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Protein

Pulmonary surfactant-associated protein B

Gene

Sftpb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

GO - Molecular functioni

GO - Biological processi

  • cellular response to mechanical stimulus Source: RGD
  • cellular response to nitric oxide Source: RGD
  • circadian rhythm Source: RGD
  • organ morphogenesis Source: GO_Central
  • regulation of liquid surface tension Source: RGD
  • respiratory gaseous exchange Source: UniProtKB-KW
  • response to epidermal growth factor Source: RGD
  • response to glucocorticoid Source: RGD
  • response to glucose Source: RGD
  • response to growth factor Source: RGD
  • response to hyperoxia Source: RGD
  • response to hypoxia Source: RGD
  • response to interleukin-6 Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to vitamin A Source: RGD
  • sphingolipid metabolic process Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Gaseous exchange

Names & Taxonomyi

Protein namesi
Recommended name:
Pulmonary surfactant-associated protein B
Short name:
SP-B
Alternative name(s):
Pulmonary surfactant-associated proteolipid SPL(Phe)
Gene namesi
Name:Sftpb
Synonyms:Sftp3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621700. Sftpb.

Subcellular locationi

GO - Cellular componenti

  • alveolar lamellar body Source: RGD
  • extracellular space Source: RGD
  • lysosome Source: InterPro
  • multivesicular body Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted, Surface film

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Propeptidei25 – 190166PRO_0000031656Add
BLAST
Chaini191 – 26979Pulmonary surfactant-associated protein BPRO_0000031657Add
BLAST
Propeptidei270 – 376107PRO_0000031658Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi67 ↔ 141PROSITE-ProRule annotation
Disulfide bondi70 ↔ 135PROSITE-ProRule annotation
Disulfide bondi98 ↔ 110PROSITE-ProRule annotation
Disulfide bondi198 ↔ 267PROSITE-ProRule annotation
Disulfide bondi201 ↔ 261PROSITE-ProRule annotation
Disulfide bondi225 ↔ 236PROSITE-ProRule annotation
Disulfide bondi238 – 238InterchainPROSITE-ProRule annotation
Disulfide bondi294 ↔ 361PROSITE-ProRule annotation
Disulfide bondi297 ↔ 355PROSITE-ProRule annotation
Glycosylationi306 – 3061N-linked (GlcNAc...)PROSITE-ProRule annotation
Disulfide bondi320 ↔ 330PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP22355.
PRIDEiP22355.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014505.

Structurei

3D structure databases

ProteinModelPortaliP22355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 6339Saposin A-typePROSITE-ProRule annotationAdd
BLAST
Domaini63 – 14583Saposin B-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini194 – 27178Saposin B-type 2PROSITE-ProRule annotationAdd
BLAST
Domaini290 – 36576Saposin B-type 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 saposin A-type domain.PROSITE-ProRule annotation
Contains 3 saposin B-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1340. Eukaryota.
ENOG410XSI5. LUCA.
HOGENOMiHOG000115745.
HOVERGENiHBG006905.
InParanoidiP22355.
PhylomeDBiP22355.

Family and domain databases

Gene3Di1.10.225.10. 3 hits.
InterProiIPR003119. SAP_A.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR008373. Saposin.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
[Graphical view]
PfamiPF02199. SapA. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 2 hits.
[Graphical view]
PRINTSiPR01797. SAPOSIN.
SMARTiSM00162. SAPA. 1 hit.
SM00741. SapB. 3 hits.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 3 hits.
PROSITEiPS51110. SAP_A. 1 hit.
PS50015. SAP_B. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22355-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKLHLQWLL LLPTLCSLGA ATESASSPDC AQGPKFWCQS LEQAIQCRAL
60 70 80 90 100
GHCLQEVWGH AGANDLCQEC EDIVHLLTKM TKEDAFQDTI RKFLEQECDI
110 120 130 140 150
LPLKLLVPRC RQVLDVYLPL VIDYFQGQIK PKAICSHVGL CPLGQTKPEQ
160 170 180 190 200
KPEMLDAIPN PLLNKLVLPA LPGAFLARPG PHTQDLSEQQ LPIPLPFCWL
210 220 230 240 250
CRTLIKRVQA VIPKGVLAVA VSQVCHVVPL VVGGICQCLA ERYTVLLLDA
260 270 280 290 300
LLGRVVPQLV CGLVLRCSTA DAIGPALPAL EPLIEKWPLQ DTECHFCKSV
310 320 330 340 350
INQAWNTSEQ AMPQAMHQAC LRFWLDRQKC EQFVEQHMPQ LLALVPRSQD
360 370
AHTSCQALGV CEAPASPLQC FQTPHL
Length:376
Mass (Da):41,590
Last modified:August 1, 1991 - v1
Checksum:iF329DC62E733FB4C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14778 mRNA. Translation: CAA32885.1.
PIRiS02766.
RefSeqiNP_620197.1. NM_138842.1.
UniGeneiRn.1952.

Genome annotation databases

GeneIDi192155.
KEGGirno:192155.
UCSCiRGD:621700. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14778 mRNA. Translation: CAA32885.1.
PIRiS02766.
RefSeqiNP_620197.1. NM_138842.1.
UniGeneiRn.1952.

3D structure databases

ProteinModelPortaliP22355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014505.

Proteomic databases

PaxDbiP22355.
PRIDEiP22355.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi192155.
KEGGirno:192155.
UCSCiRGD:621700. rat.

Organism-specific databases

CTDi6439.
RGDi621700. Sftpb.

Phylogenomic databases

eggNOGiKOG1340. Eukaryota.
ENOG410XSI5. LUCA.
HOGENOMiHOG000115745.
HOVERGENiHBG006905.
InParanoidiP22355.
PhylomeDBiP22355.

Miscellaneous databases

PROiP22355.

Family and domain databases

Gene3Di1.10.225.10. 3 hits.
InterProiIPR003119. SAP_A.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR008373. Saposin.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
[Graphical view]
PfamiPF02199. SapA. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 2 hits.
[Graphical view]
PRINTSiPR01797. SAPOSIN.
SMARTiSM00162. SAPA. 1 hit.
SM00741. SapB. 3 hits.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 3 hits.
PROSITEiPS51110. SAP_A. 1 hit.
PS50015. SAP_B. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA and deduced amino acid sequence for the rat hydrophobic pulmonary surfactant-associated protein, SP-B."
    Emrie P.A., Shannon J.M., Mason R.J., Fisher J.H.
    Biochim. Biophys. Acta 994:215-221(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiPSPB_RAT
AccessioniPrimary (citable) accession number: P22355
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: July 6, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.