Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P22301

- IL10_HUMAN

UniProt

P22301 - IL10_HUMAN

Protein

Interleukin-10

Gene

IL10

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

    GO - Molecular functioni

    1. cytokine activity Source: BHF-UCL
    2. growth factor activity Source: UniProtKB
    3. interleukin-10 receptor binding Source: UniProtKB

    GO - Biological processi

    1. B cell differentiation Source: UniProtKB
    2. B cell proliferation Source: UniProtKB
    3. branching involved in labyrinthine layer morphogenesis Source: Ensembl
    4. cell-cell signaling Source: UniProtKB
    5. cellular response to estradiol stimulus Source: Ensembl
    6. cellular response to lipopolysaccharide Source: BHF-UCL
    7. cytoplasmic sequestering of NF-kappaB Source: UniProtKB
    8. defense response to bacterium Source: Ensembl
    9. hemopoiesis Source: UniProtKB
    10. inflammatory response Source: BHF-UCL
    11. leukocyte chemotaxis Source: ProtInc
    12. negative regulation of apoptotic process Source: UniProtKB
    13. negative regulation of B cell proliferation Source: MGI
    14. negative regulation of chronic inflammatory response to antigenic stimulus Source: Ensembl
    15. negative regulation of cytokine secretion involved in immune response Source: BHF-UCL
    16. negative regulation of growth of symbiont in host Source: Ensembl
    17. negative regulation of interferon-alpha biosynthetic process Source: UniProtKB
    18. negative regulation of interferon-gamma production Source: Ensembl
    19. negative regulation of interleukin-12 production Source: Ensembl
    20. negative regulation of interleukin-6 production Source: BHF-UCL
    21. negative regulation of membrane protein ectodomain proteolysis Source: BHF-UCL
    22. negative regulation of MHC class II biosynthetic process Source: UniProtKB
    23. negative regulation of myeloid dendritic cell activation Source: Ensembl
    24. negative regulation of nitric oxide biosynthetic process Source: Ensembl
    25. negative regulation of T cell proliferation Source: UniProtKB
    26. negative regulation of tumor necrosis factor biosynthetic process Source: Ensembl
    27. negative regulation of tumor necrosis factor production Source: Ensembl
    28. positive regulation of B cell apoptotic process Source: MGI
    29. positive regulation of cytokine secretion Source: BHF-UCL
    30. positive regulation of MHC class II biosynthetic process Source: Ensembl
    31. positive regulation of sequence-specific DNA binding transcription factor activity Source: BHF-UCL
    32. positive regulation of transcription, DNA-templated Source: BHF-UCL
    33. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    34. receptor biosynthetic process Source: BHF-UCL
    35. regulation of gene expression Source: MGI
    36. regulation of isotype switching Source: UniProtKB
    37. regulation of sensory perception of pain Source: Ensembl
    38. response to activity Source: Ensembl
    39. response to carbon monoxide Source: Ensembl
    40. response to drug Source: Ensembl
    41. response to glucocorticoid Source: BHF-UCL
    42. response to inactivity Source: Ensembl
    43. response to insulin Source: Ensembl
    44. response to molecule of bacterial origin Source: UniProtKB
    45. type 2 immune response Source: UniProtKB

    Keywords - Molecular functioni

    Cytokine

    Enzyme and pathway databases

    SignaLinkiP22301.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Interleukin-10
    Short name:
    IL-10
    Alternative name(s):
    Cytokine synthesis inhibitory factor
    Short name:
    CSIF
    Gene namesi
    Name:IL10
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:5962. IL10.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: BHF-UCL

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Keywords - Diseasei

    Disease mutation

    Organism-specific databases

    MIMi614395. phenotype.
    Orphaneti238569. Autosomal recessive early-onset inflammatory bowel disease.
    117. Behcet disease.
    206. Crohn disease.
    536. Systemic lupus erythematosus.
    PharmGKBiPA29778.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 18181 PublicationAdd
    BLAST
    Chaini19 – 178160Interleukin-10PRO_0000015360Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi30 ↔ 1261 Publication
    Disulfide bondi80 ↔ 1321 Publication
    Glycosylationi134 – 1341N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP22301.
    PRIDEiP22301.

