Reviewed,
UniProtKB/Swiss-Prot P22352 (GPX3_HUMAN)
Last modified
November 25, 2008.
Version 96.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Glutathione peroxidase 3 EC=1.11.1.9 Alternative name(s): GSHPx-3 Short name=GPx-3 Extracellular glutathione peroxidase Plasma glutathione peroxidase GSHPx-P Short name=GPx-P | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 226 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. |
| Catalytic activity | 2 glutathione + H(2)O(2) = glutathione disulfide + 2 H(2)O. |
| Subunit structure | Homotetramer. |
| Subcellular location | |
| Tissue specificity | Secreted in plasma. |
| Post-translational modification | The N-terminus is blocked. |
| Sequence similarities | Belongs to the glutathione peroxidase family. |
Ontologies
Keywords | |
|---|---|
| Cellular component | Secreted |
| Coding sequence diversity | Polymorphism Selenocysteine |
| Domain | Signal |
| Ligand | Selenium |
| Molecular function | Oxidoreductase Peroxidase |
| Technical term | 3D-structure Direct protein sequencing |
Gene Ontology (GO) | |
| Biological process | hydrogen peroxide catabolic process Traceable author statement. Source: UniProtKB oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW protein homotetramerizationInferred from direct assay. Source: UniProtKB response to lipid hydroperoxideTraceable author statement. Source: UniProtKB |
| Cellular component | extracellular space Ref.7 Inferred from direct assay. Source: UniProtKB |
| Molecular function | glutathione peroxidase activity Ref.7 Inferred from direct assay. Source: UniProtKB selenium bindingInferred from direct assay. Source: UniProtKB transcription factor binding Ref.3Traceable author statement. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||||||||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 20 | 20 | Potential | |||||||||||||||||||||||||||||||||||||||||||
| Chain | 21 – 226 | 206 | Glutathione peroxidase 3 | PRO_0000013062 | ||||||||||||||||||||||||||||||||||||||||||
Sites | ||||||||||||||||||||||||||||||||||||||||||||||
| Active site | 73 | 1 | ||||||||||||||||||||||||||||||||||||||||||||
Amino acid modifications | ||||||||||||||||||||||||||||||||||||||||||||||
| Non-standard residue | 73 | 1 | Selenocysteine | |||||||||||||||||||||||||||||||||||||||||||
Natural variations | ||||||||||||||||||||||||||||||||||||||||||||||
| Natural variant | 128 | 1 | F → L | VAR_020943 | ||||||||||||||||||||||||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||||||||||||||||||||||||
| Helix | 40 – 42 | 3 | ||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 44 – 47 | 4 | ||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 53 – 55 | 3 | ||||||||||||||||||||||||||||||||||||||||||||
| Helix | 56 – 59 | 4 | ||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 62 – 69 | 8 | ||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 71 – 73 | 3 | ||||||||||||||||||||||||||||||||||||||||||||
| Turn | 74 – 77 | 4 | ||||||||||||||||||||||||||||||||||||||||||||
| Helix | 78 – 89 | 12 | ||||||||||||||||||||||||||||||||||||||||||||
| Helix | 90 – 92 | 3 | ||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 94 – 100 | 7 | ||||||||||||||||||||||||||||||||||||||||||||
| Helix | 112 – 114 | 3 | ||||||||||||||||||||||||||||||||||||||||||||
| Helix | 115 – 121 | 7 | ||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 131 – 135 | 5 | ||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 140 – 142 | 3 | ||||||||||||||||||||||||||||||||||||||||||||
| Helix | 147 – 155 | 9 | ||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 161 – 164 | 4 | ||||||||||||||||||||||||||||||||||||||||||||
| Helix | 166 – 168 | 3 | ||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 172 – 175 | 4 | ||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 185 – 188 | 4 | ||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 194 – 198 | 5 | ||||||||||||||||||||||||||||||||||||||||||||
| Helix | 204 – 223 | 20 | ||||||||||||||||||||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Primary structure of human plasma glutathione peroxidase deduced from cDNA sequences." Takahashi K., Akasaka M., Yamamoto Y., Kobayashi C., Mizoguchi J., Koyama J. J. Biochem. 108:145-148(1990) [PubMed: 2229017] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Fetal liver and Placenta. |
| [2] | "Expression of plasma glutathione peroxidase in human liver in addition to kidney, heart, lung, and breast in humans and rodents." Chu F.-F., Esworthy R.S., Doroshow J.H., Doan K., Liu X.F. Blood 79:3233-3238(1992) [PubMed: 1339300] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney. |
| [3] | "The human plasma glutathione peroxidase-encoding gene: organization, sequence and localization to chromosome 5q32." Yoshimura S., Suemizu H., Taniguchi Y., Arimori K., Kawabe N., Moriuchi T. Gene 145:293-297(1994) [PubMed: 8056346] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Lymphocyte. |
| [4] | "Differential induction of extracellular glutathione peroxidase and nitric oxide synthase 2 in airways of healthy individuals exposed to 100% O(2) or cigarette smoke." Comhair S.A.A., Thomassen M.J., Erzurum S.C. Am. J. Respir. Cell Mol. Biol. 23:350-354(2000) [PubMed: 10970826] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [5] | "NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu)." Rieder M.J., Livingston R.J., Daniels M.R., Chung M.-W., Miyamoto K.E., Nguyen C.P., Nguyen D.A., Poel C.L., Robertson P.D., Schackwitz W.S., Sherwood J.K., Witrak L.A., Nickerson D.A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LEU-128. |
| [6] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Duodenum, Lung and Pancreas. |
| [7] | "Characterization and partial amino acid sequence of human plasma glutathione peroxidase." Esworthy R.S., Chu F.-F., Paxton R.J., Akman S., Doroshow J.H. Arch. Biochem. Biophys. 286:330-336(1991) [PubMed: 1897960] [Abstract] Cited for: PROTEIN SEQUENCE OF 107-138 AND 148-175, CHARACTERIZATION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| D00632 mRNA. Translation: BAA00525.1. X58295 mRNA. Translation: CAA41228.1. D16360 Genomic DNA. Translation: BAA03862.1. D16361 Genomic DNA. Translation: BAA03863.2. D16362 Genomic DNA. Translation: BAA03864.1. AF217787 mRNA. Translation: AAF43005.1. AY310878 Genomic DNA. Translation: AAP50261.1. BC013601 mRNA. Translation: AAH13601.1. BC035841 mRNA. Translation: AAH35841.1. BC050378 mRNA. Translation: AAH50378.2. | |||||||||||||
| PIR | JQ0476. I53822. | ||||||||||||
| RefSeq | NP_002075.2. | ||||||||||||
| UniGene | Hs.386793 Hs.663430 | ||||||||||||
3D structure databases | |||||||||||||
| |||||||||||||
| ModBase | Search... | ||||||||||||
Protein family/group databases | |||||||||||||
| PeroxiBase | 3602. HsGPx03. | ||||||||||||
Polymorphism databases | |||||||||||||
| NIEHS-SNPs | Search... | ||||||||||||
2-D gel databases | |||||||||||||
| SWISS-2DPAGE | P22352. | ||||||||||||
| REPRODUCTION-2DPAGE | IPI00026199. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENSG00000211445. Homo sapiens. [Contig view] | ||||||||||||
| GeneID | 2878. | ||||||||||||
| KEGG | hsa:2878. | ||||||||||||
Organism-specific databases | |||||||||||||
| HGNC | HGNC:4555. GPX3. | ||||||||||||
| MIM | 138321. gene. | ||||||||||||
| PharmGKB | PA28951. | ||||||||||||
| GenAtlas | Search... | ||||||||||||
| GeneCards | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOGENOM | P22352. | ||||||||||||
| HOVERGEN | P22352. | ||||||||||||
Enzyme and pathway databases | |||||||||||||
| BioCyc | MetaCyc:MON-9873. | ||||||||||||
Gene expression databases | |||||||||||||
| CleanEx | HS_GPX3. | ||||||||||||
| GermOnline | ENSG00000211445. Homo sapiens. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR000889. Glut_peroxidase. IPR012335. Thioredoxin_fold. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit. | ||||||||||||
| PANTHER | PTHR11592. Glut_peroxidase. 1 hit. | ||||||||||||
| Pfam | PF00255. GSHPx. 1 hit. [Graphical view] | ||||||||||||
| PIRSF | PIRSF000303. Glutathion_perox. 1 hit. | ||||||||||||
| PRINTS | PR01011. GLUTPROXDASE. | ||||||||||||
| PROSITE | PS00460. GLUTATHIONE_PEROXID_1. 1 hit. PS00763. GLUTATHIONE_PEROXID_2. 1 hit. PS51355. GLUTATHIONE_PEROXID_3. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| DrugBank | DB00143. Glutathione. | ||||||||||||
| NextBio | 11363. | ||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | GPX3_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P22352 Secondary accession number(s): O43787 Q9UEL1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 5 Human chromosome 5: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


