Reviewed, UniProtKB/Swiss-Prot P22319 (HOXY_RALEH)

Names and origin

Protein names

Recommended name:
    NAD-reducing hydrogenase hoxS subunit delta
    EC=1.12.1.2

Gene names

Name:	hoxY
Ordered Locus Names:	PHG090

Encoded on

Plasmid megaplasmid pHG1

Organism

Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	H2 + NAD+ = H+ + NADH.
Cofactor	Binds 2 4Fe-4S clusters. Binds 1 3Fe-4S cluster. Binds 1 2Fe-2S cluster. FMN. Nickel.
Subunit structure	Tetramer of an alpha and a gamma subunits (flavin-containing dimer), and a delta and a nickel-containing beta subunits (hydrogenase dimer).
Subcellular location	Cytoplasm.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro hydrogen dehydrogenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 209	209	NAD-reducing hydrogenase hoxS subunit delta		PRO_0000199734

Sequences

	Sequence		Length	Mass (Da)
	P22319-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 247505ED38604686	FASTA	209	22,881
	10 20 30 40 50 60 MRAPHKDEIA SHELPATPMD PALAANREGK IKVATIGLCG CWGCTLSFLD MDERLLPLLE 70 80 90 100 110 120 KVTLLRSSLT DIKRIPERCA IGFVEGGVSS EENIETLEHF RENCDILISV GACAVWGGVP 130 140 150 160 170 180 AMRNVFELKD CLAEAYVNSA TAVPGAKAVV PFHPDIPRIT TKVYPCHEVV KMDYFIPGCP 190 200 PDGDAIFKVL DDLVNGRPFD LPSSINRYD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and nucleotide sequences of the genes for the subunits of NAD-reducing hydrogenase of Alcaligenes eutrophus H16." Tran-Betcke A., Warnecke U., Boecker C., Zaborosch C., Friedrich B. J. Bacteriol. 172:2920-2929(1990) [PubMed: 2188945] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of pHG1: a Ralstonia eutropha H16 megaplasmid encoding key enzymes of H(2)-based lithoautotrophy and anaerobiosis." Schwartz E., Henne A., Cramm R., Eitinger T., Friedrich B., Gottschalk G. J. Mol. Biol. 332:369-383(2003) [PubMed: 12948488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Comparison of the NH2-terminal amino acid sequences of the four non-identical subunits of the NAD-linked hydrogenases from Nocardia opaca 1b and Alcaligenes eutrophus H16." Zaborosch C., Schneider K., Schlegel H.G., Kratzin H. Eur. J. Biochem. 181:175-180(1989) [PubMed: 2496982] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-20.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M55230 Genomic DNA. Translation: AAC06142.1. AY305378 Genomic DNA. Translation: AAP85843.1.
PIR	C35385.
RefSeq	NP_942729.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2656816.
GenomeReviews	Gene locus PHG090 in contig AY305378_GR.
KEGG	reh:PHG090.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P22319.
OMA	P22319. ERCAIGF.
Enzyme and pathway databases
BioCyc	MetaCyc:HOXYALCA-MON.
BRENDA	1.12.1.2. 275933.
Family and domain databases
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. [Graphical view]
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOXY_RALEH

Accession

Primary (citable) accession number: P22319

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)