    PTM databases

    PhosphoSiteiP22301.

    Expressioni

    Tissue specificityi

    Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

    Gene expression databases

    ArrayExpressiP22301.
    BgeeiP22301.
    CleanExiHS_IL10.
    GenevestigatoriP22301.

    Organism-specific databases

    HPAiCAB013120.

    Interactioni

    Subunit structurei

    Homodimer.2 Publications

    Protein-protein interaction databases

    BioGridi109800. 3 interactions.
    DIPiDIP-3511N.
    IntActiP22301. 2 interactions.
    MINTiMINT-206740.
    STRINGi9606.ENSP00000412237.

    Structurei

    Secondary structure

    1
    178
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni33 – 364
    Helixi37 – 4913
    Helixi52 – 587
    Helixi68 – 758
    Turni76 – 783
    Helixi79 – 9315
    Helixi95 – 995
    Turni103 – 1053
    Helixi106 – 12520
    Helixi127 – 1293
    Helixi131 – 1333
    Helixi137 – 14812
    Helixi151 – 1599
    Helixi161 – 17616

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ILKX-ray1.80A28-178[»]
    1INRX-ray2.00A19-178[»]
    1J7VX-ray2.90L19-178[»]
    1LK3X-ray1.91A/B26-178[»]
    1Y6KX-ray2.52L19-178[»]
    2H24X-ray2.00A19-178[»]
    2ILKX-ray1.60A19-178[»]
    ProteinModelPortaliP22301.
    SMRiP22301. Positions 24-178.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP22301.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the IL-10 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG43085.
    HOGENOMiHOG000072692.
    HOVERGENiHBG004110.
    InParanoidiP22301.
    KOiK05443.
    OMAiPHMLREL.
    OrthoDBiEOG7C2R33.
    PhylomeDBiP22301.
    TreeFamiTF333253.

    Family and domain databases

    Gene3Di1.20.1250.10. 1 hit.
    InterProiIPR009079. 4_helix_cytokine-like_core.
    IPR012351. 4_helix_cytokine_core.
    IPR000098. IL-10.
    IPR020443. IL-10/19/20/24/26_fam.
    IPR020423. IL-10_CS.
    [Graphical view]
    PANTHERiPTHR11585. PTHR11585. 1 hit.
    PfamiPF00726. IL10. 1 hit.
    [Graphical view]
    PRINTSiPR01294. INTRLEUKIN10.
    SMARTiSM00188. IL10. 1 hit.
    [Graphical view]
    SUPFAMiSSF47266. SSF47266. 1 hit.
    PROSITEiPS00520. INTERLEUKIN_10. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P22301-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHSSALLCCL VLLTGVRASP GQGTQSENSC THFPGNLPNM LRDLRDAFSR    50
    VKTFFQMKDQ LDNLLLKESL LEDFKGYLGC QALSEMIQFY LEEVMPQAEN 100
    QDPDIKAHVN SLGENLKTLR LRLRRCHRFL PCENKSKAVE QVKNAFNKLQ 150
    EKGIYKAMSE FDIFINYIEA YMTMKIRN 178
    Length:178
    Mass (Da):20,517
    Last modified:August 1, 1991 - v1
    Checksum:i6825E9FA4337CDE4
    GO

    Polymorphismi

    A polymorphism in IL10 promoter region is associated with resistance to graft-versus-host disease (GVHDS) [MIMi:614395]. GVHDS is a major complication of allogeneic bone marrow transplantation, in which mature donor T-cells that contaminate the allogeneic bone marrow recognize the tissues of the recipient as foreign, causing a severe inflammatory disease.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti15 – 151G → R in a family affected by Crohn disease; decreases secretion thereby reducing the anti-inflammatory effect. 1 Publication
    VAR_015883

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M57627 mRNA. Translation: AAA63207.1.
    U16720 Genomic DNA. Translation: AAA80104.1.
    AY029171 mRNA. Translation: AAK38162.1.
    AF418271 Genomic DNA. Translation: AAL06594.1.
    CCDSiCCDS1467.1.
    PIRiA38580.
    RefSeqiNP_000563.1. NM_000572.2.
    UniGeneiHs.193717.

