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Protein

54S ribosomal protein L20, mitochondrial

Gene

MRPL20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.2 Publications

Miscellaneous

Present with 350 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • mitochondrial translation Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-32048-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
54S ribosomal protein L20, mitochondrial
Alternative name(s):
Mitochondrial large ribosomal subunit protein mL581 Publication
YmL20
Gene namesi
Name:MRPL20
Ordered Locus Names:YKR085C
ORF Names:YKR405
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR085C.
SGDiS000001793. MRPL20.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial large ribosomal subunit Source: SGD

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 18Mitochondrion1 PublicationAdd BLAST18
ChainiPRO_000003057319 – 19554S ribosomal protein L20, mitochondrialAdd BLAST177

Proteomic databases

MaxQBiP22354.
PRIDEiP22354.

Interactioni

Subunit structurei

Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature yeast 74S mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S small subunit contains a 15S ribosomal RNA (15S mt-rRNA) and 34 different proteins. The 54S large subunit contains a 21S rRNA (21S mt-rRNA) and 46 different proteins.3 Publications

Protein-protein interaction databases

BioGridi34216. 94 interactors.
DIPiDIP-6793N.
IntActiP22354. 9 interactors.
MINTiMINT-667597.
STRINGi4932.YKR085C.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Belectron microscopy3.20a1-195[»]
5MRCelectron microscopy3.25a19-195[»]
5MREelectron microscopy3.75a19-195[»]
5MRFelectron microscopy4.97a19-195[»]
ProteinModelPortaliP22354.
SMRiP22354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi113 – 129Arg/Lys-rich (basic)Add BLAST17

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000001097.
InParanoidiP22354.
OMAiSASNYKG.
OrthoDBiEOG092C5SQ9.

Family and domain databases

InterProiView protein in InterPro
IPR024388. Ribosomal_L20_mt.
PfamiView protein in Pfam
PF12824. MRP-L20. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22354-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIGRGVCCRS FHTAGSAWKQ FGFPKTQVTT IYNKTKSASN YKGYLKHRDA
60 70 80 90 100
PGMYYQPSES IATGSVNSET IPRSFMAASD PRRGLDMPVQ STKAKQCPNV
110 120 130 140 150
LVGKSTVNGK TYHLGPQEID EIRKLRLDNP QKYTRKFLAA KYGISPLFVS
160 170 180 190
MVSKPSEQHV QIMESRLQEI QSRWKEKRRI AREDRKRRKL LWYQA
Length:195
Mass (Da):22,392
Last modified:June 1, 1994 - v2
Checksum:i3165663C8C19EC60
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti187R → H in CAA37833 (PubMed:2183197).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53840 Genomic DNA. Translation: CAA37833.1.
Z27116 Genomic DNA. Translation: CAA81636.1.
Z28310 Genomic DNA. Translation: CAA82164.1.
BK006944 Genomic DNA. Translation: DAA09235.1.
PIRiS38163.
RefSeqiNP_013011.1. NM_001179875.1.

Genome annotation databases

EnsemblFungiiYKR085C; YKR085C; YKR085C.
GeneIDi853960.
KEGGisce:YKR085C.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRM20_YEAST
AccessioniPrimary (citable) accession number: P22354
Secondary accession number(s): D6VXE5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: June 1, 1994
Last modified: July 5, 2017
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names