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P22317 (HOXF_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD-reducing hydrogenase hoxS subunit alpha
EC=1.12.1.2
Gene names
Name:hoxF
Ordered Locus Names:PHG088
Encoded onPlasmid megaplasmid pHG1
OrganismCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]
Taxonomic identifier381666 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length602 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Subunits alpha and gamma of hoxS constitute an NADH--oxidoreductase.

Catalytic activity

H2 + NAD+ = H+ + NADH.

Cofactor

Binds 1 FMN Potential.

Binds 1 4Fe-4S cluster Potential.

Subunit structure

Tetramer of an alpha and a gamma subunits (flavin-containing dimer), and a delta and a nickel-containing beta subunit (hydrogenase dimer).

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the complex I 51 kDa subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 602602NAD-reducing hydrogenase hoxS subunit alpha
PRO_0000118564

Regions

Nucleotide binding219 – 22810NAD By similarity
Nucleotide binding332 – 37948FMN By similarity

Sites

Metal binding4991Iron-sulfur (4Fe-4S) Potential
Metal binding5021Iron-sulfur (4Fe-4S) Potential
Metal binding5051Iron-sulfur (4Fe-4S) Potential
Metal binding5451Iron-sulfur (4Fe-4S) Potential

Experimental info

Sequence conflict241W → G AA sequence Ref.3

Sequences

Sequence LengthMass (Da)Tools
P22317 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: B7D9BBB11AB53F6C

FASTA60266,784
        10         20         30         40         50         60 
MDSRITTILE RYRSDRTRLI DILWDVQHEY GHIPDAVLPQ LGAGLKLSPL DIRETASFYH 

        70         80         90        100        110        120 
FFLDKPSGKY RIYLCNSVIA KINGYQAVRE ALERETGIRF GETDPNGMFG LFDTPCIGLS 

       130        140        150        160        170        180 
DQEPAMLIDK VVFTRLRPGK ITDIIAQLKQ GRSPAEIANP AGLPSQDIAY VDAMVESNVR 

       190        200        210        220        230        240 
TKGPVFFRGR TDLRSLLDQC LLLKPEQVIE TIVDSRLRGR GGAGFSTGLK WRLCRDAESE 

       250        260        270        280        290        300 
QKYVICNADE GEPGTFKDRV LLTRAPKKVF VGMVIAAYAI GCRKGIVYLR GEYFYLKDYL 

       310        320        330        340        350        360 
ERQLQELRED GLLGRAIGGR AGFDFDIRIQ MGAGAYICGD ESALIESCEG KRGTPRVKPP 

       370        380        390        400        410        420 
FPVQQGYLGK PTSVNNVETF AAVSRIMEEG ADWFRAMGTP DSAGTRLLSV AGDCSKPGIY 

       430        440        450        460        470        480 
EVEWGVTLNE VLAMVGARDA RAVQISGPSG ECVSVAKDGE RKLAYEDLSC NGAFTIFNCK 

       490        500        510        520        530        540 
RDLLEIVRDH MQFFVEESCG ICVPCRAGNV DLHRKVEWVI AGKACQKDLD DMVSWGALVR 

       550        560        570        580        590        600 
RTSRCGLGAT SPKPILTTLE KFPEIYQNKL VRHEGPLLPS FDLDTALGGY EKALKDLEEV 


TR

« Hide

References

« Hide 'large scale' references
[1]"Cloning and nucleotide sequences of the genes for the subunits of NAD-reducing hydrogenase of Alcaligenes eutrophus H16."
Tran-Betcke A., Warnecke U., Boecker C., Zaborosch C., Friedrich B.
J. Bacteriol. 172:2920-2929(1990) [PubMed: 2188945] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete nucleotide sequence of pHG1: a Ralstonia eutropha H16 megaplasmid encoding key enzymes of H(2)-based lithoautotrophy and anaerobiosis."
Schwartz E., Henne A., Cramm R., Eitinger T., Friedrich B., Gottschalk G.
J. Mol. Biol. 332:369-383(2003) [PubMed: 12948488] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.
[3]"Comparison of the NH2-terminal amino acid sequences of the four non-identical subunits of the NAD-linked hydrogenases from Nocardia opaca 1b and Alcaligenes eutrophus H16."
Zaborosch C., Schneider K., Schlegel H.G., Kratzin H.
Eur. J. Biochem. 181:175-180(1989) [PubMed: 2496982] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-27.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M55230 Genomic DNA. Translation: AAC06140.1.
AY305378 Genomic DNA. Translation: AAP85841.1.
PIRA35385.
RefSeqNP_942727.1. NC_005241.1.

3D structure databases

ProteinModelPortalP22317.
ModBaseSearch...

Protein-protein interaction databases

STRINGP22317.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2656814.
GenomeReviewsGene locus PHG088 in contig AY305378_GR.
KEGGreh:PHG088.
PATRIC35228786. VBIRalEut6770_0065.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1894.
HOGENOMHBG669338.
OMAFCTPCRV.
PhylomeDBP22317.
ProtClustDBCLSK923807.

Enzyme and pathway databases

BioCycMetaCyc:HOXFALCA-MONOMER.
REUT381666:PHG088-MONOMER.
BRENDA1.12.1.2. 7290.

Family and domain databases

InterProIPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd.
IPR002023. NADH_UbQ_OxRdtase_24kDa_su.
IPR011538. NADH_UbQ_OxRdtase_51kDa_su.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
Gene3DG3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
KOK00436.
PfamPF01257. Complex1_24kDa. 1 hit.
PF01512. Complex1_51K. 1 hit.
PF10589. NADH_4Fe-4S. 1 hit.
[Graphical view]
SMARTSM00928. NADH_4Fe-4S. 1 hit.
[Graphical view]
SUPFAMSSF52833. Thiordxn-like_fd. 1 hit.
PROSITEPS00644. COMPLEX1_51K_1. 1 hit.
PS00645. COMPLEX1_51K_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHOXF_CUPNH
AccessionPrimary (citable) accession number: P22317
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: January 25, 2012
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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