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P22337

- STAD_RICCO

UniProt

P22337 - STAD_RICCO

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Protein
Acyl-[acyl-carrier-protein] desaturase, chloroplastic
Gene
N/A
Organism
Ricinus communis (Castor bean)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Converts stearoyl-ACP to oleoyl-ACP by introduction of a cis double bond between carbons Delta9 and Delta(10) of the acyl chain.

Catalytic activityi

Stearoyl-[acyl-carrier-protein] + reduced acceptor + O2 = oleoyl-[acyl-carrier-protein] + acceptor + 2 H2O.2 Publications

Cofactori

Binds 2 iron ions per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi138 – 1381Iron 1
Metal bindingi176 – 1761Iron 1
Metal bindingi176 – 1761Iron 2
Metal bindingi179 – 1791Iron 1; via pros nitrogen
Metal bindingi229 – 2291Iron 2
Metal bindingi262 – 2621Iron 1
Metal bindingi262 – 2621Iron 2
Metal bindingi265 – 2651Iron 2; via pros nitrogen

GO - Molecular functioni

  1. acyl-[acyl-carrier-protein] desaturase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. fatty acid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.14.19.2. 1204.
SABIO-RKP22337.
UniPathwayiUPA00199.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-[acyl-carrier-protein] desaturase, chloroplastic (EC:1.14.19.2)
Alternative name(s):
Delta(9) stearoyl-acyl carrier protein desaturase
Stearoyl-ACP desaturase
OrganismiRicinus communis (Castor bean)
Taxonomic identifieri3988 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesEuphorbiaceaeAcalyphoideaeAcalypheaeRicinus

Subcellular locationi

Plastidchloroplast. Plastid
Note: In green tissue, found in chloroplasts. In non-photosynthetic tissue, found in plastids.

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi232 – 2321T → D or E: Decreases desaturase activity and increases oxidase activity. 1 Publication
Mutagenesisi313 – 3131D → K or R: Decreases Delta(9) desaturase activity and increases Delta(4) desaturase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3333Chloroplast
Add
BLAST
Chaini34 – 396363Acyl-[acyl-carrier-protein] desaturase, chloroplastic
PRO_0000007137Add
BLAST

Proteomic databases

PRIDEiP22337.

Expressioni

Tissue specificityi

Higher levels in developing seeds than in leaf and root tissues.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

DIPiDIP-60379N.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni67 – 693
Helixi70 – 756
Helixi77 – 837
Helixi85 – 873
Helixi91 – 933
Helixi97 – 1004
Helixi109 – 12113
Helixi125 – 13915
Helixi141 – 1488
Turni152 – 1543
Beta strandi157 – 1604
Helixi164 – 19027
Beta strandi191 – 1933
Helixi195 – 20814
Helixi218 – 24528
Helixi249 – 27931
Helixi281 – 29414
Turni299 – 3024
Helixi311 – 32212
Helixi327 – 34115
Helixi343 – 3453
Helixi351 – 37222
Helixi376 – 3794
Beta strandi383 – 3853
Helixi387 – 3893
Beta strandi393 – 3953

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AFRX-ray2.40A/B/C/D/E/F52-396[»]
1OQ4X-ray2.40A/B/C/D/E/F34-396[»]
1OQ7X-ray3.20A/B/C/D/E/F34-396[»]
1OQ9X-ray2.40A34-396[»]
1OQBX-ray2.80A/B/C/D/E/F34-396[»]
2J2FX-ray2.65A/B/C/D/E/F34-396[»]
2XZ0X-ray3.00A/B/C34-396[»]
2XZ1X-ray3.35A/B34-396[»]
ProteinModelPortaliP22337.
SMRiP22337. Positions 51-396.

Miscellaneous databases

EvolutionaryTraceiP22337.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK03921.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR005067. Fatty_acid_desaturase-2.
IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR005803. Stearoyl-ACP_desaturase_CS.
[Graphical view]
PfamiPF03405. FA_desaturase_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000346. Dlt9_acylACP_des. 1 hit.
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00574. FATTY_ACID_DESATUR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22337-1 [UniParc]FASTAAdd to Basket

« Hide

MALKLNPFLS QTQKLPSFAL PPMASTRSPK FYMASTLKSG SKEVENLKKP    50
FMPPREVHVQ VTHSMPPQKI EIFKSLDNWA EENILVHLKP VEKCWQPQDF 100
LPDPASDGFD EQVRELRERA KEIPDDYFVV LVGDMITEEA LPTYQTMLNT 150
LDGVRDETGA SPTSWAIWTR AWTAEENRHG DLLNKYLYLS GRVDMRQIEK 200
TIQYLIGSGM DPRTENSPYL GFIYTSFQER ATFISHGNTA RQAKEHGDIK 250
LAQICGTIAA DEKRHETAYT KIVEKLFEID PDGTVLAFAD MMRKKISMPA 300
HLMYDGRDDN LFDHFSAVAQ RLGVYTAKDY ADILEFLVGR WKVDKLTGLS 350
AEGQKAQDYV CRLPPRIRRL EERAQGRAKE APTMPFSWIF DRQVKL 396
Length:396
Mass (Da):45,371
Last modified:August 1, 1991 - v1
Checksum:iE50D4725996392AF
GO

