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Protein

Interleukin-10

Gene

IL10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

GO - Molecular functioni

  • cytokine activity Source: BHF-UCL
  • growth factor activity Source: UniProtKB
  • interleukin-10 receptor binding Source: UniProtKB

GO - Biological processi

  • aging Source: Ensembl
  • B cell differentiation Source: UniProtKB
  • B cell proliferation Source: UniProtKB
  • branching involved in labyrinthine layer morphogenesis Source: Ensembl
  • cell-cell signaling Source: UniProtKB
  • cellular response to estradiol stimulus Source: Ensembl
  • cellular response to hepatocyte growth factor stimulus Source: Ensembl
  • cellular response to lipopolysaccharide Source: BHF-UCL
  • cytoplasmic sequestering of NF-kappaB Source: UniProtKB
  • defense response to bacterium Source: Ensembl
  • defense response to protozoan Source: Ensembl
  • endothelial cell apoptotic process Source: BHF-UCL
  • hemopoiesis Source: UniProtKB
  • inflammatory response Source: BHF-UCL
  • leukocyte chemotaxis Source: ProtInc
  • liver regeneration Source: Ensembl
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of B cell proliferation Source: MGI
  • negative regulation of cell proliferation Source: BHF-UCL
  • negative regulation of chemokine (C-C motif) ligand 5 production Source: AgBase
  • negative regulation of chronic inflammatory response to antigenic stimulus Source: Ensembl
  • negative regulation of cytokine activity Source: CACAO
  • negative regulation of cytokine secretion involved in immune response Source: BHF-UCL
  • negative regulation of growth of symbiont in host Source: Ensembl
  • negative regulation of heterotypic cell-cell adhesion Source: BHF-UCL
  • negative regulation of interferon-alpha biosynthetic process Source: UniProtKB
  • negative regulation of interferon-gamma production Source: Ensembl
  • negative regulation of interleukin-12 production Source: AgBase
  • negative regulation of interleukin-18 production Source: AgBase
  • negative regulation of interleukin-1 production Source: AgBase
  • negative regulation of interleukin-6 production Source: BHF-UCL
  • negative regulation of interleukin-8 production Source: AgBase
  • negative regulation of membrane protein ectodomain proteolysis Source: BHF-UCL
  • negative regulation of MHC class II biosynthetic process Source: UniProtKB
  • negative regulation of mitotic cell cycle Source: BHF-UCL
  • negative regulation of myeloid dendritic cell activation Source: Ensembl
  • negative regulation of nitric oxide biosynthetic process Source: Ensembl
  • negative regulation of sensory perception of pain Source: Ensembl
  • negative regulation of T cell proliferation Source: UniProtKB
  • negative regulation of tumor necrosis factor biosynthetic process Source: Ensembl
  • negative regulation of tumor necrosis factor production Source: AgBase
  • negative regulation of vascular smooth muscle cell proliferation Source: BHF-UCL
  • positive regulation of B cell apoptotic process Source: MGI
  • positive regulation of cytokine secretion Source: BHF-UCL
  • positive regulation of heterotypic cell-cell adhesion Source: BHF-UCL
  • positive regulation of JAK-STAT cascade Source: GO_Central
  • positive regulation of macrophage activation Source: Ensembl
  • positive regulation of MHC class II biosynthetic process Source: Ensembl
  • positive regulation of sequence-specific DNA binding transcription factor activity Source: BHF-UCL
  • positive regulation of transcription, DNA-templated Source: BHF-UCL
  • positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  • positive regulation of vascular smooth muscle cell proliferation Source: BHF-UCL
  • receptor biosynthetic process Source: BHF-UCL
  • regulation of gene expression Source: MGI
  • regulation of isotype switching Source: UniProtKB
  • regulation of response to wounding Source: BHF-UCL
  • regulation of synapse organization Source: Ensembl
  • response to activity Source: Ensembl
  • response to carbon monoxide Source: Ensembl
  • response to drug Source: Ensembl
  • response to glucocorticoid Source: BHF-UCL
  • response to inactivity Source: Ensembl
  • response to insulin Source: Ensembl
  • response to molecule of bacterial origin Source: UniProtKB
  • type 2 immune response Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Enzyme and pathway databases

BioCyciZFISH:ENSG00000136634-MONOMER.
SignaLinkiP22301.
SIGNORiP22301.

