P22354 (RM20_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
54S ribosomal protein L20, mitochondrial

Alternative name(s):
YmL20

Gene names

Name:	MRPL20
Ordered Locus Names:	YKR085C
ORF Names:	YKR405

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.5 Ref.7
Subcellular location	Mitochondrion Ref.8 Ref.10.
Miscellaneous	Present with 350 molecules/cell in log phase SD medium.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial translation Inferred by curator Ref.7. Source: SGD
Cellular_component	mitochondrial large ribosomal subunit Inferred from physical interaction Ref.7. Source: SGD
Molecular_function	structural constituent of ribosome Inferred from physical interaction Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 18	18	Mitochondrion Ref.1
	Chain	19 – 195	177	54S ribosomal protein L20, mitochondrial		PRO_0000030573
Regions
	Compositional bias	113 – 129	17	Arg/Lys-rich (basic)
Experimental info
	Sequence conflict	187	1	R → H in CAA37833. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P22354 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: 3165663C8C19EC60	FASTA	195	22,392
	10 20 30 40 50 60 MIGRGVCCRS FHTAGSAWKQ FGFPKTQVTT IYNKTKSASN YKGYLKHRDA PGMYYQPSES 70 80 90 100 110 120 IATGSVNSET IPRSFMAASD PRRGLDMPVQ STKAKQCPNV LVGKSTVNGK TYHLGPQEID 130 140 150 160 170 180 EIRKLRLDNP QKYTRKFLAA KYGISPLFVS MVSKPSEQHV QIMESRLQEI QSRWKEKRRI 190 AREDRKRRKL LWYQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of nuclear genes for two mitochondrial ribosomal proteins in Saccharomyces cerevisiae." Kitakawa M., Grohmann L., Graack H.-R., Isono K. Nucleic Acids Res. 18:1521-1529(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 19-42.
[2]	"The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames." Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G., Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F. Yeast 10:231-245(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria." Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S., Kitakawa M. FEBS Lett. 284:51-56(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 111-123, SUBUNIT. Strain: 07173.
[6]	"Mitochondrial transport of mitoribosomal proteins, YmL8 and YmL20, in Saccharomyces cerevisiae." Matsushita Y., Isono K. Eur. J. Biochem. 214:577-585(1993) [PubMed] [Europe PMC] [Abstract] Cited for: TRANSPORT INTO MITOCHONDRIA.
[7]	"Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components." Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K. Eur. J. Biochem. 269:5203-5214(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, MASS SPECTROMETRY.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X53840 Genomic DNA. Translation: CAA37833.1. Z27116 Genomic DNA. Translation: CAA81636.1. Z28310 Genomic DNA. Translation: CAA82164.1. BK006944 Genomic DNA. Translation: DAA09235.1.
PIR	S38163.
RefSeq	NP_013011.1. NM_001179875.1.
3D structure databases
ProteinModelPortal	P22354.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6793N.
IntAct	P22354. 7 interactions.
MINT	MINT-667597.
STRING	4932.YKR085C.
Proteomic databases
PaxDb	P22354.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKR085C; YKR085C; YKR085C.
GeneID	853960.
KEGG	sce:YKR085C.
Organism-specific databases
CYGD	YKR085c.
SGD	S000001793. MRPL20.
Phylogenomic databases
eggNOG	NOG40516.
HOGENOM	HOG000001097.
OMA	SASNYKG.
OrthoDB	EOG4H75MR.
Gene expression databases
Genevestigator	P22354.
GermOnline	YKR085C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR024388. Ribosomal_L20_mt. [Graphical view]
Pfam	PF12824. MRP-L20. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975383.

Entry information

Entry name

RM20_YEAST

Accession

Primary (citable) accession number: P22354
Secondary accession number(s): D6VXE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program