    Genome annotation databases

    EnsembliENST00000423557; ENSP00000412237; ENSG00000136634.
    GeneIDi3586.
    KEGGihsa:3586.
    UCSCiuc001hen.1. human.

    Polymorphism databases

    DMDMi124292.

    Cross-referencesi

    Web resourcesi

    Wikipedia

    Interleukin-10 entry

    SeattleSNPs
    SHMPD

    The Singapore human mutation and polymorphism database

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M57627 mRNA. Translation: AAA63207.1 .
    U16720 Genomic DNA. Translation: AAA80104.1 .
    AY029171 mRNA. Translation: AAK38162.1 .
    AF418271 Genomic DNA. Translation: AAL06594.1 .
    CCDSi CCDS1467.1.
    PIRi A38580.
    RefSeqi NP_000563.1. NM_000572.2.
    UniGenei Hs.193717.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ILK X-ray 1.80 A 28-178 [» ]
    1INR X-ray 2.00 A 19-178 [» ]
    1J7V X-ray 2.90 L 19-178 [» ]
    1LK3 X-ray 1.91 A/B 26-178 [» ]
    1Y6K X-ray 2.52 L 19-178 [» ]
    2H24 X-ray 2.00 A 19-178 [» ]
    2ILK X-ray 1.60 A 19-178 [» ]
    ProteinModelPortali P22301.
    SMRi P22301. Positions 24-178.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109800. 3 interactions.
    DIPi DIP-3511N.
    IntActi P22301. 2 interactions.
    MINTi MINT-206740.
    STRINGi 9606.ENSP00000412237.

    PTM databases

    PhosphoSitei P22301.

    Polymorphism databases

    DMDMi 124292.

    Proteomic databases

    PaxDbi P22301.
    PRIDEi P22301.

    Protocols and materials databases

    DNASUi 3586.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000423557 ; ENSP00000412237 ; ENSG00000136634 .
    GeneIDi 3586.
    KEGGi hsa:3586.
    UCSCi uc001hen.1. human.

    Organism-specific databases

    CTDi 3586.
    GeneCardsi GC01M206940.
    HGNCi HGNC:5962. IL10.
    HPAi CAB013120.
    MIMi 124092. gene.
    614395. phenotype.
    neXtProti NX_P22301.
    Orphaneti 238569. Autosomal recessive early-onset inflammatory bowel disease.
    117. Behcet disease.
    206. Crohn disease.
    536. Systemic lupus erythematosus.
    PharmGKBi PA29778.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG43085.
    HOGENOMi HOG000072692.
    HOVERGENi HBG004110.
    InParanoidi P22301.
    KOi K05443.
    OMAi PHMLREL.
    OrthoDBi EOG7C2R33.
    PhylomeDBi P22301.
    TreeFami TF333253.

    Enzyme and pathway databases

    SignaLinki P22301.

    Miscellaneous databases

    EvolutionaryTracei P22301.
    GeneWikii Interleukin_10.
    GenomeRNAii 3586.
    NextBioi 14011.
    PROi P22301.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P22301.
    Bgeei P22301.
    CleanExi HS_IL10.
    Genevestigatori P22301.

