Replication factor A protein 1 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P22336 (RFA1_YEAST)

Last modified November 24, 2009. Version 96. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Replication factor A protein 1
      Short name=RF-A protein 1
Alternative name(s):
    Replication protein A 69 kDa DNA-binding subunit
    Single-stranded DNA-binding protein
    DNA-binding protein BUF2

Gene names

Name:	RFA1
Synonyms:	BUF2, RPA1
Ordered Locus Names:	YAR007C
ORF Names:	FUN3

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication.
Subunit structure	Heterotrimer of 69, 36, and 13 kDa chains. The DNA-binding activity may reside exclusively on the 69 kDa subunit. Interacts with POB3. Ref.7
Subcellular location	Nucleus.
Post-translational modification	The N-terminus is blocked.
Miscellaneous	Present with 4100 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the replication factor A protein 1 family.

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Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA unwinding during replication Inferred from direct assay. Source: SGD double-strand break repair via homologous recombination Inferred from genetic interaction. Source: SGD establishment of protein localization Inferred from physical interaction. Source: SGD heteroduplex formation Inferred from direct assay. Source: SGD nucleotide-excision repair Inferred from direct assay. Source: SGD protein ubiquitination Inferred from mutant phenotype. Source: SGD reciprocal meiotic recombination Inferred from mutant phenotype. Source: SGD telomere maintenance via recombination Inferred from genetic interaction. Source: SGD telomere maintenance via telomerase Inferred from physical interaction. Source: SGD
Cellular component	DNA replication factor A complex Inferred from direct assay. Source: SGD chromosome, telomeric region Inferred from mutant phenotype. Source: SGD condensed nuclear chromosome Inferred from direct assay. Source: SGD cytoplasm Inferred from direct assay. Source: SGD
Molecular function	double-stranded DNA binding Inferred from direct assay. Source: SGD protein binding Ref.7 Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from direct assay. Source: SGD single-stranded DNA binding Inferred from direct assay. Source: SGD zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
POB3	Q04636	2	EBI-14971,EBI-27863

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 621

621

Replication factor A protein 1

PRO_0000097268

Regions

Zinc finger

486 – 508

C4-type Potential

Amino acid modifications

Modified residue

160

Phosphoserine Ref.9

Modified residue

178

Phosphoserine; by ATM or ATR Ref.9 Ref.8

Secondary structure

621

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P22336-1 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 7EB8DFA6910EF8A8
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FASTA

621

70,348

        10         20         30         40         50         60 
MSSVQLSRGD FHSIFTNKQR YDNPTGGVYQ VYNTRKSDGA NSNRKNLIMI SDGIYHMKAL 

        70         80         90        100        110        120 
LRNQAASKFQ SMELQRGDII RVIIAEPAIV RERKKYVLLV DDFELVQSRA DMVNQTSTFL 

       130        140        150        160        170        180 
DNYFSEHPNE TLKDEDITDS GNVANQTNAS NAGVPDMLHS NSNLNANERK FANENPNSQK 

       190        200        210        220        230        240 
TRPIFAIEQL SPYQNVWTIK ARVSYKGEIK TWHNQRGDGK LFNVNFLDTS GEIRATAFND 

       250        260        270        280        290        300 
FATKFNEILQ EGKVYYVSKA KLQPAKPQFT NLTHPYELNL DRDTVIEECF DESNVPKTHF 

       310        320        330        340        350        360 
NFIKLDAIQN QEVNSNVDVL GIIQTINPHF ELTSRAGKKF DRRDITIVDD SGFSISVGLW 

       370        380        390        400        410        420 
NQQALDFNLP EGSVAAIKGV RVTDFGGKSL SMGFSSTLIP NPEIPEAYAL KGWYDSKGRN 

       430        440        450        460        470        480 
ANFITLKQEP GMGGQSAASL TKFIAQRITI ARAQAENLGR SEKGDFFSVK AAISFLKVDN 

       490        500        510        520        530        540 
FAYPACSNEN CNKKVLEQPD GTWRCEKCDT NNARPNWRYI LTISIIDETN QLWLTLFDDQ 

       550        560        570        580        590        600 
AKQLLGVDAN TLMSLKEEDP NEFTKITQSI QMNEYDFRIR AREDTYNDQS RIRYTVANLH 

       610        620 
SLNYRAEADY LADELSKALL A

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Replication factor-A from Saccharomyces cerevisiae is encoded by three essential genes coordinately expressed at S phase." Brill S.J., Stillman B. Genes Dev. 5:1589-1600(1991) [PubMed: 1885001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 111-129 AND 495-503. Strain: ATCC 208353 / W303-1A.
[2]	"An essential Saccharomyces cerevisiae single-stranded DNA binding protein is homologous to the large subunit of human RP-A." Heyer W.-D., Rao M.R., Erdile L.F., Kelley T.J., Kolodner R.D. EMBO J. 9:2321-2329(1990) [PubMed: 2192864] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[3]	"Saccharomyces cerevisiae BUF protein binds to sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation." Luche R.M., Smart W.C., Marion T., Tillman M., Sumrada R.A., Cooper T.G. Mol. Cell. Biol. 13:5749-5761(1993) [PubMed: 8355713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region." Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H. Yeast 10:535-541(1994) [PubMed: 7941740] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed: 7731988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"The structure of the yFACT Pob3-M domain, its interaction with the DNA replication factor RPA, and a potential role in nucleosome deposition." Vandemark A.P., Blanksma M., Ferris E., Heroux A., Hill C.P., Formosa T. Mol. Cell 22:363-374(2006) [PubMed: 16678108] [Abstract] Cited for: INTERACTION WITH POB3.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-178, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-160 AND SER-178, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

X59748 Genomic DNA. Translation: CAA42420.1.
M60262 Genomic DNA. Translation: AAA34994.1.
S64901 Genomic DNA. Translation: AAB27889.1.
L22015 Genomic DNA. Translation: AAC04960.1.

PIR

S20145.

RefSeq

NP_009404.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YNX	NMR	-	A	181-294	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:2512N.

IntAct

P22336. 34 interactions.

STRING

P22336.

Proteomic databases

PeptideAtlas

P22336.

PRIDE

P22336.

Genome annotation databases

Ensembl

YAR007C; YAR007C; YAR007C; Saccharomyces cerevisiae. [Genome view]

GeneID

851266.

KEGG

sce:YAR007C.

NMPDR

fig|4932.3.peg.88.

Organism-specific databases

CYGD

YAR007c.

SGD

S000000065. RFA1.

Phylogenomic databases

HOGENOM

P22336.

OMA

QATMFNE

OrthoDB

EOG9QFXWF

Gene expression databases

ArrayExpress

P22336.

Genevestigator

P22336.

GermOnline

YAR007C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR004365. NA_bd_OB_tRNA-helicase.
IPR013955. Rep_factor-A_C.
IPR004591. Rpa1.
[Graphical view]

Gene3D

G3DSA:2.40.50.140. OB_NA_bd_sub. 4 hits.

Pfam

PF08646. Rep_fac-A_C. 1 hit.
PF01336. tRNA_anti. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00617. rpa1. 1 hit.

ProtoNet

Search...

Other Resources

NextBio

968234.

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Entry information

Entry name

RFA1_YEAST

Accession

Primary (citable) accession number: P22336
Secondary accession number(s): P38906

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	November 24, 2009
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents