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Protein

Germination protease

Gene

gpr

Organism
Bacillus megaterium (strain ATCC 12872 / QMB1551)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Initiates the rapid degradation of small, acid-soluble proteins during spore germination.

Catalytic activityi

Endopeptidase action with P4 Glu or Asp, P1 preferably Glu > Asp, P1' hydrophobic and P2' Ala.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Enzyme and pathway databases

BRENDAi3.4.24.78. 656.

Protein family/group databases

MEROPSiA25.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Germination protease (EC:3.4.24.78)
Alternative name(s):
GPR endopeptidase
Germination proteinase
Spore protease
Gene namesi
Name:gpr
Ordered Locus Names:BMQ_4571
OrganismiBacillus megaterium (strain ATCC 12872 / QMB1551)
Taxonomic identifieri545693 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000000935 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000268681 – 151 PublicationAdd BLAST15
ChainiPRO_000002686916 – 370Germination proteaseAdd BLAST355

Post-translational modificationi

Autoproteolytically processed. The inactive tetrameric zymogen termed p46 autoprocesses to a smaller form termed p41, which is active only during spore germination.

Keywords - PTMi

Zymogen

Expressioni

Developmental stagei

GPR transcription occurs during sporulation in forespore first by sigma-F and then by sigma-G.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi545693.BMQ_4571.

Structurei

Secondary structure

1370
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi19 – 27Combined sources9
Beta strandi43 – 51Combined sources9
Beta strandi54 – 61Combined sources8
Helixi63 – 66Combined sources4
Turni67 – 70Combined sources4
Beta strandi74 – 80Combined sources7
Beta strandi84 – 89Combined sources6
Helixi90 – 105Combined sources16
Beta strandi114 – 118Combined sources5
Beta strandi122 – 124Combined sources3
Helixi125 – 127Combined sources3
Helixi129 – 135Combined sources7
Helixi141 – 146Combined sources6
Beta strandi158 – 161Combined sources4
Helixi164 – 166Combined sources3
Helixi173 – 184Combined sources12
Beta strandi187 – 195Combined sources9
Helixi200 – 202Combined sources3
Beta strandi203 – 210Combined sources8
Beta strandi218 – 221Combined sources4
Beta strandi226 – 229Combined sources4
Beta strandi245 – 251Combined sources7
Beta strandi254 – 256Combined sources3
Helixi260 – 263Combined sources4
Turni264 – 268Combined sources5
Helixi315 – 322Combined sources8
Beta strandi330 – 334Combined sources5
Helixi337 – 352Combined sources16
Helixi353 – 356Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C8BX-ray3.00A/B1-370[»]
SMRiP22321.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22321.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A25 family.Curated

Phylogenomic databases

HOGENOMiHOG000058956.
KOiK06012.
OMAiPMGNYIT.
OrthoDBiPOG091H0WE2.

Family and domain databases

Gene3Di3.40.50.1450. 2 hits.
HAMAPiMF_00626. Germination_prot. 1 hit.
InterProiIPR023430. Pept_HybD-like_dom.
IPR005080. Peptidase_A25.
[Graphical view]
PfamiPF03418. Peptidase_A25. 1 hit.
[Graphical view]
PIRSFiPIRSF019549. Peptidase_A25. 1 hit.
ProDomiPD041835. Peptidase_A25. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53163. SSF53163. 1 hit.
TIGRFAMsiTIGR01441. GPR. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22321-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKELDLSQY SVRTDLAVEA KDIALENQPK PNNQSEIKGV IVKEKEEQGV
60 70 80 90 100
KISMVEITEE GAEAIGKKKG RYVTLESVGI REQDTEKQEA MEEVFAKELN
110 120 130 140 150
FFIKSLNIPD DASCLVVGLG NLSVTPDALG PKAVDNLLIT RHLFELQPES
160 170 180 190 200
VQDGFRPVSA IVPGVMGMTG IETSDIIFGV VKKVNPDFII AIDALAARSI
210 220 230 240 250
ERVNATIQIS DSGIHPGSGV GNKRKEISYE TLGIPVIAIG IPTVVDAVSI
260 270 280 290 300
TSDTIDFILK HFGREMKEQG KPSKSLLPSG MTFGEKKKLT EDDLPNEEQR
310 320 330 340 350
QTYLGMIGTL PDEEKRRLIH EVLAPLGHNL MVTPKEVDMF IEDMANVVAG
360 370
GLNAALHHEV DQENFGAYTH
Length:370
Mass (Da):40,497
Last modified:August 10, 2010 - v2
Checksum:iF235C361792C37EC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti89E → EE in AAA22499 (PubMed:1840582).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55262 Genomic DNA. Translation: AAA22499.1.
CP001983 Genomic DNA. Translation: ADE71575.1.
PIRiA39198.
RefSeqiWP_013059248.1. NC_014019.1.

Genome annotation databases

EnsemblBacteriaiADE71575; ADE71575; BMQ_4571.
KEGGibmq:BMQ_4571.
PATRICi35485914. VBIBacMeg35839_4612.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55262 Genomic DNA. Translation: AAA22499.1.
CP001983 Genomic DNA. Translation: ADE71575.1.
PIRiA39198.
RefSeqiWP_013059248.1. NC_014019.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C8BX-ray3.00A/B1-370[»]
SMRiP22321.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi545693.BMQ_4571.

Protein family/group databases

MEROPSiA25.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADE71575; ADE71575; BMQ_4571.
KEGGibmq:BMQ_4571.
PATRICi35485914. VBIBacMeg35839_4612.

Phylogenomic databases

HOGENOMiHOG000058956.
KOiK06012.
OMAiPMGNYIT.
OrthoDBiPOG091H0WE2.

Enzyme and pathway databases

BRENDAi3.4.24.78. 656.

Miscellaneous databases

EvolutionaryTraceiP22321.

Family and domain databases

Gene3Di3.40.50.1450. 2 hits.
HAMAPiMF_00626. Germination_prot. 1 hit.
InterProiIPR023430. Pept_HybD-like_dom.
IPR005080. Peptidase_A25.
[Graphical view]
PfamiPF03418. Peptidase_A25. 1 hit.
[Graphical view]
PIRSFiPIRSF019549. Peptidase_A25. 1 hit.
ProDomiPD041835. Peptidase_A25. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53163. SSF53163. 1 hit.
TIGRFAMsiTIGR01441. GPR. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGPR_BACMQ
AccessioniPrimary (citable) accession number: P22321
Secondary accession number(s): D5DSW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 10, 2010
Last modified: November 30, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.