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-10
Short name:
IL-10
Alternative name(s):
Cytokine synthesis inhibitory factor
Short name:
CSIF
Gene namesi
Name:IL10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:5962. IL10.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GOC
  • extracellular space Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Keywords - Diseasei

Disease mutation

Organism-specific databases

DisGeNETi3586.
MalaCardsiIL10.
MIMi614395. phenotype.
OpenTargetsiENSG00000136634.
Orphaneti238569. Autosomal recessive early-onset inflammatory bowel disease.
117. Behcet disease.
536. Systemic lupus erythematosus.
PharmGKBiPA29778.

Polymorphism and mutation databases

BioMutaiIL10.
DMDMi124292.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 181 PublicationAdd BLAST18
ChainiPRO_000001536019 – 178Interleukin-10Add BLAST160

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi30 ↔ 1261 Publication
Disulfide bondi80 ↔ 1321 Publication
Glycosylationi134N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP22301.
PeptideAtlasiP22301.
PRIDEiP22301.

Expressioni

Tissue specificityi

Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Gene expression databases

BgeeiENSG00000136634.
CleanExiHS_IL10.
ExpressionAtlasiP22301. baseline and differential.
GenevisibleiP22301. HS.

Organism-specific databases

HPAiCAB013120.

Interactioni

Subunit structurei

Homodimer.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
IL10RAQ136516EBI-1031632,EBI-1031656

GO - Molecular functioni

  • cytokine activity Source: BHF-UCL
  • growth factor activity Source: UniProtKB
  • interleukin-10 receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi109800. 3 interactors.
DIPiDIP-3511N.
IntActiP22301. 7 interactors.
MINTiMINT-206740.
STRINGi9606.ENSP00000412237.

Structurei

Secondary structure

1178
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni33 – 36Combined sources4
Helixi37 – 49Combined sources13
Helixi52 – 58Combined sources7
Helixi68 – 75Combined sources8
Turni76 – 78Combined sources3
Helixi79 – 93Combined sources15
Helixi95 – 99Combined sources5
Turni103 – 105Combined sources3
Helixi106 – 125Combined sources20
Turni127 – 129Combined sources3
Helixi131 – 133Combined sources3
Helixi137 – 148Combined sources12
Helixi151 – 159Combined sources9
Helixi161 – 176Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ILKX-ray1.80A28-178[»]
1INRX-ray2.00A19-178[»]
1J7VX-ray2.90L19-178[»]
1LK3X-ray1.91A/B26-178[»]
1Y6KX-ray2.52L19-178[»]
2H24X-ray2.00A19-178[»]
2ILKX-ray1.60A19-178[»]
ProteinModelPortaliP22301.
SMRiP22301.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22301.

Family & Domainsi

Sequence similaritiesi

Belongs to the IL-10 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IYFW. Eukaryota.
ENOG4111PXC. LUCA.
GeneTreeiENSGT00510000048535.
HOGENOMiHOG000072692.
HOVERGENiHBG004110.
InParanoidiP22301.
KOiK05443.
OMAiPHMLREL.
OrthoDBiEOG091G0Y3M.
PhylomeDBiP22301.
TreeFamiTF333253.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR000098. IL-10.
IPR020443. IL-10/19/20/22/24/26_fam.
IPR020423. IL-10_CS.
[Graphical view]
PANTHERiPTHR11585. PTHR11585. 1 hit.
PfamiPF00726. IL10. 1 hit.
[Graphical view]
PRINTSiPR01294. INTRLEUKIN10.
SMARTiSM00188. IL10. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00520. INTERLEUKIN_10. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22301-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHSSALLCCL VLLTGVRASP GQGTQSENSC THFPGNLPNM LRDLRDAFSR
60 70 80 90 100
VKTFFQMKDQ LDNLLLKESL LEDFKGYLGC QALSEMIQFY LEEVMPQAEN
110 120 130 140 150
QDPDIKAHVN SLGENLKTLR LRLRRCHRFL PCENKSKAVE QVKNAFNKLQ
160 170
EKGIYKAMSE FDIFINYIEA YMTMKIRN
Length:178
Mass (Da):20,517
Last modified:August 1, 1991 - v1
Checksum:i6825E9FA4337CDE4
GO

