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P22387 (EDN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endothelin-1

Short name=ET-1
Alternative name(s):
Preproendothelin-1
Short name=PPET1
Gene names
Name:Edn1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length202 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Endothelins are endothelium-derived vasoconstrictor peptides.

Subcellular location

Secreted.

Tissue specificity

Highest expression in the adult is in lung. Lower levels found in heart, kidney, brain and intestine. In the embryo, expressed in outer and inner pharyngeal arch surfaces. Also expressed in endothelium of dorsal aorta and arch arteries, and in epithelium of pharyngeal pouches. Ref.3

Developmental stage

Expression in lung is low at 17.5 dpc fetal mice and increases progressively into adulthood. Ref.3

Sequence similarities

Belongs to the endothelin/sarafotoxin family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionVasoactive
Vasoconstrictor
   PTMCleavage on pair of basic residues
Disulfide bond
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Traceable author statement PubMed 11546805. Source: MGI

blood vessel morphogenesis

Inferred from mutant phenotype PubMed 7615798. Source: MGI

body fluid secretion

Inferred from mutant phenotype PubMed 15314687. Source: MGI

calcium-mediated signaling

Inferred from electronic annotation. Source: Ensembl

cartilage development

Inferred from mutant phenotype PubMed 8152482. Source: MGI

cell growth

Inferred from electronic annotation. Source: Ensembl

cellular response to interleukin-1

Inferred from electronic annotation. Source: Ensembl

cellular response to tumor necrosis factor

Inferred from electronic annotation. Source: Ensembl

dorsal/ventral pattern formation

Inferred from mutant phenotype PubMed 15110048. Source: MGI

epithelial fluid transport

Inferred from electronic annotation. Source: Ensembl

glucose transport

Inferred from direct assay PubMed 11546805. Source: MGI

heart development

Inferred from mutant phenotype PubMed 7615798. Source: MGI

histamine secretion

Inferred from electronic annotation. Source: Ensembl

in utero embryonic development

Inferred from mutant phenotype PubMed 15327783. Source: MGI

inositol phosphate-mediated signaling

Inferred from electronic annotation. Source: Ensembl

maternal process involved in parturition

Inferred from electronic annotation. Source: Ensembl

membrane depolarization

Inferred from electronic annotation. Source: Ensembl

middle ear morphogenesis

Inferred from mutant phenotype PubMed 8152482. Source: MGI

multicellular organismal aging

Inferred from electronic annotation. Source: Ensembl

negative regulation of cAMP biosynthetic process

Inferred from direct assay PubMed 2542249. Source: MGI

negative regulation of cellular protein metabolic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of hormone secretion

Inferred from electronic annotation. Source: Ensembl

negative regulation of nitric-oxide synthase biosynthetic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of smooth muscle cell apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

neural crest cell development

Inferred from mutant phenotype PubMed 15327783PubMed 9671575. Source: MGI

nitric oxide transport

Inferred from electronic annotation. Source: Ensembl

patterning of blood vessels

Inferred from mutant phenotype PubMed 7615798. Source: MGI

peptide hormone secretion

Inferred from electronic annotation. Source: Ensembl

phosphatidylinositol 3-kinase signaling

Inferred from electronic annotation. Source: Ensembl

phospholipase D-activating G-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 8978712. Source: MGI

positive regulation of JUN kinase activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of cardiac muscle hypertrophy

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation

Inferred from direct assay PubMed 2542249. Source: MGI

positive regulation of cell size

Inferred from electronic annotation. Source: Ensembl

positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway

Inferred from direct assay PubMed 2542249. Source: MGI

positive regulation of heart rate

Inferred from electronic annotation. Source: Ensembl

positive regulation of hormone secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of mitosis

Inferred from electronic annotation. Source: Ensembl

positive regulation of neutrophil chemotaxis

Inferred from electronic annotation. Source: Ensembl

positive regulation of odontogenesis

Inferred from electronic annotation. Source: Ensembl

positive regulation of prostaglandin-endoperoxide synthase activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of receptor biosynthetic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of renal sodium excretion

Inferred from mutant phenotype PubMed 15314687. Source: MGI

positive regulation of sarcomere organization

Inferred from electronic annotation. Source: Ensembl

positive regulation of smooth muscle cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of smooth muscle contraction

Inferred from electronic annotation. Source: Ensembl

positive regulation of urine volume

Inferred from mutant phenotype PubMed 15632412. Source: MGI

prostaglandin biosynthetic process

Inferred from electronic annotation. Source: Ensembl

protein kinase C deactivation

Inferred from electronic annotation. Source: Ensembl

protein kinase C-activating G-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 2542249. Source: MGI

regulation of blood pressure

Inferred from mutant phenotype PubMed 10626068PubMed 15314687PubMed 8152482. Source: MGI

regulation of pH

Inferred from direct assay PubMed 11413164. Source: MGI

regulation of sensory perception of pain

Inferred from electronic annotation. Source: Ensembl

regulation of systemic arterial blood pressure by endothelin

Inferred from electronic annotation. Source: Ensembl

regulation of vasoconstriction

Inferred from electronic annotation. Source: InterPro

respiratory gaseous exchange

Inferred from mutant phenotype PubMed 10626068. Source: MGI

response to amino acid

Inferred from electronic annotation. Source: Ensembl

response to hypoxia

Inferred from mutant phenotype PubMed 8764295. Source: MGI

response to lipopolysaccharide

Inferred from electronic annotation. Source: Ensembl

response to nicotine

Inferred from electronic annotation. Source: Ensembl

response to testosterone

Inferred from electronic annotation. Source: Ensembl

rhythmic excitation

Inferred from mutant phenotype PubMed 8764295. Source: MGI

sensory perception of pain

Inferred from electronic annotation. Source: Ensembl

skeletal system development

Inferred from mutant phenotype PubMed 8152482. Source: MGI

superoxide anion generation

Inferred from electronic annotation. Source: Ensembl

vein smooth muscle contraction

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: Ensembl

extracellular space

Inferred from direct assay PubMed 15314687. Source: MGI

   Molecular_functionendothelin A receptor binding

Inferred from physical interaction PubMed 9449664. Source: MGI

receptor binding

Inferred from direct assay PubMed 2542249PubMed 8680799. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Propeptide26 – 5025
PRO_0000008062
Peptide53 – 7321Endothelin-1
PRO_0000008063
Propeptide74 – 202129
PRO_0000008064

