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P22354 (RM20_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
54S ribosomal protein L20, mitochondrial
Alternative name(s):
YmL20
Gene names
Name:MRPL20
Ordered Locus Names:YKR085C
ORF Names:YKR405
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length195 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.5 Ref.7

Subcellular location

Mitochondrion Ref.8 Ref.10.

Miscellaneous

Present with 350 molecules/cell in log phase SD medium.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1818Mitochondrion Ref.1
Chain19 – 19517754S ribosomal protein L20, mitochondrial
PRO_0000030573

Regions

Compositional bias113 – 12917Arg/Lys-rich (basic)

Experimental info

Sequence conflict1871R → H in CAA37833. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P22354 [UniParc].

Last modified June 1, 1994. Version 2.
Checksum: 3165663C8C19EC60

FASTA19522,392
        10         20         30         40         50         60 
MIGRGVCCRS FHTAGSAWKQ FGFPKTQVTT IYNKTKSASN YKGYLKHRDA PGMYYQPSES 

        70         80         90        100        110        120 
IATGSVNSET IPRSFMAASD PRRGLDMPVQ STKAKQCPNV LVGKSTVNGK TYHLGPQEID 

       130        140        150        160        170        180 
EIRKLRLDNP QKYTRKFLAA KYGISPLFVS MVSKPSEQHV QIMESRLQEI QSRWKEKRRI 

       190 
AREDRKRRKL LWYQA 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of nuclear genes for two mitochondrial ribosomal proteins in Saccharomyces cerevisiae."
Kitakawa M., Grohmann L., Graack H.-R., Isono K.
Nucleic Acids Res. 18:1521-1529(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 19-42.
[2]"The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames."
Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G., Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F.
Yeast 10:231-245(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria."
Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S., Kitakawa M.
FEBS Lett. 284:51-56(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 111-123, SUBUNIT.
Strain: 07173.
[6]"Mitochondrial transport of mitoribosomal proteins, YmL8 and YmL20, in Saccharomyces cerevisiae."
Matsushita Y., Isono K.
Eur. J. Biochem. 214:577-585(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: TRANSPORT INTO MITOCHONDRIA.
[7]"Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components."
Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.
Eur. J. Biochem. 269:5203-5214(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[8]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X53840 Genomic DNA. Translation: CAA37833.1.
Z27116 Genomic DNA. Translation: CAA81636.1.
Z28310 Genomic DNA. Translation: CAA82164.1.
BK006944 Genomic DNA. Translation: DAA09235.1.
PIRS38163.
RefSeqNP_013011.1. NM_001179875.1.

3D structure databases

ProteinModelPortalP22354.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34216. 29 interactions.
DIPDIP-6793N.
IntActP22354. 9 interactions.
MINTMINT-667597.
STRING4932.YKR085C.

Proteomic databases

PaxDbP22354.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKR085C; YKR085C; YKR085C.
GeneID853960.
KEGGsce:YKR085C.

Organism-specific databases

CYGDYKR085c.
SGDS000001793. MRPL20.

Phylogenomic databases

eggNOGNOG40516.
HOGENOMHOG000001097.
OMASASNYKG.
OrthoDBEOG769ZXC.

Enzyme and pathway databases

BioCycYEAST:G3O-32048-MONOMER.

Gene expression databases

GenevestigatorP22354.

Family and domain databases

InterProIPR024388. Ribosomal_L20_mt.
[Graphical view]
PfamPF12824. MRP-L20. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio975383.

Entry information

Entry nameRM20_YEAST
AccessionPrimary (citable) accession number: P22354
Secondary accession number(s): D6VXE5
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD