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P22360

- KPYK_EMENI

UniProt

P22360 - KPYK_EMENI

Protein

Pyruvate kinase

Gene

pkiA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (01 May 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.
    Potassium.

    Enzyme regulationi

    Regulated by phosphoenolpyruvate substrate and allosteric effectors such as fructose 1,6 diphosphate and magnesium.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei63 – 631SubstrateBy similarity
    Metal bindingi65 – 651PotassiumBy similarity
    Metal bindingi67 – 671PotassiumBy similarity
    Metal bindingi98 – 981PotassiumBy similarity
    Metal bindingi99 – 991Potassium; via carbonyl oxygenBy similarity
    Sitei254 – 2541Transition state stabilizerBy similarity
    Metal bindingi256 – 2561MagnesiumSequence Analysis
    Binding sitei279 – 2791Substrate; via amide nitrogenBy similarity
    Metal bindingi280 – 2801MagnesiumBy similarity
    Binding sitei280 – 2801Substrate; via amide nitrogenBy similarity
    Binding sitei312 – 3121SubstrateBy similarity
    Binding sitei351 – 3511ADPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: ASPGD

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:pkiA
    Synonyms:pki
    ORF Names:AN5210
    OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
    Taxonomic identifieri227321 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000000560: Chromosome V

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: ASPGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 526526Pyruvate kinasePRO_0000112113Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei36 – 361PhosphoserineSequence Analysis

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP22360.

    Interactioni

    Subunit structurei

    Homotetramer.

    Protein-protein interaction databases

    STRINGi162425.CADANIAP00003208.

    Structurei

    3D structure databases

    ProteinModelPortaliP22360.
    SMRiP22360. Positions 31-515.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    HOGENOMiHOG000021559.
    KOiK00873.
    OMAiCIVENAG.
    OrthoDBiEOG7M6DJC.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P22360-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAASSSLDHL SNRMKLEWHS KLNTEMVPAK NFRRTSIICT IGPKTNSVEK    50
    INALRRAGLN VVRMNFSHGS YEYHQSVIDH AREAEKQQAG RPVAIALDTK 100
    GPEIRTGNTV GDKDIPIKAG HEMNISTDEQ YATASDDQNM YVDYKNITKV 150
    ISAGKLIYVD DGILSFEVLE VVDDKTLRVR CLNNGNISSR KGVNLPGTDV 200
    DLPALSEKDI SDLKFGVKNK VDMVFASFIR RGSDIRHIRE VLGEEGREIQ 250
    IIAKIENQQG VNNFDEILEE TDGVMVARGD LGIEIPAPKV FIAQKMMIAK 300
    CNIKGKPVIC ATQMLESMTY NPRPTRAEVS DVANAVLDGA DCVMLSGETA 350
    KGNYPCEAVT MMSETCLLAE VAIPHFNVFD ELRNLAPRPT DTVESIAMAA 400
    VSASLELNAG AIVVLTTSGN TARMISKYRP VCPIIMVSRN PAATRYSHLY 450
    RGVWPFYFPE KKPDFNVKIW QEDVDRRLKW GINHGLKLGI INKGDNIVCV 500
    QGWRGGMGHT NTVRVVPAEE NLGLSE 526
    Length:526
    Mass (Da):58,133
    Last modified:May 1, 2007 - v2
    Checksum:iBCD3A6F35F5D28B2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti88 – 881Q → A in AAA33320. (PubMed:3072099)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36918 Genomic DNA. Translation: AAA33320.1.
    AACD01000089 Genomic DNA. Translation: EAA62391.1.
    BN001305 Genomic DNA. Translation: CBF81089.1.
    PIRiS27364.
    RefSeqiXP_662814.1. XM_657722.1.

    Genome annotation databases

    EnsemblFungiiCADANIAT00003208; CADANIAP00003208; CADANIAG00003208.
    GeneIDi2871501.
    KEGGiani:AN5210.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36918 Genomic DNA. Translation: AAA33320.1 .
    AACD01000089 Genomic DNA. Translation: EAA62391.1 .
    BN001305 Genomic DNA. Translation: CBF81089.1 .
    PIRi S27364.
    RefSeqi XP_662814.1. XM_657722.1.

    3D structure databases

    ProteinModelPortali P22360.
    SMRi P22360. Positions 31-515.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 162425.CADANIAP00003208.

    Proteomic databases

    PRIDEi P22360.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADANIAT00003208 ; CADANIAP00003208 ; CADANIAG00003208 .
    GeneIDi 2871501.
    KEGGi ani:AN5210.2.

    Phylogenomic databases

    eggNOGi COG0469.
    HOGENOMi HOG000021559.
    KOi K00873.
    OMAi CIVENAG.
    OrthoDBi EOG7M6DJC.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of the Aspergillus nidulans pyruvate kinase gene."
      de Graaff L., Visser J.
      Curr. Genet. 14:553-560(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: WG096.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
    3. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
      Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
      , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
      Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

    Entry informationi

    Entry nameiKPYK_EMENI
    AccessioniPrimary (citable) accession number: P22360
    Secondary accession number(s): C8VF86, Q5B2M0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3