Reviewed, UniProtKB/Swiss-Prot P22322 (GPR_BACSU)

Names and origin

Protein names

Recommended name:
    Germination protease
    EC=3.4.24.78
Alternative name(s):
    Spore protease
    GPR endopeptidase
    Germination proteinase

Gene names

Name:	gpr
Ordered Locus Names:	BSU25540

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Initiates the degradation of small, acid-soluble proteins during spore germination. HAMAP MF_00626
Catalytic activity	Endopeptidase action with P4 Glu or Asp, P1 preferably Glu > Asp, P1' hydrophobic and P2' Ala. HAMAP MF_00626
Subunit structure	Homotetramer. HAMAP MF_00626
Developmental stage	GPR transcription occurs during sporulation in forespore first by sigma-F and then by sigma-G. HAMAP MF_00626
Post-translational modification	Autoproteolytically processed. The inactive tetrameric zymogen termed p46 autoprocesses to a smaller form termed p41, which is active only during spore germination. HAMAP MF_00626
Sequence similarities	Belongs to the peptidase A25 family.

Ontologies

Keywords
Molecular function	Hydrolase Protease
PTM	Zymogen
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: HAMAP spore germination Inferred from electronic annotation. Source: HAMAP
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 16	16	HAMAP MF_00626		PRO_0000026870
	Chain	17 – 368	352	Germination protease HAMAP MF_00626		PRO_0000026871

Sequences

	Sequence		Length	Mass (Da)
	P22322-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 7F69F6739199CA43	FASTA	368	40,283
	10 20 30 40 50 60 MKKSELDVNQ YLIRTDLAVE TKEAMANQQA VPTKEIKGFI EKERDHGGIK IRTVDVTKEG 70 80 90 100 110 120 AELSGKKEGR YLTLEAQGIR ENDSEMQEKV SAVFAEEFSA FLENLNISKD ASCLIVGLGN 130 140 150 160 170 180 WNVTPDALGP MAVENLLVTR HLFKLQPENV QEGYRPVSAF APGVMGITGI ETSDIIKGVI 190 200 210 220 230 240 EQSKPDFVIA IDALAARAVE RVNTTIQISD TGIHPGSGVG NKRKDLSKDT LGVPVIAIGV 250 260 270 280 290 300 PTVVDAVTIA SDTVDYILKH FGREMKDNRP SRSLVPAGMT FGKKKVLTED DLPDQKQRQS 310 320 330 340 350 360 FLGIVGTLQE DEKRQLIHEV LSPLGHNLMV TPKEVDSFID DMANVLANGL NTALHEKVSQ ENKGSYNH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, nucleotide sequence, and regulation of the Bacillus subtilis gpr gene, which codes for the protease that initiates degradation of small, acid-soluble proteins during spore germination." Sussman M.D., Setlow P. J. Bacteriol. 173:291-300(1991) [PubMed: 1840582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes." Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y. Microbiology 142:3103-3111(1996) [PubMed: 8969508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / JH642.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Proteolytic processing of the protease which initiates degradation of small, acid-soluble proteins during germination of Bacillus subtilis spores." Sanchez-Salas J.-L., Setlow P. J. Bacteriol. 175:2568-2577(1993) [PubMed: 8478323] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-6 AND 17-23.
[5]	Takemaru K., Sato T., Kobayashi Y. Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 144-368. Strain: 168 / JH642.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M55263 Genomic DNA. Translation: AAA22500.1. D84432 Genomic DNA. Translation: BAA12457.1. AL009126 Genomic DNA. Translation: CAB14496.1. D17650 Genomic DNA. Translation: BAA04541.1.
PIR	B39198.
RefSeq	NP_390432.1.
3D structure databases
HSSP	HSSP built from PDB template 1C8B based on UniProtKB P22321.
ModBase	Search...
Protein family/group databases
MEROPS	A25.001.
Genome annotation databases
GeneID	937838.
GenomeReviews	Gene locus BSU25540 in contig AL009126_GR.
KEGG	bsu:BSU25540.
NMPDR	fig|224308.1.peg.2557.
Organism-specific databases
SubtiList	BG10438. gpr. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P22322.
OMA	P22322. LMVTRHL.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU2550-MON.
BRENDA	3.4.24.78. 150.
Family and domain databases
HAMAP	MF_00626. [Tree]
InterPro	IPR005080. Peptidase_A25. [Graphical view]
Gene3D	G3DSA:3.40.50.10100. Peptidase_A25. 1 hit.
Pfam	PF03418. Peptidase_A25. 1 hit. [Graphical view]
PIRSF	PIRSF019549. Peptidase_A25. 1 hit.
ProDom	PD041835. Peptidase_A25. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01441. GPR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GPR_BACSU

Accession

Primary (citable) accession number: P22322

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	June 16, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)