Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NAD-reducing hydrogenase HoxS subunit gamma

Gene

hoxU

Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Subunits alpha and gamma of HoxS constitute an NADH--oxidoreductase.

Catalytic activityi

H2 + NAD+ = H+ + NADH.

Cofactori

[4Fe-4S] clusterCuratedNote: Binds 3 [4Fe-4S] clusters per subunit.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi35Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi46Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi49Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi61Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi95Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi97Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi100Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi106Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi145Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi148Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi151Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi198Iron-sulfur 3 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Ligand2Fe-2S, 4Fe-4S, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyciMetaCyc:HOXUALCA-MONOMER
BRENDAi1.12.1.2 231

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-reducing hydrogenase HoxS subunit gamma (EC:1.12.1.2)
Gene namesi
Name:hoxU
Ordered Locus Names:PHG089
Encoded oniPlasmid megaplasmid pHG10 Publication
OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Taxonomic identifieri381666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi
  • UP000008210 Componenti: Plasmid megaplasmid pHG1

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001185382 – 234NAD-reducing hydrogenase HoxS subunit gammaAdd BLAST233

Interactioni

Subunit structurei

Tetramer of an alpha and a gamma subunits (flavin-containing dimer), and a delta and a nickel-containing beta subunits (hydrogenase dimer).

Structurei

3D structure databases

ProteinModelPortaliP22318
SMRiP22318
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 772Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST76
Domaini77 – 1164Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST40

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated

Phylogenomic databases

HOGENOMiHOG000009978
KOiK18006
OMAiNHVCAVC

Family and domain databases

CDDicd00207 fer2, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR016214 NAD-red_Hydgase_HoxS_gsu
IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS
IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd
PfamiView protein in Pfam
PF10588 NADH-G_4Fe-4S_3, 1 hit
PIRSFiPIRSF000309 NAD_red_hyd_HoxU, 1 hit
SMARTiView protein in SMART
SM00929 NADH-G_4Fe-4S_3, 1 hit
SUPFAMiSSF54292 SSF54292, 1 hit
PROSITEiView protein in PROSITE
PS51085 2FE2S_FER_2, 1 hit
PS51839 4FE4S_HC3, 1 hit
PS00641 COMPLEX1_75K_1, 1 hit
PS00642 COMPLEX1_75K_2, 1 hit
PS00643 COMPLEX1_75K_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22318-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIQITIDGK TLTTEEGRTL VDVAAENGVY IPTLCYLKDK PCLGTCRVCS
60 70 80 90 100
VKVNGNVAAA CTVRVSKGLN VEVNDPELVD MRKALVEFLF AEGNHNCPSC
110 120 130 140 150
EKSGRCQLQA VGYEVDMMVS RFPYRFPVRV VDHASEKIWL ERDRCIFCQR
160 170 180 190 200
CVEFIRDKAS GRKIFSISHR GPESRIEIDA ELANAMPPEQ VKEAVAICPV
210 220 230
GTILEKRVGY DDPIGRRKYE IQSVRARALE GEDK
Length:234
Mass (Da):26,173
Last modified:January 23, 2007 - v2
Checksum:i38E59021F7B82A2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55230 Genomic DNA Translation: AAC06141.1
AY305378 Genomic DNA Translation: AAP85842.1
PIRiB35385
RefSeqiWP_011154011.1, NC_005241.1

Genome annotation databases

EnsemblBacteriaiAAP85842; AAP85842; PHG089
KEGGireh:PHG089
PATRICifig|381666.6.peg.66

Similar proteinsi

Entry informationi

Entry nameiHOXU_CUPNH
AccessioniPrimary (citable) accession number: P22318
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Plasmid

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health