Regions

Region110 – 12415Endothelin-like

Sites

Site73 – 742Cleavage; by KEL By similarity

Amino acid modifications

Disulfide bond53 ↔ 67 By similarity
Disulfide bond55 ↔ 63 By similarity

Experimental info

Sequence conflict751N → K in BAA11109. Ref.3
Sequence conflict1031A → G in BAA07830. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P22387 [UniParc].

Last modified October 1, 1996. Version 3.
Checksum: 3A0C1E1DFA797411

FASTA20222,809
        10         20         30         40         50         60 
MDYFPVIFSL LFVTFQGAPE TAVLGAELST GAENGVQSPP PSTPWRPRRS KRCSCSSLMD 

        70         80         90        100        110        120 
KECVYFCHLD IIWVNTPERV VPYGLGGSSR SKRSLKDLLP NKATDQAVRC QCAHQKDKKC 

       130        140        150        160        170        180 
WNFCQAGKEL RAQSTMQKSL KDSKKGKPCS KLGKKCIYQQ LVEGRKLRRL EAISNSIKAS 

       190        200 
FRVAKLKAEL YRDQKLTHNR AH 

« Hide

References

« Hide 'large scale' references
[1]"Mouse preproendothelin-1 gene. cDNA cloning, sequence analysis and determination of sites of expression during embryonic development."
Chan T.S., Lin C.X., Chan W.Y., Chung S.S., Chung S.K.
Eur. J. Biochem. 234:819-826(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6 X CBA.
Tissue: Lung.
[2]"Sequence and neuronal expression of mouse endothelin-1 cDNA."
Kurama M., Ishida N., Matsui M., Saida K., Mitsui Y.
Biochim. Biophys. Acta 1307:249-253(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Lung.
[3]"Sequence analysis, chromosomal location, and developmental expression of the mouse preproendothelin-1 gene."
Maemura K., Kurihara H., Kurihara Y., Oda H., Ishikawa T., Copeland N.G., Gilbert D.J., Jenkins N.A., Yazaki Y.
Genomics 31:177-184(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: BALB/c.
Tissue: Liver.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N-3.
Tissue: Mammary gland.
[5]"Targeting gene expression to the vascular wall in transgenic mice using the murine preproendothelin-1 promoter."
Harats D., Kurihara H., Belloni P., Oakley H., Ziober A., Ackley D., Cain G., Kurihara Y., Lawn R., Sigal E.
J. Clin. Invest. 95:1335-1344(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
Strain: BALB/c.
[6]"Endothelin-1 mRNA in glomerular and epithelial cells of kidney."
Chen M., Todd-Turla K., Wang W.H., Cao X., Smart A., Brosius F.C., Killen P.D., Keiser J.A., Briggs J.P., Schnermann J.
Am. J. Physiol. 265:F542-F550(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-76.
Tissue: Kidney.
[7]"A novel peptide, vasoactive intestinal contractor, of a new (endothelin) peptide family. Molecular cloning, expression, and biological activity."
Saida K., Mitsui Y., Ishida N.
J. Biol. Chem. 264:14613-14616(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 48-77.
[8]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 155-165, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U35233 mRNA. Translation: AAA97608.1.
D43775 mRNA. Translation: BAA07830.1.
D70842 Genomic DNA. Translation: BAA11109.1.
BC029547 mRNA. Translation: AAH29547.1.
U07982 Genomic DNA. Translation: AAA74439.1. Sequence problems.
S66650 mRNA. Translation: AAP13983.1.
M26497 Genomic DNA. No translation available.
PIRS63538.
RefSeqNP_034234.1. NM_010104.3.
UniGeneMm.14543.

3D structure databases

ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP22387.

Proteomic databases

PRIDEP22387.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021796; ENSMUSP00000021796; ENSMUSG00000021367.
GeneID13614.
KEGGmmu:13614.
UCSCuc007qfl.2. mouse.

Organism-specific databases

CTD1906.
MGIMGI:95283. Edn1.

Phylogenomic databases

eggNOGNOG46593.
HOGENOMHOG000231110.
HOVERGENHBG051441.
InParanoidP22387.
KOK16366.
OMARCQCASQ.
OrthoDBEOG7PS1H7.
PhylomeDBP22387.
TreeFamTF333184.

Gene expression databases

ArrayExpressP22387.
BgeeP22387.
CleanExMM_EDN1.
GenevestigatorP22387.

Family and domain databases

InterProIPR020475. Bibrotoxin/Sarafotoxin-D.
IPR019764. Endothelin_toxin_CS.
IPR001928. Endothln-like_toxin.
[Graphical view]
PfamPF00322. Endothelin. 1 hit.
[Graphical view]
PRINTSPR00365. ENDOTHELIN.
SMARTSM00272. END. 2 hits.
[Graphical view]
PROSITEPS00270. ENDOTHELIN. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSEDN1. mouse.
NextBio284272.
PROP22387.
SOURCESearch...

Entry information

Entry nameEDN1_MOUSE
AccessionPrimary (citable) accession number: P22387
Secondary accession number(s): P70203, P97338
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot