P22353 (RM08_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
54S ribosomal protein L8, mitochondrial

Alternative name(s):
YmL8

Gene names

Name:	MRPL8
Ordered Locus Names:	YJL063C
ORF Names:	J1125, HRD238

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S).
Subcellular location	Mitochondrion Ref.8 Ref.10.
Miscellaneous	Although this protein has evidently no mitochondrial targeting pre-sequence, it is efficiently transported into mitochondria. Present with 3000 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Belongs to the ribosomal protein L17P family.

Ontologies

Keywords
Cellular component	Mitochondrion
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	mitochondrial genome maintenance Inferred from mutant phenotype Ref.1. Source: SGD mitochondrial translation Inferred by curator Ref.7. Source: SGD
Cellular component	mitochondrial large ribosomal subunit Inferred from physical interaction Ref.7. Source: SGD
Molecular function	structural constituent of ribosome Inferred from physical interaction Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 238	237	54S ribosomal protein L8, mitochondrial		PRO_0000175550
Experimental info
	Sequence conflict	2	1	T → G AA sequence Ref.1
	Sequence conflict	82	1	D → G in CAA37834. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P22353 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: A130EFD95E8719BA	FASTA	238	26,945
	10 20 30 40 50 60 MTVGIARKLS RDKAHRDALL KNLACQLFQH ESIVSTHAKC KEASRVAERI ITWTKRAITT 70 80 90 100 110 120 SNSVAQAELK SQIQSQLFLA GDNRKLMKRL FSEIAPRYLE RPGGYTRVLR LEPRANDSAP 130 140 150 160 170 180 QSVLELVDSP VMSESHTVNR GNLKMWLLVK SVINDDANQL PHNPLTLQNL HKVAKFKAEA 190 200 210 220 230 QLHGEIMLIK QVLLKEMSLP YDEALENERT QALLKEVYSS SLPKKTKKPS SYVMVPRP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of nuclear genes for two mitochondrial ribosomal proteins in Saccharomyces cerevisiae." Kitakawa M., Grohmann L., Graack H.-R., Isono K. Nucleic Acids Res. 18:1521-1529(1990) [PubMed: 2183197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-28. Strain: 07173.
[2]	"Sequence of a 17.1 kb DNA fragment from chromosome X of Saccharomyces cerevisiae includes the mitochondrial ribosomal protein L8." Vandenbol M., Durand P., Dion C., Portetelle D., Hilger F. Yeast 11:57-60(1995) [PubMed: 7762302] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Mitochondrial transport of mitoribosomal proteins, YmL8 and YmL20, in Saccharomyces cerevisiae." Matsushita Y., Isono K. Eur. J. Biochem. 214:577-585(1993) [PubMed: 8513807] [Abstract] Cited for: TRANSPORT INTO MITOCHONDRIA.
[7]	"Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components." Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K. Eur. J. Biochem. 269:5203-5214(2002) [PubMed: 12392552] [Abstract] Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, MASS SPECTROMETRY.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X53841 Genomic DNA. Translation: CAA37834.1. Z34288 Genomic DNA. Translation: CAA84060.1. Z49338 Genomic DNA. Translation: CAA89354.1. AY558249 Genomic DNA. Translation: AAS56575.1. BK006943 Genomic DNA. Translation: DAA08734.1.
PIR	S50809.
RefSeq	NP_012472.1. NM_001181496.1.
3D structure databases
ProteinModelPortal	P22353.
SMR	P22353. Positions 1-128.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5672N.
IntAct	P22353. 8 interactions.
MINT	MINT-497599.
STRING	P22353.
Proteomic databases
PeptideAtlas	P22353.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL063C; YJL063C; YJL063C.
GeneID	853382.
KEGG	sce:YJL063C.
NMPDR	fig|4932.3.peg.3443.
Organism-specific databases
SGD	S000003599. MRPL8.
Phylogenomic databases
eggNOG	fuNOG10459.
GeneTree	EFGT00050000005356.
HOGENOM	HBG202194.
OMA	IRHEYIV.
OrthoDB	EOG4GMZ6J.
Gene expression databases
ArrayExpress	P22353.
Genevestigator	P22353.
GermOnline	YJL063C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000456. Ribosomal_L17. [Graphical view]
Gene3D	G3DSA:3.90.1030.10. Ribosomal_L17. 1 hit.
PANTHER	PTHR14413. Ribosomal_L17. 1 hit.
Pfam	PF01196. Ribosomal_L17. 1 hit. [Graphical view]
SUPFAM	SSF64263. Ribosomal_L17. 1 hit.
TIGRFAMs	TIGR00059. L17. 1 hit.
PROSITE	PS01167. RIBOSOMAL_L17. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973837.

Entry information

Entry name

RM08_YEAST

Accession

Primary (citable) accession number: P22353
Secondary accession number(s): D6VWB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 108 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program