Polymorphismi

A polymorphism in IL10 promoter region is associated with resistance to graft-versus-host disease (GVHDS) [MIMi:614395]. GVHDS is a major complication of allogeneic bone marrow transplantation, in which mature donor T-cells that contaminate the allogeneic bone marrow recognize the tissues of the recipient as foreign, causing a severe inflammatory disease.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01588315G → R in a family affected by Crohn disease; decreases secretion thereby reducing the anti-inflammatory effect. 1 PublicationCorresponds to variant rs145922845dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57627 mRNA. Translation: AAA63207.1.
U16720 Genomic DNA. Translation: AAA80104.1.
AY029171 mRNA. Translation: AAK38162.1.
AF418271 Genomic DNA. Translation: AAL06594.1.
CCDSiCCDS1467.1.
PIRiA38580.
RefSeqiNP_000563.1. NM_000572.2.
UniGeneiHs.193717.

Genome annotation databases

EnsembliENST00000423557; ENSP00000412237; ENSG00000136634.
GeneIDi3586.
KEGGihsa:3586.

Cross-referencesi

Web resourcesi

Wikipedia

Interleukin-10 entry

SeattleSNPs
SHMPD

The Singapore human mutation and polymorphism database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57627 mRNA. Translation: AAA63207.1.
U16720 Genomic DNA. Translation: AAA80104.1.
AY029171 mRNA. Translation: AAK38162.1.
AF418271 Genomic DNA. Translation: AAL06594.1.
CCDSiCCDS1467.1.
PIRiA38580.
RefSeqiNP_000563.1. NM_000572.2.
UniGeneiHs.193717.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ILKX-ray1.80A28-178[»]
1INRX-ray2.00A19-178[»]
1J7VX-ray2.90L19-178[»]
1LK3X-ray1.91A/B26-178[»]
1Y6KX-ray2.52L19-178[»]
2H24X-ray2.00A19-178[»]
2ILKX-ray1.60A19-178[»]
ProteinModelPortaliP22301.
SMRiP22301.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109800. 3 interactors.
DIPiDIP-3511N.
IntActiP22301. 7 interactors.
MINTiMINT-206740.
STRINGi9606.ENSP00000412237.

Polymorphism and mutation databases

BioMutaiIL10.
DMDMi124292.

Proteomic databases

PaxDbiP22301.
PeptideAtlasiP22301.
PRIDEiP22301.

Protocols and materials databases

DNASUi3586.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000423557; ENSP00000412237; ENSG00000136634.
GeneIDi3586.
KEGGihsa:3586.

Organism-specific databases

CTDi3586.
DisGeNETi3586.
GeneCardsiIL10.
HGNCiHGNC:5962. IL10.
HPAiCAB013120.
MalaCardsiIL10.
MIMi124092. gene.
614395. phenotype.
neXtProtiNX_P22301.
OpenTargetsiENSG00000136634.
Orphaneti238569. Autosomal recessive early-onset inflammatory bowel disease.
117. Behcet disease.
536. Systemic lupus erythematosus.
PharmGKBiPA29778.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IYFW. Eukaryota.
ENOG4111PXC. LUCA.
GeneTreeiENSGT00510000048535.
HOGENOMiHOG000072692.
HOVERGENiHBG004110.
InParanoidiP22301.
KOiK05443.
OMAiPHMLREL.
OrthoDBiEOG091G0Y3M.
PhylomeDBiP22301.
TreeFamiTF333253.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000136634-MONOMER.
SignaLinkiP22301.
SIGNORiP22301.

Miscellaneous databases

EvolutionaryTraceiP22301.
GeneWikiiInterleukin_10.
GenomeRNAii3586.
PROiP22301.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000136634.
CleanExiHS_IL10.
ExpressionAtlasiP22301. baseline and differential.
GenevisibleiP22301. HS.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR000098. IL-10.
IPR020443. IL-10/19/20/22/24/26_fam.
IPR020423. IL-10_CS.
[Graphical view]
PANTHERiPTHR11585. PTHR11585. 1 hit.
PfamiPF00726. IL10. 1 hit.
[Graphical view]
PRINTSiPR01294. INTRLEUKIN10.
SMARTiSM00188. IL10. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00520. INTERLEUKIN_10. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIL10_HUMAN
AccessioniPrimary (citable) accession number: P22301
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: November 30, 2016
This is version 175 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.