Reviewed, UniProtKB/Swiss-Prot P22366 (MYD88_MOUSE)

Names and origin

Protein names

Recommended name:
    Myeloid differentiation primary response protein MyD88

Gene names

Name:

Myd88

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. May be involved in myeloid differentiation. Ref.5
Subunit structure	Homodimer. Also forms heterodimers with TIRAP. Binds to TLR2, TLR4, IRAK1 and IRAK2 via their respective TIR domains. Interacts with IL1RL1 By similarity.
Subcellular location	Cytoplasm Probable.
Tissue specificity	Ubiquitous. Ref.1
Induction	By interleukin-6.
Sequence similarities	Contains 1 death domain. Contains 1 TIR domain.

Ontologies

Keywords
Biological process	Immune response Inflammatory response
Cellular component	Cytoplasm
Gene Ontology (GO)
Biological process	MyD88-dependent toll-like receptor signaling pathway Inferred from mutant phenotype. Source: MGI immunoglobulin mediated immune response Inferred from mutant phenotype. Source: MGI inflammatory response Inferred from electronic annotation. Source: UniProtKB-KW innate immune response Inferred from electronic annotation. Source: InterPro lipopolysaccharide-mediated signaling pathway Inferred from mutant phenotype. Source: MGI positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from genetic interaction. Source: MGI positive regulation of JNK cascade Inferred from mutant phenotype. Source: MGI positive regulation of NF-kappaB transcription factor activity Inferred from genetic interaction. Source: MGI positive regulation of chemokine biosynthetic process Inferred from genetic interaction. Source: MGI positive regulation of tumor necrosis factor production Inferred from mutant phenotype. Source: MGI response to lipopolysaccharide Inferred from mutant phenotype. Source: MGI response to molecule of fungal origin Inferred from mutant phenotype. Source: MGI response to virus Inferred from mutant phenotype. Source: MGI transmembrane receptor protein serine/threonine kinase signaling pathway Traceable author statement. Source: MGI type I interferon biosynthetic process Inferred from mutant phenotype. Source: MGI
Cellular component	cytoplasm Traceable author statement. Source: MGI intrinsic to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	transmembrane receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Il1rl1	P14719	1	EBI-525108,EBI-525078
Irak1	Q62406	1	EBI-525108,EBI-448533

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Myeloid differentiation primary response protein MyD88		PRO_0000096667
Regions
	Domain	54 – 109	56	Death
	Domain	159 – 296	138	TIR
Experimental info
	Mutagenesis	56	1	F → N: Prevents dimerization of death domains and activation of JNK and NF-kappa-B. Ref.5
	Sequence conflict	51	1	A → P Ref.1
	Sequence conflict	51	1	A → P Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P22366-1 [UniParc]. Last modified March 27, 2002. Version 3. Checksum: 393A75C3723FFE69	FASTA	296	33,753
	10 20 30 40 50 60 MSAGDPRVGS GSLDSFMFSI PLVALNVGVR RRLSLFLNPR TPVAADWTLL AEEMGFEYLE 70 80 90 100 110 120 IRELETRPDP TRSLLDAWQG RSGASVGRLL ELLALLDRED ILKELKSRIE EDCQKYLGKQ 130 140 150 160 170 180 QNQESEKPLQ VARVESSVPQ TKELGGITTL DDPLGQTPEL FDAFICYCPN DIEFVQEMIR 190 200 210 220 230 240 QLEQTDYRLK LCVSDRDVLP GTCVWSIASE LIEKRCRRMV VVVSDDYLQS KECDFQTKFA 250 260 270 280 290 LSLSPGVQQK RLIPIKYKAM KKDFPSILRF ITICDYTNPC TKSWFWTRLA KALSLP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cloning and characterization of human MyD88: a member of an IL-1 receptor related family." Bonnert T.P., Garka K.E., Parnet P., Sonoda G., Testa J.R., Sims J.E. FEBS Lett. 402:81-84(1997) [PubMed: 9013863] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"Genetic structure and chromosomal mapping of MyD88." Hardiman G., Jenkins N.A., Copeland N.G., Gilbert D.J., Garcia D.K., Naylor S.L., Kastelein R.A., Bazan J.F. Genomics 45:332-339(1997) [PubMed: 9344657] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Myeloid leukemia cell.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Mammary gland and Olfactory epithelium.
[4]	"Nucleotide sequence and expression of a cDNA encoding MyD88, a novel myeloid differentiation primary response gene induced by IL6." Lord K.A., Hoffman-Liebermann B., Liebermann D.A. Oncogene 5:1095-1097(1990) [PubMed: 2374694] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-296.
[5]	"MyD88, an adapter protein involved in interleukin-1 signaling." Burns K., Martinon F., Esslinger C., Pahl H., Schneider P., Bodmer J.-L., Di Marco F., French L., Tschopp J. J. Biol. Chem. 273:12203-12209(1998) [PubMed: 9575168] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF PHE-56. Tissue: Bone marrow.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U89023 Genomic DNA. Translation: AAB83958.1. U84409 mRNA. Translation: AAC53013.1. BC005591 mRNA. Translation: AAH05591.1. BC058787 mRNA. Translation: AAH58787.1. X51397 mRNA. Translation: CAA35762.1.
IPI	IPI00127375.
PIR	S11226.
RefSeq	NP_034981.1.
UniGene	Mm.213003
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P22366. 6 interactions.
PTM databases
PhosphoSite	P22366.
Proteomic databases
PRIDE	P22366.
Genome annotation databases
Ensembl	ENSMUSG00000032508. Mus musculus. [Contig view]
GeneID	17874.
KEGG	mmu:17874.
Organism-specific databases
MGI	MGI:108005. Myd88.
Phylogenomic databases
HOGENOM	P22366.
HOVERGEN	P22366.
OMA	P22366. GFEYLEI.
Gene expression databases
ArrayExpress	P22366.
Bgee	P22366.
CleanEx	MM_MYD88.
GermOnline	ENSMUSG00000032508. Mus musculus.
Family and domain databases
InterPro	IPR000488. Death. IPR011029. DEATH-like. IPR017281. Myelin_different_resp_MyD88. IPR000157. Toll-Interleukin_rcpt. [Graphical view]
Gene3D	G3DSA:1.10.533.10. DEATH_like. 1 hit.
Pfam	PF00531. Death. 1 hit. PF01582. TIR. 1 hit. [Graphical view]
PIRSF	PIRSF037756. MyD88. 1 hit.
SMART	SM00005. DEATH. 1 hit. SM00255. TIR. 1 hit. [Graphical view]
PROSITE	PS50017. DEATH_DOMAIN. 1 hit. PS50104. TIR. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	292657.
SOURCE	Search...

Entry information

Entry name

MYD88_MOUSE

Accession

Primary (citable) accession number: P22366
Secondary accession number(s): O35916

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	March 27, 2002
Last modified:	June 16, 2009
This is version 88 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)