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Protein

Adenosine deaminase

Gene

add

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Adenosine + H2O = inosine + NH3.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12Zinc; catalyticUniRule annotation1
Metal bindingi14Zinc; catalyticUniRule annotation1
Binding sitei14SubstrateUniRule annotation1
Binding sitei16SubstrateUniRule annotation1
Binding sitei170Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi197Zinc; catalyticUniRule annotation1
Active sitei200Proton donorUniRule annotation1
Sitei221Important for catalytic activityUniRule annotation1
Metal bindingi278Zinc; catalyticUniRule annotation1
Binding sitei279SubstrateUniRule annotation1

GO - Molecular functioni

  • adenosine deaminase activity Source: EcoliWiki
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • adenosine catabolic process Source: EcoliWiki
  • cellular response to DNA damage stimulus Source: EcoliWiki
  • hypoxanthine biosynthetic process Source: EcoliWiki
  • hypoxanthine salvage Source: EcoliWiki
  • inosine biosynthetic process Source: GO_Central
  • purine nucleotide interconversion Source: EcoliWiki
  • purine nucleotide salvage Source: EcoliWiki
  • purine ribonucleoside monophosphate biosynthetic process Source: EcoliWiki

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:ADENODEAMIN-MONOMER
MetaCyc:ADENODEAMIN-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosine deaminaseUniRule annotation (EC:3.5.4.4UniRule annotation)
Alternative name(s):
Adenosine aminohydrolaseUniRule annotation
Gene namesi
Name:addUniRule annotation
Ordered Locus Names:b1623, JW1615
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10030 add

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001943671 – 333Adenosine deaminaseAdd BLAST333

Proteomic databases

EPDiP22333
PaxDbiP22333
PRIDEiP22333

Interactioni

Protein-protein interaction databases

BioGridi4260251, 30 interactors
DIPiDIP-9056N
IntActiP22333, 15 interactors
MINTiP22333
STRINGi316385.ECDH10B_1756

Structurei

3D structure databases

ProteinModelPortaliP22333
SMRiP22333
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family. Adenosine deaminase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EKD Bacteria
COG1816 LUCA
HOGENOMiHOG000218815
InParanoidiP22333
KOiK01488
OMAiQWCGADR
PhylomeDBiP22333

Family and domain databases

CDDicd01320 ADA, 1 hit
HAMAPiMF_00540 A_deaminase, 1 hit
InterProiView protein in InterPro
IPR006650 A/AMP_deam_AS
IPR001365 A/AMP_deaminase_dom
IPR028893 A_deaminase
IPR006330 Ado/ade_deaminase
IPR032466 Metal_Hydrolase
PfamiView protein in Pfam
PF00962 A_deaminase, 1 hit
SUPFAMiSSF51556 SSF51556, 1 hit
TIGRFAMsiTIGR01430 aden_deam, 1 hit
PROSITEiView protein in PROSITE
PS00485 A_DEAMINASE, 1 hit

Sequencei

Sequence statusi: Complete.

P22333-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDTTLPLTD IHRHLDGNIR PQTILELGRQ YNISLPAQSL ETLIPHVQVI
60 70 80 90 100
ANEPDLVSFL TKLDWGVKVL ASLDACRRVA FENIEDAARH GLHYVELRFS
110 120 130 140 150
PGYMAMAHQL PVAGVVEAVI DGVREGCRTF GVQAKLIGIM SRTFGEAACQ
160 170 180 190 200
QELEAFLAHR DQITALDLAG DELGFPGSLF LSHFNRARDA GWHITVHAGE
210 220 230 240 250
AAGPESIWQA IRELGAERIG HGVKAIEDRA LMDFLAEQQI GIESCLTSNI
260 270 280 290 300
QTSTVAELAA HPLKTFLEHG IRASINTDDP GVQGVDIIHE YTVAAPAAGL
310 320 330
SREQIRQAQI NGLEMAFLSA EEKRALREKV AAK
Length:333
Mass (Da):36,397
Last modified:November 1, 1997 - v2
Checksum:i6234BBC13C505ED6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti145Missing in AAA23419 (PubMed:1998686).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59033 Genomic DNA Translation: AAA23419.1
U00096 Genomic DNA Translation: AAC74695.1
AP009048 Genomic DNA Translation: BAA15374.1
PIRiA64919
RefSeqiNP_416140.1, NC_000913.3
WP_000567490.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74695; AAC74695; b1623
BAA15374; BAA15374; BAA15374
GeneIDi945851
KEGGiecj:JW1615
eco:b1623
PATRICifig|1411691.4.peg.638

Similar proteinsi

Entry informationi

Entry nameiADD_ECOLI
AccessioniPrimary (citable) accession number: P22333
Secondary accession number(s): P78163, P78240
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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