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P22354

- RM20_YEAST

UniProt

P22354 - RM20_YEAST

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Protein

54S ribosomal protein L20, mitochondrial

Gene

MRPL20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. mitochondrial translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-32048-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
54S ribosomal protein L20, mitochondrial
Alternative name(s):
YmL20
Gene namesi
Name:MRPL20
Ordered Locus Names:YKR085C
ORF Names:YKR405
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKR085c.
SGDiS000001793. MRPL20.

Subcellular locationi

Mitochondrion 2 Publications

GO - Cellular componenti

  1. mitochondrial large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1818Mitochondrion1 PublicationAdd
BLAST
Chaini19 – 19517754S ribosomal protein L20, mitochondrialPRO_0000030573Add
BLAST

Proteomic databases

MaxQBiP22354.
PaxDbiP22354.

Expressioni

Gene expression databases

GenevestigatoriP22354.

Interactioni

Subunit structurei

Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S).2 Publications

Protein-protein interaction databases

BioGridi34216. 30 interactions.
DIPiDIP-6793N.
IntActiP22354. 9 interactions.
MINTiMINT-667597.
STRINGi4932.YKR085C.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VW4electron microscopy3.20a1-195[»]
ProteinModelPortaliP22354.
SMRiP22354. Positions 20-195.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi113 – 12917Arg/Lys-rich (basic)Add
BLAST

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiNOG40516.
HOGENOMiHOG000001097.
InParanoidiP22354.
OMAiHTAGSAW.
OrthoDBiEOG769ZXC.

Family and domain databases

InterProiIPR024388. Ribosomal_L20_mt.
[Graphical view]
PfamiPF12824. MRP-L20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22354-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIGRGVCCRS FHTAGSAWKQ FGFPKTQVTT IYNKTKSASN YKGYLKHRDA
60 70 80 90 100
PGMYYQPSES IATGSVNSET IPRSFMAASD PRRGLDMPVQ STKAKQCPNV
110 120 130 140 150
LVGKSTVNGK TYHLGPQEID EIRKLRLDNP QKYTRKFLAA KYGISPLFVS
160 170 180 190
MVSKPSEQHV QIMESRLQEI QSRWKEKRRI AREDRKRRKL LWYQA
Length:195
Mass (Da):22,392
Last modified:June 1, 1994 - v2
Checksum:i3165663C8C19EC60
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti187 – 1871R → H in CAA37833. (PubMed:2183197)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53840 Genomic DNA. Translation: CAA37833.1.
Z27116 Genomic DNA. Translation: CAA81636.1.
Z28310 Genomic DNA. Translation: CAA82164.1.
BK006944 Genomic DNA. Translation: DAA09235.1.
PIRiS38163.
RefSeqiNP_013011.1. NM_001179875.1.

Genome annotation databases

EnsemblFungiiYKR085C; YKR085C; YKR085C.
GeneIDi853960.
KEGGisce:YKR085C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53840 Genomic DNA. Translation: CAA37833.1 .
Z27116 Genomic DNA. Translation: CAA81636.1 .
Z28310 Genomic DNA. Translation: CAA82164.1 .
BK006944 Genomic DNA. Translation: DAA09235.1 .
PIRi S38163.
RefSeqi NP_013011.1. NM_001179875.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1VW4 electron microscopy 3.20 a 1-195 [» ]
ProteinModelPortali P22354.
SMRi P22354. Positions 20-195.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34216. 30 interactions.
DIPi DIP-6793N.
IntActi P22354. 9 interactions.
MINTi MINT-667597.
STRINGi 4932.YKR085C.

Proteomic databases

MaxQBi P22354.
PaxDbi P22354.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKR085C ; YKR085C ; YKR085C .
GeneIDi 853960.
KEGGi sce:YKR085C.

Organism-specific databases

CYGDi YKR085c.
SGDi S000001793. MRPL20.

Phylogenomic databases

eggNOGi NOG40516.
HOGENOMi HOG000001097.
InParanoidi P22354.
OMAi HTAGSAW.
OrthoDBi EOG769ZXC.

Enzyme and pathway databases

BioCyci YEAST:G3O-32048-MONOMER.

Miscellaneous databases

NextBioi 975383.

Gene expression databases

Genevestigatori P22354.

Family and domain databases

InterProi IPR024388. Ribosomal_L20_mt.
[Graphical view ]
Pfami PF12824. MRP-L20. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of nuclear genes for two mitochondrial ribosomal proteins in Saccharomyces cerevisiae."
    Kitakawa M., Grohmann L., Graack H.-R., Isono K.
    Nucleic Acids Res. 18:1521-1529(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 19-42.
  2. "The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames."
    Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G., Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F.
    Yeast 10:231-245(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria."
    Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S., Kitakawa M.
    FEBS Lett. 284:51-56(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 111-123, SUBUNIT.
    Strain: 07173.
  6. "Mitochondrial transport of mitoribosomal proteins, YmL8 and YmL20, in Saccharomyces cerevisiae."
    Matsushita Y., Isono K.
    Eur. J. Biochem. 214:577-585(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: TRANSPORT INTO MITOCHONDRIA.
  7. "Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components."
    Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.
    Eur. J. Biochem. 269:5203-5214(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.

Entry informationi

Entry nameiRM20_YEAST
AccessioniPrimary (citable) accession number: P22354
Secondary accession number(s): D6VXE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 350 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3