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P22354

- RM20_YEAST

UniProt

P22354 - RM20_YEAST

Protein

54S ribosomal protein L20, mitochondrial

Gene

MRPL20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. mitochondrial translation Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32048-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    54S ribosomal protein L20, mitochondrial
    Alternative name(s):
    YmL20
    Gene namesi
    Name:MRPL20
    Ordered Locus Names:YKR085C
    ORF Names:YKR405
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKR085c.
    SGDiS000001793. MRPL20.

    Subcellular locationi

    Mitochondrion 2 Publications

    GO - Cellular componenti

    1. mitochondrial large ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1818Mitochondrion1 PublicationAdd
    BLAST
    Chaini19 – 19517754S ribosomal protein L20, mitochondrialPRO_0000030573Add
    BLAST

    Proteomic databases

    MaxQBiP22354.
    PaxDbiP22354.

    Expressioni

    Gene expression databases

    GenevestigatoriP22354.

    Interactioni

    Subunit structurei

    Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S).2 Publications

    Protein-protein interaction databases

    BioGridi34216. 29 interactions.
    DIPiDIP-6793N.
    IntActiP22354. 9 interactions.
    MINTiMINT-667597.
    STRINGi4932.YKR085C.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VW4electron microscopy3.20a1-195[»]
    ProteinModelPortaliP22354.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi113 – 12917Arg/Lys-rich (basic)Add
    BLAST

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiNOG40516.
    HOGENOMiHOG000001097.
    OMAiHTAGSAW.
    OrthoDBiEOG769ZXC.

    Family and domain databases

    InterProiIPR024388. Ribosomal_L20_mt.
    [Graphical view]
    PfamiPF12824. MRP-L20. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P22354-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIGRGVCCRS FHTAGSAWKQ FGFPKTQVTT IYNKTKSASN YKGYLKHRDA    50
    PGMYYQPSES IATGSVNSET IPRSFMAASD PRRGLDMPVQ STKAKQCPNV 100
    LVGKSTVNGK TYHLGPQEID EIRKLRLDNP QKYTRKFLAA KYGISPLFVS 150
    MVSKPSEQHV QIMESRLQEI QSRWKEKRRI AREDRKRRKL LWYQA 195
    Length:195
    Mass (Da):22,392
    Last modified:June 1, 1994 - v2
    Checksum:i3165663C8C19EC60
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti187 – 1871R → H in CAA37833. (PubMed:2183197)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53840 Genomic DNA. Translation: CAA37833.1.
    Z27116 Genomic DNA. Translation: CAA81636.1.
    Z28310 Genomic DNA. Translation: CAA82164.1.
    BK006944 Genomic DNA. Translation: DAA09235.1.
    PIRiS38163.
    RefSeqiNP_013011.1. NM_001179875.1.

    Genome annotation databases

    EnsemblFungiiYKR085C; YKR085C; YKR085C.
    GeneIDi853960.
    KEGGisce:YKR085C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53840 Genomic DNA. Translation: CAA37833.1 .
    Z27116 Genomic DNA. Translation: CAA81636.1 .
    Z28310 Genomic DNA. Translation: CAA82164.1 .
    BK006944 Genomic DNA. Translation: DAA09235.1 .
    PIRi S38163.
    RefSeqi NP_013011.1. NM_001179875.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VW4 electron microscopy 3.20 a 1-195 [» ]
    ProteinModelPortali P22354.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34216. 29 interactions.
    DIPi DIP-6793N.
    IntActi P22354. 9 interactions.
    MINTi MINT-667597.
    STRINGi 4932.YKR085C.

    Proteomic databases

    MaxQBi P22354.
    PaxDbi P22354.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKR085C ; YKR085C ; YKR085C .
    GeneIDi 853960.
    KEGGi sce:YKR085C.

    Organism-specific databases

    CYGDi YKR085c.
    SGDi S000001793. MRPL20.

    Phylogenomic databases

    eggNOGi NOG40516.
    HOGENOMi HOG000001097.
    OMAi HTAGSAW.
    OrthoDBi EOG769ZXC.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32048-MONOMER.

    Miscellaneous databases

    NextBioi 975383.

    Gene expression databases

    Genevestigatori P22354.

    Family and domain databases

    InterProi IPR024388. Ribosomal_L20_mt.
    [Graphical view ]
    Pfami PF12824. MRP-L20. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of nuclear genes for two mitochondrial ribosomal proteins in Saccharomyces cerevisiae."
      Kitakawa M., Grohmann L., Graack H.-R., Isono K.
      Nucleic Acids Res. 18:1521-1529(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 19-42.
    2. "The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames."
      Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G., Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F.
      Yeast 10:231-245(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria."
      Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S., Kitakawa M.
      FEBS Lett. 284:51-56(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 111-123, SUBUNIT.
      Strain: 07173.
    6. "Mitochondrial transport of mitoribosomal proteins, YmL8 and YmL20, in Saccharomyces cerevisiae."
      Matsushita Y., Isono K.
      Eur. J. Biochem. 214:577-585(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: TRANSPORT INTO MITOCHONDRIA.
    7. "Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components."
      Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.
      Eur. J. Biochem. 269:5203-5214(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.

    Entry informationi

    Entry nameiRM20_YEAST
    AccessioniPrimary (citable) accession number: P22354
    Secondary accession number(s): D6VXE5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 350 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3