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Reviewed, UniProtKB/Swiss-Prot P22326 (SYY1_BACSU)

Last modified November 3, 2009. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Tyrosyl-tRNA synthetase 1
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase 1
      Short name=TyrRS 1
Gene names
Name: tyrS1
Ordered Locus Names: BSU29670
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length422 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.

Catalytic activity

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02006

Subunit structure

Homodimer. HAMAP MF_02006

Subcellular location

Cytoplasm. HAMAP MF_02006

Developmental stage

Expressed during vegetative growth. HAMAP MF_02006

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily.

Contains 1 S4 RNA-binding domain.

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Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
RNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtyrosyl-tRNA aminoacylation

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: HAMAP

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

tyrosine-tRNA ligase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 422422Tyrosyl-tRNA synthetase 1 HAMAP MF_02006
PRO_0000055643

Regions

Domain355 – 42167S4 RNA-binding
Motif40 – 4910"HIGH" region HAMAP MF_02006
Motif232 – 2365"KMSKS" region HAMAP MF_02006

Sites

Binding site351Tyrosine By similarity
Binding site1701Tyrosine By similarity
Binding site1741Tyrosine By similarity
Binding site2351ATP By similarity

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Sequences

Sequence LengthMass (Da)Tools
P22326-1 [UniParc].

Last modified August 1, 1992. Version 2.
Checksum: 141E573981303643

FASTA42247,737
        10         20         30         40         50         60 
MTNLLEDLSF RGLIQQMTDE EGLNKQLNEE KIRLYSGFDP TADSLHIGHL LPILTLRRFQ 

        70         80         90        100        110        120 
LAGHHPIALV GGATGLIGDP SGKKAERTLN TADIVSEWSQ KIKNQLSRFL DFEAAENPAV 

       130        140        150        160        170        180 
IANNFDWIGK MNVIDFLRDV GKNFGINYML AKDTVSSRIE SGISYTEFSY MILQSYDFLN 

       190        200        210        220        230        240 
LYRDKNCKLQ IGGSDQWGNI TAGLELIRKS EEEGAKAFGL TIPLVTKADG TKFGKTEGGA 

       250        260        270        280        290        300 
IWLDKEKTSP YEFYQFWINT DDRDVVKYLK YFTFLSKEEI EAYAEKTETA PEKREAQKRL 

       310        320        330        340        350        360 
AEEVTSLVHG REALEQAINI SQALFSGNIK ELSAQDVKVG FKDVPSMEVD STQELSLVDV 

       370        380        390        400        410        420 
LVQSKLSPSK RQAREDIQNG AVYINGERQT EINYTLSGED RIENQFTVLR RGKKKYFLVT 


YK

« Hide

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References

« Hide 'large scale' references
[1]"Analysis of the Bacillus subtilis tyrS gene: conservation of a regulatory sequence in multiple tRNA synthetase genes."
Henkin T.M., Glass B.L., Grundy F.J.
J. Bacteriol. 174:1299-1306(1992) [PubMed: 1735721] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region."
Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.
Microbiology 143:3431-3441(1997) [PubMed: 9387221] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"Cloning and analysis of the Bacillus subtilis rpsD gene, encoding ribosomal protein S4."
Grundy F.J., Henkin T.M.
J. Bacteriol. 172:6372-6379(1990) [PubMed: 1699930] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 328-422.
Strain: 168.

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Cross-references

Sequence databases

M77668 Genomic DNA. Translation: AAA22878.1.
M59358 Genomic DNA. Translation: AAA22718.1.
AF008220 Genomic DNA. Translation: AAC00303.1.
AL009126 Genomic DNA. Translation: CAB14945.1.
L17309 Genomic DNA. Translation: AAA68288.1.
PIRA42648.
RefSeqNP_390845.1.

3D structure databases

HSSPHSSP built from PDB template 2TS1 based on UniProtKB P00952.
SMRP22326. Positions 3-321.
ModBaseSearch...

Genome annotation databases

GeneID938185.
GenomeReviewsGene locus BSU29670 in contig AL009126_GR.
KEGGbsu:BSU29670.
NMPDRfig|224308.1.peg.2970.

Organism-specific databases

SubtiListBG10371. tyrS1. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMP22326.
OMATFYIGFD.

Enzyme and pathway databases

BioCycBSUB224308:BSU2963-MON.
BRENDA6.1.1.1. 150.

Family and domain databases

HAMAPMF_02006.
[Tree]
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ib.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA_bd.
IPR002307. Tyr-tRNA-synth_Ib_bac/mito.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHERPTHR11766. Tyr_tRNA-synt_1b. 1 hit.
PfamPF01479. S4. 1 hit.
PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01040. TRNASYNTHTYR.
SMARTSM00363. S4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00234. tyrS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameSYY1_BACSU
AccessionPrimary (citable) accession number: P22326
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1992
Last modified: November 3, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents