ID   RM20_YEAST              Reviewed;         195 AA.
AC   P22354; D6VXE5;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 2.
DT   27-MAR-2024, entry version 174.
DE   RecName: Full=Large ribosomal subunit protein mL58 {ECO:0000303|PubMed:24675956};
DE   AltName: Full=54S ribosomal protein L20, mitochondrial;
DE   AltName: Full=YmL20;
DE   Flags: Precursor;
GN   Name=MRPL20; OrderedLocusNames=YKR085C; ORFNames=YKR405;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 19-42.
RX   PubMed=2183197; DOI=10.1093/nar/18.6.1521;
RA   Kitakawa M., Grohmann L., Graack H.-R., Isono K.;
RT   "Cloning and characterization of nuclear genes for two mitochondrial
RT   ribosomal proteins in Saccharomyces cerevisiae.";
RL   Nucleic Acids Res. 18:1521-1529(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8203164; DOI=10.1002/yea.320100210;
RA   Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G.,
RA   Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F.;
RT   "The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae
RT   chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open
RT   reading frames.";
RL   Yeast 10:231-245(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8196765; DOI=10.1038/369371a0;
RA   Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA   Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA   Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA   Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA   Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA   Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA   Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA   Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA   Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA   Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA   Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA   Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA   Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA   Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA   Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA   Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA   Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA   Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA   Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA   van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA   von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA   Becker I., Mewes H.-W.;
RT   "Complete DNA sequence of yeast chromosome XI.";
RL   Nature 369:371-378(1994).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   PROTEIN SEQUENCE OF 111-123, AND SUBUNIT.
RC   STRAIN=07173;
RX   PubMed=2060626; DOI=10.1016/0014-5793(91)80759-v;
RA   Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S.,
RA   Kitakawa M.;
RT   "Extended N-terminal sequencing of proteins of the large ribosomal subunit
RT   from yeast mitochondria.";
RL   FEBS Lett. 284:51-56(1991).
RN   [6]
RP   TRANSPORT INTO MITOCHONDRIA.
RX   PubMed=8513807; DOI=10.1111/j.1432-1033.1993.tb17956.x;
RA   Matsushita Y., Isono K.;
RT   "Mitochondrial transport of mitoribosomal proteins, YmL8 and YmL20, in
RT   Saccharomyces cerevisiae.";
RL   Eur. J. Biochem. 214:577-585(1993).
RN   [7]
RP   IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=12392552; DOI=10.1046/j.1432-1033.2002.03226.x;
RA   Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.;
RT   "Tag-mediated isolation of yeast mitochondrial ribosome and mass
RT   spectrometric identification of its new components.";
RL   Eur. J. Biochem. 269:5203-5214(2002).
RN   [8]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [9]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [10]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 76625 / YPH499;
RX   PubMed=14576278; DOI=10.1073/pnas.2135385100;
RA   Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E.,
RA   Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P.,
RA   Pfanner N., Meisinger C.;
RT   "The proteome of Saccharomyces cerevisiae mitochondria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003).
RN   [11]
RP   SUBCELLULAR LOCATION.
RX   PubMed=25609543; DOI=10.1038/ncomms7019;
RA   Pfeffer S., Woellhaf M.W., Herrmann J.M., Forster F.;
RT   "Organization of the mitochondrial translation machinery studied in situ by
RT   cryoelectron tomography.";
RL   Nat. Commun. 6:6019-6019(2015).
RN   [12]
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.20 ANGSTROMS), AND SUBUNIT.
RX   PubMed=24675956; DOI=10.1126/science.1249410;
RA   Amunts A., Brown A., Bai X.C., Llacer J.L., Hussain T., Emsley P., Long F.,
RA   Murshudov G., Scheres S.H., Ramakrishnan V.;
RT   "Structure of the yeast mitochondrial large ribosomal subunit.";
RL   Science 343:1485-1489(2014).
CC   -!- FUNCTION: Component of the mitochondrial ribosome (mitoribosome), a
CC       dedicated translation machinery responsible for the synthesis of
CC       mitochondrial genome-encoded proteins, including at least some of the
CC       essential transmembrane subunits of the mitochondrial respiratory
CC       chain. The mitoribosomes are attached to the mitochondrial inner
CC       membrane and translation products are cotranslationally integrated into
CC       the membrane. {ECO:0000305|PubMed:24675956,
CC       ECO:0000305|PubMed:25609543}.
CC   -!- SUBUNIT: Component of the mitochondrial large ribosomal subunit (mt-
CC       LSU). Mature yeast 74S mitochondrial ribosomes consist of a small (37S)
CC       and a large (54S) subunit. The 37S small subunit contains a 15S
CC       ribosomal RNA (15S mt-rRNA) and 34 different proteins. The 54S large
CC       subunit contains a 21S rRNA (21S mt-rRNA) and 46 different proteins.