    Family and domain databases

    Gene3Di 1.20.1250.10. 1 hit.
    InterProi IPR009079. 4_helix_cytokine-like_core.
    IPR012351. 4_helix_cytokine_core.
    IPR000098. IL-10.
    IPR020443. IL-10/19/20/24/26_fam.
    IPR020423. IL-10_CS.
    [Graphical view ]
    PANTHERi PTHR11585. PTHR11585. 1 hit.
    Pfami PF00726. IL10. 1 hit.
    [Graphical view ]
    PRINTSi PR01294. INTRLEUKIN10.
    SMARTi SM00188. IL10. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47266. SSF47266. 1 hit.
    PROSITEi PS00520. INTERLEUKIN_10. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI."
      Vieira P., de Waal-Malefyt R., Dang M.-N., Johnson K.E., Kastelein R., Fiorentino D.F., Devries J.E., Roncarolo M.-G., Mosmann T.R., Moore K.W.
      Proc. Natl. Acad. Sci. U.S.A. 88:1172-1176(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: T-cell.
    2. "The structure of the human IL-10 gene."
      Sanjanwala B., de Waal-Malefyt R.
      Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Cloning, sequencing of human interleukin-10 cDNA and construction of its eukaryotic expression vector."
      Dai W.-J., Jiang H.-C., Pan S.-H.
      Haerbin Yi Ke Da Xue Xue Bao 35:4-6(2001)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. SeattleSNPs variation discovery resource
      Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. Cited for: PROTEIN SEQUENCE OF 26-34 AND 170-178.
    6. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
      Zhang Z., Henzel W.J.
      Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 19-33.
    7. "Disulfide bond assignments and secondary structure analysis of human and murine interleukin 10."
      Windsor W.T., Syto R., Tsarbopoulos A., Zhang R., Durkin J., Baldwin S., Paliwal S., Mui P.W., Pramanik B., Trotta P.P.
      Biochemistry 32:8807-8815(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISULFIDE BONDS.
    8. "Relation of an interleukin-10 promoter polymorphism to graft-versus-host disease and survival after hematopoietic-cell transplantation."
      Lin M.T., Storer B., Martin P.J., Tseng L.H., Gooley T., Chen P.J., Hansen J.A.
      N. Engl. J. Med. 349:2201-2210(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INVOLVEMENT IN RESISTANCE TO GVHDS.
    9. "Crystal structure of interleukin 10 reveals an interferon gamma-like fold."
      Walter M.R., Nagabhushan T.L.
      Biochemistry 34:12118-12125(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
    10. "Crystal structure of interleukin-10 reveals the functional dimer with an unexpected topological similarity to interferon gamma."
      Zdanov A., Schalk-Hihi C., Gustchina A., Tsang M., Wheatherbee J., Wlodawer A.
      Structure 3:591-601(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
    11. "Crystal structure of human interleukin-10 at 1.6-A resolution and a model of a complex with its soluble receptor."
      Zdanov A., Schalk-Hihi C., Wlodawer A.
      Protein Sci. 5:1955-1962(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
    12. "Crystal structure of the IL-10/IL-10R1 complex reveals a shared receptor binding site."
      Josephson K., Logsdon N.J., Walter M.R.
      Immunity 15:35-46(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 19-178 IN COMPLEX WITH IL10R1.
    13. "Same structure, different function crystal structure of the Epstein-Barr virus IL-10 bound to the soluble IL-10R1 chain."
      Yoon S.I., Jones B.C., Logsdon N.J., Walter M.R.
      Structure 13:551-564(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 19-178 IN COMPLEX WITH IL10R1.
    14. "A Gly15Arg mutation in the interleukin-10 gene reduces secretion of interleukin-10 in Crohn disease."
      van der Linde K., Boor P.P., Sandkuijl L.A., Meijssen M.A., Savelkoul H.F., Wilson J.H., de Rooij F.W.
      Scand. J. Gastroenterol. 38:611-617(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT ARG-15.

    Entry informationi

    Entry nameiIL10_HUMAN
    AccessioniPrimary (citable) accession number: P22301
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 155 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3