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P22333 (ADD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adenosine deaminase

EC=3.5.4.4
Alternative name(s):
Adenosine aminohydrolase
Gene names
Name:add
Ordered Locus Names:b1623, JW1615
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length333 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Adenosine + H2O = inosine + NH3. HAMAP-Rule MF_00540

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00540

Sequence similarities

Belongs to the adenosine and AMP deaminases family. Adenosine deaminase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 333333Adenosine deaminase HAMAP-Rule MF_00540
PRO_0000194367

Sites

Active site2001Proton donor By similarity
Metal binding121Zinc; catalytic By similarity
Metal binding141Zinc; catalytic By similarity
Metal binding1971Zinc; catalytic By similarity
Metal binding2781Zinc; catalytic By similarity
Binding site141Substrate By similarity
Binding site161Substrate By similarity
Binding site1701Substrate; via amide nitrogen and carbonyl oxygen By similarity
Binding site2791Substrate By similarity
Site2211Important for catalytic activity By similarity

Experimental info

Sequence conflict1451Missing in AAA23419. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P22333 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 6234BBC13C505ED6

FASTA33336,397
        10         20         30         40         50         60 
MIDTTLPLTD IHRHLDGNIR PQTILELGRQ YNISLPAQSL ETLIPHVQVI ANEPDLVSFL 

        70         80         90        100        110        120 
TKLDWGVKVL ASLDACRRVA FENIEDAARH GLHYVELRFS PGYMAMAHQL PVAGVVEAVI 

       130        140        150        160        170        180 
DGVREGCRTF GVQAKLIGIM SRTFGEAACQ QELEAFLAHR DQITALDLAG DELGFPGSLF 

       190        200        210        220        230        240 
LSHFNRARDA GWHITVHAGE AAGPESIWQA IRELGAERIG HGVKAIEDRA LMDFLAEQQI 

       250        260        270        280        290        300 
GIESCLTSNI QTSTVAELAA HPLKTFLEHG IRASINTDDP GVQGVDIIHE YTVAAPAAGL 

       310        320        330 
SREQIRQAQI NGLEMAFLSA EEKRALREKV AAK 

« Hide

References

« Hide 'large scale' references
[1]"Deduced amino acid sequence of Escherichia coli adenosine deaminase reveals evolutionarily conserved amino acid residues: implications for catalytic function."
Chang Z., Nygaard P., Chinault A.C., Kellems R.E.
Biochemistry 30:2273-2280(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map."
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. expand/collapse author list , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M59033 Genomic DNA. Translation: AAA23419.1.
U00096 Genomic DNA. Translation: AAC74695.1.
AP009048 Genomic DNA. Translation: BAA15374.1.
PIRA64919.
RefSeqNP_416140.1. NC_000913.3.
YP_489886.1. NC_007779.1.

3D structure databases

ProteinModelPortalP22333.
SMRP22333. Positions 7-326.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-9056N.
IntActP22333. 15 interactions.
MINTMINT-1298977.
STRING511145.b1623.

Proteomic databases

PaxDbP22333.
PRIDEP22333.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74695; AAC74695; b1623.
BAA15374; BAA15374; BAA15374.
GeneID12931257.
945851.
KEGGecj:Y75_p1599.
eco:b1623.
PATRIC32118548. VBIEscCol129921_1694.

Organism-specific databases

EchoBASEEB0029.
EcoGeneEG10030. add.

Phylogenomic databases

eggNOGCOG1816.
HOGENOMHOG000218815.
KOK01488.
OMADWMVAVL.
OrthoDBEOG6BPDDD.
PhylomeDBP22333.
ProtClustDBPRK09358.

Enzyme and pathway databases

BioCycEcoCyc:ADENODEAMIN-MONOMER.
ECOL316407:JW1615-MONOMER.
MetaCyc:ADENODEAMIN-MONOMER.

Gene expression databases

GenevestigatorP22333.

Family and domain databases

HAMAPMF_00540. A_deaminase.
InterProIPR006650. A/AMP_deam_AS.
IPR001365. A/AMP_deaminase_dom.
IPR028893. A_deaminase.
IPR006330. Ado/ade_deaminase.
[Graphical view]
PfamPF00962. A_deaminase. 1 hit.
[Graphical view]
TIGRFAMsTIGR01430. aden_deam. 1 hit.
PROSITEPS00485. A_DEAMINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP22333.

Entry information

Entry nameADD_ECOLI
AccessionPrimary (citable) accession number: P22333
Secondary accession number(s): P78163, P78240
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene