammary gland.
[5]	"Targeting gene expression to the vascular wall in transgenic mice using the murine preproendothelin-1 promoter." Harats D., Kurihara H., Belloni P., Oakley H., Ziober A., Ackley D., Cain G., Kurihara Y., Lawn R., Sigal E. J. Clin. Invest. 95:1335-1344(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21. Strain: BALB/c.
[6]	"Endothelin-1 mRNA in glomerular and epithelial cells of kidney." Chen M., Todd-Turla K., Wang W.H., Cao X., Smart A., Brosius F.C., Killen P.D., Keiser J.A., Briggs J.P., Schnermann J. Am. J. Physiol. 265:F542-F550(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-76. Tissue: Kidney.
[7]	"A novel peptide, vasoactive intestinal contractor, of a new (endothelin) peptide family. Molecular cloning, expression, and biological activity." Saida K., Mitsui Y., Ishida N. J. Biol. Chem. 264:14613-14616(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 48-77.
[8]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 155-165, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U35233 mRNA. Translation: AAA97608.1. D43775 mRNA. Translation: BAA07830.1. D70842 Genomic DNA. Translation: BAA11109.1. BC029547 mRNA. Translation: AAH29547.1. U07982 Genomic DNA. Translation: AAA74439.1. Sequence problems. S66650 mRNA. Translation: AAP13983.1. M26497 Genomic DNA. No translation available.
IPI	IPI00127462.
PIR	S63538.
RefSeq	NP_034234.1. NM_010104.3.
UniGene	Mm.14543.
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	P22387.
Proteomic databases
PRIDE	P22387.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000021796; ENSMUSP00000021796; ENSMUSG00000021367.
GeneID	13614.
KEGG	mmu:13614.
Organism-specific databases
CTD	1906.
MGI	MGI:95283. Edn1.
Phylogenomic databases
eggNOG	NOG46593.
HOGENOM	HOG000231110.
HOVERGEN	HBG051441.
InParanoid	P22387.
KO	K16366.
OMA	RCQCASQ.
OrthoDB	EOG4QZ7N8.
Gene expression databases
ArrayExpress	P22387.
Bgee	P22387.
CleanEx	MM_EDN1.
Genevestigator	P22387.
GermOnline	ENSMUSG00000021367. Mus musculus.
Family and domain databases
InterPro	IPR020475. Bibrotoxin/Sarafotoxin-D. IPR019764. Endothelin_toxin_CS. IPR001928. Endothln-like_toxin. [Graphical view]
Pfam	PF00322. Endothelin. 1 hit. [Graphical view]
PRINTS	PR00365. ENDOTHELIN.
SMART	SM00272. END. 2 hits. [Graphical view]
PROSITE	PS00270. ENDOTHELIN. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	EDN1. mouse.
NextBio	284272.
SOURCE	Search...

Entry information

Entry name

EDN1_MOUSE

Accession

Primary (citable) accession number: P22387
Secondary accession number(s): P70203, P97338

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 119 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program