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P22333

- ADD_ECOLI

UniProt

P22333 - ADD_ECOLI

Protein

Adenosine deaminase

Gene

add

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Adenosine + H2O = inosine + NH3.UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi12 – 121Zinc; catalyticUniRule annotation
    Metal bindingi14 – 141Zinc; catalyticUniRule annotation
    Binding sitei14 – 141SubstrateUniRule annotation
    Binding sitei16 – 161SubstrateUniRule annotation
    Binding sitei170 – 1701Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation
    Metal bindingi197 – 1971Zinc; catalyticUniRule annotation
    Active sitei200 – 2001Proton donorUniRule annotation
    Sitei221 – 2211Important for catalytic activityUniRule annotation
    Metal bindingi278 – 2781Zinc; catalyticUniRule annotation
    Binding sitei279 – 2791SubstrateUniRule annotation

    GO - Molecular functioni

    1. adenosine deaminase activity Source: EcoliWiki
    2. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. adenosine catabolic process Source: EcoliWiki
    2. cellular response to DNA damage stimulus Source: EcoliWiki
    3. hypoxanthine biosynthetic process Source: EcoliWiki
    4. hypoxanthine salvage Source: EcoliWiki
    5. inosine biosynthetic process Source: RefGenome
    6. purine nucleotide interconversion Source: EcoliWiki
    7. purine nucleotide salvage Source: EcoliWiki
    8. purine ribonucleoside monophosphate biosynthetic process Source: EcoliWiki

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Nucleotide metabolism

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciEcoCyc:ADENODEAMIN-MONOMER.
    ECOL316407:JW1615-MONOMER.
    MetaCyc:ADENODEAMIN-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenosine deaminaseUniRule annotation (EC:3.5.4.4UniRule annotation)
    Alternative name(s):
    Adenosine aminohydrolaseUniRule annotation
    Gene namesi
    Name:addUniRule annotation
    Ordered Locus Names:b1623, JW1615
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10030. add.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: RefGenome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 333333Adenosine deaminasePRO_0000194367Add
    BLAST

    Proteomic databases

    PaxDbiP22333.
    PRIDEiP22333.

    Expressioni

    Gene expression databases

    GenevestigatoriP22333.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-9056N.
    IntActiP22333. 15 interactions.
    MINTiMINT-1298977.
    STRINGi511145.b1623.

    Structurei

    3D structure databases

    ProteinModelPortaliP22333.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the adenosine and AMP deaminases family. Adenosine deaminase subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1816.
    HOGENOMiHOG000218815.
    KOiK01488.
    OMAiDWMVAVL.
    OrthoDBiEOG6BPDDD.
    PhylomeDBiP22333.

    Family and domain databases

    HAMAPiMF_00540. A_deaminase.
    InterProiIPR006650. A/AMP_deam_AS.
    IPR001365. A/AMP_deaminase_dom.
    IPR028893. A_deaminase.
    IPR006330. Ado/ade_deaminase.
    [Graphical view]
    PfamiPF00962. A_deaminase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01430. aden_deam. 1 hit.
    PROSITEiPS00485. A_DEAMINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P22333-1 [UniParc]FASTAAdd to Basket

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    MIDTTLPLTD IHRHLDGNIR PQTILELGRQ YNISLPAQSL ETLIPHVQVI    50
    ANEPDLVSFL TKLDWGVKVL ASLDACRRVA FENIEDAARH GLHYVELRFS 100
    PGYMAMAHQL PVAGVVEAVI DGVREGCRTF GVQAKLIGIM SRTFGEAACQ 150
    QELEAFLAHR DQITALDLAG DELGFPGSLF LSHFNRARDA GWHITVHAGE 200
    AAGPESIWQA IRELGAERIG HGVKAIEDRA LMDFLAEQQI GIESCLTSNI 250
    QTSTVAELAA HPLKTFLEHG IRASINTDDP GVQGVDIIHE YTVAAPAAGL 300
    SREQIRQAQI NGLEMAFLSA EEKRALREKV AAK 333
    Length:333
    Mass (Da):36,397
    Last modified:November 1, 1997 - v2
    Checksum:i6234BBC13C505ED6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti145 – 1451Missing in AAA23419. (PubMed:1998686)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59033 Genomic DNA. Translation: AAA23419.1.
    U00096 Genomic DNA. Translation: AAC74695.1.
    AP009048 Genomic DNA. Translation: BAA15374.1.
    PIRiA64919.
    RefSeqiNP_416140.1. NC_000913.3.
    YP_489886.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74695; AAC74695; b1623.
    BAA15374; BAA15374; BAA15374.
    GeneIDi12931257.
    945851.
    KEGGiecj:Y75_p1599.
    eco:b1623.
    PATRICi32118548. VBIEscCol129921_1694.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59033 Genomic DNA. Translation: AAA23419.1 .
    U00096 Genomic DNA. Translation: AAC74695.1 .
    AP009048 Genomic DNA. Translation: BAA15374.1 .
    PIRi A64919.
    RefSeqi NP_416140.1. NC_000913.3.
    YP_489886.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P22333.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9056N.
    IntActi P22333. 15 interactions.
    MINTi MINT-1298977.
    STRINGi 511145.b1623.

    Proteomic databases

    PaxDbi P22333.
    PRIDEi P22333.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74695 ; AAC74695 ; b1623 .
    BAA15374 ; BAA15374 ; BAA15374 .
    GeneIDi 12931257.
    945851.
    KEGGi ecj:Y75_p1599.
    eco:b1623.
    PATRICi 32118548. VBIEscCol129921_1694.

    Organism-specific databases

    EchoBASEi EB0029.
    EcoGenei EG10030. add.

    Phylogenomic databases

    eggNOGi COG1816.
    HOGENOMi HOG000218815.
    KOi K01488.
    OMAi DWMVAVL.
    OrthoDBi EOG6BPDDD.
    PhylomeDBi P22333.

    Enzyme and pathway databases

    BioCyci EcoCyc:ADENODEAMIN-MONOMER.
    ECOL316407:JW1615-MONOMER.
    MetaCyc:ADENODEAMIN-MONOMER.

    Miscellaneous databases

    PROi P22333.

    Gene expression databases

    Genevestigatori P22333.

    Family and domain databases

    HAMAPi MF_00540. A_deaminase.
    InterProi IPR006650. A/AMP_deam_AS.
    IPR001365. A/AMP_deaminase_dom.
    IPR028893. A_deaminase.
    IPR006330. Ado/ade_deaminase.
    [Graphical view ]
    Pfami PF00962. A_deaminase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01430. aden_deam. 1 hit.
    PROSITEi PS00485. A_DEAMINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Deduced amino acid sequence of Escherichia coli adenosine deaminase reveals evolutionarily conserved amino acid residues: implications for catalytic function."
      Chang Z., Nygaard P., Chinault A.C., Kellems R.E.
      Biochemistry 30:2273-2280(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiADD_ECOLI
    AccessioniPrimary (citable) accession number: P22333
    Secondary accession number(s): P78163, P78240
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3