Sequence cautioni

The sequence AAA74692.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59857 mRNA. Translation: AAA74692.1. Different initiation.
X56508 mRNA. Translation: CAA39859.1.
PIRiS16463. OHCSAD.
RefSeqiXP_002531889.1. XM_002531843.1.

Genome annotation databases

GeneIDi8271760.
KEGGircu:RCOM_1238330.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59857 mRNA. Translation: AAA74692.1 . Different initiation.
X56508 mRNA. Translation: CAA39859.1 .
PIRi S16463. OHCSAD.
RefSeqi XP_002531889.1. XM_002531843.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AFR X-ray 2.40 A/B/C/D/E/F 52-396 [» ]
1OQ4 X-ray 2.40 A/B/C/D/E/F 34-396 [» ]
1OQ7 X-ray 3.20 A/B/C/D/E/F 34-396 [» ]
1OQ9 X-ray 2.40 A 34-396 [» ]
1OQB X-ray 2.80 A/B/C/D/E/F 34-396 [» ]
2J2F X-ray 2.65 A/B/C/D/E/F 34-396 [» ]
2XZ0 X-ray 3.00 A/B/C 34-396 [» ]
2XZ1 X-ray 3.35 A/B 34-396 [» ]
ProteinModelPortali P22337.
SMRi P22337. Positions 51-396.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-60379N.

Proteomic databases

PRIDEi P22337.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 8271760.
KEGGi rcu:RCOM_1238330.

Phylogenomic databases

KOi K03921.

Enzyme and pathway databases

UniPathwayi UPA00199 .
BRENDAi 1.14.19.2. 1204.
SABIO-RK P22337.

Miscellaneous databases

EvolutionaryTracei P22337.

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR005067. Fatty_acid_desaturase-2.
IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR005803. Stearoyl-ACP_desaturase_CS.
[Graphical view ]
Pfami PF03405. FA_desaturase_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000346. Dlt9_acylACP_des. 1 hit.
SUPFAMi SSF47240. SSF47240. 1 hit.
PROSITEi PS00574. FATTY_ACID_DESATUR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Stearoyl-acyl-carrier-protein desaturase from higher plants is structurally unrelated to the animal and fungal homologs."
    Shanklin J., Somerville C.R.
    Proc. Natl. Acad. Sci. U.S.A. 88:2510-2514(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Nucleotide sequence of a complementary DNA clone encoding stearoyl-acyl carrier protein desaturase from castor bean, Ricinus communis."
    Knutzon D.S., Scherer D.E., Schreckengost W.E.
    Plant Physiol. 96:344-345(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Endosperm.
  3. "Crystal structure of delta9 stearoyl-acyl carrier protein desaturase from castor seed and its relationship to other di-iron proteins."
    Lindqvist Y., Huang W., Schneider G., Shanklin J.
    EMBO J. 15:4081-4092(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 52-396 IN COMPLEX WITH IRON.
  4. "Azide and acetate complexes plus two iron-depleted crystal structures of the di-iron enzyme delta9 stearoyl-acyl carrier protein desaturase. Implications for oxygen activation and catalytic intermediates."
    Moche M., Shanklin J., Ghoshal A., Lindqvist Y.
    J. Biol. Chem. 278:25072-25080(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 34-396 IN COMPLEX WITH IRON.
  5. "A single mutation in the castor Delta9-18:0-desaturase changes reaction partitioning from desaturation to oxidase chemistry."
    Guy J.E., Abreu I.A., Moche M., Lindqvist Y., Whittle E., Shanklin J.
    Proc. Natl. Acad. Sci. U.S.A. 103:17220-17224(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) OF 34-396 IN COMPLEX WITH IRON, MUTAGENESIS OF THR-232, CATALYTIC ACTIVITY.
  6. "Remote control of regioselectivity in acyl-acyl carrier protein-desaturases."
    Guy J.E., Whittle E., Moche M., Lengqvist J., Lindqvist Y., Shanklin J.
    Proc. Natl. Acad. Sci. U.S.A. 108:16594-16599(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) OF 34-396, MUTAGENESIS OF ASP-313, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiSTAD_RICCO
AccessioniPrimary (citable) accession number: P22337
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: July 9, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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