CC       {ECO:0000269|PubMed:12392552, ECO:0000269|PubMed:2060626,
CC       ECO:0000269|PubMed:24675956}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14562095,
CC       ECO:0000269|PubMed:14576278}. Note=Mitoribosomes are tethered to the
CC       mitochondrial inner membrane and spatially aligned with the membrane
CC       insertion machinery through two distinct membrane contact sites, formed
CC       by the 21S rRNA expansion segment 96-ES1 and the inner membrane protein
CC       MBA1. {ECO:0000269|PubMed:25609543}.
CC   -!- MISCELLANEOUS: Present with 350 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the mitochondrion-specific ribosomal protein
CC       mL58 family. {ECO:0000305}.
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DR   EMBL; X53840; CAA37833.1; -; Genomic_DNA.
DR   EMBL; Z27116; CAA81636.1; -; Genomic_DNA.
DR   EMBL; Z28310; CAA82164.1; -; Genomic_DNA.
DR   EMBL; BK006944; DAA09235.1; -; Genomic_DNA.
DR   PIR; S38163; S38163.
DR   RefSeq; NP_013011.1; NM_001179875.1.
DR   PDB; 3J6B; EM; 3.20 A; a=1-195.
DR   PDB; 5MRC; EM; 3.25 A; a=19-195.
DR   PDB; 5MRE; EM; 3.75 A; a=19-195.
DR   PDB; 5MRF; EM; 4.97 A; a=19-195.
DR   PDBsum; 3J6B; -.
DR   PDBsum; 5MRC; -.
DR   PDBsum; 5MRE; -.
DR   PDBsum; 5MRF; -.
DR   AlphaFoldDB; P22354; -.
DR   EMDB; EMD-3551; -.
DR   EMDB; EMD-3552; -.
DR   EMDB; EMD-3553; -.
DR   SMR; P22354; -.
DR   BioGRID; 34216; 159.
DR   ComplexPortal; CPX-1602; 54S mitochondrial large ribosomal subunit.
DR   DIP; DIP-6793N; -.
DR   IntAct; P22354; 10.
DR   MINT; P22354; -.
DR   STRING; 4932.YKR085C; -.
DR   GlyGen; P22354; 3 sites, 1 O-linked glycan (3 sites).
DR   MaxQB; P22354; -.
DR   PaxDb; 4932-YKR085C; -.
DR   PeptideAtlas; P22354; -.
DR   EnsemblFungi; YKR085C_mRNA; YKR085C; YKR085C.
DR   GeneID; 853960; -.
DR   KEGG; sce:YKR085C; -.
DR   AGR; SGD:S000001793; -.
DR   SGD; S000001793; MRPL20.
DR   VEuPathDB; FungiDB:YKR085C; -.
DR   eggNOG; ENOG502S0A4; Eukaryota.
DR   HOGENOM; CLU_089054_1_0_1; -.
DR   InParanoid; P22354; -.
DR   OMA; PEKYTRK; -.
DR   OrthoDB; 2721959at2759; -.
DR   BioCyc; YEAST:G3O-32048-MONOMER; -.
DR   BioGRID-ORCS; 853960; 8 hits in 10 CRISPR screens.
DR   PRO; PR:P22354; -.
DR   Proteomes; UP000002311; Chromosome XI.
DR   RNAct; P22354; Protein.
DR   GO; GO:0005743; C:mitochondrial inner membrane; NAS:ComplexPortal.
DR   GO; GO:0005762; C:mitochondrial large ribosomal subunit; IDA:SGD.
DR   GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR   GO; GO:0003735; F:structural constituent of ribosome; IDA:SGD.
DR   GO; GO:0032543; P:mitochondrial translation; NAS:ComplexPortal.
DR   InterPro; IPR024388; Ribosomal_mL58.
DR   PANTHER; PTHR28266; 54S RIBOSOMAL PROTEIN L20, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR28266:SF1; 54S RIBOSOMAL PROTEIN L20, MITOCHONDRIAL; 1.
DR   Pfam; PF12824; MRP-L20; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Mitochondrion; Reference proteome;
KW   Ribonucleoprotein; Ribosomal protein; Transit peptide.
FT   TRANSIT         1..18
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000269|PubMed:2183197"
FT   CHAIN           19..195
FT                   /note="Large ribosomal subunit protein mL58"
FT                   /id="PRO_0000030573"
FT   CONFLICT        187
FT                   /note="R -> H (in Ref. 1; CAA37833)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   195 AA;  22392 MW;  3165663C8C19EC60 CRC64;
     MIGRGVCCRS FHTAGSAWKQ FGFPKTQVTT IYNKTKSASN YKGYLKHRDA PGMYYQPSES
     IATGSVNSET IPRSFMAASD PRRGLDMPVQ STKAKQCPNV LVGKSTVNGK TYHLGPQEID
     EIRKLRLDNP QKYTRKFLAA KYGISPLFVS MVSKPSEQHV QIMESRLQEI QSRWKEKRRI
     AREDRKRRKL LWYQA
//