P22321 (GPR_BACMQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Germination protease
EC=3.4.24.78

Alternative name(s):
GPR endopeptidase
Germination proteinase
Spore protease

Gene names

Name:	gpr
Ordered Locus Names:	BMQ_4571

Organism

Bacillus megaterium (strain ATCC 12872 / QMB1551) [Complete proteome] [HAMAP]

Taxonomic identifier

545693 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Initiates the rapid degradation of small, acid-soluble proteins during spore germination. HAMAP MF_00626
Catalytic activity	Endopeptidase action with P4 Glu or Asp, P1 preferably Glu > Asp, P1' hydrophobic and P2' Ala. HAMAP MF_00626
Subunit structure	Homotetramer.
Developmental stage	GPR transcription occurs during sporulation in forespore first by sigma-F and then by sigma-G. HAMAP MF_00626
Post-translational modification	Autoproteolytically processed. The inactive tetrameric zymogen termed p46 autoprocesses to a smaller form termed p41, which is active only during spore germination. HAMAP MF_00626
Sequence similarities	Belongs to the peptidase A25 family.

Ontologies

Keywords
Molecular function	Hydrolase Protease
PTM	Zymogen
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW spore germination Inferred from electronic annotation. Source: InterPro
Molecular function	peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Propeptide

1 – 15

15

HAMAP MF_00626

PRO_0000026868

Chain

16 – 370

355

Germination protease HAMAP MF_00626

PRO_0000026869

Experimental info

Sequence conflict

89

1

E → EE in AAA22499. Ref.1

Secondary structure

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370

Helix Strand Turn

Details...

Helix

19 – 27

9

Beta strand

43 – 51

9

Beta strand

54 – 61

8

Helix

63 – 66

4

Turn

67 – 70

4

Beta strand

74 – 80

7

Beta strand

84 – 89

6

Helix

90 – 105

16

Beta strand

114 – 118

5

Beta strand

122 – 124

3

Helix

125 – 127

3

Helix

129 – 135

7

Helix

141 – 146

6

Beta strand

158 – 161

4

Helix

164 – 166

3

Helix

173 – 184

12

Beta strand

187 – 195

9

Helix

200 – 202

3

Beta strand

203 – 210

8

Beta strand

218 – 221

4

Beta strand

226 – 229

4

Beta strand

245 – 251

7

Beta strand

254 – 256

3

Helix

260 – 263

4

Turn

264 – 268

5

Helix

315 – 322

8

Beta strand

330 – 334

5

Helix

337 – 352

16

Helix

353 – 356

4

Sequences

	Sequence		Length	Mass (Da)
	P22321 [UniParc]. Last modified August 10, 2010. Version 2. Checksum: F235C361792C37EC	FASTA	370	40,497
	10 20 30 40 50 60 MEKELDLSQY SVRTDLAVEA KDIALENQPK PNNQSEIKGV IVKEKEEQGV KISMVEITEE 70 80 90 100 110 120 GAEAIGKKKG RYVTLESVGI REQDTEKQEA MEEVFAKELN FFIKSLNIPD DASCLVVGLG 130 140 150 160 170 180 NLSVTPDALG PKAVDNLLIT RHLFELQPES VQDGFRPVSA IVPGVMGMTG IETSDIIFGV 190 200 210 220 230 240 VKKVNPDFII AIDALAARSI ERVNATIQIS DSGIHPGSGV GNKRKEISYE TLGIPVIAIG 250 260 270 280 290 300 IPTVVDAVSI TSDTIDFILK HFGREMKEQG KPSKSLLPSG MTFGEKKKLT EDDLPNEEQR 310 320 330 340 350 360 QTYLGMIGTL PDEEKRRLIH EVLAPLGHNL MVTPKEVDMF IEDMANVVAG GLNAALHHEV 370 DQENFGAYTH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, nucleotide sequence, and regulation of the Bacillus subtilis gpr gene, which codes for the protease that initiates degradation of small, acid-soluble proteins during spore germination." Sussman M.D., Setlow P. J. Bacteriol. 173:291-300(1991) [PubMed: 1840582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 16-31.
[2]	"Genome sequences of the industrial vitamin B12-producers B. megaterium QM B1551 and DSM319 reveal new insights into the Bacillus genome evolution and pan-genome structure." Eppinger M., Bunk B., Johns M.A., Edirisinghe J.N., Kutumbaka K.K., Riley D.R., Creasy H.H., Koenig S.S.K., Galens K., Orvis J., Creasy T., Biedendieck R., Braun C., Grayburn S., Jahn D., Ravel J., Vary P.S. Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 12872 / QMB1551.
[3]	"Structure and mechanism of action of the protease that degrades small, acid-soluble spore proteins during germination of spores of Bacillus species." Nessi C., Jedrzejas M.J., Setlow P. J. Bacteriol. 180:5077-5084(1998) [PubMed: 9748439] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Crystal structure of a novel germination protease from spores of Bacillus megaterium: structural arrangement and zymogen activation." Ponnuraj K., Rowland S., Nessi C., Setlow P., Jedrzejas M.J. J. Mol. Biol. 300:1-10(2000) [PubMed: 10864493] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF ZYMOGEN P46.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M55262 Genomic DNA. Translation: AAA22499.1.
CP001983 Genomic DNA. Translation: ADE71575.1.

PIR

A39198.

RefSeq

YP_003565009.1. NC_014019.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1C8B	X-ray	3.00	A/B	1-370	[»]

ModBase

Search...

Protein family/group databases

MEROPS

A25.001.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBBACT00000391700; EBBACP00000384501; EBBACG00000392381.

GeneID

8989009.

GenomeReviews

Gene locus BMQ_4571 in contig CP001983_GR.

KEGG

bmq:BMQ_4571.

PATRIC

35485914. VBIBacMeg35839_4612.

Organism-specific databases

CMR

Search...

Phylogenomic databases

GeneTree

EBGT00050000001716.

ProtClustDB

PRK02858.

Family and domain databases

HAMAP

MF_00626. Germination_prot.
[Tree]

InterPro

IPR023430. Pept_HybD-like_dom.
IPR005080. Peptidase_A25.
[Graphical view]

Gene3D

G3DSA:3.40.50.1450. Peptidase_A31. 2 hits.

KO

K06012.

Pfam

PF03418. Peptidase_A25. 1 hit.
[Graphical view]

PIRSF

PIRSF019549. Peptidase_A25. 1 hit.

SUPFAM

SSF53163. SSF53163. 1 hit.

TIGRFAMs

TIGR01441. GPR. 1 hit.

ProtoNet

Search...

Entry information

Entry name

GPR_BACMQ

Accession

Primary (citable) accession number: P22321
Secondary accession number(s): D5DSW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 10, 2010
Last modified:	January 25, 2012
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program