P22331 (OPSG2_ASTFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Green-sensitive opsin-2

Alternative name(s):
Green cone photoreceptor pigment 2

Gene names

Name:

G101

Organism

Astyanax fasciatus (Blind cave fish) (Astyanax mexicanus)

Taxonomic identifier

223369 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Characiphysi › Characiformes › Characidae › Characidae incertae sedis › Astyanax clade › Astyanax

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	The color pigments are found in the cone photoreceptor cells.
Miscellaneous	This fish possesses three genes for green opsin. Two (G103 and G101) that belong to the LWS/MWS group and one (RH11) that belongs to the RH2 group.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Chromophore
Molecular function	G-protein coupled receptor Photoreceptor protein Receptor Retinal protein Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological_process	phototransduction Inferred from electronic annotation. Source: UniProtKB-KW protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW photoreceptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 353	353	Green-sensitive opsin-2		PRO_0000197773
Regions
	Topological domain	1 – 47	47	Extracellular Potential
	Transmembrane	48 – 72	25	Helical; Name=1; Potential
	Topological domain	73 – 84	12	Cytoplasmic Potential
	Transmembrane	85 – 110	26	Helical; Name=2; Potential
	Topological domain	111 – 124	14	Extracellular Potential
	Transmembrane	125 – 144	20	Helical; Name=3; Potential
	Topological domain	145 – 163	19	Cytoplasmic Potential
	Transmembrane	164 – 187	24	Helical; Name=4; Potential
	Topological domain	188 – 213	26	Extracellular Potential
	Transmembrane	214 – 241	28	Helical; Name=5; Potential
	Topological domain	242 – 263	22	Cytoplasmic Potential
	Transmembrane	264 – 287	24	Helical; Name=6; Potential
	Topological domain	288 – 295	8	Extracellular Potential
	Transmembrane	296 – 320	25	Helical; Name=7; Potential
	Topological domain	321 – 353	33	Cytoplasmic Potential
Amino acid modifications
	Modified residue	307	1	N6-(retinylidene)lysine
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Disulfide bond	121 ↔ 198		Potential

Sequences

	Sequence		Length	Mass (Da)
	P22331 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: A7E7B8296AD93253	FASTA	353	39,465
	10 20 30 40 50 60 MAAHEPVFAA RRHNEDTTRE SAFVYTNANN TRDPFEGPNY HIAPRWVYNV SSLWMIFVVI 70 80 90 100 110 120 ASVFTNGLVI VATAKFKKLR HPLNWILVNL AIADLGETVL ASTISVINQI FGYFILGHPM 130 140 150 160 170 180 CVFEGWTVSV CGITALWSLT IISWERWVVV CKPFGNVKFD GKWAAGGIIF SWVWAIIWCT 190 200 210 220 230 240 PPIFGWSRYW PHGLKTSCGP DVFSGSEDPG VASYMITLML TCCILPLSII IICYIFVWSA 250 260 270 280 290 300 IHQVAQQQKD SESTQKAEKE VSRMVVVMIL AFIVCWGPYA SFATFSAVNP GYAWHPLAAA 310 320 330 340 350 MPAYFAKSAT IYNPIIYVFM NRQFRSCIMQ LFGKKVEDAS EVSGSTTEVS TAS

References

[1]	"Convergent evolution of the red- and green-like visual pigment genes in fish, Astyanax fasciatus, and human." Yokoyama R., Yokoyama S. Proc. Natl. Acad. Sci. U.S.A. 87:9315-9318(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Pineal gland.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M38624 , M38619, M38620, M38621, M38622, M38623 Genomic DNA. Translation: AAA62671.1.
PIR	A38421.
3D structure databases
ProteinModelPortal	P22331.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG107442.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR001760. Opsin. IPR000378. Opsin_red/grn. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00238. OPSIN. PR00575. OPSINREDGRN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. PS00238. OPSIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OPSG2_ASTFA

Accession

Primary (citable) accession number: P22331

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program